171,784 results
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SBE-NLS-mCherry-P2A-CreERT2 PGK-rtTA3
Plasmid#196936PurposeTGFbeta reporter driven nuclear mCherry and CreERT2 with PGK driven rtTA3DepositorInsertmCherry
UseLentiviral and RNAiTagsP2A-CreERT2ExpressionMammalianPromoterSBEAvailable SinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJR89
Plasmid#140096PurposesgRNA constant region and hU6 insert for programmed dual sgRNA cloning. The sgRNA constant region contains a capture sequence (cs1) in the stem loop for direct capture Perturb-seq.DepositorInsertsgRNA constant region CR3 with cs1 in stem loop and hU6 promoter
Available SinceJuly 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
TFORF2281
Plasmid#142929PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable SinceJune 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/NDUFV2/myc-His
Plasmid#127491PurposeExpresses myc-tagged NDUFV2 in mammalian cellsDepositorAvailable SinceJuly 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-C1 R62D actin-3XNLS P2A mCherry
Plasmid#58477PurposeExpresses nuclear-targeted non-polymerizing R62D mutant of human actin, with an mCherry expression reporter after a P2A protease cleavage site, on a CMV promoterDepositorInsertsTagsmCherryExpressionMammalianMutationChanged Arginine 62 to Aspartic AcidPromoterCMVAvailable SinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
HA-HIF2alpha-P405A/P531A-pcDNA3
Plasmid#18956DepositorInsertHypoxia inducible factor 2 alpha (EPAS1 Human)
TagsHA-tagExpressionMammalianMutationcDNA changed amino acids: Proline 405 to Alanin…Available SinceAug. 20, 2008AvailabilityAcademic Institutions and Nonprofits only -
FLAG-HA-pcDNA3.1-
Plasmid#52535Purposeenables eukaryotic expression of N-terminally FLAG-HA-tagged fusion proteinsDepositorHas ServiceCloning Grade DNATypeEmpty backboneTagsFLAG-HAExpressionMammalianPromoterCMVAvailable SinceJuly 31, 2015AvailabilityAcademic Institutions and Nonprofits only -
Accessory plasmid 2
Plasmid#128571Purposefor cloning identified individual SPECSDepositorTypeEmpty backboneUseLentiviralTagsTagRFPAvailable SinceJuly 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pSOUP
Plasmid#165419PurposeThe pSOUP helper plasmid needed for replication of all pGreen (Hellens et al., 2000) based vectors in AgrobacteriumInsertRep A
UseHelper plasmid required for replication for all p…Available SinceApril 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUC19_HDRT_TRAC_CD19-CAR
Plasmid#183473PurposeEncodes for a homology-directed DNA repair template for the insertion of a second generation CD19-specific CAR into the TRAC geneDepositorAvailable SinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pmr101A-cPr-mCherry (KmR)
Plasmid#177207PurposePlasmid with p15A origin for constitutive mCherry expression in E. coliDepositorInsertmCherry
ExpressionBacterialPromoterSynthetic constitutive promoterAvailable SinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAJE3-E7
Plasmid#214359PurposeExpresses the 2nd-generation "E7" Tet2 Methanocaldococcus jannaschii (Mj) aminoacyl tRNA synthetase/tRNA pair for encoding Tet2 noncanonical amino acids into TAG sites of proteinsDepositorInserts2nd generation Tet2 "E7" aminoacyl tRNA synthetase
Lpp promoted M. jannaschii tRNA
Aminoglycoside-3''-adenyltransferase (Spectinomycin/streptomycin Resistance Gene)
Orthogonal "D4II" ColE1 origin
ExpressionBacterialPromoterAmpR and lppAvailable SinceMarch 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
P4-PE
Plasmid#211375PurposePackaging plasmid for v3b PE-eVLP productionDepositorInsertP4-PE
ExpressionMammalianAvailable SinceJan. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV phSyn1(S)-FLEX-EGFP-WPRE
Plasmid#51504PurposeCan be used to generate AAV virus that will express EGFP in the presence of Cre in neurons from the synapsin promoterDepositorInsertEGFP
UseAAVPromoterphSyn1Available SinceMay 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pFE-Reu-FI-prk
Plasmid#162696PurposeExpresses R. eutropha Form IC rubisco and S. elongatus PrkDepositorInsertR. eutropha CbbLS and S. elongatus Prk
TagsN terminal 6x His tag on PRKExpressionBacterialPromoterPLtet0-1 promoterAvailable SinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNIR-FbLAG30
Plasmid#220740PurposeNIR-FbLAG30 expression in mammalian cells.DepositorInsertNIR-FbLAG30
UseAffinity Reagent/ AntibodyExpressionMammalianPromoterCMVAvailable SinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
B*58:01:01
Plasmid#203261PurposeOverexpress HLA alleleDepositorInsertB*58:01:01 (HLA-B Human)
ExpressionMammalianAvailable SinceJuly 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF2432
Plasmid#144026PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable SinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
GFP-Rab5DN(S34N)
Plasmid#35141DepositorInsertRab5a (RAB5A Human)
TagsEGFPExpressionMammalianMutationDominant Negative- S34NPromoterCMVAvailable SinceMarch 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS DRR mCherry Donor EF1a BFP
Plasmid#98896PurposeHomologous recombination donor for pDRR. Expresses mCherry after repait by HR.DepositorInsertNeomycin-T2A-mCherry-GFP
UseSynthetic BiologyExpressionMammalianAvailable SinceNov. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
TRE-UBC-mCD47-T2A-GFP
Plasmid#205449Purposelentiviral plasmid for expression of mouse CD47DepositorAvailable SinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCS2-mCherry_SNAP_SNAPV_PACT_Active
Plasmid#173072PurposeActive SNAP tag at the centrosomeDepositorInsertmCherry-SNAP-SNAP-Varied-PACT
TagsmCherry-SNAP-SNAP-VariedExpressionMammalianPromoterCMV IE94Available SinceAug. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_ERN1_WT
Plasmid#82115PurposeGateway Donor vector containing ERN1 , part of the Target Accelerator Plasmid Collection.DepositorAvailable SinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV_NESPylRS(AF)_hU6tRNAPyl
Plasmid#223508PurposePylRS (AF)/tRNA Pyl orthogonal pair for genetic code expansion that allows site-specific incorporation of noncanonical amino-acids into a POI using the Amber stop codonDepositorInsertsExpressionMammalianMutationY306A; Y384FPromoterCVM, SP6 and U6Available SinceOct. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti HsATP13A2 WT
Plasmid#171485Purposetransfer plasmid for lentiviral vector production expressing Hs ATP13A2 WTDepositorAvailable SinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-E2-Crimson
Plasmid#197196PurposeExpresses the protein of E2-Crimson in mammalian cellsDepositorInsertE2-Crimson
UseAAVAvailable SinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
6 x DBE-luc
Plasmid#153160PurposeLuciferase reporter plasmid for FOXTFDepositorInsertluciferase
UseLuciferasePromoterminPAvailable SinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
TFORF2061
Plasmid#141900PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable SinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-PC-GCaMP6f
Plasmid#73503PurposeGCaMP6f knock in construct into the AAVS1 locusDepositorInsertGCaMP6f
ExpressionMammalianPromoterCAGAvailable SinceDec. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
GFP HDAC5 WT
Plasmid#32211DepositorAvailable SinceNov. 2, 2011AvailabilityAcademic Institutions and Nonprofits only -
pTXB1-nbMmIgG1-Tn5
Plasmid#184287PurposeAnti-mouse IgG1 Fc specific nanobody-Tn5 for NTT-seq (multiplexed CUT&Tag)DepositorInsertnbMmIgG1-Tn5
Available SinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLminP_Luc2P_RE10
Plasmid#90344PurposeIRF3 - Viral response gene reporterDepositorInsertFirefly luciferase
UseLentiviralPromoterIRF3Available SinceAug. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDL278_P23-DsRed-Express2
Plasmid#121505PurposeE. coli-streptococcal shuttle vector that expresses rfpDepositorInsertDsRed-Express2
UseE. coli streptococcal shuttle vectorPromoterP23Available SinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_SHC1_WT_V5
Plasmid#82931PurposeGateway Donor vector containing SHC1, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.DepositorAvailable SinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
TMEM63A-W472K-peGFP-N1
Plasmid#228591PurposeExpresses mouse TMEM63A-W472K-eGFP in mammalian cellsDepositorAvailable SinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
TFORF0442
Plasmid#143026PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable SinceFeb. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
GFP-p38beta
Plasmid#86833Purposemammalian expression of GFP-p38betaDepositorAvailable SinceFeb. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-survivin
Plasmid#196908Purposevisualization of midzone on anaphase and midbody later during mitosis in living mammalian cellsDepositorInsertsurvivin
TagsEGFPExpressionMammalianMutationE129KAvailable SinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET30a-His6-LOXCATmut
Plasmid#140085PurposeA control for His6-LOXCAT where LOX is catalytically dead (mutations H265A and R268A)DepositorInsertA fusion of lactate oxidase from A. viridans and catalase from E. coli
TagsHis6ExpressionBacterialMutationMutation in H265A and R268A in LOX (used original…PromoterT7Available SinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCC_09 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-KRAB-dCas9-NLS-2A-Puro-WPRE
Plasmid#139094PurposeExpresses human codon-optimized inactive SpCas9 fused to a transcriptional repressor KRAB in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.DepositorInsertKRAB-dSpCas9
UseCRISPR and LentiviralExpressionMammalianMutationD10A, H840AAvailable SinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRSV-CaMKII(1-290)
Plasmid#45065PurposeExpression of constit active CaMKIIDepositorInsertCaM Kinase II (Camk2a Rat)
ExpressionMammalianMutationtruncated at amino acid 290PromoterRSVAvailable SinceJuly 31, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET30a-His6-ABP-LOXCAT
Plasmid#139971PurposeFusion of A. viridans lactate oxidase (LOX) and E. coli catalase (CAT) that converts lactate into pyruvate without producing H2O2. Contains a albumin binding peptide (ABP).DepositorInsertA fusion of lactate oxidase from A. viridans and catalase from E. coli
TagsHis6ExpressionBacterialPromoterT7Available SinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcpBpaRS_v2
Plasmid#196485PurposeTo express benzoylphenylalanine-specific variant of E. coli TyrRS (pBpaRS) and its cognate amber suppressor tRNA to allow UAG codon to be translated into benzoylphenylalanine.DepositorInsertbenzoylphenylalanine-specific variant of E. coli TyrRS (pBpaRS), amber suppressor tRNA
ExpressionMammalianPromoterSV40(synthetase), Pol III tRNA promoter (tRNA), C…Available SinceMarch 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCE-hOCT3/4
Plasmid#41813PurposeNon-integrating (episomal) expression of human OCT3/4DepositorAvailable SinceApril 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
HA-POMP
Plasmid#86765PurposeN-terminal HA-tagged protein expression in mammalian cellsDepositorAvailable SinceJan. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
hDAGLa-V5
Plasmid#87676PurposeExpresses human DAGL? protein with intracellular V5 tag located on the C-terminalDepositorAvailable SinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFastBac TwinStrepII Prescission SNAPf TEV cloning vector with BioBrick PolyPromoter LIC Subcloning (438-SNAP-V1)
Plasmid#55222DepositorTypeEmpty backboneTagsSNAPf and TwinStrepIIExpressionInsectAvailable SinceJune 17, 2014AvailabilityAcademic Institutions and Nonprofits only
