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166,169 results

Showing: 4601 - 4640 of 166169 results

  1. pSBbi-Pur

    Plasmid
    #60523
    Purpose
    SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: puromycin resistance gene
    Depositor
    Eric Kowarz
    Article
    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b
    Type
    Empty backbone
    Use
    Transposon
    Expression
    Mammalian
    Promoter
    EF1a/RPBSA
    Available Since
    Feb. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    60523-plasmid-map-sequence-id-305758

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  3. pCK302

    Plasmid
    #87768
    Purpose
    As pCK301 (E. coli rhaBAD promoter upstream of sfGFP, sfGFP can be replaced with any gene of interest), but rhaS cloned downstream of ampR, which allows non-metabolisable inducer L-mannose to be used
    Depositor
    John Heap
    Article
    Kelly et al ACS Synth Biol. 2016 Oct 21;5(10):1136-1
    Inserts
    PrhaBAD-sfGFP
    rhaS from E. coli with its native RBS from E. coli
    Use
    Synthetic Biology
    Tags
    6xHis Tag
    Expression
    Bacterial
    Promoter
    PrhaBAD rhamnose-inducible promoter from E. coli …
    Available Since
    May 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    87768-plasmid-map-sequence-id-171131

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  6. pLEX307-APN-puro

    Plasmid
    #158454
    Purpose
    Constitutive lentiviral expression of human APN.
    Depositor
    Alejandro Chavez, Sho Iketani
    Insert
    APN (ANPEP Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Available Since
    Aug. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    158454-plasmid-map-sequence-id-308145

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  7. HiBiT-SRPK1 Fusion Vector

    Plasmid
    #236908
    Purpose
    Express HiBiT-SRPK1 in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    SRPK1 (SRPK1 Human)
    Tags
    HiBiT
    Expression
    Mammalian
    Mutation
    None
    Promoter
    CMV
    Available Since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    236908-plasmid-map-sequence-id-473390

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  9. BM00654

    Plasmid
    #155339
    Purpose
    Binary vector for expression of PULSE system (under the control of CaMV35S promoter) controlling FLuc. It constitutively expresses RLuc and a plant selection cassette.
    Depositor
    Matias Zurbriggen
    Article
    Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi
    Insert
    RB_KanR_Tnos-NLS-PIF6-E-P35S_Tnos-RLuc-PUbi10_Tnos-NLS-VP16-PhyB-P35S_Tnos-EL222-NLS-SRDX-P35S_T35S-FLuc-POpto_LB
    Use
    Luciferase and Synthetic Biology
    Expression
    Plant
    Available Since
    Oct. 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    155339-plasmid-map-sequence-id-360426

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  15. pBa-LSS-GFP-LDLR wt

    Plasmid
    #98184
    Purpose
    Low Density Lipoprotein Receptor N-terminally tagged with Green Fluorescent Protein. LSS= LDLR signal sequence, which is cleaved leaving the GFP attached to the mature LDLR protien.
    Depositor
    Gary Banker, Marvin Bentley
    Article
    Jenkins et al J Cell Biol. 2012 Aug 20;198(4):749-61.
    Insert
    LDLR (LDLR Human)
    Tags
    GFP and LDLR signal sequence, N-term of GFP so GF…
    Expression
    Mammalian
    Mutation
    none
    Promoter
    Chicken Beta Actin
    Available Since
    Sept. 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    98184-plasmid-map-sequence-id-490370

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  17. pEGFP-C1-TULP3

    Plasmid
    #218741
    Purpose
    Expresses C-terminally EGFP-tagged TULP3 in mammalian cells
    Depositor
    Kazuhisa Nakayama
    Insert
    TULP3 (TULP3 Human)
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    May 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    218741-plasmid-map-sequence-id-434240

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  21. Bidirectional MYC 5'UTR Dual Fluorescence Reporter

    Plasmid
    #229498
    Purpose
    This is a lentiviral reporter for translation initiation under the control of the Myc 5'UTR, which produces destabilized GFP (dsGFP). There is a control destabilized mCherry (dsmCherry) as a control.
    Depositor
    Davide Ruggero
    Article
    Kovalski et al Nat Cell Biol. 2025 Feb 4. doi: 10.1038/
    Insert
    MYC 5'Untranslated region + destabilized GFP
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    Ef1a
    Available Since
    Feb. 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    229498-plasmid-map-sequence-id-456853

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  32. pCMV-T7-SpRY-P2A-EGFP (RTW4830)

    Plasmid
    #139989
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpRY(A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized SpCas9 variant named SpRY with BPNLS-3xFLAG-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…
    Promoter
    CMV and T7
    Available Since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    139989-plasmid-map-sequence-id-275392

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  34. TFORF2409

    Plasmid
    #144021
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    FOXF2 (FOXF2 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Feb. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    144021-plasmid-map-sequence-id-385816

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  36. CAG-GFP/cre

    Plasmid
    #49054
    Purpose
    Retroviral vector contains CAG promoter , CMV enhancer and a large synthetic intron that used to allow the ubiquitous expression of GFP-fused Cre recombinase
    Depositor
    Fred Gage
    Article
    Tashiro et al Nat Protoc. 2006;1(6):3049-55.
    Insert
    GFP/Cre
    Use
    Retroviral
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Nov. 4, 2013
    Availability
    Academic Institutions and Nonprofits only
    49054-plasmid-map-sequence-id-490793

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  38. mEGFP-TPX2-N-10

    Plasmid
    #56289
    Purpose
    Localization: Centrosomes, Excitation: 488, Emission: 507
    Depositor
    Michael Davidson
    Insert
    TPX2 (TPX2 Human)
    Tags
    mEGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    March 4, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 4601 - 4640 of 166169 results