159,157 results
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Plasmid#105557PurposeAAV expression of mCerulean from CB7 promoterDepositorHas ServiceAAV1 and AAV9InsertmCerulean
UseAAVTagsExpressionMammalianMutationPromoterCB7Available sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-tet-iCas9-BFP2
Plasmid#125519Purposeall-in-one-inducible Cas9DepositorInsertAAVS1-TET-ON 3G-SpCas9-P2A-BFP2
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUC19/mChe-Px
Plasmid#183170PurposeConstruct for transient expression of peroxisomes-targeted mCherry in plants.DepositorInsertmCherry-Px
UseTags10 aa peroxisome targeting signal 1 (IHHPRELSRL) …ExpressionPlantMutationPromoterAvailable sinceApril 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-IRAK4
Plasmid#23749DepositorInsertIRAK4 (IRAK4 Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
SpyCatcher003
Plasmid#133447PurposeExpresses SpyCatcher003 in bacterial cytoplasmDepositorInsertSpyCatcher003
UseTagsGSSGS, His6, and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
Lyso-B
Plasmid#209873PurposeDimerization dependent fluorescent protein B anchored to cytosolic face of the lysosomal membrane with human LAMP1DepositorInsertddGFP B
UseTagsLAMP1ExpressionMammalianMutationPromoterCMVAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE30-His-IF2
Plasmid#124124PurposeBacterial expression plasmid for PURE system componentsDepositorInsertInitiation factor 2 (infB Escherichia coli)
UseTags6x-HisExpressionBacterialMutationPromoterAvailable sinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
TFORF0172
Plasmid#142551PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertONECUT2 (ONECUT2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET16B.Pfu
Plasmid#12509PurposeBacterial expression of Pfu DNA polymeraseDepositorInsertPfu DNA polymerase
UseTagsExpressionBacterialMutationY218H, Q462R, F533L, K559I and V683A (mutations w…PromoterAvailable sinceSept. 18, 2006AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-KHK
Plasmid#23586DepositorInsertKHK (KHK Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pUC19/Pd-mChe
Plasmid#183172PurposeConstruct for transient expression of plasmodesmata-targeted mCherry in plants.DepositorInsertPd-mCherry
UseTagsAtPDCB1, A. thaliana PLASMODESMATA CALLOSE-BINDIN…ExpressionPlantMutationPromoterAvailable sinceApril 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
TFORF3383
Plasmid#144859PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertNFKBID (NFKBID Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceMay 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJOG1368
Plasmid#167301PurposeLevel 0 module (CDS1) for further assemblies based on the Modular Cloning system. FCY-UPP gene conferring sensitivity to 5-FluorocytosineDepositorInsertFCY-UPP fusion gene
UseSynthetic Biology; Moclo level 0 vectorTagsExpressionMutationPromoterAvailable sinceMay 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
TRE-UBC-HLA-E SCT-T2A-GFP
Plasmid#205461Purposelentiviral plasmid for expression of HLA-E SCTDepositorInsertHLA-E SCT (HLA-E Human)
UseTagsExpressionMammalianMutationPromoterUBCAvailable sinceOct. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
hLRP1-pEZT-BM
Plasmid#177985PurposeExpression the full-length ectodomain of human LRP1 [residue 1-4414] in mammalian cellsDepositorInsertLDL receptor related protein 1 (LRP1 Human)
UseTagsNoExpressionMammalianMutationPromoterCMVAvailable sinceDec. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHyo245
Plasmid#173147PurposeExpresses pheS_A294G by dual T7 promoter in e.coliDepositorInsertpheS_A294G
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
TFORF0662
Plasmid#143063PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertCHD6 (CHD6 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
FRP792_insul-(lexA-box)2-PminCYC1-Citrine-TCYC1
Plasmid#58433PurposeInduces Citrine expression from a promoter containing 2 lexA boxes in yeast cellsDepositorInsertCitrineA206K
UseTagsExpressionYeastMutationPromoterinsul-(lexA-box)2-PminCYC1Available sinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCFJ90 - Pmyo-2::mCherry::unc-54utr
Plasmid#19327DepositorArticleInsertPmyo-2::mCherry
UseTagsExpressionWormMutationPromoterAvailable sinceOct. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
pmGFP-P2A-K0-P2A-RFP
Plasmid#105686PurposeDouble fluorescent stalling reporter K0DepositorInsertsP2A-3xFLAG-VHPbeta
K0
P2A-mCherry
UseTagsExpressionMammalianMutationPromoterCMV (from vector)Available sinceFeb. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
TFORF0987
Plasmid#142715PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertKLF2 (KLF2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMOS007: PercevalHR ATP/ADP sensor (cytosolic)
Plasmid#163061PurposePercevalHR ATP/ADP sensor (cytosolic)DepositorInsertPercevalHR
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYES2-HTH-HHT2
Plasmid#86482Purposeyeast expression of HHT2 with His10-TEV-HA tagDepositorInsertHHT2 (HHT2 Budding Yeast)
UseTagsHIS10-TEV-HA (HTH)ExpressionYeastMutationPromoterGAL1Available sinceJan. 27, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCDNA5/FRT-ChemoG-NAD
Plasmid#193821PurposeCMV overexpression of ChemoG-NAD in mammalian cell linesDepositorInsertChemoG-NAD
UseTagsExpressionMammalianMutationEGFP:A206K, T225R; HT7: L271EPromoterCMVAvailable sinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUC19/mChe-Cs
Plasmid#183171PurposeConstruct for transient expression of cytoskeleton-targeted mCherry in plants.DepositorInsertmCherry-Cs
UseTags363 aa of A. thaliana FIMBRIN 1 (AtFIM1)ExpressionPlantMutationPromoterAvailable sinceApril 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJOG1336
Plasmid#167303PurposeLevel 0 module (3U+Ter) for further assemblies based on the Modular Cloning system. 3'UTR and flanking sequence of a Solanum lycopersicum Oleosin gene, arbitrarily called OLE2 (Sollyc03g112440)DepositorInsert3'UTR and Terminator SlOLE2
UseSynthetic Biology; Moclo level 0 vectorTagsExpressionMutationPromoterAvailable sinceMay 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJOG1334
Plasmid#167302PurposeLevel 0 module (Pro+5U) for further assemblies based on the Modular Cloning system. Promoter and 5'UTR of a Solanum lycopersicum Oleosin gene, arbitrarily called OLE2 (Sollyc03g112440)DepositorInsertPromoter SlOLE2
UseSynthetic Biology; Moclo level 0 vectorTagsExpressionMutationPromoterAvailable sinceMay 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPOTv7-blast-10Myc
Plasmid#181939PurposeTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene taggingDepositorInsert10xMyc
UseUnspecifiedTagsExpressionMutationPromoterread throughAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBabepuro-IL8
Plasmid#189608PurposeRetroviral expression vector for IL8DepositorInsertIL8 (CXCL8 Human)
UseRetroviralTagsExpressionMutationPromoterSV40 early promoterAvailable sinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-TO-dCas9-3XmCherry
Plasmid#64108PurposeExpression of Sp dCas9-3XmCherry in mammalian cellsDepositorInsertSp dCas9
UseCRISPR and LentiviralTags3XmCherryExpressionMammalianMutationPromoterCMV-TOAvailable sinceMay 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUC19/mChe-Golgi
Plasmid#183169PurposeConstruct for transient expression of Golgi apparatus-targeted mCherry in plants.DepositorInsertmCherry-Golgi
UseTagsAtRER1B, A. thaliana endoplasmic reticulum retrie…ExpressionPlantMutationPromoterAvailable sinceApril 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFastBac HT JS-Munc18c
Plasmid#135555PurposeExpress mouse Munc18c in Sf9 cells, the resulted plasmid encodes an N-terminally His6-tagged Munc18c protein with a tobacco etch virus (TEV) cleavage site between the His6 tag and Munc18c.DepositorInsertMunc18c
UseTags6xHis tag and TEV siteExpressionInsectMutationPromoterpolyhedrin promoterAvailable sinceFeb. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC19-link-sfGFP-FLAG:Kan
Plasmid#211833PurposePlasmid template that encodes the fluorescent protein sfGFP with a FLAG tag next to kanamycin resistance gene. This template was used to copy out sfGFP-FLAG-Kan for genetic tagging.DepositorInsertsfGFP-FLAG-Kan
