We narrowed to 9,586 results for: Coli
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Plasmid#86204PurposeExpresses efflux pump in E. coli to provide resistance to biofuel (low copy)DepositorInsertEfflux pump
PromoterATCGTTTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATAA…Available SinceMarch 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMAL-c5X-Ad I
Plasmid#66998PurposeExpresses malE-Ad I in E. coliDepositorInsertmalE-Ad I
TagsHis6 and malE-Factor XaExpressionBacterialPromotertacAvailable SinceJan. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMAL-c5X-SPR16
Plasmid#67001PurposeExpresses malE-SPR16 in E. coliDepositorInsertmalE-SPR16
TagsHis6 and malE-Factor XaExpressionBacterialPromotertacAvailable SinceJan. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMAL-c5X-poly(alanylglycine)
Plasmid#67003PurposeExpresses malE-poly(alanylglycine) in E. coliDepositorInsertmalE-poly(alanylglycine)
TagsHis6 and malE-Factor XaExpressionBacterialPromotertacAvailable SinceJan. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMAL-c5X-AB12
Plasmid#67005PurposeExpresses malE-AB12 in E. coliDepositorInsertmalE-AB12
TagsHis6 and malE-Factor XaExpressionBacterialPromotertacAvailable SinceJan. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMAL-c5X-BQ_6
Plasmid#67006PurposeExpresses malE-BQ_6 in E. coliDepositorInsertmalE-BQ_6
TagsHis6 and malE-Factor XaExpressionBacterialPromotertacAvailable SinceJan. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMAL-c5X-poly-EAK9
Plasmid#67010PurposeExpresses malE-poly-EAK9 in E. coliDepositorInsertmalE-poly-EAK9
TagsHis6 and malE-Factor XaExpressionBacterialPromotertacAvailable SinceJan. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHL 886
Plasmid#64907PurposeKanR + ColE1 + PLlacO-1::RBS(st2):lacZ::RBS(st7):cfp::T1 terminatorDepositorTypeEmpty backboneUseSynthetic Biology; E. coliExpressionBacterialAvailable SinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET His10-Draper INT
Plasmid#126670PurposeExpression of His10-Draper intracellular domain for purification from E. coliDepositorInsertDraper cytoplasmic domain
ExpressionBacterialAvailabilityAcademic Institutions and Nonprofits only -
pAKW.031-GM-R5
Plasmid#154044PurposeExpresses R5 peptide in E. coliDepositorInsertSilaffin R5 peptide
TagsMaltose binding protein and his tagExpressionBacterialPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pAAV-syn-FLP-FRT-iGluSnFR4s-NGR-WPRE
Plasmid#234453PurposeAAV-mediated expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow); NGR vector matches or outperforms PDGFR in all conditions tested.DepositorInsertiGluSnFR4s-NGR
UseAAV; Flp/frtTagsIgK chain and Myc epi tag-NGR GPI anchorExpressionMammalianPromoterSynapsinAvailable SinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET32bplus_trx_dAChE4
Plasmid#83917PurposeEncodes an E. coli codon optimized version of dAChE4 - a designed variant of human acetylcholinesterase bearing 51 mutations with ~1800 fold higher functional expression levels in E. coliDepositorInsertDesigned hAChE, codon optimised for E.coli expression (ACHE Synthetic, Human)
TagsHis, S-tag, and TRXExpressionBacterialMutationV13T,K24T,M43V,L49R,V61W,S68N,E92N,T110K,S111N,T1…PromoterT7 promoterAvailable SinceMarch 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
piggyBac_rtTA (4th_Gen)_Gateway_IRES_NGN2-2A-PURO_Synapsin-BirA. (pEHA1618)
Plasmid#209084PurposeNgn2 mediated cortical neuron induction, gateway destination, Synapsin promoter BirA ligase expressionDepositorAvailable SinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-syn-FLP-FRT-iGluSnFR4f-NGR-WPRE
Plasmid#234454PurposeAAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested.DepositorInsertiGluSnFR4f-NGR
UseAAV; Flp/frtTagsIgK chain and Myc epi tag-NGR GPI anchorExpressionMammalianPromoterSynapsinAvailable SinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pK27Sumo_His-SUMO-nsp10-nsp16 fusion (SARS-CoV-2)
Plasmid#169191PurposeTo express nsp10-nsp16 fusion protein in E. coliDepositorInsert14His-SUMO-nsp10-GSGSGS-nsp16 (ORF1ab Synthetic, SARS-CoV-2)
Tags14His-SUMO and Fusion protein of SARS-CoV-2 nsp10…ExpressionBacterialMutationCodon optimised for E. coliPromoterT5Available SinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLXI_TRC403 LacZ
