We narrowed to 7,507 results for: PAC

1. pSF-pMB1'- trc-YFP

   Plasmid

   #186565

   Purpose

   pMB1' ori, lacI, Ptrc promoter, AmpR. Kringle YFP (Yellow Fluorescence Protein) expression

   Depositor

   Teresa de Diego Puente

   Article

   Lozano Terol et al Front Microbiol. 2021 Jun 21;12:682001.

   Insert

   YFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   trc promoter

   Available since

   Nov. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSF-pMB1'- tac-YFP

   Plasmid

   #186566

   Purpose

   pMB1' ori, lacI, Ptac promoter, AmpR. Kringle YFP (Yellow Fluorescence Protein) expression

   Depositor

   Teresa de Diego Puente

   Article

   Lozano Terol et al Front Microbiol. 2021 Jun 21;12:682001.

   Insert

   YFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   tac promoter

   Available since

   Nov. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pYPQ141-ZmUBI-RZ-Cas12j2

   Plasmid

   #189781

   Purpose

   Cas12j2 (CasΦ) Gateway gRNA entry plasmid using Zea mays Ubi promoter and HH, HDV ribozyme processing

   Depositor

   Yiping Qi

   Article

   Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

   Insert

   gRNA cloning site
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET21a(+)_co_L-HicDH

   Plasmid

   #185453

   Purpose

   CDS of L-HicDH codon optimized for expression in Synechocystis sp. PCC 6803 in pET21a(+).

   Depositor

   Wolfgang Kroutil

   Article

   Jurkas et al Eng Microbiol. 2021 Nov 26;2(1):100008.

   Insert

   L-HicDH from Lactobacillus confusus DSM 20196
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUE1-TSP-ORF68 K174A/R179A/K182A

   Plasmid

   #162654

   Purpose

   Expresses ORF68 K174A/R179A/K182A in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage site

   Depositor

   Britt Glaunsinger

   Article

   Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

   Insert

   ORF68
   

   Use

   Tags

   N-terminal 2xStrep-Tag II and HRV 3C site

   Expression

   Mammalian

   Mutation

   Amino acids K174, R179, and K182 mutated to alani…

   Promoter

   CMV

   Available since

   Feb. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAT-9251

   Plasmid

   #124226

   Purpose

   Bacterial SpCas9-HF1 expression

   Depositor

   Ervin Welker

   Article

   Talas et al Nucleic Acids Res. 2021 Jan 15. pii: 610

   Insert

   SpCas9-HF1
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   TetR/TetA

   Available since

   Feb. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pFastBac-LacI-LAP-HEC1

   Plasmid

   #114033

   Purpose

   Production of baculovirus with LacI-LAP-HEC1

   Depositor

   Geert Kops

   Article

   Hiruma et al Science. 2015 Jun 12;348(6240):1264-7. d

   Insert

   LacI-LAP-HEC1 (NDC80 Human)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   none

   Promoter

   CMV

   Available since

   Aug. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pX459V2.0-eSpCas9(1.1)+K855A

   Plasmid

   #108298

   Purpose

   pX459 V2.0 (Plasmid #62988) with the K848A, K855A, K1003A, and R1060A mutations

   Depositor

   Yuichiro Miyaoka

   Article

   Kato-Inui et al Nucleic Acids Res. 2018 May 18;46(9):467

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pX459V2.0-eSpCas9(1.1)+K810A

   Plasmid

   #108297

   Purpose

   pX459 V2.0 (Plasmid #62988) with the K810A, K848A, K1003A, and R1060A mutations

   Depositor

   Yuichiro Miyaoka

   Article

   Kato-Inui et al Nucleic Acids Res. 2018 May 18;46(9):467

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pX459V2.0-eSpCas9(1.1)+K810A+K855A

    Plasmid

    #108299

    Purpose

    pX459 V2.0 (Plasmid #62988) with the K810A, K848A, K855A, K1003A, and R1060A mutations

