We narrowed to 14,123 results for: cas9 genes
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Plasmid#188484PurposegRNA plasmid with neomycin resistance expressing a single guide RNA targeting cytomegaloviral promoter site 51.DepositorInsertCMV promoter sgRNA
UseCRISPRExpressionMammalianMutationNonePromoterU6Available SinceAug. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
rtTA-sg-mGreenLantern/EGFP
Plasmid#188482PurposegRNA plasmid with neomycin resistance expressing a single guide RNA targeting mGreenLantern and EGFP fluorescent proteins.DepositorInsertmGreenLantern/EGFP sgRNA
UseCRISPRExpressionMammalianMutationNonePromoterU6Available SinceAug. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMC0x_gRNA_Pos5b_Ptet
Plasmid#179338PurposegRNA Position Vector 5b, used to create multi gRNA NT-CRISPR plasmid with pST_119_LVL2 camDepositorInsertPtet gRNA with sfGFP dropout
UseCRISPRAvailable SinceMarch 21, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMC0x_gRNA_Pos5a_Ptet
Plasmid#179337PurposegRNA Position Vector 5a, used to create multi gRNA NT-CRISPR plasmid with pST_119_LVL2 camDepositorInsertPtet gRNA with sfGFP dropout
UseCRISPRAvailable SinceMarch 21, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAC-U63-tgRNA-Gal80
Plasmid#169030PurposegRNA-marker vector contain a U6:3 promoter, gRNA scaffold, and a Ubi-Gal80 marker.DepositorTypeEmpty backboneUseCRISPRExpressionInsectPromoterU6:3Available SinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYMCR2_Gb_PMLTSS
Plasmid#140285PurposeCRISPR/Cas9 plasmids encoding sgRNA targeting TSS of PML for knock-inDepositorInsertCas9
ExpressionMammalianPromoterCBHAvailable SinceMay 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRS424-ysg(NT)
Plasmid#138258PurposeExpression of a non-targeting sgRNA to be used as a control in a yeast dual reporter systemDepositorInsertNontargeting sgRNA
UseCRISPRExpressionYeastPromoterSNR52Available SinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSB1C3-ysg(NT)
Plasmid#138263PurposeSubcloning and expression of non-targeting sgRNA for use in yeast dual reporter system.DepositorInsertNontargeting sgRNA
UseCRISPRExpressionYeastPromoterSNR52Available SinceMarch 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR-R4R3-mRuby-N
Plasmid#113752PurposeGateway multi-site entry clone for second position mRuby (N-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.DepositorInsertmRuby2
UseCRISPR; Gateway entry vectorTagsmRuby2Available SinceNov. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR-R4R3-2xmEGFP-N
Plasmid#113747PurposeGateway multi-site entry clone for second position 2x mEGFP (N-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.DepositorInsertmEGFP-mEGFP
UseCRISPR; Gateway entry vectorTags2xmEGFPAvailable SinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR-R4R3-3xmEGFP-N
Plasmid#113748PurposeGateway multi-site entry clone for second position 3x mEGFP (N-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.DepositorInsertmEGFP-mEGFP-mEGFP
UseCRISPR; Gateway entry vectorTags3xmEGFPAvailable SinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR-R4R3-2xmEGFP-C
Plasmid#113750PurposeGateway multi-site entry clone for second position 2x mEGFP (C-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.DepositorInsertmEGFP-mEGFP
UseCRISPR; Gateway entry vectorTags2xmEGFPAvailable SinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR-R4R3-2xmRuby-N
Plasmid#113753PurposeGateway multi-site entry clone for second position 2x mRuby (N-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.DepositorInsertmRuby2-mRuby2
UseCRISPR; Gateway entry vectorTags2xmRuby2Available SinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR-R4R3-2xmRuby-C
Plasmid#113756PurposeGateway multi-site entry clone for second position 2x mRuby (C-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.DepositorInsertmRuby2-mRuby2
UseCRISPR; Gateway entry vectorTags2xmRuby2Available SinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCfB4779
Plasmid#106145PurposeEasyCloneYALI system-based yeast integrative vector carrying loxP-flanked nourseothricin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntE_2, USER site for cloning, amp resistanceDepositorTypeEmpty backboneExpressionYeastAvailable SinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCfB4784
Plasmid#106148PurposeEasyCloneYALI system-based yeast integrative vector carrying loxP-flanked nourseothricin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntF_2, USER site for cloning, amp resistanceDepositorTypeEmpty backboneExpressionYeastAvailable SinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCfB6637
Plasmid#106164PurposeEasyCloneYALI system-based yeast gRNA expression vector carrying a nourseothricin-resistance marker, helps to integrate vector pCfB6681 into Yarrowia lipolytica chromosomal location IntE_3, amp resistanceDepositorInsertunknown
