81,065 results

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GANLS

Plasmid

#50852

Purpose

Expresses one ddFP in mammalian cells, the second plasmid for a red to green colour switch-based fluorescent caspase-3 biosensor, used together with plasmid NESRA-DEVD-BNLS.

Depositor

Robert Campbell

Article

Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

Insert

ddGFP A

Tags

triplicated NLS sequence DPKKKRKV

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only
Tubulin-C-18

Plasmid

#58197

Purpose

Localization: Microtubules, Excitation: N/A, Emission: N/A

Depositor

Michael Davidson

Insert

Tubulin (TUBA1B Human)

Tags

SNAP tag

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 13, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pCMV5-25BoxB

Plasmid

#60817

Purpose

Cloning vector, 25 repeats of 19 bp BoxB sequence from bacteriophage-λ, for RNA labeling. BoxB-RNA is bound by 22 amino acid RNA binding domain of λ-anti-terminator protein N, e.g. tagged with GFP

Depositor

Maria Carmo-Fonseca

Article

Martin et al Cell Rep. 2013 Sep 26;4(6):1144-55. doi:

Insert

25x BoxB

Use

Synthetic Biology

Expression

Mammalian

Mutation

25 repeats of BoxB sequence

Promoter

CMV

Available Since

Nov. 16, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA-Flag-H3.3-polyA

Plasmid

#59780

Purpose

Express Flag-tagged H3.3 (H3F3B) in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

Depositor

Yi Zhang

Article

Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

Insert

H3F3B (H3-3B Human)

Expression

Mammalian

Promoter

CMV and T7

Available Since

Sept. 16, 2014

Availability

Academic Institutions and Nonprofits only

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piCRg Entry

Plasmid

#58904

Purpose

Cas9/gRNA Entry expression plasmid

Depositor

Danwei Huangfu

Article

Gonzalez et al Cell Stem Cell. 2014 Jun 11. pii: S1934-

Insert

hSpCas9

Expression

Mammalian

Available Since

Sept. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-nEF Con/Fon hChR2(H134R)-EYFP

Plasmid

#55644

Purpose

Cre-on/Flp-on ChR2-EYFP under the short Ef1a promoter

Depositor

Karl Deisseroth

Article

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Insert

ChR2(H134R)-EYFP

Use

AAV; Cre on/flp on chr2-eyfp

Tags

EYFP

Expression

Mammalian

Promoter

nEF

Available Since

Aug. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pGolden-Neo

Plasmid

#51422

Purpose

Contain one loxP-PGK-Neo-pA-loxP cassette, used for golden-gate reaction

Depositor

Yonglun Luo

Article

Luo et al Biotechniques. 2014 May 1;56(5):263-8. d

Insert

PGK1 phosphoglycerate kinase 1 (PGK1 Human)

Expression

Mammalian

Promoter

PGK

Available Since

March 21, 2014

Availability

Academic Institutions and Nonprofits only

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MR015-H11-R2-TALEN

Plasmid

#51555

Purpose

Expresses the H11-R2 TALEN in mammalian cells. To cleave the H11 locus, this TALEN was coexpressed with H11-L2 (plasmid #51554).

Depositor

Michele Calos

Article

Zhu et al Nucleic Acids Res. 2013 Dec 4.

Insert

H11-R2 TALEN

Use

TALEN

Expression

Mammalian

Available Since

March 4, 2014

Availability

Academic Institutions and Nonprofits only

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pLenti6.3-hEPO

Plasmid

#50436

Purpose

express human erythropoietin using lentiviral delivery system

Depositor

Juan Melero-Martin

Article

Lin et al Blood. 2011 Nov 17;118(20):5420-8. doi:

Insert

EPO (EPO Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only
pmCherry Vinculin

Plasmid

#50527

Depositor

Kenneth Yamada

Insert

Vinculin (VCL Chicken)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pU6_gRNA_handle_U6t

Plasmid

#49016

Purpose

gRNA expression empty vector (mammalian cells)

Depositor

Timothy Lu

Article

Farzadfard et al ACS Synth Biol. 2013 Sep 11.

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Available Since

Oct. 29, 2013

Availability

Academic Institutions and Nonprofits only

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pMXs-hLIN28A

Plasmid

#47902

Purpose

Expresses human LIN28A in mammalian cells using retrovirus system.

Depositor

Shinya Yamanaka

Article

Tanabe et al Proc Natl Acad Sci U S A. 2013 Jul 23;11

Insert

human LIN28A (LIN28A Human)

Use

Retroviral

Expression

Mammalian

Promoter

LTR

Available Since

Oct. 2, 2013

Availability

Academic Institutions and Nonprofits only

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mH2A1.1-CT-GFP

Plasmid

#45167

Depositor

Brian Chadwick, Hunt Willard

Article

Chadwick et al Hum Mol Genet. 2001 May 1;10(10):1101-13

Insert

H2Afy (MACROH2A1 Human)

Tags

GFP

Expression

Mammalian

Available Since

July 2, 2013

Availability

Academic Institutions and Nonprofits only

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pCMV-Klb-EGFP

Plasmid

#45531

Depositor

Zipora Yablonka-Reuveni

Article

Phelps et al FEBS J. 2016 May;283(9):1653-68. doi: 10

Insert

beta Klotho (Klb Mouse)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

June 18, 2013

Availability

Academic Institutions and Nonprofits only

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cfSGFP2-N

Plasmid

#37533

Depositor

Ikuo Wada

Article

Suzuki et al PLoS One. 2012;7(5):e37551. Epub 2012 Ma

Insert

cysteine-free SGFP2-N

Expression

Mammalian

Mutation

C48S and C70M mutations relative to SGFP2

Promoter

CMV

Available Since

Aug. 6, 2012

Availability

Academic Institutions and Nonprofits only

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pTK21

Plasmid

#37396

Depositor

Iain Cheeseman

Article

Kiyomitsu et al Nat Cell Biol. 2012 Feb 12;14(3):311-7.

