We narrowed to 9,308 results for: control

1. Hy_pMT Flag-Hel25E K91N

   Plasmid

   #110121

   Purpose

   Expresses Flag-tagged D. melanogaster Hel25E with K91N mutation that greatly reduces helicase activity

   Depositor

   Jeremy Wilusz

   Article

   Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

   Insert

   Hel25E (Hel25E Fly)
   

   Tags

   Flag

   Expression

   Insect

   Mutation

   K91N mutation

   Promoter

   Metallothionein Promoter (pMT)

   Available Since

   Sept. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. Hy_pMT Flag-Hel25E D193E

   Plasmid

   #110120

   Purpose

   Expresses Flag-tagged D. melanogaster Hel25E with D193E mutation that greatly reduces ATP binding affinity

   Depositor

   Jeremy Wilusz

   Article

   Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

   Insert

   Hel25E (Hel25E Fly)
   

   Tags

   Flag

   Expression

   Insect

   Mutation

   D193E mutation

   Promoter

   Metallothionein Promoter (pMT)

   Available Since

   Sept. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSG5-HA-H-Ras_wt

   Plasmid

   #39509

   Depositor

   Julian Downward

   Article

   Rodriguez-Viciana et al Cell. 1997 May 2;89(3):457-67.

   Insert

   HRAS (HRAS Human)
   

   Use

   Eukaryotic

   Tags

   HA

   Available Since

   Aug. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

4. pJET-41-DExCon-antiGFPnanobody-mCherry-R11b

   Plasmid

   #179901

   Purpose

   Donor with homologous arms for Rab11b to knock in DExCon-antiGFPnanobody-mCherry module

   Depositor

   Patrick Caswell

   Article

   Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

   Insert

   RAB11B, member RAS oncogene family (RAB11B Human)
   

   Use

   CRISPR; Donor for homologous based knock in

   Tags

   antiGFPnanobody-mCherry

   Expression

   Bacterial and Mammalian

   Promoter

   TRE3GS

   Available Since

   May 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJET-07-mNeonGreen-R11a

   Plasmid

   #179890

   Purpose

   Donor with homologous arms for Rab11a to knock in mNeonGreen

   Depositor

   Patrick Caswell

   Article

   Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

   Insert

   RAB11A, member RAS oncogene family (RAB11A Human)
   

   Use

   CRISPR; Donor for homologous based knock in

   Tags

   mNeonGreen

   Expression

   Bacterial and Mammalian

   Available Since

   May 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-HA-TFAP4(E135A/S139A)

   Plasmid

   #110157

   Purpose

   Expression of human TFAP4 (E135A/S139A) in mammalian cells

   Depositor

   Daniele Guardavaccaro

   Article

   D'Annibale et al J Biol Chem. 2014 Mar 14;289(11):7730-7.

   Insert

   TFAP4 (transcription factor AP-4) (TFAP4 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   E135A/S139A

   Promoter

   CMV

   Available Since

   May 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGP-CMV-GCaMP3-44

   Plasmid

   #91780

   Purpose

   Mammalian expression of low affinity calcium-sensing fluorescent protein

   Depositor

   GENIE Project, Douglas Kim

   Article

   de Juan-Sanz et al Neuron. 2017 Jan 30. pii: S0896-6273(17)

   Insert

   GCaMP3-44
   

   Tags

   6xHis, T7 epitope, and Xpress tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDNA5-FRT-TO-SSPB(micro)-mVenus-GCN4-ppKin14VIb(861-1321)_P2A_iLID-mCherry-RAB11

   Plasmid

   #174647

   Purpose

   Optogenetic coupling of RAB11 to tetramerized moss kinesin-14 to induce retrograde transport of recycling endosomes. Compatible with Flp-in TREX system.

   Depositor

   Lukas Kapitein

   Article

   Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

   Insert

   SSPB(micro)-mVenus-GCN4-ppKin14Vib(816-1321)-P2A-iLID-mCherry-RAB11
   

   Expression

   Mammalian

   Mutation

   SSPB:Arg73Gln; mVenus: Met1Del, Thr154Met; ppKin1…

   Promoter

   CMV (with TetOn)

   Available Since

   Nov. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPHR-mCherry-NLS-VP16

   Plasmid

   #103821

   Purpose

   Soluble BLInCR transcription activator (transactivation domain of viral VP16 protein) that is recruited to 'localizer' sites upon blue light illumination

   Depositor

   Karsten Rippe

   Article

   Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

   Insert

   PHR-mCherry-VP16 (CRY2 Mustard Weed)
   

