We narrowed to 9,406 results for: Coli
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Plasmid#227222PurposeExpresses human USP7 with a N-terminal cleavable 6His tag in E. coliDepositorInsertUSP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
UseTags6HisExpressionBacterialMutationV485GPromoterT7-promoterAvailable SinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-a(+)-6xHis-mScarlet-I(+5)
Plasmid#219418PurposeFluorescent protein to reconstruct the mixture that mimics the charge profile of E. coli lysateDepositorInsertmScarlet-I(+5)
UseTagsMGHHHHHGGExpressionBacterialMutationD125K, D186K, E216K, E238KPromoterT7Available SinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-LflChmG
Plasmid#221343PurposeExpress LflChmG in E. coliDepositorInsertchmG
UseTagsHis6ExpressionBacterialMutationPromoterAvailable SinceJuly 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-LflChmH
Plasmid#221344PurposeExpress LflChmH in E. coliDepositorInsertchmH
UseTagsHis6ExpressionBacterialMutationPromoterAvailable SinceJuly 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-ChmF
Plasmid#221345PurposeExpress ChmF in E. coliDepositorInsertchmF
UseTagsHis6ExpressionBacterialMutationPromoterAvailable SinceJuly 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-ChmK
Plasmid#221346PurposeExpress ChmK in E. coliDepositorInsertchmK
UseTagsHis6ExpressionBacterialMutationPromoterAvailable SinceJuly 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-ChmL
Plasmid#221347PurposeExpress ChmL in E. coliDepositorInsertchmL
UseTagsHis6ExpressionBacterialMutationPromoterAvailable SinceJuly 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETDuet-1-chmI
Plasmid#221341PurposeExpress ChmI in E. coliDepositorInsertchmI
UseTagsHis6ExpressionBacterialMutationPromoterAvailable SinceJuly 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-ChmJ
Plasmid#221342PurposeExpress ChmJ in E. coliDepositorInsertchmJ
UseTagsHis6ExpressionBacterialMutationPromoterAvailable SinceJuly 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-H12TEV-hEIF5B-587~1220
Plasmid#208637Purposefor E. coli expression truncated human eIF5B (ENST00000289371), aa587~1220DepositorInserthuman eIF5B (ENST00000289371) aa 587~1220 (EIF5B Human)
UseTags6xhisExpressionBacterialMutationcontain SNP rs1376443, protein sequence not chang…PromoterT7Available SinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-mCherry(-6)-L1
Plasmid#205046PurposeFluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coliDepositorInsertmCherry(-6)-L1
UseTagsMGHHHHHGGExpressionBacterialMutationPromoterAvailable SinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-mCherry(-6)-L2
Plasmid#205047PurposeFluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coliDepositorInsertmCherry(-6)-L2
UseTagsMGHHHHHGGExpressionBacterialMutationPromoterAvailable SinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-mCherry(-6)-L3
Plasmid#205048PurposeFluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coliDepositorInsertmCherry(-6)-L3
UseTagsMGHHHHHGGExpressionBacterialMutationPromoterAvailable SinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-GFP(-6)-L1
Plasmid#205027PurposeFluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coliDepositorInsertGFP(-6)-L1
UseTagsKGRRGKKSRK and MGHHHHHGGAExpressionBacterialMutationPromoterAvailable SinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-GFP(-6)-L2
Plasmid#205028PurposeFluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coliDepositorInsertGFP(-6)-L2
UseTagsKGRRGKKSRKGRRGKKSRK and MGHHHHHGGAExpressionBacterialMutationPromoterAvailable SinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-GFP(-6)-L3
Plasmid#205029PurposeFluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coliDepositorInsertGFP(-6)-L3
UseTagsKGRRGKKSRKGRRGKKSRKGRRGKKSRK and MGHHHHHGGAExpressionBacterialMutationPromoterAvailable SinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-GFP(-6)-H1
Plasmid#205030PurposeFluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coliDepositorInsertGFP(-6)-H1
UseTagsKKRRRKK and MGHHHHHGGAExpressionBacterialMutationPromoterAvailable SinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-GFP(-6)-H2
Plasmid#205031PurposeFluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coliDepositorInsertGFP(-6)-H2
UseTagsKKRRRKKKRRRKK and MGHHHHHGGAExpressionBacterialMutationPromoterAvailable SinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-GFP(-6)-H3
