161,398 results

1. HA-Ubiquitin

   Plasmid

   #18712

   Purpose

   Mammalian expression of human ubiquitin with HA tag

   Depositor

   Edward Yeh

   Article

   Kamitani et al J Biol Chem. 1997 Nov 7. 272(45):28557-6

   Insert

   ubiquitin (UBB Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   The HA-Ubiquitin insert is from the UBB gene. The…

   Promoter

   Available since

   June 20, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-CBE4max-SpRY-P2A-EGFP (RTW5133)

   Plasmid

   #139999

   Purpose

   CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Walton et al Science. 2020 Apr 17;368(6488):290-296.

   Insert

   human codon optimized CBE4max SpCas9 variant named SpRY with P2A-EGFP
   

   Use

   CRISPR

   Tags

   P2A-EGFP

   Expression

   Mammalian

   Mutation

   nSpCas9=D10A; SpRY=A61R/L1111R/D1135L/S1136W/G121…

   Promoter

   CAG

   Available since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFGET19_Ulp1

   Plasmid

   #64697

   Purpose

   H6-Ulp1 from pHYRS52 into pET-28b(+)

   Depositor

   Hideo Iwai

   Article

   Guerrero et al Protein Expr Purif. 2015 Aug 20. pii: S1

   Insert

   ULP1 (ULP1 Budding Yeast, Saccharomyces cerevisiae)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Ulp1(403-621)

   Promoter

   T7

   Available since

   Sept. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMXs-3XHA-EGFP-OMP25

   Plasmid

   #83356

   Purpose

   For tagging mitochondria with HA epitopes (HA-MITO construct)

   Depositor

   David Sabatini

   Article

   Chen et al Cell. 2016 Aug 25;166(5):1324-1337.e11.

   Insert

   3XHA-EGFP-OMP25
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRSETB/mStayGold

   Plasmid

   #212017

   Purpose

   mStayGold has an n1 adaptor at its N-terminus and is useful for C-terminal tagging. This construct is used also for expression of mStayGold itself.

   Depositor

   Atsushi Miyawaki

   Article

   Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

   Insert

   mStayGold
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. HPV16 E7 TCR

   Plasmid

   #122728

   Purpose

   Express HPV16 E7 TCR

   Depositor

   Christian Hinrichs

   Article

   Jin et al JCI Insight. 2018 Apr 19;3(8). pii: 9948

   Insert

   HPV16 E7 TCR
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. OpenCRISPR-1

   Plasmid

   #221565

   Purpose

   Express OpenCRISPR-1 nuclease in human cells using a CMV promoter

   Depositor

   Ali Madani

   Article

   Ruffolo et al Nature (2025).

   Insert

   OpenCRISPR-1
   

   Use

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 28, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. rAAV2-retro helper

   Plasmid

   #81070

   Purpose

   retrograde AAV helper vector, carrying rep and modified capsid gene

   Depositor

   Alla Karpova, David Schaffer

   Article

   Tervo et al Neuron. 2016 Oct 6. pii: S0896-6273(16)3

   Inserts

   rep
   rAAV2-retro cap
   

   Use

   AAV and Unspecified; Helper vector

   Tags

   Expression

   Mutation

   Pro2Ala, possibly Asp613Gly and changes made to A…

   Promoter

   Available since

   Aug. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti-puro

   Plasmid

   #39481

   Purpose

   Tetracycline inducible 3rd generation lentiviral vector

   Depositor

   Ie-Ming Shih

   Article

   Guan et al Cancer Res. 2011 Nov 1;71(21):6718-27. E

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   V5 and 6XHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 13, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCS2+HyPer7-NES

    Plasmid

    #136467

    Purpose

    Mammalian expression of cytoplasm targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

    Depositor

    Vsevolod Belousov

    Article

    Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

    Insert

    HyPer7
    

    Use

    Tags

    Nuclear export signal from the HIV Rev protein

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, SP6

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHAGE-3FLAG-EEA1

    Plasmid

    #176491

    Purpose

    For lentiviral transduction of 3FLAG-EEA1

    Depositor

    Wade Harper

    Article

    Park et al Nat Commun. 2022 Oct 16;13(1):6112. doi:

    Insert

    EEA1 (EEA1 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    Available since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. gRNA-CasRx_SD1-pSV40-TagBFP

    Plasmid

    #221004

    Purpose

    Transiently express CasRx DR30 repeat with gRNA spacer. Contains SD1 mutation to enhance efficiency. SV40-TagBFP cassette to monitor transfection efficiency. Use BbsI with overhangs Fw-AAAC, Rv-AAAA.

    Depositor

    Mikko Taipale, Benjamin Blencowe

    Article

    Li et al Mol Cell. 2024 Jul 11;84(13):2573-2589.e

    Insert

    CasRx 30nt processed direct repeat with SD1 mutation
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    DR1 A>T

    Promoter

    U6

    Available since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSBbi-GP

    Plasmid

    #60511

    Purpose

    SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: GFP and puromycin resistance gene

    Depositor

    Eric Kowarz

    Article

    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

    Type

    Empty backbone

    Use

    Transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a/RPBSA

    Available since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLminP_Luc2P_RE17

    Plasmid

    #90359

    Purpose

    NFAT - PKC/Ca++ gene reporter

    Depositor

    Ramnik Xavier

    Article

    O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

    Insert

    Firefly luciferase
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    NFAT

    Available since

    Aug. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-GFP

    Plasmid

    #11153

    Purpose

    Mammalian expression vector for expression of GFP (CMV promoter)

    Depositor

    Connie Cepko

    Article

    Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 10

    Has Service

    Cloning Grade DNA

    Insert

    CMV promoter-EGFP
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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16. EF1a-dCasRx-RBM25

    Plasmid

    #221001

    Purpose

    Transient expression of dCasRx-RBM25. EF1a promoter.