   Expression

   Mammalian

   Mutation

   VP16: G126T (silent, G42G)

   Promoter

   CMV

   Available Since

   May 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPHR-iRFP713-NLS-VP16

    Plasmid

    #103823

    Purpose

    Soluble BLInCR transcription activator (transactivation domain of viral VP16 protein) that is recruited to 'localizer' sites upon blue light illumination

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    PHR-iRFP713-VP16 (CRY2 Mustard Weed)
    

    Expression

    Mammalian

    Mutation

    VP16: G126T (silent, G42G)

    Promoter

    CMV

    Available Since

    Dec. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. p21 promoter E

    Plasmid

    #16466

    Depositor

    Bert Vogelstein

    Article

    el-Deiry et al Cancer Res. 1995 Jul 1. 55(13):2910-9.

    Insert

    p21 promoter (CDKN1A Human)
    

    Expression

    Mammalian

    Available Since

    April 7, 2008

    Availability

    Academic Institutions and Nonprofits only

12. pRRLNeo-pEF1a-p53ash-mKate2-splitmVenusN

    Plasmid

    #69582

    Purpose

    Expresses p53 tagged with mKate2 and split N-term mVenus

    Depositor

    Galit Lahav

    Article

    Gaglia et al Mol Syst Biol. 2014 Oct 24;10:753. doi:

    Insert

    p53 (TP53 Human)
    

    Use

    Lentiviral

    Tags

    mKate2 and split mVenus - N term

    Expression

    Mammalian

    Mutation

    7 silent mutations in p53 DNA to prevent targetin…

    Promoter

    EF1alpha

    Available Since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pENTR-AtTAS1c-D2-B/c

    Plasmid

    #137883

    Purpose

    Gateway-compatible entry vector for direct cloning of synthetic trans-acting siRNAs into Arabidopsis thaliana TAS1c precursor at position downstream 3'D1[+]

    Depositor

    Alberto Carbonell

    Article

    Lopez-Dolz et al Nucleic Acids Res. 2020 May 12. pii: 583

    Insert

    AtTAS1c-D2-B/c
    

    Use

    Gateway-compatible entry clone

    Mutation

    A. thaliana TAS1c precursor sequence including a …

    Available Since

    May 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pX4

    Plasmid

    #183903

    Purpose

    donor plasmid for CRISPR Cas9 knockin near the Aedes aegypti m / M locus

    Depositor

    Eric Marois

    Article

    Lutrat et al Commun Biol. 2023 Jun 16;6(1):646. doi:

    Insert

    GFP
    

    Expression

    Insect

    Promoter

    Aedes aegypti PUb

    Available Since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pX3

    Plasmid

    #183904

    Purpose

    donor plasmid for CRISPR Cas9 knockin near the Aedes aegypti m / M locus

    Depositor

    Eric Marois

    Article

    Lutrat et al Commun Biol. 2023 Jun 16;6(1):646. doi:

    Insert

    GFP
    

    Expression

    Insect

    Promoter

    Aedes aegypti Polyubiquitin

    Available Since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMK233 (AAVS1 CMV-AtAFB2)

    Plasmid

    #140537

    Purpose

    AAVS1 CMV-AtAFB2

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    CMV-AtAFB2
    

    Expression

    Mammalian

    Available Since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFL-SV40-Rac1 5'-UTR

    Plasmid

    #115354

    Purpose

    firefly luciferase (Fluc) reporter containing the 5’-UTR of Rac1

    Depositor

    Ming-Chih Lai

    Article

    Ku et al Mol Cell Biol. 2018 Dec 11;39(1). pii: M

    Insert

    Rac1 5'-UTR (RAC1 Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Available Since

    Sept. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGex4t2 with 244-458AA SUUR fragment

    Plasmid

    #112989

    Purpose

    Expresses fusion of GST and amino acids 244-458 of Drosophila SUUR

    Depositor

    Terry Orr-Weaver

    Article

    Nordman et al Cell Rep. 2014 Nov 6;9(3):841-9. doi: 10

    Insert

    SuUR (SuUR Fly)
    

    Expression

    Bacterial

    Mutation

    amino acids 244-458

    Available Since

    Sept. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. DT034A

    Plasmid

    #159395

    Purpose

    Constitutive LuxR expression, controlled under E.coli hybrid (J23117-J23106) promoter, and inducible Perfringolysin O (PFO) expression, controlled under V. fischeri HSL-regulated pLux (luxPR) promoter

    Depositor

    Sheref Mansy

    Article

    Toparlak et al Sci Adv. 2020 Sep 18;6(38). pii: 6/38/ea

    Insert

    pr.J23117/J23106-LuxR-pr.LuxR-PFO-pdt
    

    Use

    Synthetic Biology

    Tags

    FLAG/FLAG and pdt#3 from Collins Nat. Biotech. 20…

    Expression

    Bacterial

    Promoter

    J23117 and pLux promoter

    Available Since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. p21 promoter C

    Plasmid

    #16464

    Depositor

    Bert Vogelstein

    Article

    el-Deiry et al Cancer Res. 1995 Jul 1. 55(13):2910-9.

    Insert

    p21 promoter (CDKN1A Human)
    

    Expression

    Mammalian

    Available Since

    April 7, 2008

    Availability

    Academic Institutions and Nonprofits only

21. pPBpuro-mNG-eDHFR(69K6)-Tiam1

    Plasmid

    #172102

    Purpose

    Mammalian expression of Tiam1(DH-PH) fused to mNeonGreen-eDHFR(69K6)

    Depositor

    Shinya Tsukiji

    Article

    Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

    Insert

    mNeonGreen-eDHFR(69K6)-Tiam1(DH-PH) (TIAM1 Human)
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    July 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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22. p21 promoter B

    Plasmid

    #16463

    Depositor

    Bert Vogelstein

    Article

    el-Deiry et al Cancer Res. 1995 Jul 1. 55(13):2910-9.

    Insert

    p21 promoter (CDKN1A Human)
    

    Expression

    Mammalian

    Available Since

    April 7, 2008

    Availability

    Academic Institutions and Nonprofits only

23. p21 promoter G

    Plasmid

    #16468

    Depositor

    Bert Vogelstein

    Article

    el-Deiry et al Cancer Res. 1995 Jul 1. 55(13):2910-9.

    Insert

    p21 promoter (CDKN1A Human)
    

    Expression

    Mammalian

    Available Since

    April 7, 2008

    Availability

    Academic Institutions and Nonprofits only

24. pPHR-EYFP-hPMLIII

    Plasmid

    #103825

    Purpose

    Soluble BLInCR effector that is recruited to 'localizer' sites upon blue light illumination

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    PHR-EYFP-hPMLIII (CRY2, PML Human, Mustard Weed)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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25. pcDNA3 RL23Cm6

    Plasmid

    #109367

    Purpose

    Human rod opsin chimera with intracellular loops 2-3 and C-terminus from mGluR6

    Depositor

    Robert Lucas

    Article

    Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

    Insert

    Rod opsin-mGluR6 chimera (RHO, GRM6 Human)
    

    Tags

    1D4

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pSpCas9_BB_2A-GFP_MAPRE1-gRNA#3

    Plasmid

    #107728

    Purpose

    Knock out of EB1 in human cells by CRISPR/Cas9

    Depositor

    Torsten Wittmann

    Article

    van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

    Insert

    MAPRE1 gRNA #3 (targets Exon 1) (MAPRE1 Human)
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pFL-SV40-GNB2 5'-UTR

    Plasmid

    #115355

    Purpose

    firefly luciferase (Fluc) reporter containing the 5’-UTR of GNB2

    Depositor

    Ming-Chih Lai

    Article

    Ku et al Mol Cell Biol. 2018 Dec 11;39(1). pii: M

    Insert

    GNB2 5'-UTR (GNB2 Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Available Since

    Sept. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA3-HA-RIPK3 S199A

    Plasmid

    #78806

    Purpose

    To overexpress RIPK3 S199A in Mammalian Cells

    Depositor

    Jaewhan Song

    Article

    Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

    Insert

    RIPK3 (RIPK3 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    S199A

    Promoter

    CMV

    Available Since

    June 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. Lenti_CRISPRi_sgNeg2

    Plasmid

    #166999

    Purpose

    Lentiviral expression of negative control sgRNA for CRISPRi knockdown

    Depositor

    Gian-Paolo Dotto

    Article

    Ma et al J Exp Med. 2021 Feb 1;218(2). pii: 21150

    Insert

    Negative control sgRNA 2
    

    Use

    CRISPR and Lentiviral

    Promoter

    U6

    Available Since

    April 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. px330-RPL26 sgRNA2

    Plasmid

    #134642

    Purpose

    contains sgRNA targeting to the C-terminus of human RPL26 for gene editing

    Depositor

    Yihong Ye

    Article

    Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

    Insert

    RPL26 sgRNA2 (RPL26 Human)
    

