81,268 results

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GLUT1-eGFP/pcDNA-DEST47

Plasmid

#18729

Depositor

Wolf Frommer

Article

Takanaga et al Biochim Biophys Acta. 2008 Apr . 1778(4)

Insert

pcDNA-DEST47 /hGLUT1-eGFP

Tags

GFP

Expression

Mammalian

Mutation

deleted TAA

Available Since

July 3, 2008

Availability

Academic Institutions and Nonprofits only

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pWZL Blast Snail ER

Plasmid

#18798

Depositor

Bob Weinberg

Article

Mani et al Cell. 2008 May 16. 133(4):704-15.

Insert

snail (SNAI1 Human)

Use

Retroviral

Tags

ER hormone binding domain

Expression

Mammalian

Mutation

G525R mutation in the ER. The presence of the G52…

Available Since

July 3, 2008

Availability

Academic Institutions and Nonprofits only

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p2xFLAGhYAP1-S127A

Plasmid

#17790

Depositor

Marius Sudol

Article

Komuro et al J Biol Chem. 2003 Aug 29. 278(35):33334-

Insert

Yes-kinase associated protein mutant S127A (YAP1 Human)

Tags

2xFlag

Expression

Mammalian

Mutation

Serine 127 to Alanine

Available Since

May 9, 2008

Availability

Academic Institutions and Nonprofits only

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EF.hHES1.Ubc.GFP

Plasmid

#17624

Depositor

Linzhao Cheng

Article

Yu et al Stem Cells. 2006 Apr . 24(4):876-88.

Insert

HES1 (HES1 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

March 27, 2008

Availability

Academic Institutions and Nonprofits only

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pCMV-Myc CDC4 WT*

Plasmid

#16652

Depositor

Bert Vogelstein

Article

Rajagopalan et al Nature. 2004 Mar 4. 428(6978):77-81.

Insert

CDC4 (FBXW7 Human)

Tags

myc

Expression

Mammalian

Mutation

*Note that this insert is only WT relative to the…

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only

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MSCV PRDM16

Plasmid

#15504

Depositor

Bruce Spiegelman

Article

Seale et al Cell Metab. 2007 Jul . 6(1):38-54.

Insert

PRDM16 (isoform 4) (Prdm16 Mouse)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Available Since

Dec. 14, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA3-EGFP-Rac1(Q61L)

Plasmid

#13720

Depositor

Klaus Hahn

Article

Kraynov et al Science. 2000 Oct 13. 290(5490):333-7.

Insert

EGFP-Rac1(Q61L) (RAC1 Human)

Tags

EGFP

Expression

Mammalian

Available Since

March 28, 2007

Availability

Academic Institutions and Nonprofits only

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1245 pCMVb p300

Plasmid

#10717

Depositor

William Sellers

Insert

p300 (EP300 Human)

Expression

Mammalian

Available Since

Dec. 7, 2005

Availability

Academic Institutions and Nonprofits only

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pLVX-UbC-rtTA-Ngn2:2A:EGFP

Plasmid

#127288

Purpose

all-in-one inducible LV expression construct for Ngn2:2A:EGFP (aka. UNG; for direct neuronal conversion)

Depositor

Fred Gage

Article

Schafer et al Nat Neurosci. 2019 Feb;22(2):243-255. do

Insert

Ngn2:2A:Ascl1 (NEUROG2 Human)

Use

Lentiviral

Tags

Ngn2 and EGFP linked via 2A

Expression

Mammalian

Available Since

June 6, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP‐RNASEH1

Plasmid

#108699

Purpose

For expression of C-terminally eGFP-tagged human RNASEH1 (nuclear isoform) in mammalian cells

Depositor

Andrew Jackson, Martin Reijns

Article

Bubeck et al Nucleic Acids Res. 2011 May;39(9):3652-6

Insert

RNASEH1 (nuclear isoform) (RNASEH1 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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dCas9-hHDAC3

Plasmid

#98591

Purpose

3rd generation lenti vector encoding dCas9-hHDAC3 (EF1a-NLS-dCas9(N863)-hHDAC3). Expresses dCas9 fused to human HDAC3.

Depositor

Zhaolan Zhou

Article

Kwon et al Nat Commun. 2017 May 12;8:15315. doi: 10

Insert

dCAS9-HDAC3 (HDAC3 S. pyogenes, Human, Synthetic)

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D10A and N863A in Cas9

Promoter

EF1A

Available Since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pgRNA-modified

Plasmid

#84477

Purpose

guide RNA expression lentiviral construct

Depositor

Rudolf Jaenisch

Article

Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

Insert

guide RNA

Use

Lentiviral

Expression

Mammalian

Promoter

mU6

Available Since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1 EGFP-3XNLS

Plasmid

#58468

Purpose

Expresses a nuclear-targeted eGFP on a CMV promoter

Depositor

Dyche Mullins

Article

Belin et al Mol Biol Cell. 2013 Apr;24(7):982-94. do

Insert

3X NLS with 3X stop codons

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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pCAG-Kir2.1-T2A-tdTomato

Plasmid

#60598

Purpose

Expresses Kir2.1-T2A-tdTomato

Depositor

Massimo Scanziani

Article

Xue et al Nature. 2014 Jul 31;511(7511):596-600. d

Insert

Kir2.1-T2A-tdTomato (Kcnj2 Mouse)

Tags

myc

Expression

Mammalian

Available Since

Nov. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pB-GLUT4-7myc-GFP

Plasmid

#52872

Purpose

detection and measurement of GLUT4 translocation

Depositor

Jonathan Bogan

Article

Bogan et al Mol Cell Biol. 2001 Jul;21(14):4785-806.

Insert

GLUT4 (Slc2a4 Rat, with human c-terminus)

Use

Retroviral

Tags

7x-myc (internal) and GFP

Expression

Mammalian

Available Since

April 22, 2014

Availability

Academic Institutions and Nonprofits only

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pABpuro-BluF

Plasmid

#46824

Purpose

luciferase reporter for BmalI promoter

Depositor

Steven Brown

Article

Brown et al PLoS Biol. 2005 Oct;3(10):e338. Epub 200

Insert

Bmal1 (Bmal1 Mouse)

Use

Lentiviral and Luciferase

Tags

luciferase

Expression

Mammalian

Promoter

Bmal1

Available Since

July 29, 2013

Availability

Academic Institutions and Nonprofits only

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pRSI9-U6-(sh)-UbiC-TagRFP-2A-Puro

Plasmid

#28289

Purpose

3rd generation lentiviral plasmid; This plasmid is the backbone for the DECIPHER libraries (currently DISCONTINUED)

Depositor

Alex Chenchik, Gus Frangou

Type

Empty backbone

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

April 20, 2011

Availability

Academic Institutions and Nonprofits only

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GFP-cortactin

Plasmid

#26722

Depositor

Anna Huttenlocher

Article

Perrin et al Mol Biol Cell. 2006 Jan . 17(1):239-50.

Insert

Cortactin (Cttn Mouse)

Tags

EGFP

Expression

Mammalian

Available Since

Dec. 30, 2010

Availability

Academic Institutions and Nonprofits only

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XBP-1 1: pFLAG.XBP1u.CMV2

Plasmid

#21832

Depositor

David Ron

Article

Calfon et al Nature. 2002 Jan 3. 415(6867):92-6.

Insert

XBP-1 unprocessed (Xbp1 Mouse)

Tags

Flag

Expression

Mammalian

Available Since

Oct. 8, 2009

Availability

Academic Institutions and Nonprofits only

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pLenti PGK Blast DEST (w524-1)

Plasmid

#19065

Purpose

3rd gen lentiviral Gateway destination vector, expression, PGK promoter, Blast

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
EF.hICN1.Ubc.GFP

Plasmid

#17626

Depositor

Linzhao Cheng

Article

Yu et al Stem Cells. 2006 Apr . 24(4):876-88.

Insert

ICN1 (NOTCH1 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

March 27, 2008

Availability

Academic Institutions and Nonprofits only

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pCMV-FLAG LAP2

Plasmid

#15738

Depositor

Joan Massague

Article

Gomis et al Cancer Cell. 2006 Sep . 10(3):203-14.

Insert

LAP2 (CEBPB Human)

Tags

FLAG

Expression

Mammalian

Available Since

Sept. 7, 2007

Availability

Academic Institutions and Nonprofits only

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HA-Foxo1 (pCMV5)

Plasmid

#12142

Depositor

Domenico Accili

Article

Nakae et al J Clin Invest. 2001 Nov . 108(9):1359-67

Insert

Foxo1 (Foxo1 Mouse)

Tags

HA and Myc

Expression

Mammalian

Mutation

Wild-type

Promoter

CMV

Available Since

Aug. 2, 2007

Availability

Academic Institutions and Nonprofits only

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pJ3H-Mst1

Plasmid

#12203

Depositor

Jonathan Chernoff

Article

Creasy et al J Biol Chem. 1996 Aug 30. 271(35):21049-

Insert

Mst1 (STK4 Human)

Tags

HA

Expression

Mammalian

Available Since

Aug. 11, 2006

Availability

Academic Institutions and Nonprofits only

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pcDNA3 HA Arf6

Plasmid

#10834

Depositor

Thomas Roberts

Article

Furman et al J Biol Chem. 2002 Mar 8. 277(10):7962-9.

Insert

Arf6 (ARF6 Human)

Tags

HA

Expression

Mammalian

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
PB-UniSAM

Plasmid

#99866

Purpose

Encodes for Cas9-VP64, MS2-p65-HSF1, mCherry and for the gRNA 2.0

Depositor

Lesley Forrester

Article

Fidanza et al Sci Rep. 2017 Jul 25;7(1):6394. doi: 10.

Insert

UniSAM-mCherry + U6-gRNA2.0

Use

CRISPR and Synthetic Biology; Dcas9-sam activation

Expression

Mammalian

Mutation

BbsI sites in CDS were ablated by consensus mutat…

Promoter

EF1a

Available Since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-hAR

Plasmid

#89078

Purpose

mammalian expression of human androgen receptor in pCMV5

Depositor

Elizabeth Wilson

Article

Lubahn et al Science. 1988 Apr 15;240(4850):327-30.

Insert

human androgen receptor (hAR) full-length (AR Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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pCDNA3-HA-HA-humanCMYC

Plasmid

#74164

Purpose

Mammalian expression of human cMyc

Depositor

Martine Roussel

Article

Vo et al Cancer Cell. 2016 Jan 11;29(1):5-16. doi

Insert

cMyc (MYC Human)

Tags

2x HA

Expression

Mammalian

Promoter

CMV

Available Since

April 11, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pBABEpuro-gateway

Plasmid

#51070

Purpose

gateway adapted mammalian expression vector

Depositor

Matthew Meyerson

Article

Greulich et al Proc Natl Acad Sci U S A. 2012 Sep 4;109

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Promoter

SV40

Available Since

Feb. 25, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pQCXIH-Myc-YAP

Plasmid

#33091

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

Insert

YAP (YAP1 Human)

Use

Retroviral

Tags

Myc

Expression

Mammalian

Promoter

CMV/MSV

Available Since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge

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81,268 results