4,075 results

1. CMV-G-GECO1.1

   Plasmid

   #32445

   Purpose

   Mammalian expression of green fluorescent genetically encoded Ca2+ indicator for optical imaging (version 1.1)

   Depositor

   Robert Campbell

   Article

   Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

   Insert

   G-GECO1.1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   GCaMP3 K69E/N77Y/D86G/K119I/L173Q/K380N/S404G/E43…

   Promoter

   CMV

   Available since

   Sept. 29, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pENTR/pSUPER+ (575-1)

   Plasmid

   #17338

   Purpose

   Gateway entry vector for shRNA expression under H1 promoter

   Depositor

   Paul Kaufman, Eric Campeau

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Type

   Empty backbone

   Use

   RNAi; Entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. V51 pIRESpuro-GLUE (pGLUE)

   Plasmid

   #15100

   Purpose

   tandem-affinity purification plasmid; streptavidin-(streptag) and calmodulin-(CBP) binding affinity tags

   Depositor

   Randall Moon

   Article

   Angers et al Nat Cell Biol. 2006 Apr . 8(4):348-57.

   Type

   Empty backbone

   Use

   Tags

   CBP tag, HA, and streptag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 28, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBABE-neo largeTgenomic

   Plasmid

   #10891

   Depositor

   William Hahn

   Insert

   large T and small T antigen (SV40gp6 simian virus)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 7, 2005

   Availability

   Academic Institutions and Nonprofits only

5. pRK5-myc-Rac1-T17N

   Plasmid

   #12984

   Depositor

   Gary Bokoch

   Insert

   Rac1 DN (RAC1 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   T17N Dominant negative

   Promoter

   Available since

   Oct. 27, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. FUW-tetO-lox-hOCT4

   Plasmid

   #20728

   Depositor

   Rudolf Jaenisch

   Article

   Soldner et al Cell. 2009 Mar 6. 136(5):964-77.

   Insert

   OCT4 (POU5F1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pTiger

   Plasmid

   #1728

   Purpose

   This is a 3rd generation lentiviral transfer vector (highest titer) from Nolan lab's FELIX vector system

   Depositor

   Garry Nolan

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 18, 2005

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. MSP1101

   Plasmid

   #65773

   Purpose

   Human expression vector for SpCas9 VRER variant: CMV-T7-humanSpCas9(D1135V/G1218R/R1335E/T1337R)-NLS-3xFLAG (VRER variant)

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

   Insert

   mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135V/G1218R/R1335E/T1337R)-NLS-3XFlag
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Mammalian

   Mutation

   D1135V, G1218R, R1335E, and T1337R mutations in C…

   Promoter

   CMV & T7

   Available since

   June 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. piRFP702-N1

   Plasmid

   #45456

   Depositor

   Vladislav Verkhusha

   Article

   Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

   Insert

   iRFP702
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   T2A, S45T, D124E, M170L, D202L, I203V, I219V, R23…

   Promoter

   CMV

   Available since

   June 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSV Sport PPAR gamma 2

    Plasmid

    #8862

    Depositor

    Bruce Spiegelman

    Article

    Tontonoz et al Genes Dev. 1994 May 15. 8(10):1224-34.

    Insert

    PPAR gamma (Pparg Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. FCK-Arch-GFP

    Plasmid

    #22217

    Purpose

    Lentiviral expression of Arch-GFP for optogenetic inhibition

    Depositor

    Edward Boyden

    Article

    Chow et al Nature. 2010 Jan 7. 463(7277):98-102.

    Insert

    Arch
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CaMKIIa

    Available since

    Jan. 6, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. MSP2133

    Plasmid

    #72249

    Purpose

    Human expression plasmid for SpCas9-HF4 variant: CMV-T7-humanSpCas9-HF4(Y450A, N497A, R661A, Q695A, Q926A)-NLS-3xFLAG

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1

    Insert

    mammalian codon-optimized Streptococcus pyogenes Cas9 HF4(Y450A/N497A/R661A/Q695A/Q926A)-NLS-3xFlag
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Mammalian

    Mutation

    Y450A, N497A, R661A, Q695A, and Q926A

    Promoter

    CMV

    Available since

    Jan. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. 1013 pcDNA3 T7 Akt1 T308A S473A

    Plasmid

    #9030

    Depositor

    William Sellers

    Article

    Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

    Insert

    Akt1 T308A S473A (AKT1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    T308A S473A

    Promoter

    Available since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. TetR in pENTR1A (559-1)

    Plasmid

    #22265

    Depositor

    Eric Campeau

    Insert

    Tetracycline repressor (tetR )
    

    Use

    Tags

    Expression

    Insect, Mammalian, Worm Exp…

    Mutation

    Promoter

    Available since

    Dec. 17, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCMV5 mouse src K295R Y527F

    Plasmid

    #13657

    Depositor

    Joan Brugge, Peter Howley

    Insert

    src K295R Y527F (Src Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    K295R Y527F. Dominant negative mutant.

    Promoter

    Available since

    Jan. 4, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA-HA-Nedd4/2795

    Plasmid

    #11426

    Depositor

    Allan Weissman

    Article

    Magnifico et al J Biol Chem. 2003 Oct 31. 278(44):43169-

    Insert

    Nedd4-1 (Nedd4 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    D661N compared to GenBank reference sequence U966…

    Promoter

    CMV

    Available since

    April 20, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. PGKneolox2DTA.2

    Plasmid

    #13449

    Depositor

    Philippe Soriano

    Insert

    PGKneolox2DTA
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3-CaMPARI V398D

    Plasmid

    #60786

    Purpose

    Expresses CaMPARI V398D in mammalian cells, a photoconvertible fluorescent protein-based calcium integrator

    Depositor

    Eric Schreiter, Loren Looger

    Article

    Fosque et al Science. 2015 Feb 13;347(6223):755-60. d

    Insert

    CaMPARI V398D
    

    Use

    Tags

    Nuclear Export Signal

    Expression

    Mammalian

    Mutation

    V398D

    Promoter

    CMV

    Available since

    Nov. 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTriEx-RhoA FLARE.sc Biosensor T19N

    Plasmid

    #12152

    Depositor

    Klaus Hahn

    Article

    Pertz et al Nature. 2006 Apr 20. 440(7087):1069-72.

    Insert

    RhoA Biosensor T19N (RHOA Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    RhoA T19N (dominant-negative mutation)*

    Promoter

    Available since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. CAG-mBrn2

    Plasmid

    #19711

    Depositor

    Connie Cepko

    Article

    Kim et al J Neurosci. 2008 Jul 30. 28(31):7748-64.

    Insert

    mouse Brn2 (Pou3f2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 27, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. Clathrin-LCa-EYFP

    Plasmid

    #21741

    Depositor

    Xiaowei Zhuang

    Article

    Chen et al Proc Natl Acad Sci U S A. 2008 Aug 19. 1

    Insert

    Clathrin, light polypeptide (LCa) (Clta Mouse)
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 5, 2009

    Availability

    Academic Institutions and Nonprofits only

22. pLM-mCitrine-Sox2

    Plasmid

    #23242

    Depositor

    Michel Sadelain

    Article

    Papapetrou et al Proc Natl Acad Sci U S A. 2009 Aug 4. 10

    Insert

    mCitrine_2A_SOX2 (SOX2 synthetic fluorescent protein, Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. MSCV Myc IRES GFP

    Plasmid

    #18770

    Depositor

    Scott Lowe

    Article

    Hemann et al Nature. 2005 Aug 11. 436(7052):807-11.

    Insert

    Myc (Myc Mouse)
    

    Use

    Retroviral

    Tags

    IRES GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 3, 2008

    Availability

    Academic Institutions and Nonprofits only

24. 1271 pBabe puroL Myr HA Akt2

    Plasmid

    #9018

    Depositor

    William Sellers

    Insert

    Akt2 (AKT2 Human)
    

    Use

    Retroviral

    Tags

    HA and Myr

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 2, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pSV Sport PPAR gamma 1

    Plasmid

    #8886

    Depositor

    Bruce Spiegelman

    Article

    Tontonoz et al Genes Dev. 1994 May 15. 8(10):1224-34.

    Insert

    PPAR gamma (Pparg Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

26. pDD283

    Plasmid

    #66824

    Purpose

    YPET^SEC^3xFlag vector with ccdB markers for cloning homology arms

    Depositor

    Bob Goldstein

    Article

    Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.

    Insert

    YPET-C1^SEC^3xFlag
    

    Use

    CRISPR and Cre/Lox

    Tags

    3xFlag and C. elegans codon-optimized YPET

    Expression

    Worm

    Mutation

    Promoter

    Available since

    June 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. TetO-FUW-Klf4

    Plasmid

    #20322

    Depositor

    Rudolf Jaenisch

    Article

    Brambrink et al Cell Stem Cell. 2008 Feb 7. 2(2):151-9.

    Insert

    Klf4 (Klf4 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pGEM-Oct4

    Plasmid

    #16352

    Depositor

    James Thomson

    Article

    Yu et al Science. 2007 Dec 21. 318(5858):1917-20.

    Insert

    Homo sapiens POU domain, class 5, transcription factor 1 (POU5F1) (POU5F1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 4, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. siSlug3

    Plasmid

    #10905

    Depositor

    Bob Weinberg

    Article

    Gupta et al Nat Genet. 2005 Oct . 37(10):1047-54.

    Insert

    siSlug (SNAI2 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCEP4CyPet-MAMM

    Plasmid

    #14033

    Depositor

    Patrick Daugherty

    Article

    Nguyen et al Nat Biotechnol. 2005 Mar . 23(3):355-60.

    Insert

    CyPet
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Mammalian optimized CyPet

    Promoter

    Available since

    Feb. 14, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pShuttle-Cre-HA

    Plasmid

    #16583

    Depositor

    Bert Vogelstein

    Article

    Jallepalli et al Cell. 2001 May 18. 105(4):445-57.

    Insert

    cre recombinase (cre P1 bacteriophage)
    

    Use

    Adenoviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 12, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. shp63alpha pLKO.1 puro

    Plasmid

    #19120

    Depositor

    Bob Weinberg

    Article

    Godar et al Cell. 2008 Jul 11. 134(1):62-73.

    Insert

    small hairpin RNA against tumor protein p63 (TP63 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 9, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pMKO.1 puro p53 shRNA 2

    Plasmid

    #10672

    Depositor

    William Hahn

    Article

    Masutomi et al Cell. 2003 Jul 25. 114(2):241-53.

    Insert

    p53 shRNA (TP53 Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 6, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. DsRed-rab9 DN

    Plasmid

    #12676

    Depositor

    Richard Pagano

    Article

    Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

    Insert

    RAB9 (Homo sapiens) (RAB9A Human)
    

    Use

    Tags

    DsRed and HA

    Expression

    Mammalian

    Mutation

    changed serine 21 to asparagine

    Promoter

    Available since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pMSCV-TAP

    Plasmid

    #12570

    Depositor

    David Sabatini

    Article

    Frias et al Curr Biol. 2006 Aug 15. ():.

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    TAP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 1, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pcDNA/Myc DeltaN TCF4

    Plasmid

    #16513

    Depositor

    Bert Vogelstein

    Article

    Korinek et al Science. 1997 Mar 21. 275(5307):1784-7.

    Insert

    TCF4 (TCF7L2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Delta N: lacks aa 1-32

    Promoter

    Available since

    May 16, 2008

    Availability

    Academic Institutions and Nonprofits only

37. pRosa26PAm1 (DM#272)

    Plasmid

    #15036

    Depositor

    Douglas Melton

    Article

    Murtaugh et al Proc Natl Acad Sci U S A. 2003 Dec 9. 10

    Insert

    pRosa26 modified targeting vector
    

    Use

    For making transgenic constructs

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. M-NMn-VP64

    Plasmid

    #48676

    Purpose

    Mammalian NM-VP64 nuclease-null Cas9 activator expression, human optimized

    Depositor

    George Church

    Article

    Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

    Insert

    Cas9-VP64, nuclease-null
    

    Use

    CRISPR

    Tags

    NLS and VP64

    Expression

    Mutation

    nuclease-null

    Promoter

    CMV

    Available since

    Oct. 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pAAV-GFAP-hChR2(H134R)-mCherry

    Plasmid

    #27055

    Purpose

    AAV expression of humanized ChR2(H134R) fused to mCherry, for optogenetic excitation

    Depositor

    Karl Deisseroth

    Insert

    hChR2(H134R)
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    H134R

    Promoter

    GFAP

    Available since

    Jan. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCMV5 Smad2-HA

    Plasmid

    #14930

    Depositor

    Joan Massague

    Article

    Hata et al Nature. 1997 Jul 3. 388(6637):82-7.

    Insert

    Smad2 (SMAD2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 17, 2007

    Availability

    Academic Institutions and Nonprofits only