Plasmid#111184PurposeControl inducible vector with LacZ (V5 tagged)DepositorInsertlacZ
UseLentiviralTagsV5PromoterTREAvailable SinceMarch 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLXI_TRC401 LacZ
Plasmid#111183PurposeControl inducible vector with LacZ (V5 tagged)DepositorInsertlacZ
UseLentiviralTagsV5PromoterTREAvailable SinceMarch 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA_FRT_SNCA-Avi_HygR-2A-BirA.dna(TO_deleted). (pEHA1448)
Plasmid#209083PurposeSNCA (Avi tagged) expression along with BirA ligaseDepositorInsertsTagsAviExpressionMammalianMutationhuman codon optimizedPromoterCMVAvailable SinceOct. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXPR_BRD003 Luciferase
Plasmid#117072Purposesingle guide RNA targeting LuciferaseDepositorInsertLuciferase
UseCRISPRPromoterhU6Available SinceMarch 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
HemH-R212A_pET-14b
Plasmid#233262PurposeOverexpress his-tag HemH protein with a site directed mutagenesis, arginine at position 212 was substituted with alanine (R121A)DepositorInsertHemH (hemH )
ExpressionBacterialMutationArginine was replaced with Alanine at position 21…PromoterT7 promoterAvailable SinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDZ2087
Plasmid#92414PurposeExpresses His-tagged mutant (L56V/S135G/S219V) TEV protease in E. coliDepositorInsertTEV protease
TagsMBP (self cleaving)ExpressionBacterialMutationL56V/S135G/S219VPromotertacAvailable SinceJuly 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pIMAY
Plasmid#68939PurposeE. coli/staphylococcal temperature-sensitive plasmid for allelic exchangeDepositorTypeEmpty backboneUseStaphylococcal allelic exchange vectorAvailable SinceSept. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-GFP(0)
Plasmid#199166Purposeused to express a neutral GFP variant in E. coliDepositorInsertGFP(0)
TagsMGHHHHHHGGAMutationE16R, I138R, D207R, N222K, L246RPromoterT7Available SinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGS-21a his6-GST-WTPhi29DNAP
Plasmid#166863PurposeTo produce a dual affinity-tagged Phi29 DNA polymeraseDepositorInsertPhi29 Polymerase codon-optimized for overexpression in E. coli
TagsGST and his6ExpressionBacterialAvailable SinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet1_CylLL_CylM
Plasmid#208759PurposeExpresses modified cytolysin L (mCylLL) in E. coliDepositorInsertsCylLL
CylLM
TagsHisx6 TagExpressionBacterialPromoterT7Available SinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAGTetRnls
Plasmid#26599DepositorAvailable SinceNov. 15, 2010AvailabilityAcademic Institutions and Nonprofits only -
pET303_sumocodo_GFP_codoopti
Plasmid#210393PurposeExpress SUMO-GFP in E. coliDepositorInsertGFP
TagsSUMO-tagExpressionBacterialAvailable SinceJan. 13, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pL99
Plasmid#78577PurposeOver-expression shuttle vector for gene cloning and transfer from E. coli to Streptomyces. Contains inducible PnitA-NitR systemDepositorTypeEmpty backboneUseStreptomyces expressionExpressionBacterialPromoterinducible PnitA-NitR systemAvailable SinceMay 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28 MBP_PrA/G-H6
Plasmid#176077PurposeExpress His6 tagged MBP_PrA/G in Escherichia coliDepositorInsertMBP PrA/G-H6
TagsHis tagExpressionBacterialPromoterT7 PromoterAvailable SinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-UnaG
Plasmid#203485PurposeFor recombinant protein production in Escherichia coli. His-tagged UnaG under the control of the T7 promoter.DepositorInsertUnaG
ExpressionBacterial and PlantAvailable SinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pL97
Plasmid#78576PurposeOver-expression shuttle vector for gene cloning and transfer from E. coli to Streptomyces. Contains strong constitutive ermEp promoterDepositorTypeEmpty backboneUseStreptomyces expressionExpressionBacterialPromoterermEp* promoter from S. erythraeaAvailable SinceApril 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET N6his GFP1-9
Plasmid#182240PurposeTripartite split-GFP. E. coli vector. Expression of GFP1-9 detector fragment.DepositorInsertGFP1-9
Tags6HISExpressionBacterialPromoterT7Available SinceApril 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pG1AK-sfGFP
Plasmid#71739PurposeModular shuttle vector for Geobacillus and E. coliDepositorInsertsfGFP
