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159,134 results

Showing: 4951 - 5000 of 159134 results

  1. pCL28-decoder

    Plasmid
    #159450
    Purpose
    Insert: PlexAtet-lexA-ER-B112-tCyc1. The transcription factor LexA-ER-B112 (see addgene FRP880) is self-inducible upon addition of estradiol, and repressible by TetR. Backbone: pRG206MX. Marker: Ura3.
    Depositor
    Joerg Stelling
    Article
    Lormeau et al Nat Commun. 2021 Mar 25;12(1):1886. doi:
    Insert
    PlexAtet-LexA-ER-B112-tCyc1
    Use
    Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    PlexAtet
    Available since
    Dec. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    159450-plasmid-map-sequence-id-313198

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  2. pCL66-decoder

    Plasmid
    #159453
    Purpose
    Insert: Pfus1mut-tetR-nls-malE-tCyc1. Same insert as pCL33-decoder in a multiple integration shuttle vector. Backbone: pRG235 (addgene). Marker: Leu2.
    Depositor
    Joerg Stelling
    Article
    Lormeau et al Nat Commun. 2021 Mar 25;12(1):1886. doi:
    Insert
    Pfus1mut-tetR-nls-malE-tCyc1
    Use
    Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Pfus1mut
    Available since
    Dec. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    159453-plasmid-map-sequence-id-313208

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  4. pMT646 human L1 ORF2p-3xFlag (ORFeus-Hs, CMV promoter) in pCEP4 Puro

    Plasmid
    #213026
    Purpose
    Expresses full length human LINE-1 in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2
    Depositor
    Martin S. Taylor
    Article
    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:
    Insert
    Human LINE-1 (ORFeus-Hs codon optimized sequence) with ORF2-3xFlag
    Use
    Tags
    3xFlag (ORF2p)
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    May 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    213026-plasmid-map-sequence-id-422069

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  6. pExp-GST

    Plasmid
    #112566
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and GST fusions with optional C-terminal StrepII-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Use
    Tags
    8xHis-GST and Strep tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    112566-plasmid-map-sequence-id-217229

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  20. pExp-NusA

    Plasmid
    #112571
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and NusA fusions with optional C-terminal StrepII-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Use
    Tags
    8His and Strep tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    112571-plasmid-map-sequence-id-220666

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  21. phCMV-coGALVenv-C4070A

    Plasmid
    #172217
    Purpose
    Expresses the envelope protein of Gibbon Ape Leukemia Virus (GALV) for pseudotyping of retroviral or lentiviral vectors
    Depositor
    Boris Fehse
    Article
    Mirow et al Viruses. 2021 Jul 27;13(8). pii: v130814
    Insert
    Envelope protein of Gibbon Ape Leukemia Virus (codon-optimized version)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Carries C-terminus of amphotropic MLV strain 4070…
    Promoter
    CMV immediate early promoter
    Available since
    Aug. 31, 2021
    Availability
    Academic Institutions and Nonprofits only
    172217-plasmid-map-sequence-id-341502

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  22. pHIV-Luc-ZsGreen

    Plasmid
    #39196
    Depositor
    Bryan Welm
    Inserts
    Firefly Luciferase Luc2P
    ZsGreen
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1-alpha and EF1-alpha
    Available since
    Oct. 30, 2012
    Availability
    Academic Institutions and Nonprofits only
    39196-plasmid-map-sequence-id-335121

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  23. pAcHELIX-sgRNA_entry (CJT94)

    Plasmid
    #181783
    Purpose
    Expresses AcHELIX containing a nicking I-AniI fusion to AcTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).
    Depositor
    Benjamin Kleinstiver
    Article
    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    nAniI-AcTnsB, AcTnsC, AcTniQ, AcCas12k
    Use
    Tags
    Expression
    Bacterial
    Mutation
    nAniI = K227M, F80K, L232K
    Promoter
    Lac and J23119
    Available since
    March 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    181783-plasmid-map-sequence-id-358223

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  25. Lyso-B

    Plasmid
    #209873
    Purpose
    Dimerization dependent fluorescent protein B anchored to cytosolic face of the lysosomal membrane with human LAMP1
    Depositor
    Sarah Cohen
    Article
    Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25
    Insert
    ddGFP B
    Use
    Tags
    LAMP1
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    209873-plasmid-map-sequence-id-415450

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  32. TFORF0074

    Plasmid
    #142144
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    BACH2 (BACH2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    142144-plasmid-map-sequence-id-397722

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  43. pAAV-hSyn-DIO {ChETA-mRuby2}on-W3SL

    Plasmid
    #111389
    Purpose
    AAV vector with hSynapsin promoter, Cre-ON ChETA-mRuby2 (for optogenetic activation), and W3SL regulatory cassette (for maximize cloning capacity)
    Depositor
    Adam Kepecs
    Article
    Li et al Neuron. 2018 Jun 6;98(5):905-917.e5. doi
    Inserts
    ChETA-mRuby2
    W3SL
    Use
    AAV
    Tags
    mRuby2
    Expression
    Mammalian
    Mutation
    W3SL is built from a shortened WPRE, an upstream …
    Promoter
    hSynapsin
    Available since
    June 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    111389-plasmid-map-sequence-id-212217

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  45. PX459v3

    Plasmid
    #178799
    Purpose
    SpCas9 with 2A-Puro, and a golden gate cloning backbone for sgRNA. sgRNA scaffold seqeuence has been modified for increased sgRNA expression (v3).
    Depositor
    Paul Thomas
    Article
    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Cbh (Cas9-2A Puro) and U6 (gRNA)
    Available since
    Aug. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    178799-plasmid-map-sequence-id-354842

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  49. TFORF0282

    Plasmid
    #141561
    Purpose
    Lentiviral vector for overexpressing the MEF2C transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    MEF2C (MEF2C Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    141561-plasmid-map-sequence-id-276819

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Showing: 4951 - 5000 of 159134 results