We narrowed to 21,485 results for: SPR

Showing: 5081 - 5120 of 21485 results

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pBS-GMR-eya(shRNA)

Plasmid

#157991

Purpose

pBSII-KS(-)-based vector backbone containing a dominant eye marker for constructing Drosophila CRISPR HDR templates. Marker allows counter selection against vector integration events.

Depositor

Greg Beitel

Article

Nyberg et al Fly (Austin). 2020 Oct 5. doi: 10.1080/1

Type

Empty backbone

Use

CRISPR

Available Since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pJA50

Plasmid

#59931

Purpose

gRNA for cleavage at unc-58(e665) locus in C elegans

Depositor

Andrew Fire

Article

Arribere et al Genetics. 2014 Aug 26. pii: genetics.114

Insert

unc-58(e665) gRNA

Use

CRISPR

Promoter

U6

Available Since

Sept. 22, 2014

Availability

Academic Institutions and Nonprofits only

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sgTrack-Gateway

Plasmid

#114011

Purpose

3rd generation lentiviral empty sgRNA expression vector with a Gateway cassette for co-expression of a reporter cDNA

Depositor

Charles Rudin

Article

Hulton et al Nat Cancer. 2020 Mar;1(3):359-369. doi:

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

March 10, 2020

Availability

Academic Institutions and Nonprofits only

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pSL1433

Plasmid

#129523

Purpose

pDEF-SpCas9::GFP, Empty Vector for RGR and HDR Template, PyBiP-minimal promoter, 3rd Generation

Depositor

Scott Lindner

Article

Walker et al J Biol Chem. 2019 Jun 14;294(24):9555-95

Insert

SpCas9 (Other)

Use

Plasmodium, rodent-infectious species

Available Since

Sept. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pAL-pck_NT

Plasmid

#74069

Purpose

pAL374 plasmid carrying the pck (NT) sgRNA, targeting the non-template strand of pck, SpecR

Depositor

Timothy Lu

Article

Cleto et al ACS Synth Biol. 2016 Feb 16.

Insert

sgRNA pck (NT)

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

ptac

Available Since

April 12, 2016

Availability

Academic Institutions and Nonprofits only
qTAG-AAVS1-Ef1a-Blast

Plasmid

#227267

Purpose

Empty AAVS1 targeting donor for the insertion of Blast and a strong EF1a promoter. A CDS can be cloned adjacent to the promoter via restriction or gibson cloning using the MluI cut site.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pRDB_054

Plasmid

#216096

Purpose

All-in-one, knockout, delivers Cas12a & gRNA

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

Cas12a [EnAs]

Use

CRISPR and Lentiviral; Assembled vector

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pRU48

Plasmid

#167670

Purpose

ENTRY vector for gRNA8 cloning under pAtU3 promoter

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

pAtU3

Use

CRISPR

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pCAT007

Plasmid

#171634

Purpose

Plasmid containing a U6 promoter expressing a spyCas9 sgRNA targeting TRAC and CAG promoter expressing mTagBFP2

Depositor

Jennifer Doudna

Article

Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

Inserts

spyCas9 sgRNA-TRAC
mTagBFP2

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

CAG and U6

Available Since

June 30, 2021

Availability

Academic Institutions and Nonprofits only

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pKIKan_idCas9_Chr_center

Plasmid

#149637

Purpose

suicide vector for knock in into the B. burgdorferi chromosome

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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Anep8_Cas9

Plasmid

#117169

Purpose

Expressed Cas9 in Aspergillus with LIC tags for easy gRNA cloning

Depositor

Adrian Tsang

Article

Song et al PLoS One. 2018 Aug 24;13(8):e0202868. do

Type

Empty backbone

Use

CRISPR and Synthetic Biology; Filamentous fungi e…

Promoter

Pki

Available Since

Jan. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pAi14-luc-MC

Plasmid

#87113

Purpose

minicircle parental backbone plasmid including luc-pA knock-in donor and one cutting site for HITI

Depositor

Juan Belmonte

Article

Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

Insert

Luciferase-SV40pA

Use

CRISPR and Luciferase

Available Since

March 31, 2017

Availability

Academic Institutions and Nonprofits only

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pBK2044-AAV-2xSp1-2xNFkB-EFSNC-dSaCas9-KRAB-MECP2

Plasmid

#223164

Purpose

2nd gen. vector for expression of KRAB and truncated MECP2 with dSaCas9 and empty gRNA scaffold

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Insert

KRAB-MECP2 (MECP2 Human)

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Encodes aa 204-310 (MECP2)

Promoter

EF1a

Available Since

Aug. 21, 2024

Availability

Academic Institutions and Nonprofits only

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P3-dCas9-Tet1-GFP-Puro

Plasmid

#190728

Purpose

Expression of human spdCas9-Tet1 fusion for targeted DNA methylation in mammalian cell. EGFP-puromycin double marker under an independent CMV promoter. Cloning backbone for spgRNA (BbsI)

Depositor

Maja Jagodic

Article

Pahlevan Kakhki et al Nat Commun. 2024 Jul 30;15(1):6419. doi:

Insert

dCas9-tet1

Use

CRISPR

Tags

3XFLAG and SV40 NLS

Expression

Mammalian

Mutation

Changed Pro434 codon from CCG to CCC and Gly468 f…

Promoter

pCAG

Available Since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

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pJT300 Lenti pEF-dCas9-3XFLAG-ZNF705_KRAB-T2A-tagBFP-P2A-Blast

Plasmid

#195542

Purpose

dCas9 effector fusion for manipulating transcription

Depositor

Michael Bassik, Lacra Bintu

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

Lenti pEF-dCas9-3XFLAG-ZNF705_KRAB-T2A-tagBFP-P2A-Blast

Use

CRISPR and Lentiviral

Tags

3XFLAG

Expression

Mammalian

Promoter

pEF1a

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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AKAP1-hLOV-TEVcs-GAL4bd-VP64-V5

Plasmid

#170995

Purpose

HiLITR transcription factor with AKAP1 N-terminal tmd targeting sequence (mitochondria)

Depositor

Alice Ting

Article

Coukos et al Elife. 2021 Aug 20;10. pii: 69142. doi:

Insert

AKAP1(tmd)-NNES-hLOV-TEVcs(ENLYFQ/M)-GAL4bd-VP64-V5 (AKAP1 Synthetic)

Use

Lentiviral and Synthetic Biology

Tags

V5

Expression

Mammalian

Promoter

Ef1-alpha

Available Since

Aug. 19, 2021

Availability

Academic Institutions and Nonprofits only

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Lenti-UPEmS-tevo

Plasmid

#228894

Purpose

Golden gate cloning (BsmBI) of pegRNAs with tevopreQ1 motif, and with constitutive RFP expression.

Depositor

Francisco J Sánchez-Rivera

Article

Gould et al Nat Biotechnol. 2024 Mar 12. doi: 10.103

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pegRNA2_tevopreQ1

Plasmid

#212039

Purpose

Bacterial expression of the pegRNA for genome editing in E. coli

Depositor

Quanjiang Ji

Article

Zhang et al Nat Commun. 2024 Jan 27;15(1):825. doi:

Insert

pegRNA

Use

CRISPR

Promoter

J23119

Available Since

April 12, 2024

Availability

Academic Institutions and Nonprofits only

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pUF-Cas9-pre-sgRNA

Plasmid

#137845

Purpose

Cas9 and gRNA expression plasmid for P. falciparum with yDHODH selectable marker.

Depositor

Josh Beck

Article

Nessel et al Cell Microbiol. 2020 Jan 28:e13168. doi:

Type

Empty backbone

Use

CRISPR

Available Since

May 18, 2020

Availability

Academic Institutions and Nonprofits only

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pRec ΔLacI

Plasmid

#104575

Purpose

Expresses Cas1, Cas2 under PLTetO-1 promoter, and TetR constitutively.

Depositor

Harris Wang

Article

Sheth et al Science. 2017 Dec 15;358(6369):1457-1461

Insert

PTet-cas12, tetR

Use

Synthetic Biology

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pCasY1

Plasmid

#87687

Purpose

p15A plasmid expressing CasY1

Depositor

Jennifer Doudna

Article

Burstein et al Nature. 2016 Dec 22. doi: 10.1038/nature

Insert

CasY1

Expression

Bacterial

Available Since

April 27, 2017

Availability

Academic Institutions and Nonprofits only

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pACYC-Cas12g1-noncoding

Plasmid

#120880

Purpose

Expresses concatenated noncoding sequences surrounding the CRISPR-Cas12g1 system in the native genomic loci; expresses the tracrRNA required for activity.

Depositor

Arbor Biotechnologies

Article

Yan et al Science. 2019 Jan 4;363(6422):88-91. doi

Insert

Noncoding sequences for CRISPR-Cas12g1

Use

CRISPR

Expression

Bacterial

Promoter

lac

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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GluN2B (px330:2-guideCas9)

Plasmid

#169430

Purpose

Expression of Cas9 and 2 guides

Depositor

Richard Huganir

Article

Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

Insert

spCas9

Use

CRISPR

Expression

Mammalian

Available Since

June 21, 2021

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-mTagBFP2-2A-Puro_hU6-DJ-DR36-BsmBI

Plasmid

#226009

Purpose

DjCas13d guide RNA cloning backbone (lenti)

Depositor

Silvana Konermann

Article

Wei et al Cell Syst. 2023 Dec 20;14(12):1087-1102.

Type

Empty backbone

Use

Lentiviral

Available Since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pPfetFn-Target_v2

Plasmid

#199564

Purpose

gRNA expression for FnCas12a in E. coli and clostridia

Depositor

Philippe Lambin

Article

Zhang et al Microbiol Spectr. 2023 Nov 10:e0245923.

Insert

SacB

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

Pthl_fU

Available Since

Nov. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pMiniT:GFP-Gephyrin TKIT donor

Plasmid

#169433

Purpose

Donor DNA for TKIT knockin of GFP to Gephyrin in mouse

Depositor

Richard Huganir

Article

Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

Insert

EGFP

Use

CRISPR

Expression

Mammalian

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ290

Plasmid

#129670

Purpose

Gateway entry clone for CRISPR-Cas12b systems

Depositor

Yiping Qi

Article

Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

Insert

AacCas12b

Use

CRISPR; Gateway compatible aaccas12b entry clone

Expression

Plant

Mutation

AacCas12b is rice codon optimized

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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pYPQ292

Plasmid

#129672

Purpose

Gateway entry clone for CRISPR-Cas12b systems

Depositor

Yiping Qi

Article

Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

Insert

AaCas12b

Use

CRISPR; Gateway compatible aacas12b entry clone

Expression

Plant

Mutation

AaCas12b is rice codon optimized

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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pV1081

Plasmid

#111425

Purpose

Solo vector for expression of CaCas9 and sgRNA for CaADE2 mutagenesis - integrates at ENO1

Depositor

Gerald Fink

Article

Vyas et al Sci Adv. 2015;1(3):e1500248.

Insert

CaCas9/sgADE-NT2

Use

CRISPR

Expression

Yeast

Available Since

Oct. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pAN-NOR

Plasmid

#62306

Purpose

synthetic circuit: NOR gate

Depositor

Christopher Voigt

Article

Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

Inserts

A2NT sgRNA
A2NT sgRNA

Use

CRISPR

Expression

Bacterial

Promoter

PPhlF and pBAD

Available Since

April 8, 2015

Availability

Academic Institutions and Nonprofits only

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pYPQ142-ZmUbi

Plasmid

#138106

Purpose

Golden Gate recipient and Gateway entry vector; assembly of 2 gRNAs driven by Zea mays Ubi promoter

Depositor

Yiping Qi

Article

Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pYPQ144-ZmUbi-pT

Plasmid

#138108

Purpose

Golden Gate recipient and Gateway entry vector; assembly of 4 gRNAs driven by Zea mays Ubi promoter

Depositor

Yiping Qi

Article

Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pMOD_A1511

Plasmid

#91037

Purpose

Module A, Promoter: OsAct1, Gene: Csy4-P2A-TaCas9, Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Csy4-P2A-TaCas9

Use

CRISPR

Promoter

OsAct1

Available Since

July 10, 2017

Availability

Academic Institutions and Nonprofits only
pMOD_A0508

Plasmid

#91014

Purpose

Module A, Promoter: EC1.2, Gene: Csy4-P2A-AtCas9, Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Csy4-P2A-AtCas9

Use

CRISPR

Promoter

EC1.2

Available Since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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reporter-gT2

Plasmid

#47321

Purpose

TF reporter for gRNA-AAVS1_T2 (RNA-guided dTomato expression)

Depositor

George Church

Article

Mali et al Nat Biotechnol. 2013 Aug 1. doi: 10.1038

Insert

minCMV-dTomato_pA

Use

CRISPR

Available Since

Aug. 16, 2013

Availability

Academic Institutions and Nonprofits only
pLenti_Cas9_T2A_mNeonGreen_P2A_blasticidin

Plasmid

#211471

Purpose

lentiviral tricistronic complex expressing Cas9, mNeonGreen, and blasticidin resistance

Depositor

James Eshleman

Article

Teh et al NAR Cancer. 2024 Jun 19;6(2):zcae028. do

Insert

Cas9_T2A_mNeonGreen_P2A_BlasticidinR

Use

CRISPR and Lentiviral

Tags

3xFlag

Expression

Mammalian

Promoter

hU6, EF-1a

Available Since

March 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBA615

Plasmid

#186239

Purpose

eLbuCas13a Negative Control (RFP targeting)

Depositor

Jennifer Doudna

Article

Adler et al Nat Microbiol. 2022 Dec;7(12):1967-1979.

Insert

pTet-eLbuCas13a + crRNA negative control

Expression

Bacterial

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pCRISPomyces-SaCas9

Plasmid

#129553

Purpose

Contains codon-optimized SaCas9 and gRNA for streptomyces

Depositor

Fong Tian Wong, Mingzi M. Zhang

Article

Yeo et al Biotechnol Bioeng. 2019 May 15. doi: 10.

Insert

SaCas9

Expression

Bacterial

Promoter

rpsL(XC)-BbsI

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pHRdSV40_scFv_GCN4_sfGFP_p65-hsf1_GB1_NLS

Plasmid

#79372

Purpose

Recruits p65-hsf1 to a compatible Cas9 protein for transcriptional activation

Depositor

George Church

Article

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Insert

GCN4-sfGFP-p65-hsf1

Use

CRISPR

Expression

Mammalian

Available Since

March 6, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pJMP1358

Plasmid

#119277

Purpose

for cloning new sgRNAs

Depositor

Carol Gross, Jason Peters, Oren Rosenberg

Article

Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

Insert

dcas9

Expression

Bacterial

Mutation

D10A and H840A

Available Since

Jan. 14, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 5081 - 5120 of 21485 results

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We narrowed to 21,485 results for: SPR