169,365 results

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HaloTag-WDR5

Plasmid

#200880

Purpose

Mammalian expression of human WDR5 with N-terminal HaloTag fusion

Depositor

Stefan Knapp

Article

Schwalm et al Cell Chem Biol. 2023 Jun 20:S2451-9456(2

Insert

HaloTag-WDR5 (WDR5 Human)

Expression

Mammalian

Mutation

WT

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pFW2000

Plasmid

#224211

Purpose

E. coli-Bacteroides shuttle plasmid; pB8-51 (Bacteroides replication origin), p15A (E. coli replication origin)

Depositor

Ophelia Venturelli

Article

Feng et al Cell Host Microbe. 2022 Feb 9;30(2):200-

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pC1 Grx1-roCherry-mito

Plasmid

#141069

Purpose

Fluorescent biosensor for 2GSH/GSSG ratio with mitochondrial localization

Depositor

Dmitry Bilan

Article

Shokhina et al Redox Biol. 2019 Feb;21:101071. doi: 10.

Insert

Grx1-roCherry

Tags

dMTS

Expression

Mammalian

Mutation

Changed Alanine 150 to Cysteine, inserted Threoni…

Promoter

CMV

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pSUC01

Plasmid

#177204

Purpose

chromogenic reporter for gene knockout in Streptomyces

Depositor

Wenjun Guan

Article

Dong et al J Microbiol Biotechnol. 2021 Dec 28;31(1

Insert

SaindC

Use

Synthetic Biology

Tags

NONE

Expression

Bacterial

Mutation

NONE

Promoter

TGTTCACATTCGAACGGTCTCTGCTTTGACAACATGCTGTGCGGTGTTG…

Available Since

Jan. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-RIPK3

Plasmid

#78815

Purpose

To overexpress RIPK3 in Mammalian Cells

Depositor

Jaewhan Song

Article

Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

Insert

RIPK3 (RIPK3 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti-hACE2-hygro

Plasmid

#161758

Purpose

Expresses human ACE2 in mammalian cells

Depositor

Neville Sanjana

Article

Daniloski et al Cell. 2020 Oct 24. pii: S0092-8674(20)31

Insert

Human ACE2 (ACE2 Human)

Use

Lentiviral

Tags

P2A

Expression

Mammalian

Promoter

EF1a

Available Since

Nov. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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modRNAc0-ABE8e-P2A-GFP

Plasmid

#178177

Purpose

IVT template plasmid for making ABE8e-P2A-GFP modRNA

Depositor

Xiaojun Lian

Article

Haideri et al Cell Rep Methods. 2022 Sep 7;2(9):100290

Insert

ABE8e

Use

Ivt template plasmid

Tags

P2A-GFP

Promoter

T7 promoter

Available Since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pFP5290 ss-FIREmate-HA- KDEL-IVS-IRES- ss-SBP-mApple- FIREtag-GPI

Plasmid

#216694

Purpose

Synchronize trafficking of GPI-AP from the ER (RUCH system)

Depositor

Franck Perez

Article

Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

Inserts

FIREmate-KDEL
SBP-mApple-FIREtag-GPI

Tags

FIREtag, IL2 signal sequence, Streptavidin Bindin…

Expression

Mammalian

Promoter

CMV

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pCW57.1 _Gata6

Plasmid

#73537

Purpose

Inducible lentiviral expression of Gata6

Depositor

Constance Ciaudo

Article

Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

Insert

Gata6 (Gata6 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE promoter, Tet ON

Available Since

Feb. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pCDH_Str-KDEL_ManII-SBP-EGFP

Plasmid

#65258

Purpose

synchronize trafficking of ManII from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Streptavidin-KDEL and truncated Mannosidase II

Use

Lentiviral

Tags

EGFP and Streptavidin Binding Protein (SBP)

Mutation

contains only amino acids 1 to 116 of ManII

Promoter

CMV

Available Since

Aug. 26, 2015

Availability

Academic Institutions and Nonprofits only

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pBABE puro cyclinD1 HA

Plasmid

#9050

Depositor

William Hahn

Insert

cyclin D1 (CCND1 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Available Since

Nov. 14, 2005

Availability

Academic Institutions and Nonprofits only

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pUSE-Src-YF-UniRapR-mCerulean-myc

Plasmid

#45381

Depositor

Klaus Hahn

Article

Dagliyan et al Proc Natl Acad Sci U S A. 2013 Apr 23;11

Insert

Src-YF-UniRapR-mCerulean-myc

Tags

Myc, Myr, and mCerulean

Expression

Mammalian

Mutation

changed mSrc Tyrosine 529 to Phenylalanine

Promoter

CMV

Available Since

Nov. 7, 2013

Availability

Academic Institutions and Nonprofits only

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pDONR223_KRAS_p.G12V

Plasmid

#81665

Purpose

Gateway Donor vector containing KRAS,part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

KRAS (KRAS Human)

Use

Gateway entry vector

Mutation

G12V

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pSK+KanaRpsL

Plasmid

#20871

Depositor

Jiyue Zhu

Article

Wang et al Mol Biotechnol. 2009 May;42(1):110-6. do

Insert

KanamycinResistance-RpsL

Expression

Bacterial

Mutation

none

Available Since

May 5, 2009

Availability

Academic Institutions and Nonprofits only

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pFW1000

Plasmid

#224210

Purpose

E. coli-Bacteroides shuttle plasmid; pB8-51 (Bacteroides replication origin), R6K (E. coli replication origin)

Depositor

Ophelia Venturelli

Article

Feng et al Cell Host Microbe. 2022 Feb 9;30(2):200-

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pLEX307-TMPRSS2-G418

Plasmid

#158459

Purpose

Constitutive lentiviral expression of human TMPRSS2.

