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pMaCTag-P13
Plasmid#120024PurposeTemplate for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mCherryDepositorInsertmCherry
UsePcr templateTagsmCherryExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
GFP-PS9
Plasmid#177944PurposeTransient mammalian expression of eGFP along with SARS-CoV-2 sequence 20080-21171 (PS9) within the 3'UTR. Resulting transcript is packaged into SARS-CoV-2 virus-like particles.DepositorInserteGFP (ORF1ab )
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Myc CDC4 WT*
Plasmid#16652DepositorInsertCDC4 (FBXW7 Human)
UseTagsmycExpressionMammalianMutation*Note that this insert is only WT relative to the…PromoterAvailable sinceMarch 12, 2008AvailabilityAcademic Institutions and Nonprofits only -
pSEVA451-Cpf1
Plasmid#192362PurposeCRISPR-based vector for genome editingDepositorInsertCas12a, gRNA scaffold
UseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAV-H1promoter-2xTetO-Tornado-2xALFA-10xSunTag
Plasmid#231591PurposeExpress circular RNA translation reporter with 2xALFATag-10xSunTag under H1 promoterDepositorInsertTornado-2xALFATag-10xSunTag
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-SUMO-MarathonRT
Plasmid#109029PurposeGroup II intron maturase from Eubacterium rectale (wild-type sequence). This is a highly processive reverse transcriptase that is called “MarathonRT”DepositorInsertERE_01490
UseTags6xHis-SUMO tagExpressionBacterialMutationPromoterT7Available sinceApril 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
Flag-FOXG1
Plasmid#153124PurposeExpression of Flag tagged human FOXG1 in mammalian cellsDepositorInserthuman FOXG1 (FOXG1 Human)
UseTagsFlagExpressionMammalianMutationPromoterCMV IE94Available sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-15b-UbcH5b/2419
Plasmid#11475DepositorInsertUbcH5b (UBE2D2 Human)
UseTagsExpressionBacterialMutationn/aPromoterAvailable sinceJuly 19, 2006AvailabilityAcademic Institutions and Nonprofits only -
GFP-NPM WT
Plasmid#17578DepositorInsertNPM (NPM1 Human)
UseTagsEGFP and FlagExpressionMammalianMutationPromoterAvailable sinceMarch 27, 2008AvailabilityAcademic Institutions and Nonprofits only -
HA-Clover
Plasmid#163366PurposeBackbone for generating N-terminal or C-terminal clover constructsDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2-QUAS:GFP-CAAX
Plasmid#184813PurposeUsed to generate the Tg(QUAS:GFP-CAAX)c591 transgenic lineDepositorInsertQUAS:GFP-CAAX-pA
UseZebrafish tol2 transgenesisTagsGFP-CAAXExpressionMutationPromoterAvailable sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
Flag-HA-USP10
Plasmid#22543DepositorInsertUSP10 (USP10 Human)
UseRetroviralTagsFLAG and HAExpressionMammalianMutationNonePromoterAvailable sinceJan. 20, 2010AvailabilityAcademic Institutions and Nonprofits only -
pLV_hU6-sgRNA_hUbC-dCas9-ZIM3-KRAB-T2a-PuroR
Plasmid#172982Purposecontrol & cloning vector for CRISPRi expressing dead Cas9-ZIM3-KRAB fusionDepositorInsertcontrol sgRNA
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBB550
Plasmid#27396DepositorInsertdnaK, dnaJ, GroESL
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 7, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTRE-Medium-FT
Plasmid#31914DepositorInsertMedium-FT
UseTagsExpressionMammalianMutationM18L/N23D/T43S/K69R/L84W/M152I/Q194L/L205M/Y221C/…PromoterPTight Tet-responsive PminCMVdAvailable sinceSept. 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET His6 MBP Asn10 TEV LIC cloning vector (1C)
Plasmid#29654DepositorTypeEmpty backboneUseTags10xAsn, 6xHis, MBP, and TEV cleavage siteExpressionBacterialMutationPromoterT7-lacO (lactose/IPTG inducible)Available sinceAug. 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET28:GFP
Plasmid#60733PurposepET28 driving expression of GFP, biobrick part Bba_E0040DepositorInsertGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceNov. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJRH-1187 VSVGmut
Plasmid#201913PurposeExpresses a VSV-G variant (K47Q R354A) defective for LDL-R receptor binding (CAG promoter)DepositorInsertVSV-G (K47Q, R354A)
UseTagsExpressionMammalianMutationK47Q, R354APromoterCAGAvailable sinceAug. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
mAIRN HO
Plasmid#207411PurposeMammalian-cell-based plasmid system that expresses mAIRN (an R-loop-forming transcript) upon dox-induction, which will further cause head-ON transcription-replication conflict (HO TRC).DepositorInsertmAIRN
UseTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
mAIRN CD
Plasmid#207412PurposeMammalian-cell-based plasmid system that expresses mAIRN (an R-loop-forming transcript) upon dox-induction, which will further cause co-directional transcription-replication conflict (CD TRC).DepositorInsertmAIRN
UseTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_cAMPFIRE-H
Plasmid#182281PurposeMammalian expression of the cAMP sensor cAMPFIRE-H under a CMV promotor.DepositorInsertcAMPFIRE-H (cAMP sensor)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pExp-MBP
Plasmid#112568PurposeUsed for expression of protein in E. coli as TEV cleavable N-terminual 8His and MBP fusions with optional C-terminal StrepII-tagDepositorTypeEmpty backboneUseTags8xHis-MBP and Strep tagExpressionBacterialMutationPromoterAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAL119-TK
Plasmid#21911DepositorInsertHSV Type 1 thymidine kinase
UseAdenoviralTagsExpressionMutationPromoterAvailable sinceOct. 21, 2009AvailabilityAcademic Institutions and Nonprofits only -
pHR-UCOE-SFFV-Zim3-dCas9-P2A-EGFP
Plasmid#188899PurposedCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS, and GFP linked by a P2A site from a SFFV promoter with an upstream ubiquitous chromatin opening elementDepositorInsertZim3-dCas9
UseLentiviralTagsHA-2xNLS, P2A-GFP, and Zim3 KRAB-NLS fusionExpressionMutationPromoterSFFVAvailable sinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
mKate2-P2A-APEX2-GBP
Plasmid#67650PurposeMammalian expression vector driving APEX2-GBP (EM peroxidase marker fused to GFP-targeting nanobody). Also contains bicistronially expressed cytoplasmic red fluorescent reporter.DepositorInsertAPEX2-GBP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTECH-MmAcK3RS(IPYE)
Plasmid#104071PurposeExpression plasmid encoding MmAcK3RS(IPYE) and pylTDepositorInsertPlpp MmAcK3RS(IPYE); PproK pyl
UseTagsExpressionBacterialMutationContains activating mutations V31I, T56P, H62Y, A…PromoterPlpp and PproKAvailable sinceDec. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGreenIIM DR5v2-ntdTomato/DR5-n3GFP
Plasmid#61628PurposeExpresses nuclear tdTomato from auxin-dependent DR5v2 (TGTCGG) promoter and nuclear 3xGFP from the DR5 (TGTCTC) promoterDepositorInsertsntdTomato
n2xEGFP
UseTagsExpressionPlantMutation2 copies of EGFP with N-terminal SV40 NLSPromoterAuxin-dependent DR5 promoter containing 9 reverse…Available sinceMarch 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCRIS-PITChv2-BSD-dTAG (BRD4)
Plasmid#91792PurposePITCh dTAG donor vector for BSD-P2A-2xHA-FKBP_F36V knock-in into the N-terminus of the human BRD4 locus.DepositorInsertBSD-P2A-2xHA-FKBP_F36V
UseCRISPRTagsExpressionMammalianMutationPhenylalanine 36 to valine in FKBP12PromoterPromoterlessAvailable sinceMarch 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHRIG-Akt1
Plasmid#53583Purposelentiviral expression of constitutively active human Akt1 and EGFPDepositorInsertcAkt1 (AKT1 Human)
UseLentiviralTagsHA and IRES-EGFPExpressionMammalianMutationconstitutively active (myristoylated version)PromoterCMVAvailable sinceOct. 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBEVY-GL
Plasmid#51225Purposebi-directional expression vector for yeast, galactose inducible, LEU2 selectionDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterGAL1,10Available sinceMarch 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-CDK4
Plasmid#116724PurposeLentiviral expression of CDK4DepositorInsertCDK4 (CDK4 Human)
UseLentiviralTagsExpressionMutationWTPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
TUBB5-Halo
Plasmid#64691PurposeExpresses beta-tubulin labeled with Halo-tagDepositorInsertTUBB
UseTagsHalo-tagExpressionMammalianMutationPromoterAvailable sinceJune 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
CRY2high-mCherry
Plasmid#104063PurposeExpresses fusion of CRY2high mutant (CRY2PHR E490R) with mCherryDepositorInsertCRY2high-mCherry
UseTagsExpressionMammalianMutationCRY2PHR E490RPromoterCMVAvailable sinceDec. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-lambdaN-HA-HsEDC4
Plasmid#66597PurposeExpresses lambdaN-HA-tagged EDC4 in mammalian cellsDepositorInsertEDC4 (EDC4 Human)
UseTagslambdaN-HAExpressionMammalianMutationPromoterCMVAvailable sinceJune 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA2-ROC1
Plasmid#19897DepositorInsertRING finger protein 1 (ROC1) (RBX1 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceDec. 31, 2008AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEx-ER-TurboID
Plasmid#160857PurposeCre recombinase-dependent expression of ER TurboID, N-term HA tag, C-term V5 tag, in AAV expression plasmid. Amp selectionDepositorInsertER TurboID
UseAAVTagsHA and V5ExpressionMutationPromoterCAGAvailable sinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG-FLEX.SF-iGluSnFR.A184S
Plasmid#106186PurposeTight affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **DepositorInsertSF-iGluSnFR.A184S
UseAAVTagsExpressionMutationGltI: A184SPromoterCAGAvailable sinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
SaCas9_sgRNA_expression_in_pBluescript
Plasmid#122090PurposeU6 driven SaCas9 sgRNA expression vector for cloning own guidesDepositorInsertSaCas9 tracr (NEWENTRY S. pyogenes, Synthetic)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28a-sfGFP
Plasmid#85492PurposepET28a expressing codon optimized superfolder GFP with C-terminal His6 tagDepositorInsertsuperfolder GFP
UseTags6x HisExpressionBacterialMutationPromoterT7Available sinceJan. 10, 2017AvailabilityAcademic Institutions and Nonprofits only