    Expression

    Mammalian

    Available Since

    Dec. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. px330-RPL26 sgRNA1

    Plasmid

    #134641

    Purpose

    contains sgRNA targeting to the C-terminus of human RPL26 for gene editing

    Depositor

    Yihong Ye

    Article

    Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

    Insert

    RPL26 sgRNA2 (RPL26 Human)
    

    Expression

    Mammalian

    Available Since

    Dec. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pB80-KIF1A(1-383)-GFP-SSPB(nano)

    Plasmid

    #174628

    Purpose

    Constitutively active KIF1A for optogenetic heterodimerization to iLID via SSPB(nano)

    Depositor

    Lukas Kapitein

    Article

    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

    Insert

    KIF1A(1-383)-GFP-SSPB(nano) (Kif1a Mouse, Synthetic)
    

    Tags

    GFP-SSPB(nano)

    Expression

    Mammalian

    Mutation

    mmKIF1A(aa1-383): Pro202Ala; EGFP: Met1Del;

    Promoter

    Chicken beta-actin

    Available Since

    Nov. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pcDNA3-HA-RIPK3 K55R

    Plasmid

    #78807

    Purpose

    To overexpress RIPK3 K55R in Mammalian Cells

    Depositor

    Jaewhan Song

    Article

    Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

    Insert

    RIPK3 (RIPK3 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K55R

    Promoter

    CMV

    Available Since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. p21 promoter D

    Plasmid

    #16465

    Depositor

    Bert Vogelstein

    Article

    el-Deiry et al Cancer Res. 1995 Jul 1. 55(13):2910-9.

    Insert

    p21 promoter (CDKN1A Human)
    

    Expression

    Mammalian

    Available Since

    April 7, 2008

    Availability

    Academic Institutions and Nonprofits only

35. pB80-KIF1A(1-365)-GFP-SSPB(micro)

    Plasmid

    #174629

    Purpose

    Non-processive monomeric KIF1A for optogenetic heterodimerization to iLID via SSPB(micro)

    Depositor

    Lukas Kapitein

    Article

    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

    Insert

    KIF1A(1-365)-GFP-SSPB(micro) (Kif1a Mouse, Synthetic)
    

    Tags

    GFP-SSPB(micro)

    Expression

    Mammalian

    Mutation

    mmKIF1A(aa1-365): Pro202Ala; EGFP: Met1Del; SSPB:…

    Promoter

    Chicken beta-actin

    Available Since

    Nov. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pcDNA3-Ro2

    Plasmid

    #24503

    Depositor

    Bruce Conklin

    Article

    Coward et al Proc Natl Acad Sci U S A. 1998 Jan 6;95(

    Insert

    RASSL Ro2 (OPRK1 Human)
    

    Tags

    FLAG and NLS from prolactin

    Expression

    Mammalian

    Mutation

    mutations in extracellular loops 2 (see comments …

    Available Since

    June 16, 2010

    Availability

    Academic Institutions and Nonprofits only

37. Bcr GAP domain/pGEX 3X

    Plasmid

    #36405

    Depositor

    Nora Heisterkamp

    Article

    Cho et al Mol Cell Biol. 2007 Feb;27(3):899-911. E

    Insert

    Bcr encoding residues 1004-1271 (BCR Human)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    July 19, 2012

    Availability

    Academic Institutions and Nonprofits only

38. pSG5-H-Ras_V12

    Plasmid

    #39510

    Depositor

    Julian Downward

    Article

    Rodriguez-Viciana et al Cell. 1997 May 2;89(3):457-67.

    Insert

    HRAS V12 (HRAS Human)
    

    Use

    Eukaryotic

    Mutation

    G12V

    Available Since

    Aug. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

39. pcDNA3-FLAG-hDIXDC1 S592A

    Plasmid

    #61223

    Purpose

    Mammalian expression vector encoding long isoform of human DIXDC1

    Depositor

    Reuben Shaw

    Article

    Goodwin et al Mol Cell. 2014 Aug 7;55(3):436-50. doi:

    Insert

    human DIXDC1 (DIXDC1 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Changed Serine 592 to Alanine

    Promoter

    CMV

    Available Since

    Jan. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

40. pFastBac-His6-Mps1-KD

    Plasmid

    #114064

    Purpose

    protein production

    Depositor

    Geert Kops

    Article

    Jelluma et al Cell. 2008 Jan 25;132(2):233-46. doi: 10

    Insert

    Mps1-KD (TTK Human)
    

    Tags

    His6

    Expression

    Insect

    Mutation

    Mps1 KD (D664A)

    Available Since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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