Depositor

Alejandro Chavez, Sho Iketani

Insert

TMPRSS2 (TMPRSS2 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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mStrawberry-N1

Plasmid

#54644

Purpose

Localization: N1 Cloning Vector, Excitation: 574, Emission: 596

Depositor

Michael Davidson, Nathan Shaner, Roger Tsien

Article

Shaner et al Nat Biotechnol. 2004 Dec;22(12):1567-72.

Type

Empty backbone

Tags

mStrawberry

Expression

Mammalian

Available Since

June 11, 2014

Availability

Academic Institutions and Nonprofits only

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pEYFP-Mitotrap

Plasmid

#46942

Purpose

Expresses Mitotrap in mammalian cells

Depositor

Margaret Robinson

Article

Robinson et al Dev Cell. 2010 Feb 16;18(2):324-31. doi:

Insert

Mitotrap (MTOR Aequorea victoria, Synthetic, Human, Budding Yeast)

Tags

This is a fusion between a yeast mitochondrial ta…

Expression

Mammalian

Available Since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only

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pCAGGS SARS-CoV-2 Delta Spike

Plasmid

#185593

Purpose

Expresses codon-optimized full length SARS-CoV-2 Delta Spike

Depositor

Marceline Côté

Article

Benlarbi et al iScience. 2022 Nov 18;25(11):105316. doi

Insert

SARS-CoV-2 Delta Spike (S )

Expression

Mammalian

Mutation

Contains the following mutations: T19R, Δ156-157,…

Promoter

chicken β-actin promoter, CMV enhancer

Available Since

June 8, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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UbC-(mNeonGreen)4-tDeg

Plasmid

#129404

Purpose

(mNeonGreen)4-tDeg fluorogenic protein

Depositor

Samie Jaffrey

Article

Wu et al Nat Methods. 2019 Sep;16(9):862-865. doi

Insert

(mNeonGreen)4-tDeg

Expression

Mammalian

Promoter

UbC

Available Since

Aug. 21, 2019

Availability

Academic Institutions and Nonprofits only

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Nrp2.1-Fc-His

Plasmid

#72098

Purpose

Expresses the extracellular region of the Neuropilin 2, isoform 1 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Nrp2.1 (Nrp2 Mouse)

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only

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CaV1.3e[8a,11,31b,Δ32,42a]

Plasmid

#49333

Purpose

Repaired Addgene #26576 to agree with the rat ref sequence. Repairs are @: nt 3310 (GTG->GCG) [Val->Ala]; nt 731 (TCA->GGA) [Ser->Gly].

Depositor

Diane Lipscombe

Article

Xu et al J Neurosci. 2001 Aug 15. 21(16):5944-51.

Insert

CACNA1D (Cacna1d Rat)

Expression

Mammalian

Mutation

Contains exons 8a, 11, 31b and 42a.Deletion of ex…

Promoter

CMV

Available Since

Feb. 14, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3-luxNeon

Plasmid

#229947

Purpose

Mammalian expression of a de novo designed, FRET-based neoLux1.2-mNeonGreen fusion bioluminescent probe (max. Em=518nm)

Depositor

Andy Yeh

Article

Chen et al Chem. 2025 Mar 13;11(3):102346. doi: 10.

Insert

luxNeon

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 2, 2025

Availability

Academic Institutions and Nonprofits only

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TFORF1920

Plasmid

#142887

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

RUNX1 (RUNX1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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TFORF1509

Plasmid

#143511

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

STAT5B (STAT5B Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

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pGGB_YFP-MiniTurboID

Plasmid

#222433

Purpose

Golden Gate / Green Gate module for adding YFP and MiniTurboID as N-terminus of target protein.

Depositor

Dominique Bergmann

Article

Wallner et al Dev Cell. 2024 May 6;59(9):1096-1109.e5.

Insert

MiniTurboID (BirA mutant)

Use

Golden gate / green gate compatible cloning vector

Tags

Linker and YFP

Mutation

aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

Available Since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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mCherry-INPP5K

Plasmid

#171950

Purpose

Expresses INPP5K in mammalian cells

Depositor

Cheng-han Yu

Article

Feng et al Proc Natl Acad Sci U S A. 2021 May 11;11

Insert

INPP5K (INPP5K Human)

Tags

mCherry

Expression

Mammalian

Available Since

July 13, 2021

Availability

Academic Institutions and Nonprofits only

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pTLR repair vector

Plasmid

#64322

Purpose

Vector for repair of the traffic light reporter (TLR), providing the venus coding region

Depositor

Ralf Kuehn

Article

Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

Insert

Venus

Use

Artificial targeting vector

Mutation

see publication for details

Promoter

none

Available Since

May 19, 2015

Availability

Academic Institutions and Nonprofits only

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IMS-HyPer2

Plasmid

#60248

Purpose

Genetically-encoded fluorescent hydrogen peroxide indicator HyPer2 targeted to mitochondrial intermembrane spase.

Depositor

Vsevolod Belousov

Article

Ermakova et al Nat Commun. 2014 Oct 21;5:5222. doi: 10.

Insert

IMS-HyPer2

Tags

Mitochondrial intermembrane space targeting signa…

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pKIKOlacZKm

Plasmid

#46767

Purpose

E.coli genomic integration at lacZ

Depositor

Lars Nielsen, Claudia Vickers

Article

Sabri et al Microb Cell Fact. 2013 Jun 24;12(1):60.

Type

Empty backbone

Use

E.coli genomic integration

Available Since

Sept. 24, 2013

Availability

Academic Institutions and Nonprofits only

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