ExpressionYeastAvailable SinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCfB4785
Plasmid#106149PurposeEasyCloneYALI system-based yeast integrative vector carrying loxP-flanked nourseothricin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntF_3, USER site for cloning, amp resistanceDepositorTypeEmpty backboneExpressionYeastAvailable SinceApril 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCfB4783
Plasmid#106146PurposeEasyCloneYALI system-based yeast integrative vector carrying loxP-flanked nourseothricin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntE_3, USER site for cloning, amp resistanceDepositorTypeEmpty backboneExpressionYeastAvailable SinceApril 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCfB4781
Plasmid#106140PurposeEasyCloneYALI system-based yeast integrative vector carrying loxP-flanked nourseothricin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntC_1, USER site for cloning, amp resistanceDepositorTypeEmpty backboneExpressionYeastAvailable SinceApril 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCfB4788
Plasmid#106139PurposeEasyCloneYALI system-based yeast integrative vector carrying loxP-flanked nourseothricin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntA_1, USER site for cloning, amp resistanceDepositorTypeEmpty backboneExpressionYeastAvailable SinceApril 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
EMX1*_sgRNA
Plasmid#100558PurposeExpresses EMX1* sgRNA. Target sequence: (G)GAGTCCGAGCAGAAGAAGAA. Same target sequence as #100555, except with an additional 5' G.DepositorInsertEMX1 sgRNA
PromoterU6Available SinceSept. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
AP553‐1
Plasmid#87240Purposegtbp‐1 (~500 bp flanking sequence)::GFP (with 3 introns)::gtbp‐1 (~500 bp flanking sequence)DepositorInsertgtbp‐1 (~500 bp flanking sequence)::GFP (with 3 introns)::gtbp‐1 (~500 bp flanking sequence)
Available SinceFeb. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHU6_Ch5_155183064-gRNA-for
Plasmid#81214PurposepHu6-gRNA-NT1_SpecR backbone, gRNA for targeting the 3' region of pCALNL_Ch5 reporterDepositorInserthU6 expression of gRNA
ExpressionMammalianPromoterU6Available SinceNov. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHU6_Ch5_155183064-gRNA-rev
Plasmid#81215PurposepHu6-gRNA-NT1_SpecR backbone, gRNA for targeting the 5' region of pCALNL_Ch5 reporterDepositorInserthU6 expression of gRNA
ExpressionMammalianPromoterU6Available SinceNov. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
sgRNA1_pNeurog2-Luciferase reporter
Plasmid#64160PurposePhotoactivatable transcription system. Mammalian expression of sgRNA1 to target pNeurog2-Luciferase reporter.DepositorInsertsgRNA1 for pNeurog2-Luciferase reporter
UseCRISPRExpressionMammalianPromoterhuman U6Available SinceJune 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pX330A_FokI-1x5
Plasmid#63605PurposeExpresses FokI-dCas9 and gRNADepositorInsertFokI-dCas9
UseCRISPRExpressionMammalianPromoterCBhAvailable SinceJune 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCAG-CBE4max-SpRYc-P2A-EGFP
Plasmid#208336PurposeCAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpRYcDepositorInserthuman codon optimized CBE4max Cas9 variant named SpRYc with P2A-EGFP
UseCRISPRTagsP2A-EGFPExpressionMammalianMutationSc++ (1-1119), SpRY (1111-1368), D10APromoterCAGAvailable SinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEJS1096 Dual-sgRNA.Design 1
Plasmid#159538PurposeDelivery of dual sgRNA cassettes and Nme2Cas9 in a single AAV vectorDepositorInsertNme2Cas9 nuclease with two guide RNA cassettes with promoters
UseAAV, CRISPR, and Mouse TargetingTagsNLSExpressionMammalianPromoterU1aAvailable SinceDec. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGTRm
Plasmid#127197PurposepGTRm is the template plasmid for PCR & golden gate assembly driven generation of polycistronic tRNA-gRNA sequencesDepositorInserttRNA-gRNA PCR template
UseTemplate plasmid for generation of golden gate pc…PromoterNoneAvailable SinceMay 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
sg2
Plasmid#113967PurposeSingle short guide RNA targeting TACCACATTTGTAGAGGTTDepositorInsertsg2
ExpressionMammalianPromoterU6Available SinceMay 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
sg3
Plasmid#113968PurposeSingle short guide RNA targeting CAATGTATCTTATCATGTCDepositorInsertsg3
ExpressionMammalianPromoterU6Available SinceMay 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
sg2+sg3
Plasmid#113970PurposeDouble short guide RNA targeting TACCACATTTGTAGAGGTT & CAATGTATCTTATCATGTCDepositorInsertsg2+sg3
ExpressionMammalianPromoterU6Available SinceMay 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEdit
Plasmid#232355PurposeThe pEdit series plasmids are derived from the pTarget series plasmids by inserting homologous recombination arms targeting the gene of interest, which enables gene knockout or gene insertion at the tDepositorInsertsgRNA
