We narrowed to 29,705 results for: LIS
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HA-HIF2α wt-pBabe-Puro
Plasmid#26055DepositorInsertHypoxia inducible factor 2 alpha (EPAS1 Human)
UseRetroviralTagsHA-tagExpressionMammalianMutationwt cDNAPromoterAvailable sinceAug. 20, 2010AvailabilityAcademic Institutions and Nonprofits only -
pLenti-TetO-PM
Plasmid#197387PurposeTo establish all-in-one TetO-inducible cell line for Pdx1-Mafa expression (Doxycycline induced; rtTA included; Blasticidin resistant)DepositorInsertPDX1-T2A-MAFA
UseLentiviral; TetoTagsExpressionMammalianMutationPromoterTREAvailable sinceFeb. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
mouse av full length
Plasmid#217809PurposeFull length mouse integrin av expressionDepositorInsertITGAV (Itgav Mouse)
UseTagsP2A-EGFPExpressionMammalianMutationPromoterCAGAvailable sinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDA056
Plasmid#131387PurposeORFeus expression, WTDepositorInsertORFeus-Hs
UseTagsExpressionMammalianMutationPromoterTRE3GAvailable sinceJune 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
hZMPSTE24-3FLAG_pQCXIP
Plasmid#89759PurposeHuman ZMPSTE24 mammalian expression vector. Retroviral bicistronic puromycin vector.DepositorInsertHomo sapiens zinc metallopeptidase STE24 (ZMPSTE24) (ZMPSTE24 Human)
UseRetroviral; Bicistronic expression: puromycin re…Tags9bp spacer (NotI site) - 3xFLAG epitopes - Stop c…ExpressionMammalianMutationY253H in ZMPSTE24 (see depositor comments below)PromoterCMVAvailable sinceJune 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-hA3D-BE3
Plasmid#113413PurposeExpresses hA3D-BE3 in mammalian cellsDepositorInserthA3D-BE3 (APOBEC3D Human, S. pyogenes and Bacteriophage PBS2)
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
PLKO-mcherry-luc-puro-Renilla cDNA
Plasmid#29783DepositorInsertRenilla
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 1, 2011AvailabilityAcademic Institutions and Nonprofits only -
pSH231-Bx-GFP-C31
Plasmid#115149PurposeEmpty safe harbor site 231 knock-in vector with flanking homology arms and Bxb1 and C31 attP integrase landing padsDepositorInsertSafe Harbor Homology Sequences
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOG-Cre
Plasmid#233265PurposePlasmid expressing Cre recombinase that is used to insert DNA CARGO into RMCE homing locus in Rosa26DepositorInsertCre recombinase
UseCre/LoxTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDA095
Plasmid#131391Purposegeneration of Tet-On RPEDepositorInsertORFeus
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
plexm-human LRRC33
Plasmid#111600PurposeExpress human LRRC33 in 393T cellsDepositorInsertLRRC33 (NRROS Human)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceJune 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTF023_His_hsDDA1
Plasmid#174480PurposeExpresses DDA1 in insect cellsDepositorInsertDDA1 (DDA1 Human)
UseTagsHis-TEVExpressionInsectMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTV-Nanog_2-5prime-1000-24MS96T-3F_NeoR
Plasmid#174882PurposeTargeting vector for generating Nanog STREAMING-tag KI cellDepositorInsertSTREAMING-tag with homology arms for Nanog
UseMouse TargetingTagsExpressionMutationPromoterAvailable sinceDec. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBSKΔBS-SD_MCS_SA(NBSI)-24xMS2-sirius-96xopto-TetO-Int-3xFLAG-NeoR-GS4
Plasmid#177265PurposeSTREAMING-tag cassetteDepositorInsertSTREAMING-tag cassette
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceDec. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENTR223-EBNA2
Plasmid#195815PurposeGateway Entry Clone of Epstein-Barr virus EBNA2DepositorInsertEBNA2 (EBNA-2 Epstein-Barr virus)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceMay 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-EGFP-P2A-FusionRed
Plasmid#191108PurposeMammalian cell line expression plasmids for production of EGFP and FusionRed proteinsDepositorInsertpAAV-CAG-EGFP-P2A-FusionRed
UseAAVTagsFusionRedExpressionMammalianMutationSee depositor comments belowPromoterchicken β-actin promoterAvailable sinceNov. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDC-UAS-nBitbow1.0(miniW)
Plasmid#138329PurposeDrosophila attB-targeting plasmid that contains UAS driving nBitbow1.0 cassette. Expresses combinations of nuclear mAmetrine, mTFP, mNeonGree, mKusabira-Orange2, and mKate2 upon Flp recombination.DepositorInsertsmAmetrine
tdKatushka2
mNeonGreen
mTFP1
mKusabira-Orange2
UseTagshuman H2BExpressionInsectMutationPromoter10xUASAvailable sinceMarch 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pB[U6-3 wex2 PUb-DsRed]
Plasmid#169010PurposeFor germline transformation. Expresses white exon 2 sgRNA and DsRed marker in all cells.DepositorInsertwhite ex2 sgRNA
UsePiggybacTagsExpressionInsectMutationPromoterDrosophila melanogaster U6:3Available sinceApril 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-V5-PTPN14-wild type
Plasmid#61003PurposeExpresses wild type human PTPN14 in mammalian cells, N-terminus V5 tagDepositorInsertPTPN14 (PTPN14 Human)
UseTagsV5 tagExpressionMammalianMutationPromoterCMVAvailable sinceNov. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDule2-Tet2.0
Plasmid#85497PurposePlasmid for incorporating the non-canonical amino acid Tetrazine 2.0 with the Mj Tet2.0 synthetase and cognate amber suppressing tRNA in Ecoli.DepositorInsertTetrazine2.0 tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
UseTagsExpressionBacterialMutationY32G L65Q F108S Q109D D158S L162NPromoterlpp (constitutive)Available sinceJan. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
1255_pAAV-U6-SA-BbsI-MluI-gRNA-CB-SACas9-HA-OLLAS-spA
Plasmid#109320PurposePlasmid for AAV SaCas9 Mammalian Expression with a BbsI cloning site for a gRNADepositorTypeEmpty backboneUseAAV and CRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJune 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
011LP-pAAV-TNT4-EmGFP-pA
Plasmid#120280PurposeAAV vector for cardiomyocyte specific expression of Emerald Green Fluorescent Protein using cardiac troponin T promoterDepositorInsertEmGFP
UseAAVTags3XFlag and HAExpressionMutationPromoterTNT Cardiac Troponin T promoterAvailable sinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLYS1-MICU1-Flag
Plasmid#50058PurposeExpression of human MICU1 with Flag tag in mammalian cellsDepositorInsertMICU1 (isoform 2) (MICU1 Human)
UseTagsFlagExpressionMammalianMutation*PromoterAvailable sinceMay 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
pENTR223-EBNA3C
Plasmid#198576PurposeGateway Entry CloneDepositorInsertEBNA3C (EBNA-3B/EBNA-3C Epstein-Barr virus)
UseGateway entry vectorTagsExpressionMutationNone (wildtype)PromoterAvailable sinceMay 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-anti-VEGF(Nb35) PAGER(TF)
Plasmid#230000PurposeExpresses anti-VEGF PAGER(TF) in mammalian cells; used with NanoLuc-Arrestin-TEVp (Addgene #125228) and UAS-Firefly Luciferase reporter (Addgene #104840)DepositorInsertIL2SP-Aro6-Nb35-TEVcs-ALFA-KORD(RAA)-LOV-TEVcs-Gal4
UseAAVTagsExpressionMammalianMutationV360A/R361A on KORDPromoterCMVAvailable sinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-anti-TNFalpha(Ozoralizumab) PAGER(TF)
Plasmid#230001PurposeExpresses anti-TNFalpha PAGER(TF) in mammalian cells; used with NanoLuc-Arrestin-TEVp (Addgene #125228) and UAS-Firefly Luciferase reporter (Addgene #104840)DepositorInsertIL2SP-Aro6-Ozoralizumab-TEVcs-ALFA-KORD(RAA)-LOV-TEVcs-Gal4
UseAAVTagsExpressionMammalianMutationV360A/R361A on KORDPromoterCMVAvailable sinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-anti-CCL2(8E10) PAGER(TF)
Plasmid#230002PurposeExpresses anti-CCL2 PAGER(TF) in mammalian cells; used with NanoLuc-Arrestin-TEVp (Addgene #125228) and UAS-Firefly Luciferase reporter (Addgene #104840)DepositorInsertIL2SP-Aro6-8E10-TEVcs-ALFA-KORD(RAA)-LOV-TEVcs-Gal4
UseAAVTagsExpressionMammalianMutationV360A/R361A on KORDPromoterCMVAvailable sinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-anti-mCherry(LaM8) PAGER(TF)
Plasmid#229999PurposeExpresses anti-mCherry PAGER(TF) (high reversibility) in mammalian cells; used with NanoLuc-Arrestin-TEVp (Addgene #125228) and UAS-Firefly Luciferase reporter (Addgene #104840)DepositorInsertIL2SP-Aro6-LaM8-TEVcs-ALFA-KORD(RAA)-LOV-TEVcs-Gal4
UseAAVTagsExpressionMammalianMutationV360A/R361A on KORDPromoterCMVAvailable sinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-anti-mCherry(LaM6) PAGER(TF)
Plasmid#229998PurposeExpresses anti-mCherry PAGER(TF) (high sensitivity) in mammalian cells; used with NanoLuc-Arrestin-TEVp (Addgene #125228) and UAS-Firefly Luciferase reporter (Addgene #104840)DepositorInsertIL2SP-Aro6-LaM6-TEVcs-ALFA-KORD(RAA)-LOV-TEVcs-Gal4
UseAAVTagsExpressionMammalianMutationV360A/R361A on KORDPromoterCMVAvailable sinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJS646
Plasmid#183175PurposeY2H vector compatible with pJS647 PYR1 insertDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceAug. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMXs-Sig10-Fc-IG
Plasmid#183722PurposeExpresses the fusion protein containing the extracellular part of human Siglec-10 and the Fc region of human IgG1, and GFPDepositorInsertSiglec-10
UseRetroviralTagsFc of human IgG1ExpressionMutationPromoterAvailable sinceJune 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDule-Tet2.0
Plasmid#85496PurposePlasmid for incorporating the non-canonical amino acid Tetrazine 2.0 with the Mj Tet2.0 synthetase and cognate amber suppressing tRNA in Ecoli.DepositorInsertTetrazine2.0 tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
UseTagsNoneExpressionBacterialMutationY32G L65Q F108S Q109D D158S L162NPromoterlpp (constitutive)Available sinceJan. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAV-CAG-Sun1-GFP-WPRE-BC2-pA
Plasmid#220754PurposeExpression of Sun1GFP for nuclei isolation and Projection-TAG BC for multiplex projection tracingDepositorInsertSun1-GFP-WPRE-BC2
UseAAVTagsExpressionMutationPromoterCAGAvailable sinceJuly 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAV-CAG-Sun1-GFP-WPRE-BC1-pA
Plasmid#220753PurposeExpression of Sun1GFP for nuclei isolation and Projection-TAG BC for multiplex projection tracingDepositorInsertSun1-GFP-WPRE-BC1
UseAAVTagsExpressionMutationPromoterCAGAvailable sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pENTR223-EBNA3B
Plasmid#198574PurposeGateway Entry CloneDepositorInsertEBNA3B (EBNA-3B/EBNA-3C Epstein-Barr virus)
UseGateway entry vectorTagsExpressionMutationNone (wildtype)PromoterAvailable sinceMay 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENTR223-EBNA3A