UseTagsExpressionBacterialMutationNAPromoterAvailable sinceJan. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
LXRE_Luc
Plasmid#177622Purposesynthetic 3X LXRE promoter linked to luciferase in the pTAL vectorDepositorInsert3X LXRE
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceApril 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRU43
Plasmid#167665PurposeENTRY vector for gRNA3 cloning under pAtU6 promoterDepositorInsertpAtU6
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV_BiPVe3_dTomato_nlsdTomato
Plasmid#213940PurposeAAV construct expressing dTomato driven by PV+ basket cell-targeting enhancer. Alias: pAAV_WP0003_dTomato_nlsdTomatoDepositorHas ServiceAAV PHP.eBInsertdTomato_P2A_nls.dTomato
UseAAVTagsExpressionMutationPromoterAvailable sinceJuly 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV GFP Hygro (656-4)
Plasmid#17446Purpose3rd gen lentiviral eGFP expression vector, CMV promoter, HygroDepositorHas ServiceLentiviral PrepInsertGFP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pcw57.1-RquA-FLAG
Plasmid#229685PurposeDoxycycline-inducible expression of RquA-FLAGDepositorInsertRquA-FLAG
UseLentiviralTagsExpressionMammalianMutationPromoterTREAvailable sinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
CALM-pmCherryN1
Plasmid#27691DepositorInsertCALM (Picalm Mouse)
UseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceMay 4, 2011AvailabilityAcademic Institutions and Nonprofits only -
p-miniTurbo
Plasmid#209636PurposeExpression miniTurbo in mammalian cellsDepositorInsertminiTurbo
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-CD80-mTFP1
Plasmid#223605PurposeLentiviral expression of human CD80 with mTFP1 tag in mammalian cellsDepositorInsertCD80 (CD80 Human)
UseLentiviral; mammalian expressionTagsmTFP1ExpressionMutationPromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
TFORF3432
Plasmid#144908PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertNR3C1 (NR3C1 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
TVBB C-term-mScarlet
Plasmid#169219PurposeTargeting vector backbone to support a knock-in of Linker-mScarlet at the C-terminus of a target locusDepositorInsertDouble SapI flanked mScarlet
UseTargeting vector backboneTagsExpressionMutationPromoterAvailable sinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUltra-puro-RTTA3
Plasmid#58750Purposesilence doxycyclin inducible pUltra-doxDepositorInsertReverse TET-Transactivator 3
UseLentiviralTagsExpressionMammalianMutationloxP5171PromoterUbiquitin C promoterAvailable sinceOct. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pIVT/CamKII gamma CA
Plasmid#122142PurposeFor making constitutively active CamKII gamma RNADepositorInsertconstitutively active CamKII gamma (Camk2g Mouse)
UseFor in vitro transcriptionTagsExpressionMutationFirst 291 amino acid residues of CaMKII gammaPromoterT7Available sinceMarch 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
PX459v3
Plasmid#178799PurposeSpCas9 with 2A-Puro, and a golden gate cloning backbone for sgRNA. sgRNA scaffold seqeuence has been modified for increased sgRNA expression (v3).DepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterCbh (Cas9-2A Puro) and U6 (gRNA)Available sinceAug. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CBA.loxP.ArcLightD.2A.nlsmCherry.loxP.WPRE.SV40
Plasmid#100030PurposeAAV expression of ArcLightDDepositorInsertArcLightD
UseAAVTags2A-mCherryExpressionMammalianMutationPromoterCBAAvailable sinceJan. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
1B-GFP1-10
Plasmid#193867PurposeE. coli expression vector for Hisx6 tagged GFP1-10.DepositorInsertsfGFP1-10
UseTags6xHisExpressionBacterialMutationPromoterT7 PromoterAvailable sinceAug. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQCXIP-VEGFC
Plasmid#73012PurposeRetroviral expression of human VEGFCDepositorInsertVEGFC (VEGFC Human)
UseRetroviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
Apple-53BP1trunc
Plasmid#69531PurposeFluorescent reporter for DNA damageDepositorInsert53BP1 (TP53BP1 Human)
UseLentiviralTagsApple fluorescent proteinExpressionMammalianMutationPromoterCMVAvailable sinceOct. 2, 2015AvailabilityAcademic Institutions and Nonprofits only