Insert

Ran (RAN Human)

Tags

S-tag, TEV, and mCherry

Expression

Mammalian

Mutation

T24N

Available Since

July 19, 2012

Availability

Academic Institutions and Nonprofits only

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pSMP-EZH2_1

Plasmid

#36387

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

EZH2 (EZH2 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only

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Tagged-Cap

Plasmid

#35572

Depositor

Marian Waterman

Article

Tsai et al Mol Cell Proteomics. 2011 Apr;10(4):M110

Type

Empty backbone

Use

Luciferase

Tags

Ms2 Stem Loop

Expression

Mammalian

Promoter

SV40

Available Since

April 3, 2012

Availability

Academic Institutions and Nonprofits only

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pACAGW-H2B-PAGFP-AAV

Plasmid

#33000

Depositor

Massimo Scanziani

Article

Lien et al Front Neural Circuits. 2011;5:16. Epub 2

Insert

H2B-PAGFP (H2BC21 Human, Aequorea victoria)

Use

AAV; Adeno associated virus

Tags

PAGFP

Expression

Mammalian

Available Since

Dec. 12, 2011

Availability

Academic Institutions and Nonprofits only

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pCMMP-EnvA-IRES-GFP

Plasmid

#32206

Depositor

Edward Callaway

Article

Wickersham et al Neuron. 2007 Mar 1. 53(5):639-47.

Insert

EnvARGCD

Use

Retroviral

Expression

Mammalian

Available Since

Sept. 26, 2011

Availability

Academic Institutions and Nonprofits only
pLenti CMVTRE3G eGFP Neo (w821-1)

Plasmid

#27569

Depositor

Eric Campeau

Insert

Enhance Green Fluorescent Protein

Use

Lentiviral

Expression

Mammalian

Available Since

June 6, 2011

Availability

Academic Institutions and Nonprofits only

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FCHo1-pmCherryC1

Plasmid

#27690

Depositor

Christien Merrifield

Article

Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

Insert

FCHo1 (Fcho1 Mouse)

Tags

mCherry

Expression

Mammalian

Available Since

May 4, 2011

Availability

Academic Institutions and Nonprofits only
Flag-MALT1-/pRK5

Plasmid

#27971

Depositor

Xiaolu Yang

Article

Kawadler et al Mol Cell. 2008 Aug 8. 31(3):415-21.

Insert

MALT1 (MALT1 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 23, 2011

Availability

Academic Institutions and Nonprofits only
pLKO1-shYAP2

Plasmid

#27369

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2008 Jul 15. 22(14):1962-71.

Insert

shYAP2

Expression

Mammalian

Available Since

Feb. 2, 2011

Availability

Academic Institutions and Nonprofits only
pCAG-delta90GFP

Plasmid

#26645

Depositor

Anjen Chenn

Article

Wrobel et al Dev Biol. 2007 Sep 15. 309(2):285-97.

Insert

delta90 beta-catenin (Ctnnb1 Mouse)

Tags

GFP

Expression

Mammalian

Mutation

Deletion of first 90 aa of beta catenin

Available Since

Nov. 8, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA HA HP1 gamma

Plasmid

#24080

Depositor

Naoko Tanese

Article

Vassallo et al Proc Natl Acad Sci U S A. 2002 Apr 30. 9

Insert

HP1 gamma (CBX3 Human)

Tags

HA

Expression

Mammalian

Available Since

Oct. 27, 2010

Availability

Academic Institutions and Nonprofits only

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pFRT_TO_DESTFLAGHA

Plasmid

#26361

Depositor

Thomas Tuschl

Article

Spitzer et al Genes Chromosomes Cancer. 2011 May . 50(

Type

Empty backbone

Tags

FLAG/HA

Expression

Mammalian

Available Since

Sept. 22, 2010

Availability

Academic Institutions and Nonprofits only
HUW-TetO-hNanog

Plasmid

#25700

Depositor

Rudolf Jaenisch

Article

Hanna et al Nature. 2009 Dec 3. 462(7273):595-601.

Insert

Nanog (NANOG Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Sept. 16, 2010

Availability

Academic Institutions and Nonprofits only
pCMV5 WT KSR1

Plasmid

#25970

Depositor

Rob Lewis

Article

Joneson et al J Biol Chem. 1998 Mar 27. 273(13):7743-8

Insert

Kinase suppressor of Ras 1 (Ksr1 Mouse)

Tags

Flag

Expression

Mammalian

Available Since

Aug. 13, 2010

Availability

Academic Institutions and Nonprofits only

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pAAV-SEPT-Acceptor

Plasmid

#25648

Depositor

Todd Waldman

Article

Kim et al Nucleic Acids Res. 2008 Nov . 36(19):e12

Insert

0

Use

AAV

Expression

Mammalian

Available Since

July 14, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge

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