    Depositor

    Yuichiro Miyaoka

    Article

    Kato-Inui et al Nucleic Acids Res. 2018 May 18;46(9):467

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pEGFPN1-ATG9A

    Plasmid

    #198529

    Purpose

    Expression of ATG9A-GFP fusion protein in mammalian cells

    Depositor

    Juan Bonifacino

    Article

    Mattera et al Proc Natl Acad Sci U S A. 2017 Dec 12;11

    Insert

    ATG9A (ATG9A Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    silent mutations in codons 4 and 775

    Promoter

    CMV

    Available since

    March 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCMV-eNme2-C-ABE8e

    Plasmid

    #185667

    Purpose

    Expresses eNme2-C-ABE8e in mammalian cells

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

    Insert

    ecTadA(8e)-neNme2-C
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Nme2Cas9 P6S/D16A/E33G/K104T/D152A/F260L/A263T/A3…

    Promoter

    Available since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. CMV-Nme2ABE8e

    Plasmid

    #189928

    Purpose

    Plasmid encoding Nme2ABE8e driven by the CMV promoter for plasmid transfection.

    Depositor

    David Liu

    Article

    Davis et al Nat Biomed Eng. 2022 Jul 28. pii: 10.103

    Insert

    Nme2ABE8e
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Nme2Cas9 D16A

    Promoter

    CMV

    Available since

    Sept. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCiGSΔΨ

    Plasmid

    #108312

    Purpose

    Foamy virus helper plasmid expressing Gag, driven by a CMV promoter. Ampicillin resistant.

    Depositor

    David Russell

    Article

    Josephson et al Hum Gene Ther. 2004 Jan;15(1):87-92.

    Insert

    gag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    pCiGSΔΨ is a newer version of the pCiGS Gag expre…

    Promoter

    CMV

    Available since

    June 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCMV5 BRGI-Flag

    Plasmid

    #19143

    Depositor

    Joan Massague

    Article

    Xi et al J Biol Chem. 2008 Jan 11. 283(2):1146-55

    Insert

    SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (SMARCA4 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 3, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. IFN-Beta_pGL3

    Plasmid

    #102597

    Purpose

    IFN-beta promoter driving luciferase. Use in IFN reporter assay.

    Depositor

    Nicolas Manel

    Article

    Gentili et al Science. 2015 Sep 11;349(6253):1232-6. d

    Insert

    Human IFN-Beta promoter (IFNB1 Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLdCN

    Plasmid

    #84290

    Purpose

    Express gRNA and Cas9 in Leishmania with Neomycin resistance

    Depositor

    Greg Matlashewski

    Article

    Zhang et al mSphere. 2017 Jan 18;2(1). pii: e00340-1

    Insert

    gRNA and Cas9
    

    Use

    CRISPR; Leishmania

    Tags

    Expression

    Mutation

    Promoter

    L. donovani ribosome RNA promoter

    Available since

    Jan. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA5-FRT-TO-Nsp16-HF

    Plasmid

    #157725

    Purpose

    mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp16 under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    Nsp16-HF (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    His8-Flag

    Expression

    Mammalian

    Mutation

    codon optimized

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pcDNA5-FRT-TO-Nsp16

    Plasmid

    #157723

    Purpose

    mammalian expression of untagged SARS-CoV-2 Nsp16 under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    Nsp16 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    codon optimized

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pENN236_CRISPRcharm_Kv2

    Plasmid

    #220839

    Purpose

    CRISPRcharm Kv2 expression in mammalian cells

    Depositor

    Jonathan Weissman

    Article

    Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

    Insert

    CRISPRcharm Kv2
    

    Use

    CRISPR

    Tags

    P2A-TagBFP

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    CAG

    Available since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pHEN-CoV2-nanobody-F1

    Plasmid

    #194592

    Purpose

    Bacterial expression of F1 nanobody targeting the receptor binding domain (RBD) of SARS-CoV-2 spike

    Depositor

    Gerald McInerney

    Article

    Hanke et al Sci Adv. 2022 Mar 25;8(12):eabm0220. doi

    Insert

    F1 (S )
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pHEN-CoV2-nanobody-E2

    Plasmid

    #194590

    Purpose

    Bacterial expression of E2 nanobody targeting the receptor binding domain (RBD) of SARS-CoV-2 spike

