161,407 results

1. pHAGE-TO-dCas9-GFP

   Plasmid

   #64106

   Purpose

   Expression of Sp dCas9-GFP in mammalian cells

   Depositor

   Thoru Pederson

   Article

   Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

   Insert

   Sp dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   sfGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-TO

   Available since

   May 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYZ960

   Plasmid

   #187972

   Purpose

   TEF1p-Cas9(noBsmBI)-CYC1t and tRNA-GFP-SUP4t with 2µ-URA3

   Depositor

   Jef Boeke

   Article

   Zhao et al Nucleic Acids Res. 2023 Jun 16:gkad491.

   Insert

   Cas9 (codon optimized for human)
   

   Use

   Synthetic Biology

   Tags

   SV40

   Expression

   Yeast

   Mutation

   Promoter

   TEF1

   Available since

   Sept. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pmRFP-LC3

   Plasmid

   #21075

   Purpose

   Mammalian expression of rat LC3 fused to mRFP

   Depositor

   Tamotsu Yoshimori

   Article

   Kimura et al Autophagy. 2007;3(5):452-60.

   Insert

   microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
   

   Use

   Tags

   mRFP

   Expression

   Mammalian

   Mutation

   First ATG is not included.

   Promoter

   Available since

   May 28, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFGAMS-mCherry

   Plasmid

   #166024

   Purpose

   Expresses human PFAS-mCherry fusion in mammalian cells

   Depositor

   Stephen Benkovic

   Article

   Doigneaux et al J Biol Chem. 2020 Jul 10;295(28):9551-95

   Insert

   PFAS-mCherry (PFAS Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti6-AMPK alpha1 (1-312)

   Plasmid

   #162131

   Purpose

   Expression vector for constitutively active AMPK alpha 1

   Depositor

   Boyi Gan

   Article

   Liu et al Nat Cell Biol. 2016 Apr;18(4):431-42. do

   Insert

   AMPK alpha1 (1-312) (PRKAA1 Rat)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mutation

   Promoter

   Available since

   Feb. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. GCN5 core-dCas9-p300 core

   Plasmid

   #179553

   Purpose

   encodes S. pyogenes dCas9 with n-terminal fusion of human GCN5 core (aa 473-676) and c-terminal fusion of human p300 core (aa 1048-1664) driven by EF-1alpha promoter

   Depositor

   Isaac Hilton

   Article

   Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78

   Insert

   S. pyogenes dCas9 with n-terminal fusion of human GCN5 core (aa 473-676) and c-terminal fusion of human p300 core (aa 1048-1664) (EP300, KAT2A Human)
   

   Use

   Lentiviral

   Tags

   Flag Tag

   Expression

   Mammalian

   Mutation

   D10A; H840A in S.pyogenes Cas9

   Promoter

   EF-1alpha

   Available since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. Actin-cpstFRET-Actin

   Plasmid

   #80643

   Purpose

   cpstFRET actin sensor for mechanical force measurements in cytoskeleton

   Depositor

   Fred Sachs

   Article

   Guo et al Proc Natl Acad Sci U S A. 2014 Dec 9;111

   Insert

   Beta actin, cpstFRET
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. YIplac211-ABP1-iGFPx6

   Plasmid

   #177099

   Purpose

   Expresses Abp1-iGFPx6 in yeast cells

   Depositor

   Benjamin Glick

   Article

   Casler et al J Cell Biol. 2022 Jan 3;221(1). pii: 212

   Insert

   ABP1 (ABP1 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pF CAG luc hygro

   Plasmid

   #67502

   Purpose

   Constitutive expression of firefly luciferase in mammalian cells

   Depositor

   Brett Stringer

   Article

   Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

   Insert

   Firefly luciferase
   

   Use

   Lentiviral and Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Feb. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. MSCV-mNINJ1-GFP

    Plasmid

    #208776

    Purpose

    MSCV vector expressing WT mouse NINJ1

    Depositor

    Petr Broz

    Article

    Ramos et al EMBO J. 2024 Apr;43(7):1164-1186. doi: 1

    Insert

    NINJ1 (Ninj1 Mouse)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Available since

    Aug. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti-tetON-KRAB-dCas9-DHFR-EF1a-TagRFP-2A-tet3G

    Plasmid

    #167935

    Purpose

    Inducible CRISPRi expression

    Depositor

    Emma Rawlins

    Article

    Sun et al eLife. 2021 Oct 6;10. pii: 67886. doi: 1

    Insert

    KRAB-dCas9-DHFR
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    tetON

    Available since

    June 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLVX-EF1alpha-2xGFP:NES-IRES-2xRFP:NLS

    Plasmid

    #71396

    Purpose

    2Gi2R assay

    Depositor

    Fred Gage

    Article

    Mertens et al Cell Stem Cell. 2015 Oct 6. pii: S1934-5

    Inserts

    2xGFP:NES
    2xRFP:NLS
    

    Use

    Lentiviral

    Tags

    IRES

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha and IRES

    Available since

    Dec. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. mAzurite-16xRhoBAST

    Plasmid

    #196347

    Purpose

    Expression of mAzurite mRNA with 16xRhoBAST in 3'UTR

    Depositor

    Murat Sunbul

    Article

    Englert et al Nat Commun. 2023 Jun 30;14(1):3879. doi:

    Insert

    mAzurite-16xRhoBAST
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAMBgfp

    Plasmid

    #206130

    Purpose

    This binary vector has been generated for introduction of SGFP in filamentous fungi using ATMT. Contains the hygromycin resistance gene and the SGFP gene.

    Depositor

    Anne Osbourn

    Article

    Sesma et al Nature. 2004 Sep 30;431(7008):582-6. doi

    Insert

    hygromycin resistance gene and the Promoter of ToxA+SGFP
    

    Use

    Binary vector for expression of sgfp in filamento…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. mPlum-LaminB1-10

    Plasmid

    #55981

    Purpose

    Localization: Nuclear Envelope, Excitation: 590, Emission: 649

    Depositor

    Michael Davidson

    Insert

    LaminB1 (LMNB1 Human)
    

    Use

    Tags

    mPlum

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

16. TFORF0537

    Plasmid

    #141639

    Purpose

    Lentiviral vector for overexpressing the PAX2 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    PAX2 (PAX2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCI-neo-(HA)3-ubiquitin

    Plasmid

    #196991

    Purpose

    Expresses HA-tagged ubiquitin in mammalian cells

    Depositor

    Juan Bonifacino

    Article

    Mattera et al J Biol Chem. 2006 Mar 10;281(10):6874-83

    Insert

    ubiquitin (UBC Human)
    

    Use

    Tags

    triple HA tag

    Expression

    Mammalian

    Mutation

    silent mutation in ubiquitin codon 13

    Promoter

    CMV

    Available since

    March 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. TFORF0692

    Plasmid

    #143064

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    HNF4A (HNF4A Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. TFORF0689

    Plasmid

    #141662

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    HNF4G (HNF4G Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSBbi-Pur

    Plasmid

    #60523

    Purpose

    SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: puromycin resistance gene

    Depositor

    Eric Kowarz

    Article

    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

    Type

    Empty backbone

    Use

    Transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a/RPBSA

    Available since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. p7141 pHAGE-P-CMVt-N-HA-GAW-MUS81

    Plasmid

    #100157

    Purpose

    Expresses MUS81

    Depositor

    Peter Howley

    Article

    Galligan et al J Proteome Res. 2015 Feb 6;14(2):953-66.

    Insert

    MUS81 (MUS81 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pT3TS-spCas9-ABE-Umax-rest1

    Plasmid

    #222144

    Purpose

    Adenine base editor with modified TadA variant for restricted A-to-G base editing in zebrafish

    Depositor

    Gaurav Varshney

    Article

    Qin et al Nat Commun. 2024 Jul 4;15(1):5613. doi:

    Insert

    Modified TadA-nSpCas9
    

    Use

    CRISPR; Zebrafish expression

    Tags

    Bipartite NLS

    Expression

    Mutation

    Promoter

    T3

    Available since

    July 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. mKeima-Red-N1

    Plasmid

    #54597

    Purpose

    Localization: N1 Cloning Vector, Excitation: 440, Emission: 620

    Depositor

    Michael Davidson

    Type

    Empty backbone

    Use

    Tags

    mKeima-Red

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pBS31-cCARLIN

    Plasmid

    #160928

    Purpose

    Contains 10 constitutively expressed guide RNAs and CAG-GFP-CARLIN array sequence for DNA barcoding (when used in combination with Cas9)

    Depositor

    Fernando Camargo

    Article

    Bowling et al Cell. 2020 Jun 11;181(6):1410-1422.e27.

    Insert

    CARLIN gRNAs and CAG-GFP-CARLIN array
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter for gRNAs; CAG promoter for GFP

    Available since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pDRF1-GW yAT1.03R122KR126K

    Plasmid

    #132782

    Purpose

    Non-binding version of the FRET biosensor for ATP measurements in budding yeast

    Depositor

    Bas Teusink

    Article

    Botman et al ACS Sens. 2020 Mar 27;5(3):814-822. doi:

    Insert

    yAT1.03R122KR126K ymTq2-tdTomato
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    R122K, R126K

    Promoter

    Available since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. Lyn-R-GECO1

    Plasmid

    #120410

    Purpose

    Expresses a fusion between myristoylation signal peptide and R-GECO1. This construct targets R-GECO1 to the plasma membrane.

    Depositor

    Won Do Heo

    Article

    Kim et al Proc Natl Acad Sci U S A. 2016 May 24;11

    Insert

    Lyn-R-GECO1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. plYJP022-int7

    Plasmid

    #202287

    Purpose

    Plasmid containing integrase for LPattB7 site

    Depositor

    Christopher Voigt

    Article

    Triassi et al Cell Syst. 2023 Jun 21;14(6):512-524.e12

    Insert

    int7
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCDNA3.1(+)-NEMOc

    Plasmid

    #189933

    Purpose

    Mammalian expression of calcium sensor with ultra-high dynamics and sensitivity

    Depositor

    Yubin Zhou

    Article

    Li et al Nat Methods. 2023 Jun;20(6):918-924. doi

    Insert

    NEMOc
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. TFORF1898

    Plasmid

    #144116

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    PATZ1 (PATZ1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Flag-TRIM28

    Plasmid

    #124960

    Purpose

    Mammalian expression of Human TRIM28

    Depositor

    Michelle Barton

    Article

    Li et al Oncogene. 2017 May 25;36(21):2991-3001.

    Insert

    hTRIM28 (TRIM28 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pSCALPS-FLAG-HA-mmuMettl3

    Plasmid

    #207122

    Purpose

    Lentiviral plasmid to express mouse Mettl3

    Depositor

    Silvia Monticelli

    Article

    Leoni et al Nat Commun. 2023 Jun 29;14(1):3862. doi:

    Insert

    Mettl3 (Mettl3 Mouse)
    

    Use

    Lentiviral

    Tags

    FLAG-HA

    Expression

    Mutation

    Promoter

    SSFV

    Available since

    Dec. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. TFORF1141

    Plasmid

    #142736

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    HMBOX1 (HMBOX1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pUC19-TgKI-MCS-eGFP_Y40N-MCS-polyA

    Plasmid

    #174025

    Purpose

    This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

    Depositor

    Michel Bagnat

    Article

    Levic et al Development. 2021 Sep 8. pii: 272090. do

    Insert

    eGFP
    

    Use

    Zebrafish knock-in tagging

    Tags

    Expression

    Mutation

    Y40N

    Promoter

    Available since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pGP-CMV-NES-fRCaMP1

    Plasmid

    #125249

    Purpose

    Expresses red fluorescent calcium sensor in mammalian cells

    Depositor

    Katalin Torok

    Article

    Kerruth et al J Biol Chem. 2019 Mar 15;294(11):3934-39

    Insert

    fRCaMp1
    

    Use

    Tags

    NES signal

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pFH2.22_mCherry-MS2x8

    Plasmid

    #205537

    Purpose

    Express mCherry-MS2x8 (export tag consisting of 8 tandem repeats of MS2 stem-loop aptamer)

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV

    Available since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. mito-PAGFP

    Plasmid

    #23348

    Purpose

    Expresses a mitochondrial matrix-targeted photoactivable GFP that enables detection and quantification of organelle fusion in living cells.

    Depositor

    Richard Youle

    Article

    Karbowski et al J Cell Biol. 2004 Feb 16. 164(4):493-9.

    Insert

    PAGFP
    

    Use

    Tags

    mito

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 9, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pAAV.GFAP.Cre.WPRE.hGH

    Plasmid

    #105550

    Purpose

    AAV expression of Cre from GFAP promoter

    Depositor

    James M. Wilson

    Has Service

    AAV PHP.eB and AAV5

    Insert

    Cre
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    GFAP

    Available since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. Myc-Axin2

    Plasmid

    #21279

    Depositor

    Frank Costantini

    Article

    Jho et al Mol Cell Biol. 2002 Feb . 22(4):1172-83.

    Insert

    Axin2 (Axin2 Mouse)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 9, 2009

    Availability

    Academic Institutions and Nonprofits only

39. ATG9A-mCherry

    Plasmid

    #197393

    Purpose

    Expresses ATG9A in mammalian cells

    Depositor

    Juan Bonifacino

    Article

    De Pace et al PLoS Genet. 2018 Apr 26;14(4):e1007363.

    Insert

    ATG9A (ATG9A Human)
    

    Use

    Tags

    mCh

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. WT-eGFP

    Plasmid

    #215168

    Purpose

    Encodes the enhanced green fluorescent protein

    Depositor

    Travis Beddoe, Bhuvana Shanbhag

    Insert

    WT-eGFP
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 lac

    Available since

    June 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge