4,133 results

1. pLVX-EF1alpha-SARS-CoV-2-orf8-2xStrep-IRES-Puro

   Plasmid

   #141390

   Purpose

   Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

   Depositor

   Nevan Krogan

   Article

   Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

   Insert

   SARS-CoV-2-orf8 (ORF8 SARS-CoV-2)
   

   Use

   Lentiviral

   Tags

   2xStrep

   Expression

   Mammalian

   Mutation

   human codon optimized

   Promoter

   EF1alpha

   Available since

   April 8, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. pAct-Cas9

   Plasmid

   #62209

   Purpose

   Expresses Cas9 under control of act5C promoter and SV40 3'UTR. For ubiquitous genome engineering in Drosophila melanogaster.

   Depositor

   Simon Bullock

   Article

   Port et al Proc Natl Acad Sci U S A. 2014 Jul 7. pi

   Insert

   cas9
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   act5C

   Available since

   March 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pPAGFP-C1

   Plasmid

   #11910

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Patterson et al Science. 2002 Sep 13. 297(5588):1873-7.

   Has Service

   Cloning Grade DNA

   Insert

   PAGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   These plasmids are essentially the same as the Cl…

   Promoter

   Available since

   July 3, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. xCas9(3.7)-BE3

   Plasmid

   #108380

   Purpose

   Mammalian expression vector for xCas9(3.7)-BE3

   Depositor

   David Liu

   Article

   Hu et al Nature. 2018 Feb 28. pii: nature26155. d

   Insert

   xCas9(3.7)-BE3
   

   Use

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   A262T, R324L, S409I, E480K, E543D, M694I, E1219V

   Promoter

   Available since

   March 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. MSCV H-Ras V12 IRES GFP

   Plasmid

   #18780

   Depositor

   Scott Lowe

   Insert

   H-Ras V12 (HRAS Human)
   

   Use

   Retroviral

   Tags

   IRES GFP

   Expression

   Mammalian

   Mutation

   V12

   Promoter

   Available since

   July 3, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCMV4-BlaM-Vpr

   Plasmid

   #21950

   Depositor

   Warner Greene

   Article

   Cavrois et al Nat Biotechnol. 2002 Nov . 20(11):1151-4

   Insert

   BlaM-Vpr (vpr Fusion of BlaM and HIV-1 Vpr)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 1, 2009

   Availability

   Academic Institutions and Nonprofits only

7. pCMV-HA-JMJD2B

   Plasmid

   #24181

   Purpose

   Mammalian expression of human JMJD2B with HA tag

   Depositor

   Kristian Helin

   Article

   Cloos et al Nature. 2006 Jul 20. 442(7100):307-11.

   Insert

   JMJD2B (KDM4B Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 24, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pEGFP-N1-delta30-TFEB

   Plasmid

   #44445

   Depositor

   Shawn Ferguson

   Article

   Roczniak-Ferguson et al Sci Signal. 2012 Jun 12;5(228):ra42.

   Insert

   TFEB (TFEB Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Deleted amino acids 1-30; H32D

   Promoter

   Available since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMA122 - peel-1 negative selection

   Plasmid

   #34873

   Purpose

   Negative selection marker for use with the Mos-1 mediated system of creating targeted transgene insertions and targeted deletions in C. elegans

   Depositor

   Erik Jorgensen

   Article

   Frokjaer-Jensen et al Nat Methods. 2012 Jan 30;9(2):117-8. doi

   Insert

   peel-1 (peel-1 Nematode)
   

   Use

   Worm targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 1, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMAL-his-LbCpf1-EC

    Plasmid

    #79008

    Purpose

    Bacterial expression - MBP-his tagged LbCpf1 (E.coli codon optimized)

    Depositor

    Jin-Soo Kim

    Article

    Kim et al Nat Biotechnol. 2016 Jun 6. doi: 10.1038

    Insert

    LbCpf1
    

    Use

    Tags

    MBP-6xHis-NLS

    Expression

    Bacterial

    Mutation

    Promoter

    tac promoter

    Available since

    July 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCI-SEP_NR2B

    Plasmid

    #23998

    Depositor

    Robert Malinow

    Article

    Kopec et al J Neurosci. 2006 Feb 15. 26(7):2000-9.

    Insert

    NR2B (Grin2b Rat)
    

    Use

    Tags

    NR2B signal sequence (aa1-31) and SEP (supereclip…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. FUW

    Plasmid

    #14882

    Depositor

    David Baltimore

    Article

    Lois et al Science. 2002 Feb 1. 295(5556):868-72.

    Insert

    flap-Ub promoter-WRE
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 25, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMLS-SV40-EGFP

    Plasmid

    #46919

    Purpose

    Target EGFP gene that is stably integrated into HEK293 genome

    Depositor

    Stanley Qi, Jonathan Weissman

    Article

    Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

    Insert

    GFP
    

    Use

    CRISPR and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    Oct. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. mApple-C1

    Plasmid

    #54631

    Purpose

    Localization: C1 Cloning Vector, Excitation: 568, Emission: 592

    Depositor

    Michael Davidson

    Article

    Kremers et al Nat Methods. 2009 May;6(5):355-8. doi: 1

    Type

    Empty backbone

    Use

    Tags

    mApple

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAAV-GFAP-hChR2(H134R)-mCherry

    Plasmid

    #27055

    Purpose

    AAV expression of humanized ChR2(H134R) fused to mCherry, for optogenetic excitation

    Depositor

    Karl Deisseroth

    Insert

    hChR2(H134R)
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    H134R

    Promoter

    GFAP

    Available since

    Jan. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3.1(+) ZKSCAN1 MCS Exon Vector

    Plasmid

    #69901

    Purpose

    Expression plasmid for expressing circular RNAs of a desired sequence in mammalian cells

    Depositor

    Jeremy Wilusz

    Article

    Kramer et al Genes Dev. 2015 Oct 8.

