2,202 results
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pDest-Tol2 (Chien)-Tol2-Isl2b:GFP:polyA
Plasmid#105648PurposeExpression of GFP in sensory neurons (retinal ganglion cells, trigeminal, Rohon Beard neurons and dorsal root ganglia) for up to 2 weeksDepositorInsertTol2-Isl2b:GFP:polyA (isl2b Zebrafish)
ExpressionMammalianAvailable SinceFeb. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCS2FA CO Tol2 TPase
Plasmid#133032PurposeExpression vector for production of codon optimized Tol2 transposaseDepositorInsertCodon optimized Tol2 Transposase
UseIn vitro expressionMutationCodon OptimizedAvailable SinceJune 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
p5E-kdrl
Plasmid#78687Purposekdrl promoter for Gateway cloningDepositorAvailable SinceJune 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
slc1a3b:myrGCaMP6s-P2A-H2AmCherry
Plasmid#170209PurposeDrives myrGCaMP6s-P2A-H2AmCherry expression with the slc1a3b promoter in astrocytes. The myrGCaMP6s calcium indicator expressed in the membrane and mCherry labels the nucleusDepositorAvailable SinceJune 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
slc1a3b:myrGFP-P2A-H2AmCherry
Plasmid#170208PurposeDrives myrGFP-P2A-H2AmCherry expression with the slc1a3b promoter in astrocytes. The myrGFP labels the membrane and mCherry labels the nucleusDepositorAvailable SinceJune 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pME-cas9-2A-mCherry
Plasmid#107592PurposeAn entry vector with cas9 -2A-mCherryDepositorInsertcas9-2A-mCherry
UseCRISPRTags2A-mCherryAvailable SinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDESTtol2pACrymCherry
Plasmid#64023PurposeGateway destination vector with zebrafish aA-crystallin promoter driving mCherry GFP in lensDepositorInsertmCherry
UseGateway CloningPromoteralpha-Acrystallin promoterAvailable SinceMay 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTol2-Ubi-zFucci-pA-exorh-GFP (JDW 1489)
Plasmid#242593PurposeA Tol2 expression vector containing the zebrafish Ubi promoter driving zFUCCI to label cell cycle state. Exorh-EGFP in the backbone to label pineal cells.DepositorInsertmCerulean-zGeminin, mCherry-zCdt1
UseZebrafish expressioPromoterzebrafish UbiAvailable SinceApril 8, 2026AvailabilityAcademic Institutions and Nonprofits only -
pMT-sox10-NLS-BirA-2A-mCherry_Ras
Plasmid#80063PurposeSox10 promoter driving HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequencesDepositorInsertBiotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
UseZebrafish biotaggingTags3x HAExpressionBacterialPromoterSox10 promoterAvailable SinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
p5E-2.3k-fabp2
Plasmid#84927Purpose–2.3kb promoter of zebrafish fabp2/ifabp gene in Gateway 5’ Entry vectorDepositorAvailable SinceNov. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCSCherryN5icd
Plasmid#22456DepositorAvailable SinceDec. 10, 2009AvailabilityAcademic Institutions and Nonprofits only -
pENTR5'_ubi
Plasmid#27320DepositorInsertzebrafish ubiquitin promoter (ubb Zebrafish)
UseMultisite gateway 5' entry vectorAvailable SinceFeb. 3, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCS2.aBTX
Plasmid#122257PurposeExpress membrane-tethered alpha-bungarotoxin in Danio rerioDepositorInsertalpha-bungarotoxin
UseSynthetic BiologyTagsFLAGAvailable SinceFeb. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
bactin2:loxP-STOP-loxP-DsRed-express
Plasmid#68797PurposeFor making transgenic fishDepositorInsertsbeta-actin promoter
loxp-stop-codon-loxp-dsred
Available SinceDec. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDEST mfap4:turquoise2
Plasmid#135218PurposeCyan tagged macrophagesDepositorInsertmfap4 promoter and mTurquoise2
UseZebrafish expressionAvailable SinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pXTm-zebrafish Tspan4a
Plasmid#210327PurposeExpresses Tetraspanin-4a (TSPAN4a) as migrasome markerDepositorInsertTetraspanin 4a (tspan4a Zebrafish)
UseFish expressionAvailable SinceJan. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
ubbr:GCaMP8m
Plasmid#232485PurposeTo make fish transgenic lines that have calcium sensor expression in the whole body of the fishDepositorInsertjGCaMP8m
UseZebrafish expressionPromoterubbrAvailable SinceAug. 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
p5E-mpeg1.1
Plasmid#75023PurposeCan be used to drive expression in zebrafish microglia.DepositorInsertmacrophage expressed 1, tandem duplicate 1 (mpeg1.1 Zebrafish)
Use5' entry vector for multisite gateway three …Promotermpeg1.1Available SinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
hsp70l-loxP-mCherry-STOP-loxP-H2B-GFP_cryaa-cerulean
Plasmid#24334DepositorInsertH2B
UseCre/LoxTagsGFPExpressionWormMutationmCherry driven by an HSP70 promoterCerulean drive…Available SinceApril 27, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCS2(+)-zGrad
Plasmid#119716PurposePlasmid to generate zGrad mRNA to inactivate proteins in zebrafishDepositorInsertzGrad
ExpressionBacterialAvailable SinceJan. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMT-zic2a-cytoBirA-2A-mCherry_Ras
Plasmid#80064PurposeZic2a promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequencesDepositorInsertBiotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
UseZebrafish biotaggingTags3x HAExpressionBacterialPromoterZic2a enhancer driving c-fos promoterAvailable SinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
p5E-gfap
Plasmid#82401PurposeGateway-compatible zebrafish gfap promoterDepositorInsertgfap promoter
Available SinceNov. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTol2CG2-ubi:QFGal4-SV40pA
Plasmid#155119PurposeTol2 vector for ubiquitous expression of QFGal4. Contains cmlc2:mRFP-SV40pA transgenesis markerDepositorInsertubi:QFGal4 (ubb Zebrafish, Neurospora crassa, Budding Yeast)
UseZebrafish expressionPromoterubiquitin BAvailable SinceSept. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCS2-Opto-zFGFR1a
Plasmid#232639PurposeZebrafish FGFR1a receptor kinase domain fused to VfLOVDepositorInsertzFGFR1a kinase domain + VfLOV domain
Available SinceApril 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pXL010-Wnt dual (GFP-Fire) reporter
Plasmid#40588DepositorInsertWnt reporter sequences
UseLentiviralExpressionMammalianPromoterWnt promoterAvailable SinceMay 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCS2-Opto-Acvr1ba-P2A-Acvr2ba
Plasmid#232640PurposeOptogenetic Nodal activators from Sako et al. 2016 (Cell Reports) separated by a P2A peptide for singe-transcript expressionDepositorInsertAcvr1ba + VfLOV domain and Acvr2ba + VfLOV domain
Available SinceApril 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
Tol2-kdrl:mCherry-caax
Plasmid#194287PurposeExpression plasmid for zebrafish or Danionella cerebrumDepositorInsertkdrl promoter from Addgene plasmid # 78687 (kdrl Zebrafish)
UseZebrafish or danionella cerebrum expressionTagsmCherryCAAX from the Tol2kit (Kwan et al., 2007)Promoterkdrl(flk1)Available SinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST mfap4:icre-p2a-tomato
Plasmid#135217PurposeCre coexpressed with tdTomato in macrophagesDepositorInsertmfap4 promoter with iCre and Tomato
UseCre/Lox; Zebrafish expressionAvailable SinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2pA2_ubi:EGFP
Plasmid#27323DepositorInsertubiquitin:EGFP reporter
UseTol2 transposonAvailable SinceFeb. 3, 2011AvailabilityAcademic Institutions and Nonprofits only -
pUAS:Cas9T2AGFP;U6:sgRNA1;U6:sgRNA2
Plasmid#74009PurposeTissue-specific knock-out in zebrafish, Cas9 and GFP driven by a UAS promoterDepositorInserts5x UAS
Cas9
T2A
GFP
U6 promoter
UseCRISPRAvailable SinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
Tol2-elavl3-GCaMP6s
Plasmid#59531PurposeExpressed GCaMP6-slow, a calcium indicator, in neurons in zebrafish larva. The plasmid is in Tol2 transposon backboneDepositorInsertsAvailable SinceSept. 12, 2014AvailabilityAcademic Institutions and Nonprofits only -
p5E-elavl3
Plasmid#75025PurposeCan be used to drive pan neuronal expression in zebrafish.DepositorInsertELAV like neuron-specific RNA binding protein 3 (elavl3 Zebrafish)
Use5' entry vector for multisite gateway three …Promoterelavl3Available SinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMT-myl7-cytoBirA-2A-mCherry_Ras
Plasmid#80060PurposeMyl7 promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequencesDepositorInsertBiotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
UseZebrafish biotaggingTags3x HAExpressionBacterialPromoterMyl7 promoterAvailable SinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2pACryGFP
Plasmid#64022PurposeGateway destination vector with zebrafish aA-crystallin promoter driving GFP in lensDepositorInsertGFP
UseGateway CloningPromoteralpha-Acrystallin promoterAvailable SinceMay 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTol2-HuC(elavl3)-CaMPARI2
Plasmid#137185Purposepan-neuronal expression of CaMPARI2 in zebrafish, used to generate the CaMPARI2 zebrafish line at ZIRC (ZL13801)DepositorInsertHuC(elavl3)-CaMPARI2-FLAG-HA-myc-polyA
UseTol2 plasmid for zebrafishTagsFLAG, HA, mycPromoterHuC(elavl3)Available SinceMarch 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
p5E-gfap
Plasmid#75024PurposeCan be used to drive expression in zebrafish astrocytes.DepositorInsertglial fibrillary acidic protein promoter (gfap Zebrafish)
Use5' entry vector for multisite gateway three …PromotergfapAvailable SinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pubb-QFDBD-2x AD*-VP16*-EcR, α-cry:EGFP
Plasmid#180475PurposeInducible gene expression vector pubb-QFDBD-2x AD*-VP16*-EcR, α-cry:EGFPDepositorInsertZebrafish ubb promoter
UseSynthetic BiologyAvailable SinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
14UAS zfPSD95:GFP
Plasmid#74314PurposeA fusion of zebrafish PSD95 to GFP under control of 14 UAS repeatsDepositorInsertzebrafish PSD95
TagsEGFPMutationY599H and I793S (please see depositor comment bel…PromoterUASAvailable SinceMay 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ Integrin beta 1b-mKate
Plasmid#105975Purposein vitro transcription, Full length zebrafish Integrin beta 1b tagged C terminally with mKate2 using Gateway cloning.DepositorInsertIntegrinbeta1b
TagsmKateExpressionBacterialAvailable SinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only