Plasmid#205032PurposeFluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coliDepositorInsertGFP(-6)-H3
UseTagsKKRRRKKKRRRKKKRRRKK and MGHHHHHGGAExpressionBacterialMutationPromoterAvailable SinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-RPL3-Δ1~34,Δ215~284-TwStrep
Plasmid#208630Purposefor E. coli expression of truncated human RPL3 (ENST00000216146), aa35~398 with deletion of 215~284, fused with Twin Strep tag.DepositorInserthuman RPL3 (ENST00000216146) Δ1~34, Δ215~284 (RPL3 Human)
UseTags6xHis, Twin Strep, and mEGFP(A206K)ExpressionBacterialMutationΔ1~34,Δ215~284PromoterT7Available SinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTatABmNeonC
Plasmid#178464PurposepTatABC with an mNeonGreen fluorescent protein tag at the C-terminal end of TatB.DepositorInsertTatA, TatBmNeon, TatC
UseTagsmNeonGreen fused to TatBExpressionBacterialMutationPromoteraraCAvailable SinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTatABCmNeon
Plasmid#178465PurposepTatABC with an mNeonGreen fluorescent protein tag at the C-terminal end of TatC.DepositorInsertTatA, TatB, TatCmNeon
UseTagsmNeonGreen fused to TatCExpressionBacterialMutationPromoteraraCAvailable SinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pE5772
Plasmid#170779Purposeexpression of hybrid proteins in E. coliDepositorInsertGentR
UseTagspolyhisbioMbpTEVExpressionBacterialMutationPromoterT7Available SinceOct. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSecEffector wo TEVp
Plasmid#172892Purposeatc inducible (in any E. coli strain) BxbI and arabinose inducible TP901DepositorInsertsBxb1
TP901
UseTagsExpressionBacterialMutationPromoternTETO and pBAD /AraCAvailable SinceJuly 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDest-894 (attR4-attR2)
Plasmid#161893PurposeMultisite Gateway (attR4-attR2) vector for expression of multiple proteins in E. coliDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable SinceJan. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEPMY1CB0001
Plasmid#162317PurposeAcceptor for golden gate assembly with BsaI overhangs GCTT-CCAT. Generates plasmids for T7-driven expression in E. coli. Contains mRFP1 reporter.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable SinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEPQD0KN0025
Plasmid#162283PurposeAcceptor for golden gate assembly with BsaI overhangs GCTT-AATG. Generates plasmids for T7-driven E. coli cell-free protein synthesisDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable SinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSUI-SynP-CAS1sg
Plasmid#154341PurposeExpression of the sgRNA targeting to the E. coli can geneDepositorInsertCAS1 sgRNA
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterAvailable SinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSUI-SynP-CAS2sg
Plasmid#154342PurposeExpression of the sgRNA targeting to the E. coli can geneDepositorInsertCAS2 sgRNA
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterAvailable SinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSUI-SynP-CANTsg
Plasmid#154343PurposeExpression of the sgRNA targeting to the E. coli can geneDepositorInsertCANT sgRNA
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterAvailable SinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSUI-SynP-CAPsg
Plasmid#154344PurposeExpression of the sgRNA targeting to the E. coli can geneDepositorInsertCAP sgRNA
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterAvailable SinceJuly 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SznF E215A
Plasmid#139565Purposeexpresses SznF E215A in E. coliDepositorInsertSznF E215A
UseTagshexahistidineExpressionBacterialMutationchanged glutamate 215 to alaninePromoterT7Available SinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SznF
Plasmid#139564Purposeexpresses SznF in E. coliDepositorInsertSznF
UseTagsExpressionBacterialMutationPromoterT7Available SinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAW092
Plasmid#113250PurposeE. coli expression vector for His-MBP-S. pyogenes Cas1 H205ADepositorInsertS. pyogenes Cas1
UseTags6xHis-MBPExpressionBacterialMutationH205APromoterT7Available SinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBbA5k-AcrB/AcrD/AcrFa, ABO_0964
Plasmid#86202PurposeExpresses efflux pump in E. coli to provide resistance to biofuel (medium copy)DepositorInsertEfflux pump
UseTagsExpressionBacterialMutationPromoterATCGTTTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATAA…Available SinceMarch 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHL 309
Plasmid#64906PurposeKanR + ColE1 + PLlacO-1::RBS(st7):rfp::RBS(st7):cfp::RBS(st7):yfp::T1 terminatorDepositorTypeEmpty backboneUseSynthetic Biology; E. coliTagsExpressionBacterialMutationPromoterAvailable SinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHL 1988
Plasmid#64908PurposeKanR + ColE1 + PLlacO-1::RBS(st7):cfp::Asp terminator + PLlacO-1::RBS(st7):psu::T1 terminatorDepositorTypeEmpty backboneUseSynthetic Biology; E. coliTagsExpressionBacterialMutationPromoterAvailable SinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCVD453
Plasmid#66154Purposecloning of E. coli E2348/69 left end of the LEE region, contains rorf1 and espG.DepositorInsertLEE probe A rorf1, espG
UseCloning vectorTagsExpressionMutationPromoternoneAvailable SinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJT104
Plasmid#25088DepositorInsertEdge detector delta luxI
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable SinceFeb. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
enz.043-SETD7-Y305F
Plasmid#154045PurposeExpresses SETD7 (Y305F) in E. coliDepositorInsertHistone-lysine N-methyltransferase SETD7 (SETD7 Human)
UseTagsHis, MBPExpressionBacterialMutationchanged tyrosine 305 to phenylalaninePromoterT7Available SinceAvailabilityAcademic Institutions and Nonprofits only -
pAKW.119-R5-TEV-SUMO-His
Plasmid#154050PurposeExpresses R5 peptide in E. coliDepositorInsertSilaffin R5 peptide
UseTagsSUMO and His tagExpressionBacterialMutationPromoterT5LacOAvailable SinceAvailabilityAcademic Institutions and Nonprofits only -
pAKW.111A-SETD7
Plasmid#154052PurposeExpresses SETD7 (Y305F) in E. coliDepositorInsertHistone-lysine N-methyltransferase SETD7 (SETD7 Human)
UseTagsExpressionBacterialMutationchanged tyrosine 305 to phenylalaninePromoterpTet*Available SinceAvailabilityAcademic Institutions and Nonprofits only -
pETNb-cALFA
Plasmid#159989PurposeFor the protein expression with ALFA tag at C-terminus in E. coliDepositorTypeEmpty backboneUseTagsHRV3C protease cleavage site-ALFA tag-8histidineExpressionBacterialMutationPromoterAvailable SinceDec. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRPF185
Plasmid#106367PurposeC. difficile inducible expression system. Shuttle plasmid containing a tetracycline-inducible gusADepositorInsertTerm(fdx)-Ptet-gusA-Term(slpA) (gusA )
UseE. coli - c. difficile shuttle vectorTagsExpressionMutationcodon optimised for C. difficilePromoterPtetAvailable SinceMarch 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET15b-AavLEA1
Plasmid#53093PurposeAavLEA1 expression (in BL21 DE3 E. coli strain)DepositorInsertAavLEA1
UseTags6xHisExpressionBacterialMutationPromoterT7Available SinceJune 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-GFP-Nanobody
Plasmid#61838PurposeExpresses GST-tagged anti-GFP Nanobody in E. coli cells.DepositorInsertGFP-Nanobody
UseTagsGSTExpressionBacterialMutationPromoterAvailable SinceMay 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET24b-APEX2
Plasmid#111702PurposeFor IPTG-inducible expression of APEX2 in E. coliDepositorInsertAPEX2
UseTags6xHis and V5ExpressionBacterialMutationPromoterT7 promoterAvailable SinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJK225
Plasmid#71124PurposeExpression of Acetobacter aceti 1023 citrate synthase (AaCSH6) in E. coliDepositorInsertcitrate synthase
UseTagsHis6ExpressionBacterialMutationPromoterT7Available SinceJan. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28a-sfGFP 150TAG
Plasmid#85493PurposepET28a expressing E. coli codon optimized superfolder GFP with TAG stop codon at position 150DepositorInsertsuperfolder GFP 150TAG
UseTagsExpressionBacterialMutation150 TAG (amino acid 150 mutated to stop codon)PromoterT7Available SinceJan. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pACλCI-β-flap (831-1057)
Plasmid#53733PurposeExpression of λCI fused via three alanines to E. coli RNAP beta subunit, aa 831-1057 (β-flap). Can be cut with Not1 and BamHI to replace β-flap with a moiety of interest.DepositorInsertλCI-β-flap fusion
UseTagsExpressionBacterialMutationPromoterlac UV5Available SinceJune 4, 2014AvailabilityAcademic Institutions and Nonprofits only