UseSynthetic Biology; Shuttle vectorExpressionBacterialPromoterpRplsWTAvailable SinceJuly 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T1 mouse PARG (439-959)
Plasmid#132615Purposeexpresses mouse PARG(439-959) in E. coli.DepositorInsertPARG (439-959)
ExpressionBacterialAvailable SinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSB1C3-J23110-B0034-mRFP1_Yellow
Plasmid#160443PurposeBioBrick pSB1C3 plasmid that constitutively overexpresses mRFP1_Yellow chromoprotein in E. coliDepositorInsertpromoter, RBS, mRFP1E_Yellow
UseSynthetic BiologyMutationBioBrick sites removedAvailable SinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRSETA_crGE
Plasmid#172927PurposeCrowding FRET sensor with mCerulean3 and mCitrine for E. coli expression. Linker comprises 2 helices and 3 GSG domains.DepositorInsertCrowdingSensorGE
TagsHisExpressionBacterialPromoterT7Available SinceAug. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pML433
Plasmid#177190PurposeExpresses recombinant HyPro enzyme in E. coliDepositorInsertHyPro enzyme
ExpressionBacterialPromoterT7Available SinceNov. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
Pet28a-His-tag-BS2
Plasmid#220347PurposeE. coli expression vector to purify BS2DepositorInsertBS2
TagsHis tagExpressionBacterialAvailable SinceJune 18, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMOD4 galK-GT
Plasmid#19183DepositorInsertgalK (galK E.coli)
UseRecombineeringTags50 nucleotides from N-term TAP, C-term TAP, and F…Available SinceJan. 29, 2009AvailabilityAcademic Institutions and Nonprofits only -
pND004
Plasmid#200950PurposeMBP-tagged E. coli protein expression destination vector for Golden Gate cloningDepositorTypeEmpty backboneTagsMBPExpressionBacterialPromoterT7Available SinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSF-T20S
Plasmid#110805Purposeexpresses Thermoplasma acidophilium 20S proteasome in E. ColiDepositorInsertsPsmB
PsmA
Tagstev-HisExpressionBacterialMutationN2D and Q2EPromoterT7 and T7 promoterAvailable SinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSK+KanaRpsL
Plasmid#20871DepositorInsertKanamycinResistance-RpsL
ExpressionBacterialMutationnoneAvailable SinceMay 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
pJBM4
Plasmid#225291PurposeE. coli-Campylobacter shuttle vector; TetRDepositorInsertTet(O) and its putative promoter
ExpressionBacterialAvailable SinceOct. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pALACE
Plasmid#31853DepositorTypeEmpty backboneUseEscherichia coli-mycobacterium shuttle plasmidTags6xHISExpressionBacterialPromoterAcetamidase operoneAvailable SinceJuly 9, 2012AvailabilityAcademic Institutions and Nonprofits only -
pRSF-KpAGO207-1251
Plasmid#117854PurposeExpressed KpAGO 207-1251 in E. coli under IPTG-inducible promotorDepositorInsertArgonaute
Tags6xHis-SUMO encoded in vectorExpressionBacterialMutationmissing first 206 amino acid residuesPromoterT7Available SinceOct. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZA16mflon
Plasmid#75439PurposePlasmid for arabinose-induced expression of mf-lon. Note that this plasmid contains the original mf-lon cassette that is not codon optimized for expression in E. coli.DepositorInsertmf-lon
ExpressionBacterialPromoterpBADAvailable SinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSAP
Plasmid#206429PurposeA vector backbone domesticated for SapI (pCC1BAC-based), contains elements for selection, maintenance, and propagation in S. cerevisiae (TRP1) and E. coli (cat).DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSG15-pBAD-tetO-deGFP
Plasmid#102450PurposeExpresses deGFP fluorescent protein in E. coli. The expression is controlled by a pBAD-tetO combinatorial promoter.DepositorInsertdeGFP
ExpressionBacterialPromoterpBAD-tetOAvailable SinceOct. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEcoFus
Plasmid#187833Purpose1st generation E. coli - Fusobacterium nucleatum shuttle vector for overexpressionDepositorTypeEmpty backboneExpressionBacterialAvailable SinceNov. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIII/MS2-1
Plasmid#220629PurposeYeast/E. coli shuttle vector in which an RNA polymerase III promoter directs transcription of an RNA containing two tandem MS2 sites.DepositorTypeEmpty backboneExpressionYeastAvailable SinceOct. 9, 2024AvailabilityAcademic Institutions and Nonprofits only