We narrowed to 9,391 results for: Coli
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Plasmid#182404PurposeBacterial expression of Vesicle Nucleating peptide6-stefinA fusionDepositorInsertVNp6-stefin (CSTA Human)
UseTagsVesicle Nucleating peptide 6 (VNp6)ExpressionBacterialMutationPromoterT7Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
MBP-MEK1 S222TAG
Plasmid#68302PurposeN-terminal MBP tagged human MEK1 gene S222TAG for bacterial expressionDepositorInsertMBP-MEK1 S222TAG (MAP2K1 Human, Synthetic)
UseTagsHis and MBPExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_VNp-FGF21
Plasmid#182407PurposeBacterial expression of Vesicle Nucleating peptide-fgf21 fusionDepositorInsertVNp-fgf21 (FGF21 Human)
UseTagsVesicle Nucleating peptide (VNp)ExpressionBacterialMutationPromoterT7Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
R26TV LSL-TRL
Plasmid#68476PurposeRosa26 targeting vector expressing Cre-dependent tTR-KRAB-rtTA3-Luc cassetteDepositorInserttTR-KRAB-2A-rtTA3-2A-Luciferase
UseCre/Lox, Luciferase, and Mouse TargetingTagsExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
MBP-MEK1 S218TAG
Plasmid#68301PurposeN-terminal MBP tagged human MEK1 gene S218TAG for bacterial expressionDepositorInsertMBP-MEK1 S218TAG (MAP2K1 Human, Synthetic)
UseTagsHis and MBPExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBXNPHM3-Nb_MsbA#1
Plasmid#186428PurposePlasmid for bacterial expression of MsbA binding Nanobody Nb_MsbA#1DepositorInsertNanobody Nb_MsbA#1
UseAffinity Reagent/ AntibodyTags3C cleavage site, His Tag, Maltose Binding Protei…ExpressionMutationPromoterpBADAvailable sinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSBK.013
Plasmid#187213PurposeExpresses retron Eco1 ncRNA v35 (long a1/a2, barcode 5 [ACG-CGT]), and Cas1 + Cas2DepositorInsertsRetron Eco1 ncRNA v35 (long a1/a2, barcode 5 [ACG-CGT])
Cas1 + Cas2
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterT7/LacAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xEcoLeuT(CUA)_EF1_AnapRS
Plasmid#174894PurposeAnapRS expression for amber suppression in mammalian cells; transient or piggy bac mediated integrationDepositorInsertAnapRS
UseTagsExpressionMammalianMutationL38F, M40G, L41P, Y499V, Y500L, Y527A, H537E, L53…PromoterEF1Available sinceNov. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBEST-p70a-UTR3-Nterm 6xHis mRFP-T500
Plasmid#92317PurposeExpression of NTerm 6xHis tagged mRFP using the p70a promoter and UTR3DepositorInsertOptimized NTerm 6xHis tagged monomeric RFP (mRFP)
UseSynthetic Biology; Cell free protein synthesis (c…Tags6xHisExpressionBacterialMutationPromoterp70aAvailable sinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCIBN-tagBFP-TetR
Plasmid#103797PurposeBLInCR 'Localizer' construct that marks tetO arrays and is targeted by a PHR-tagged effector upon illumination with blue lightDepositorUseTagsExpressionMammalianMutationTetR: A4G (M2V)PromoterCMVAvailable sinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV iGluf
Plasmid#105833PurposeFast indicator for extracellular glutamate imagingDepositorInsertiGluSnFR E25D variant
UseTagsMyc and PDGFRExpressionMammalianMutationChanged Glu 25 to AspPromoterCMVAvailable sinceJune 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_VNp-StefinA
Plasmid#182403PurposeBacterial expression of Vesicle Nucleating peptide-stefinA fusionDepositorInsertVNp-stefin (CSTA Human)
UseTagsVesicle Nucleating peptide (VNp)ExpressionBacterialMutationPromoterT7Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSBK.014
Plasmid#187215PurposeExpresses retron Eco1 ncRNA v35 (long a1/a2, barcode 6 [CAG-CTG]), and Cas1 + Cas2DepositorInsertsRetron Eco1 ncRNA v35 (long a1/a2, barcode 6 [CAG-CTG])
Cas1 + Cas2
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterT7/LacAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPPC001
Plasmid#171138PurposeFor integration of Sp.pCas9-dCas9 and BBa_J23107-MCP-SoxS(R93A/S101A) with miniTn7T methodDepositorInsertSp.pCas9-dCas9_BBa_J23107-MCP-SoxS(R93A, S101A)
UseCRISPRTagsExpressionBacterialMutationSoxS has R93A and S101A mutationsPromoterSp.pCas9, BBa_J23107Available sinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSBK.009
Plasmid#187209PurposeExpresses retron Eco1 ncRNA v35 (long a1/a2, barcode 1 [CCT-AGG]), and Cas1 + Cas2DepositorInsertsRetron Eco1 ncRNA v35 (long a1/a2, barcode 1 [CCT-AGG])
Cas1 + Cas2
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterT7/LacAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDN-G1Tt
Plasmid#44509DepositorInsertsUseSynthetic Biology; Expression regulator/reporterTagsExpressionYeastMutationTwo tandem tet operators (2XtetO2) downstream of …PromoterPGal1-D12 promoterAvailable sinceApril 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pZJQIBEBT003
Plasmid#140053PurposeThe pZJQIBEBT003 plasmid contains the coding sequence of eMutS-L157C-G233C gene which has the ability of error removal in the gene synthesis process.DepositorInserteMutS-L157C-G233C-CBM-EGFP
UseTagsHis-TagExpressionBacterialMutationL157C, G233CPromoterT7Available sinceJune 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBC2T-Ypet-FtnA
Plasmid#108969Purposeexpresses BC2-tag-Ypet-FtnA in mammalian cellsDepositorInsertFtnA (ftnA Escherichia coli)
UseTagsBC2-tag and YpetExpressionMammalianMutationPromoterAvailable sinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNIC28-BSA4-G-S18
Plasmid#127825PurposeProduction of recombinant human ribosomal protein S18DepositorInsertRPS18 (RPS18 Human)
UseTagsExpressionBacterialMutationPromoterT7Available sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pT7-agrBD-IV
Plasmid#53440PurposeDerivative of pT7-agrBD-I with a point mutation in the agrD gene to change AIP coding region from type-I to type-IVDepositorInsertagrBD locus (with D29Y mutation in agrD)
UseSynthetic BiologyTagsV5 epitope, 6x HIS (not expressed on agrB or agrD…ExpressionBacterialMutationD to Y mutation of residue 29 of the agrD gene (c…PromoterT7-lacAvailable sinceJune 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBEST-p70a-UTR1-Nterm 6xHis PL-T500
Plasmid#92314PurposeExpression of NTerm 6xHis tagged protein L (PL) domain using the p70a promoter and UTR1DepositorInsertB1 domain of protein L (PL)
UseSynthetic Biology; Cell free protein synthesis (c…Tags6xHisExpressionBacterialMutationPromoterp70aAvailable sinceOct. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBB0323-TEV-His12
Plasmid#137039Purposeexpression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi BB0323 with C-terminal TEV-protease-cleavable His12DepositorInsertAAC66700.1
UseTagsHis12 (TEV protease cleavable)ExpressionBacterialMutationwild type, full-length protein, contains signal p…PromoterT7Available sinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCIBN-EYFP-TetR
Plasmid#103798PurposeBLInCR 'Localizer' construct that marks tetO arrays and is targeted by a PHR-tagged effector upon illumination with blue lightDepositorUseTagsExpressionMammalianMutationTetR: A4G (M2V)PromoterCMVAvailable sinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBEST-p70b-UTR1-deGFP-6xHis-T500
Plasmid#92222PurposeExpression of deGFP using the p70b promoter (weak version of the p70a promoter)DepositorInsertdeGFP
UseSynthetic Biology; Cell free protein synthesis (c…Tags6xHisExpressionBacterialMutationPromoterp70b (p70a promoter with one mutation)Available sinceOct. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
p1585588
Plasmid#62620PurposeePathBrick pETM6 vector encoding CsF3H, CsDFR, and DuLAR (1:3:3 Ratio)DepositorInsertsflavaone 3B-hydroxylase
dihydroflavonol 4-reductase
leucoanthocyanidin reductase
dihydroflavonol 4-reductase
dihydroflavonol 4-reductase
leucoanthocyanidin reductase
leucoanthocyanidin reductase
UseSynthetic BiologyTagsGBD, PDZ, and SH3ExpressionBacterialMutationPromoterT7Available sinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
WT MetNI in pBAD
Plasmid#118254PurposeWild Type Methionine ABC Transporter MetNI in pBAD Vector for Transport Activity AssayDepositorUseTags10x His Tag and Enterokinase Cleavage SiteExpressionBacterialMutationPromoterArabinose Promoter and NoneAvailable sinceNov. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMM549
Plasmid#112531PurposepZA1D-hrtR-chuA - HrtR expressed consitutively from promoter ProD with RBS2, ChuA expressed consitiutively from promoter J23107, p15a origin, AmpRDepositorInsertsHrtR
ChuA
UseTagsExpressionBacterialMutationPromoterJ23107 and ProDAvailable sinceFeb. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
R0063 (pLuxR-pR)_AB
Plasmid#66012PurposeMoClo Basic Part: Controllable promoter - pLuxR(pR) - luxR regulated (right promoter, weak constitutive down regulated by LuxR, C0062 in combination with homoserine lactone (HSL)) [A:R0063:B]DepositorInsertControllable promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBEST-p70a-UTR1-Nterm 6xHis mRFP-T500
Plasmid#92316PurposeExpression of NTerm 6xHis tagged mRFP using the p70a promoter and UTR1DepositorInsertOptimized NTerm 6xHis tagged monomeric RFP (mRFP)
UseSynthetic Biology; Cell free protein synthesis (c…Tags6xHisExpressionBacterialMutationPromoterp70aAvailable sinceAug. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pZS1[FruR-T]
Plasmid#60752PurposeContains PIq driving expression FruR-T, the Fructose inducible chimera with the TAN DBD.DepositorInsertsFruR-T
FruR-T
UseSynthetic BiologyTagsExpressionBacterialMutationChimeric LacI/GalR repressor with the TAN DBD and…PromoterAvailable sinceApril 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
hSRAP.HMG6.wt.Dlink.a1-236
Plasmid#49813Purposeexpression vector for MBP-SRAP(1-236) fusionDepositorInsertsteroid receptor RNA activator protein (SRA1 Human)
UseTagsE. coli Maltose Binding ProteinExpressionBacterialMutationwildtype codon-optimized for E. coli expressionPromoterT7Available sinceJan. 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pPB758
Plasmid#210033PurposeContains inducible Cas7-P2A-Cas5-T2A-Cas8, rtTA-T2A-puroDepositorInsertsCas7-P2A-Cas5-T2A-Cas8
rtta-T2A-puro
UseCRISPRTagsSV40 NLSExpressionMammalianMutationPromoterEF-1α and TRE3GAvailable sinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
p202-Syt1-C2A-wt
Plasmid#201553PurposeExpresses wild-type Octopus synaptotagmin-1 C2A domain in E. coliDepositorInsertSynaptotagmin-1 C2A domain (partial)
UseTagsExpressionBacterialMutationCodon-optimized for E. coliPromoterRBSAvailable sinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJM919_WT His POLI
Plasmid#201444PurposeLow copy vector for expressing human DNA polymerase iota in E. coli with an N-terminal 6x His tagDepositorInsertDNA polymerase iota (POLI Human)
UseTags6x HisExpressionBacterialMutationCoding sequence has been optimised for expression…PromoterlacIqAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET32a-hNRG1a
Plasmid#199235PurposeBacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.DepositorInsertNeuregulin (NRG1 Human)
UseTagsTrxA-6xHis-S-tagExpressionBacterialMutationPromoterT7Available sinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSCL.178
Plasmid#184999PurposeExpress -Eco1 FANCF editing ncRNA and gRNADepositorInsertEco1: FANCF targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter
UseTagsSV40NLSExpressionMammalianMutationFANCF donor, a1/a2 length extended for 27 bpPromoterH1Available sinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSCL.176
Plasmid#184997PurposeExpress -Eco1 RNF2 editing ncRNA and gRNADepositorInsertEco1: RNF2 targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter
UseTagsSV40NLSExpressionMammalianMutationRNF2 donor, a1/a2 length extended for 27 bpPromoterH1Available sinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSCL.179
Plasmid#185000PurposeExpress -Eco1 HEK4 editing ncRNA and gRNADepositorInsertEco1: HEK4 targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter
UseTagsSV40NLSExpressionMammalianMutationHEK4 donor, a1/a2 length extended for 27 bpPromoterH1Available sinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
hU6-sgNeo1 (opti)
Plasmid#177240PurposeSame as pLL3.3;U6(BstXI)-XhoI-chimeric RNA;PGK-Cre but XhoI stuffer removed, expresses neomycin gRNADepositorInsertsgNeo1
UseLentiviralTagsExpressionMutationPromoterhU6Available sinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
hU6-sgNeo2 (opti)
Plasmid#177241PurposeSame as pLL3.3;U6(BstXI)-XhoI-chimeric RNA;PGK-Cre but XhoI stuffer removed, expresses neomycin gRNADepositorInsertsgNeo2
UseLentiviralTagsExpressionMutationPromoterhU6Available sinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only