UseCRISPRAvailable SinceNov. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAf-CRISPR-yA
Plasmid#191015PurposeCRISPR vector based on Aspergillus flavus U6 promoter and terminator for targeting the yA gene, which contains AMA1(the HindIII-PstI fragment) and ptrA selection marker.DepositorInsertyA
UseCRISPRPromoterAspergillus flavus U6Available SinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
PL552
Plasmid#68407PurposeDonor empty vector with floxed puromycin resistance gene expression cassetteDepositorTypeEmpty backboneUseCre/LoxExpressionMammalianPromotermouse PGK promoterAvailable SinceNov. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAf-CRISPR-phoA
Plasmid#207991PurposeCRISPR vector used with pAf-CRISPR-ctfR1 (#207992) to delete both aflatoxin and cyclopiazonic acid gene clusters of Aspergillus flavusDepositorInsertphoA
UseCRISPRPromoterAspergillus flavus U6Available SinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHYG
Plasmid#243678PurposeExpresses the HYGROMYCIN PHOSPHOTRANSFERASE (HPT) CDS in plants using the Cestrum Yellow Leaf Curling Virus (CmYLCV) 9.11 promoter and an Arabidopsis HSP terminatorDepositorInsertHYGROMYCIN PHOSPHOTRANSFERASE (HPT) CDS
ExpressionPlantAvailable SinceNov. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAf-CRISPR-ctfR1
Plasmid#207992PurposeCRISPR vector used with pAf-CRISPR-phoA (#207991) to delete both aflatoxin and cyclopiazonic acid gene clusters of Aspergillus flavusDepositorInsertctfR1
UseCRISPRPromoterAspergillus flavus U6Available SinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2CG2-U6:gRNA
Plasmid#63156PurposeDestination vector for Gateway based on vector #395 from the Tol2Kit, containing a gRNA scaffold under the control of a zebrafish U6 promoter, and cmlc2:GFP as a transgenesis marker.DepositorTypeEmpty backboneUseCRISPR; Tol2 destination vector for gatewayAvailable SinceApril 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAGM9037
Plasmid#153225PurposeTemplate for PCR amplification of the constant part of guide RNAsDepositorInsertguide RNA constant part, carotenoid marker
UseSynthetic BiologyAvailable SinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
AD59_pEP.DonorCLYBL.TS
Plasmid#199227PurposeDonor plasmid with CLYBL target sites for ITPN or HMEJ knock-in at the human CLYBL safe harbor locusDepositorInsertExpression unit for PuroR.T2A.EGFP selectable and reporter markers
UseGene targeting donor plasmidExpressionMammalianPromoterHuman PGK1 gene promoterAvailable SinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
PB-SAM-Dest
Plasmid#102563PurposePiggybac transposon DEST vector encoding dCAS9-VP64 and MS2-P65-HSF1 activator helper complex. It is used for insertion of multiple U6-sgRNA expression cassettes.DepositorTypeEmpty backboneUseCRISPR; Piggybac transposonExpressionMammalianPromoterN/AAvailable SinceJan. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2pA2-U6:gRNA
Plasmid#63157PurposeDestination vector for Gateway based on vector #394 from the Tol2Kit, containing a gRNA scaffold under the control of a zebrafish U6 promoter.DepositorTypeEmpty backboneUseCRISPR; Tol2 destination vector for gatewayAvailable SinceApril 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBHM2329
Plasmid#171686PurposepRS316-PCnU6-scaffold, contains template for amplification of PCnU6 and scaffold for fusion PCRDepositorInsertPCnU6-sgRNA_scaffold
UseCRISPRExpressionBacterial and YeastPromoterC. neoformans U6Available SinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSC5
Plasmid#104779PurposepSC218GG-Glycine max Ubiquitin:Gateway expression cassette binary vectorDepositorTypeEmpty backboneUseCRISPRExpressionPlantAvailable SinceJan. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
Lenti-entry-puro
Plasmid#85745PurposeLentiviral vector for generating multiple sgRNAs carrying plasmids by Golden Gate Assembly.DepositorTypeEmpty backboneUseLentiviralAvailable SinceJan. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAGM38869
Plasmid#153220PurposeLevel 0 Arabidopsis U6-promoter vectorDepositorInsertArabidopsis U6 snRNA promoter
UseSynthetic BiologyAvailable SinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRN227
Plasmid#127222PurposeBeYDV replicon with 35S::Cas9, WUS2 and AtSTM, sgRNA targeting PDSDepositorInsertCas9, WUS2, AtSTM, sgRNA
ExpressionPlantMutationcodon optimized CDSsAvailable SinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAGM4723:TpCC_Urease
Plasmid#85982PurposeGolden Gate Level 2 construct encoding Cas9-YFP and 2 urease-targeting gRNAsDepositorInsertsCas9
Urease-targeting gRNA 1
Urease-targeting gRNA 2
UseCRISPR; Diatom (t. pseudonana)TagsNLS and YFPPromoterFCP and U6Available SinceJan. 18, 2017AvailabilityAcademic Institutions and Nonprofits only