    Insert

    ZKSCAN1 (ZKSCAN1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Cdk2-HA

    Plasmid

    #1884

    Depositor

    Sander van den Heuvel

    Article

    van den Heuvel et al Science 1993 Dec 24;262(5142):2050-4.

    Insert

    Cdk2 (CDK2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 14, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. 12XCSL-DsRedExpressDL

    Plasmid

    #47683

    Purpose

    Notch reporter

    Depositor

    Urban Lendahl

    Article

    Hansson et al Dev Neurosci. 2006;28(1-2):118-27.

    Insert

    TP-1 promoter
    

    Use

    Notch reporter construct

    Tags

    dsRedExpressDR

    Expression

    Mutation

    hexamerized 50-bp EBNA2 response element of the T…

    Promoter

    NA

    Available since

    Feb. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. V245 pCEP-4HA B56alpha

    Plasmid

    #14532

    Depositor

    David Virshup

    Article

    Seeling et al Science. 1999 Mar 26. 283(5410):2089-91.

    Insert

    PPP2R5A B56 alpha regulatory subunit of PP2A (PPP2R5A Human)
    

    Use

    Tags

    4 HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 23, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PGKdtabpA

    Plasmid

    #13440

    Depositor

    Philippe Soriano

    Article

    Soriano et al Development. 1997 Jul . 124(14):2691-700

    Insert

    PGKdtabpA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCAX APP C99

    Plasmid

    #30146

    Depositor

    Tracy Young-Pearse, Dennis Selkoe

    Article

    Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

    Insert

    APP C99 (APP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    C-terminal from BACE site of APP

    Promoter

    Available since

    July 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. Lox-Stop-Lox TOPO

    Plasmid

    #11584

    Purpose

    Cre-dependent activation of gene of interest by removing Stop sequences; Puro selection.

    Depositor

    Tyler Jacks

    Article

    Jackson et al Genes Dev. 2001 Dec 15. 15(24):3243-8.

    Insert

    loxP-STOP-loxP
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 22, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pX330a dCas9-LSD1

    Plasmid

    #92362

    Purpose

    Modified CAG promoter-containing vector for ubiquitous expression of catalytically inactive Cas9 fused to LSD1. For targeted enhancer demethylation in chicken embryos.

    Depositor

    Tatjana Sauka-Spengler

    Article

    Williams et al Development. 2018 Feb 23;145(4). pii: de

    Insert

    dCas9-LSD1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Aug. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pQCXIH-Myc-YAP-5SA

    Plasmid

    #33093

    Depositor

    Kunliang Guan

    Article

    Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

    Insert

    YAP (YAP1 Human)
    

    Use

    Retroviral

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    S61A, S109A, S127A, S164A, S381A (phosphorylation…

    Promoter

    CMV/MSV

    Available since

    Feb. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pDONR223-FLT3

    Plasmid

    #23895

    Depositor

    David Root, William Hahn

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    FLT3 (FLT3 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pEBG

    Plasmid

    #22227

    Purpose

    GST fusion vector

    Depositor

    David Baltimore

    Article

    Tanaka et al Mol Cell Biol. 1995 Dec . 15(12):6829-37

    Type

    Empty backbone

    Use

    Tags

    GST

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 1, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA3-myc-CUL5

    Plasmid

    #19895

    Depositor

    Yue Xiong

    Article

    Ohta et al Mol Cell. 1999 Apr . 3(4):535-41.

    Insert

    cullin 5 (CUL5 Human)
    

    Use

    Tags

    myc3

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 31, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. LAMP1-GFP

    Plasmid

    #16290

    Depositor

    Ron Vale

    Article

    Minin et al J Cell Sci. 2006 Feb 15. 119(Pt 4):659-7

    Insert

    LAMP1/RE72002
    

    Use

    Tags

    EGFP

    Expression

    Insect

    Mutation

    Promoter

    Ac5

    Available since

    March 12, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pDTA-TK

    Plasmid

    #22677

    Depositor

    Robert Benezra

    Article

    Nam et al Cell Stem Cell. 2009 Nov 6. 5(5):515-26.

    Insert

    DTA and TK
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    pDTA-TK dual selection plasmid for targeting vect…

    Promoter

    Available since

    Dec. 4, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLL7.0: Venus-iLID-CAAX (from KRas4B)

    Plasmid

    #60411

    Purpose

    Mammalian Expression of Venus-iLID-CAAX

    Depositor

    Brian Kuhlman

    Article

    Guntas et al Proc Natl Acad Sci U S A. 2015 Jan 6;112

    Insert

    Venus-iLID-CAAX
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge