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pT7-7 asyn WT

Plasmid

#36046

Depositor

Hilal Lashuel

Article

Paleologou et al J Biol Chem. 2008 Jun 13;283(24):16895-9

Insert

alpha-synuclein (SNCA Human)

Expression

Bacterial

Promoter

T7

Available Since

July 13, 2012

Availability

Academic Institutions and Nonprofits only

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pHiUGE TurboID-HA donor (all-in-one) ORF2

Plasmid

#200385

Purpose

Tagging endogenously expressed proteins with TurboID-HA, C-terminal

Depositor

Scott Soderling

Article

Gao et al Nat Commun. 2024 Aug 9;15(1):6801. doi:

Insert

TurboID (ref: PMID: 30125270, Addgene plasmid # 107169)

Expression

Mammalian

Mutation

NA

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pB-T-PAF-Clu

Plasmid

#215380

Purpose

High expression of Clusterin for purification.

Depositor

Franz-Ulrich Hartl

Insert

CLU (CLU Human)

Expression

Mammalian

Promoter

tetO

Available Since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-TadA8e-BLM

Plasmid

#221226

Purpose

Express HACE Editor as a fusion of TadA-8e adenosine deaminase and BLM helicase.

Depositor

Fei Chen

Article

Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

Insert

TadA8e-BLM

Expression

Mammalian

Available Since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pmGFP-P2A-K0-P2A-RFP

Plasmid

#105686

Purpose

Double fluorescent stalling reporter K0

Depositor

Ramanujan Hegde

Article

Juszkiewicz et al Mol Cell. 2017 Feb 16;65(4):743-750.e4.

Inserts

P2A-3xFLAG-VHPbeta
K0
P2A-mCherry

Expression

Mammalian

Promoter

CMV (from vector)

Available Since

Feb. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pT2-shP53

Plasmid

#124261

Purpose

Expresses shRNA targeting P53. Construct has inverted repeats to be used in Sleeping beauty system.

Depositor

Maria Castro

Article

Nunez et al Sci Transl Med. 2019 Feb 13;11(479). pii

Insert

shP53

Expression

Mammalian

Available Since

April 8, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3-endo-EKAR4

Plasmid

#205513

Purpose

Genetically encoded FRET-based biosensor for monitoring ERK kinase activity. Targeted to endosomes.

Depositor

Jin Zhang

Article

Kwon et al Nature. 2022 Nov;611(7934):173-179. doi:

Insert

endo-EKAR4

Tags

2xFYVE (Fab1/YOTB/Vac1/EEA1 zinc-finger) and 6xHI…

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-7 asyn A53T

Plasmid

#105727

Purpose

Bacterial expression of mutant human alpha synuclein

Depositor

Hilal Lashuel

Article

Rospigliosi et al J Mol Biol. 2009 May 22;388(5):1022-32.

Insert

alpha-synuclein A53T (SNCA Human)

Expression

Bacterial

Mutation

A53T

Promoter

T7

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pRK5-FLAG-S6K1

Plasmid

#100509

Purpose

Expresses FLAG tagged S6K1 in mammalian cells

Depositor

David Sabatini

Article

Gu et al Science. 2017 Nov 10;358(6364):813-818.

Insert

S6K1 (Rps6kb1 Rat)

Tags

FLAG

Expression

Mammalian

Available Since

Nov. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pAAVone-AAV9-CMV-eGFP

Plasmid

#230928

Purpose

pAAVone plasmid is used to package AAV-CMV-eGFP with an AAV9 serotype, utilizing the AAVone single-plasmid AAV production system.

Depositor

Qizhao Wang

Article

Yang et al Mol Ther Nucleic Acids. 2025 May 14;36(2

Insert

eGFP

Use

AAV

Promoter

CMV

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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TRE-UBC-H2-Kb SCT-T2A-GFP

Plasmid

#205454

Purpose

lentiviral plasmid for expression of mouse H2-Kb SCT

Depositor

Deepta Bhattacharya

Article

Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

Insert

H2-Kb SCT (H2-K1 Mouse)

Expression

Mammalian

Promoter

UBC

Available Since

Oct. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pINDUCER13 (miR-LUP)

Plasmid

#46936

Purpose

Inducible lentiviral gene silencing vector. Insert PheSGly294, (XhoI to EcoRI; 1.4kb), can be replaced w mir30 based hairpin of interest

Depositor

Thomas Westbrook

Article

Meerbrey et al Proc Natl Acad Sci U S A. 2011 Mar 1;108

Insert

PheS Gly294

Use

Lentiviral

Available Since

Nov. 4, 2014

Availability

Academic Institutions and Nonprofits only

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psiCheck2-SV40p-3xFLAG-HSVTKp-mCherry

Plasmid

#182377

Purpose

Dual expression construct encoding 3xFLAG and mCherry from separate promoters

Depositor

Chunghun Lim

Article

Lee et al BMC Biol. 2022 Jan 7;20(1):12. doi: 10.1

Inserts

3xFlag
mCherry

Expression

Mammalian

Promoter

HSV TK promoter and SV40 promoter

Available Since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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pIVT-mGL

Plasmid

#232768

Purpose

IVT template for making modRNA encoding mGreenLantern

Depositor

Kate Galloway

Article

Blanch-Asensio et al Nat Biomed Eng. 2026 Apr 30. doi: 10.103

Insert

mGreenLantern

Use

In vitro transcription

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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GFP_CD47_SU

Plasmid

#65473

Purpose

GFP tagged CD47 with its own short 3'UTR

Depositor

Christine Mayr

Article

Berkovits et al Nature. 2015 Apr 20. doi: 10.1038/nature

Inserts

Signal peptide of CD47
GFP
CD47 coding region continued and short 3’UTR.

Expression

Mammalian

Available Since

June 25, 2015

Availability

Academic Institutions and Nonprofits only

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SOX9-T2A-H2B-EGFP repair template

Plasmid

#167972

Purpose

SOX9 reporter repair template

Depositor

Emma Rawlins

Article

Sun et al eLife. 2021 Oct 6;10. pii: 67886. doi: 1

Insert

SRY-Box Transcription Factor 9 (SOX9 Human)

Use

CRISPR; Donor template

Tags

H2B-EGFP

Expression

Mammalian

Mutation

homology arms contain point mutations to disrupt …

Available Since

June 7, 2021

Availability

Academic Institutions and Nonprofits only

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EFNB1-bio-His

Plasmid

#51749

Purpose

Expresses full-length Ephrin-B1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

Depositor

Gavin Wright

Article

Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

Insert

EFNB1 (EFNB1 Human)

Tags

His tag, enzymatic biotinylation sequence, and ra…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-BaLgp120-8his

Plasmid

#100925

Purpose

Mammalian expression plasmid for gp120 subunit from the BaL HIV-1 strain

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (BaL) Env

Tags

CD5 leader sequence and Purification tag after K5…

Expression

Mammalian

Mutation

Codon-optimized synthetic gene

Promoter

CMV

Available Since

March 25, 2019

Availability

Academic Institutions and Nonprofits only

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pTREtight2

Plasmid

#19407

Depositor

Markus Ralser

Type

Empty backbone

Expression

Mammalian

Available Since

Oct. 15, 2008

Availability

Academic Institutions and Nonprofits only

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mRNA-CleanCap entry vector

Plasmid

#188249

Purpose

Cloning vector for generating template plasmids to transcribe mRNA, with mNeonGreen dropout following BsaI digestion

Depositor

Howard Chang

Article

Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

Insert

mNeonGreen

Expression

Mammalian

Promoter

T7

Available Since

Sept. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pLX304-DCK*-GW-IRES-GFP

Plasmid

#176289

Purpose

Gateway vector for use in generating DCK*-POI fusion reporter

Depositor

William Kaelin

Article

Koduri et al Sci Adv. 2021 Feb 5;7(6). pii: 7/6/eabd6

Insert

Mutant Deoxycytidine Kinase (DCK*, S74E R104M D133A) (DCK Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

S74E R104M D133A

Promoter

CMV

Available Since

March 23, 2023

Availability

Academic Institutions and Nonprofits only

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pBad-PhoCl2c

Plasmid

#164035

Purpose

Improved PhoCl variant with enhanced dissociation efficiency

Depositor

Robert Campbell

Article

Lu et al Chem Sci. 2021 May 31;12(28):9658-9672.

Insert

PhoCl2c

Tags

His tag, T7 tag, Xpress Tag

Expression

Bacterial

Promoter

araBad promotor

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pCX419

Plasmid

#229216

Purpose

All-in-one plasmid for labeling of XIST RNA via Csm complex

Depositor

Jennifer Doudna

Article

Xia et al Nat Biotechnol. 2025 Feb 18. doi: 10.103

Insert

Csm1; Csm2, Csm3(RNase mut)-sfGFP; Csm4; Csm5; Cas6; XIST crRNA

Expression

Mammalian

Available Since

Feb. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pG1C

Plasmid

#71740

Purpose

Modular shuttle vector for Geobacillus and E. coli

Depositor

Tom Ellis

Article

Reeve et al ACS Synth Biol. 2016 Jul 1.

Type

Empty backbone

Use

Synthetic Biology; Shuttle vector

Expression

Bacterial

Available Since

July 14, 2016

Availability

Academic Institutions and Nonprofits only

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Ad-Flag-XBP1s

Plasmid

#63678

Purpose

Adenoviral vector for XBP1s expression

Depositor

Qingbo Xu, Lingfang Zeng

Article

Zeng et al Proc Natl Acad Sci U S A. 2009 May 19;10

Insert

XBP1 (XBP1 Human)

Use

Adenoviral

Tags

Flag

Expression

Mammalian

Mutation

spliced

Available Since

March 23, 2015

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(Empty)-PGKmCherry2ABFP-W

Plasmid

#67985

Purpose

Cas9 activity reporter (control) with mCherry and BFP

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

U6gRNA cassette, PGKmCherryABFP cassette, WPRE

Use

CRISPR and Lentiviral

Mutation

Deleted BbsI site within WPRE, modified BFP

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pU6-hROSA26-1-1kbdonor

Plasmid

#234736

Purpose

PseCAST crRNA targeting hROSA26-1, with 1 kb donor transposon

Depositor

David Liu

Article

Witte et al Science. 2025 May 15;388(6748):eadt5199.

Insert

hROSA26-1 crRNA

Expression

Mammalian

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pETSXM2-pLacI-mtrCAB

Plasmid

#174618

Purpose

Bacterial expression of gene cluster mtrCAB from Shewanella oneidensis MR-1.

Depositor

I-Son Ng

Article

I-Son Ng et al Bioresour. Bioprocess. 5, 35 (2018)

Insert

mtrCAB

Use

Synthetic Biology

Expression

Bacterial

Promoter

pLacI

Available Since

Nov. 2, 2021

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2 puro - CRBN (#1)

Plasmid

#166240

Purpose

sgRNA (#1) to generate a knockout of human CRBN by targeting the middle region of Exon1

Depositor

Günter Schneider

Article

Lier et al Bioorg Chem. 2021 Nov 20:105505. doi: 10

Insert

CRBN (CRBN Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Dec. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti6MQBI163 SGLT2

Plasmid

#194147

Purpose

Expresses SGLT2 protein in mammalian cells

Depositor

Jun Liu

Insert

SLC5A2 (SLC5A2 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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