Plasmid#198575PurposeGateway Entry CloneDepositorInsertEBNA3A (EBNA-3A Epstein-Barr virus)
UseGateway entry vectorTagsExpressionMutationNone (wildtype)PromoterAvailable sinceMay 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
mouse a2b full length
Plasmid#217812PurposeFull length mouse integrin a2b expressionDepositorInsertITGA2B (Itga2b Mouse)
UseTagsP2A-EGFPExpressionMammalianMutationPromoterCAGAvailable sinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
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NDUFV3_pcDNA6.2/EmGFP-Bsd
Plasmid#176946PurposeMammalian expression vector encoding NDUFV3 and EmGFP-BsdDepositorInsertNDUFV3 (NDUFV3 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
MSP469
Plasmid#65771PurposeHuman expression vector for SpCas9 VQR variant: CMV-T7-humanSpCas9(D1135V/R1335Q/T1337R)-NLS-3xFLAG (VQR variant)DepositorInsertmammalian codon-optimized Streptococcus pyogenes Cas9 (D1135V/R1335Q/T1337R)-NLS-3XFlag
UseCRISPRTags3x FLAG and NLSExpressionMammalianMutationD1135V, R1335Q, and T1337R mutations in Cas9PromoterCMV & T7Available sinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDA007
Plasmid#131380PurposeORFeus expression, WTDepositorInsertORFeus-Hs
UseTagsORF2-3xFlagExpressionMammalianMutationPromoterAvailable sinceJune 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ166-OsPE2
Plasmid#141080PurposeGateway entry clone for CRISPR-Cas9(H840A) prime editingDepositorInsertzCas9(H840A)-OsM-MLV-RT
UseCRISPR; Gateway compatible zcas9(h840a)-osm-mlv-rtTags3x FLAG, NLS and NLSExpressionPlantMutationPromoterAvailable sinceMay 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR223-BRLF1(Rta)
Plasmid#195824PurposeGateway Entry Clone of Epstein-Barr virus BRLF1DepositorInsertBRLF1 (BRLF1 Epstein-Barr virus)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceMay 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
MSP680
Plasmid#65772PurposeHuman expression vector for SpCas9 EQR variant: CMV-T7-humanSpCas9(D1135E/R1335Q/T1337R)-NLS-3xFLAG (EQR variant)DepositorInsertmammalian codon-optimized Streptococcus pyogenes Cas9 (D1135E/R1335Q/T1337R)-NLS-3XFlag
UseCRISPRTags3x FLAG and NLSExpressionMammalianMutationD1135E, R1335Q, and T1337R mutations in Cas9PromoterCMV & T7Available sinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCG-TPMV Hd32-IL-13
Plasmid#163869PurposeExpresses TPMV H glycoprotein bearing IL-13 in mammalian cellsDepositorInsertTPMV H glycoprotein/IL-13
UseTagsHuman IL-13ExpressionMammalianMutationAmino acids 2 to 33 at N terminus deletedPromoterAvailable sinceMay 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
BPK2101
Plasmid#65770PurposeBacterial expression plasmid for S. aureus Cas9 & sgRNA (need to clone in spacer into BsaI sites): T7-humanSaCas9-NLS-3xFLAG-T7-BsaIcassette-Sa-sgRNADepositorInsertmammalian codon-optimized SaCas9, and SaCas9 gRNA
UseCRISPRTagsNLS-3xFlagExpressionBacterialMutationPromoterT7 (x2)Available sinceJune 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn1-EGFP-P2A-EGFPf-WPRE-HGHpA
Plasmid#74513PurposeAAV vector that use human synapsin-1 promoter to drive the expression of EGFP and membrane-targeted EGFPf linked by self-cleaving P2A peptide.DepositorInsertEGFP-p2A-EGFP-f
UseAAVTagsExpressionMutationPromoterAvailable sinceJuly 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaM-3
Plasmid#172769PurposeBacterial expression of anti-mCherry nanobody LaM-3, with pelB leader and C-term free cysteine and 6xHIS tag.DepositorInsertLaM-3
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only