    Depositor

    Mikko Taipale, Benjamin Blencowe

    Article

    Li et al Mol Cell. 2024 Jul 11;84(13):2573-2589.e

    Insert

    dCasRx-RBM25
    

    Use

    CRISPR

    Tags

    NLS and NLS-HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1-GFP(1-10)

    Plasmid

    #70219

    Purpose

    Expresses GFP(1-10) in mammalian cells

    Depositor

    Bo Huang

    Article

    Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

    Has Service

    Cloning Grade DNA

    Insert

    GFP(1-10)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMito-iRFP670

    Plasmid

    #45462

    Depositor

    Vladislav Verkhusha

    Article

    Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

    Insert

    iRFP670
    

    Use

    Tags

    human cytochrome C oxidase subunit VIII

    Expression

    Mammalian

    Mutation

    T2A, S45T, V116I, D124E, M170L, K180M, D202L, I20…

    Promoter

    CMV

    Available since

    June 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. lentiCas9-EGFP

    Plasmid

    #63592

    Purpose

    Expresses human codon-optimized S. pyogenes Cas9 protein and EGFP from EFS promoter. 3rd generation lentiviral backbone.

    Depositor

    Feng Zhang, Phil Sharp

    Article

    Chen et al Cell. 2015 Mar 12;160(6):1246-60. doi: 1

    Inserts

    Cas9
    EGFP
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG and P2A

    Expression

    Mammalian

    Mutation

    Promoter

    EFS-NS

    Available since

    April 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLX304_CMV::mCherry-LgBiT-FLAG-NES

    Plasmid

    #199715

    Purpose

    Expresses LgBiT, fused to mCherry, in the cytosol of mammalian cells. Can be used in generating stable cell lines via lentiviral transduction.

    Depositor

    Alice Ting

    Article

    Roh et al J Am Chem Soc. 2023 May 10;145(18):10220

    Insert

    mCherry-LgBiT-FLAG-NES
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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21. pAAV2/1

    Plasmid

    #112862

    Purpose

    AAV packaging plasmid expressing Rep/Cap genes

    Depositor

    James M. Wilson

    Insert

    Rep2/Cap1
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Truncated P5

    Available since

    Aug. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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22. eeBxb1

    Plasmid

    #222339

    Purpose

    Plasmid for expressing evolved and engineered Bxb1 in the mammalian cells

    Depositor

    David Liu

    Article

    Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103

    Insert

    Codon-optimized evolved and engineered Bxb1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. tdTomato-N1

    Plasmid

    #54642

    Purpose

    Localization: N1 Cloning Vector, Excitation: 554, Emission: 581

    Depositor

    Michael Davidson, Roger Tsien, Nathan Shaner

    Article

    Shaner et al Nat Biotechnol. 2004 Dec;22(12):1567-72.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. NG-ABE8e

    Plasmid

    #138491

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

    Insert

    ecTadA(8e)-nSpCas9 (NG)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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25. pKLV-U6gRNA(BbsI)-PGKpuro2ABFP

    Plasmid

    #50946

    Purpose

    An empty gRNA expression vector

    Depositor

    Kosuke Yusa

    Article

    Koike-Yusa et al Nat Biotechnol. 2014 Mar;32(3):267-73. d

    Insert

    None
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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26. pUMVC

    Plasmid

    #8449

    Purpose

    Packaging plasmid for producing MuLV retroviral particles. Use in conjunction with an envelope plasmid such as pCMV-VSV-G.

    Depositor

    Bob Weinberg

    Article

    Stewart et al RNA 2003 Apr;9(4):493-501.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 4, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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27. pLV-mCherry

    Plasmid

    #36084

    Purpose

    3rd generation lentiviral plasmid expressing mCherry

    Depositor

    Pantelis Tsoulfas

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 1, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pSF3-ultraID

    Plasmid

    #172878

    Purpose

    expresses ultraID in mammalian cells with the tet-on or tet-off system

    Depositor

    Julien Béthune

    Article

    Kubitz et al Commun Biol. 2022 Jul 4;5(1):657. doi: 1

    Insert

    ultraID
    

    Use

    Luciferase and Retroviral; To be used in a tet-on…

    Tags

    myc

    Expression

    Mammalian

    Mutation

    fragment ([aa2-171]) of aquifex aeolicus BirA wit…

    Promoter

    TRE-cmv

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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29. pcDNA3-EGFP

    Plasmid

    #13031

    Purpose

    Mammalian expression vector for cloning your gene fused to EGFP

    Depositor

    Doug Golenbock

    Insert

    Enhanced Green Fluorescent Protein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pTT5-hSDC1

    Plasmid

    #52326

    Purpose

    expresses human syndecan 1 in HEK293-EBNA1 (293E) suspension culture.

    Depositor

    Gordon Laurie

    Article

    Zhang et al J Biol Chem. 2013 Apr 26;288(17):12090-1

    Insert

    SDC1 (SDC1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R117Q mutation (R95Q according to numbering in Fi…

    Promoter

    CMV

    Available since

    Dec. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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