    Depositor

    Gerald McInerney

    Article

    Hanke et al Sci Adv. 2022 Mar 25;8(12):eabm0220. doi

    Insert

    E2 (S )
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pHEN-CoV2-nanobody-G6

    Plasmid

    #194589

    Purpose

    Bacterial expression of G6 nanobody targeting the receptor binding domain (RBD) of SARS-CoV-2 spike

    Depositor

    Gerald McInerney

    Article

    Hanke et al Sci Adv. 2022 Mar 25;8(12):eabm0220. doi

    Insert

    G6 (S )
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pHEN-CoV2-nanobody-C7

    Plasmid

    #194591

    Purpose

    Bacterial expression of C7 nanobody targeting the receptor binding domain (RBD) of SARS-CoV-2 spike

    Depositor

    Gerald McInerney

    Article

    Hanke et al Sci Adv. 2022 Mar 25;8(12):eabm0220. doi

    Insert

    C7 (S )
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCas3cRh

    Plasmid

    #133773

    Purpose

    Expresses all necessary components of Type I-C CRISPR-Cas system

    Depositor

    Joseph Bondy-Denomy

    Article

    Csorgo et al Nat Methods. 2020 Oct 19. pii: 10.1038/s

    Inserts

    RhaR (rhaR )
    RhaS (rhaS )
    pRhaBAD
    I-C crRNA
    Cas3
    Cas5
    Cas8
    Cas7
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pENN239_CRISPRcharm_Kv1

    Plasmid

    #220838

    Purpose

    CRISPRcharm Kv1 expression in mammalian cells

    Depositor

    Jonathan Weissman

    Article

    Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

    Insert

    CRISPRcharm Kv1
    

    Use

    CRISPR

    Tags

    P2A-TagBFP

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    CAG

    Available since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pTRIP-CMV-Puro-2A-cGAS

    Plasmid

    #102612

    Purpose

    Lentivector expressing cGAS

    Depositor

    Nicolas Manel

    Article

    Gentili et al Science. 2015 Sep 11;349(6253):1232-6. d

    Insert

    cGAS (CGAS Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCMV-PE6e

    Plasmid

    #207855

    Purpose

    Mammalian expression of PE6e prime editor

    Depositor

    David Liu

    Article

    Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

    Insert

    PE6e
    

    Use

    Tags

    SV40 bpNLS and SV40 bpNLS, c-Myc NLS

    Expression

    Mammalian

    Mutation

    Cas9(H840A, K918A, K775R)

    Promoter

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pRSFDuet-1_VNp-DNAseI

    Plasmid

    #182409

    Purpose

    Bacterial expression of Vesicle Nucleating peptide-DNaseI fusion

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Insert

    VNp-DNase I (DNASE1 Bovine)
    

    Use

    Tags

    Vesicle Nucleating peptide (VNp)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA Flag Lamp2b-HA

    Plasmid

    #71292

    Purpose

    Flag tag with 3 residue spacer fused to the N-terminus of Lamp2b, a transmembrane exosomal protein. 3 residue spacer then HA tag fused to the C-terminus of Lamp2b.

    Depositor

    Joshua Leonard

    Article

    Hung et al J Biol Chem. 2015 Mar 27;290(13):8166-72

    Insert

    Lamp2b (LAMP2 Human)
    

    Use

    Tags

    Flag, HA, and Lamp2 signal peptide

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. phCMV-coGALVenv-C4070A

    Plasmid

    #172217

    Purpose

    Expresses the envelope protein of Gibbon Ape Leukemia Virus (GALV) for pseudotyping of retroviral or lentiviral vectors

    Depositor

    Boris Fehse

    Article

    Mirow et al Viruses. 2021 Jul 27;13(8). pii: v130814

    Insert

    Envelope protein of Gibbon Ape Leukemia Virus (codon-optimized version)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Carries C-terminus of amphotropic MLV strain 4070…

    Promoter

    CMV immediate early promoter

    Available since

    Aug. 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pRubiG-T2A-Cas9

    Plasmid

    #75348

    Purpose

    Ubiquitin promoter expresses GFP and humanized spCas9 (PX330) via a T2A motif. For cell transfection or use in retroviral (MMuLV) packaging. Use Pac1 and/or BstB1 sites to insert sgRNAs from PXL.

    Depositor

    Bryan Luikart

    Article

    Williams et al Sci Rep. 2016 May 10;6:25611. doi: 10.10

    Insert

    Cas9
    

    Use

    CRISPR and Retroviral

    Tags

    GFP (via t2a)

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    May 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCMV-PEmaxRNaseHtrunc

    Plasmid

    #207858

    Purpose

    Mammalian expression of PEmaxDRNaseH prime editor

    Depositor

    David Liu

    Article

    Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

    Insert

    PEmaxDRNaseH
    

    Use

    Tags

    SV40 bpNLS and SV40 bpNLS, c-Myc NLS

    Expression

    Mammalian

    Mutation

    M-MLVRTDRNAseHrt

    Promoter

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. ABE8.20-m

    Plasmid

    #136300

    Purpose

    expresses ABE8.20-m in mammalian cells

    Depositor

    Nicole Gaudelli

    Article

    Gaudelli et al Nat Biotechnol. 2020 Apr 13. pii: 10.103

    Insert

    ABE8.20-m
    

    Use

    Tags

    C-terminal BPNLS

    Expression

    Mammalian

    Mutation

    D10A in S. pyogenes Cas9, TadA mutations describe…

    Promoter

    Available since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCMV-ABE7.10

    Plasmid

    #102919

    Purpose

    expresses ABE7.10 in mammalian cells

    Depositor

    David Liu

    Article

    Gaudelli et al Nature. 2017 Nov 23;551(7681):464-471. d

    Insert

    ABE7.10
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    see manuscript

    Promoter

    CMV

    Available since

    Nov. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pRSFDuet-1_VNp-mNeongreen-Etanercept

    Plasmid

    #182421

    Purpose

    Bacterial expression of Vesicle Nucleating peptide-mNeongreen-Etanercept fusion

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Insert

    VNp-mNeongreen-Etanercept (TNFRSF1B Human)
    

    Use

    Tags

    Vesicle Nucleating peptide (VNp) & mNeongreen

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pSicoR-eIF1a-GFP-RPL10a

    Plasmid

    #159890

    Purpose

    Expresses GFP-RPL10a for ribosomal immunoprecipitation

    Depositor

    James Ellis

    Article

    Rodrigues et al Cell Rep. 2020 Mar 24;30(12):4179-4196.e

    Insert

    RPL10a (RPL10A Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mutation

    Promoter

    eIF1a

    Available since

    Sept. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pTP261

    Plasmid

    #104166

    Purpose

    Bacterial expression plasmid of anti-GFP nanobody with 1x Cysteine for maleimide labeling

    Depositor

    Dirk Görlich

    Article

    Pleiner et al Elife. 2015 Dec 3;4:e11349. doi: 10.7554

    Insert

    Anti-GFP nanobody Enhancer PDB 3K1K (1x Cysteine)
    

    Use

    Tags

    14x Histidine tag and NEDD8 from Brachypodium dis…

    Expression

    Bacterial

    Mutation

    1x Cysteine located at bps 493-495 in full sequen…

    Promoter

    Available since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. GFP-Rab27B

    Plasmid

    #89447

    Purpose

    Expression of GFP-tagged human Rab27B in mammalian cells

    Depositor

    Wendy Westbroek

    Article

    Hendrix et al J Natl Cancer Inst. 2010 Jun 16;102(12):

    Insert

    human Rab27B (RAB27B Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    CMV

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. NES-EGFP-cPHx3

    Plasmid

    #116855

    Purpose

    PI(3,4)P2 biosensor

    Depositor

    Gerry Hammond

    Article

    Goulden et al J Cell Biol. 2019 Mar 4;218(3):1066-1079

    Insert

    PLEKHA1 (PLEKHA1 Human)
    

    Use

    Tags

    EGFP and X.leavis map2k1.L(32-44)

    Expression

    Mammalian

    Mutation

    amino acids 169-329 (tandem trimer)

    Promoter

    Available since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge