Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

163,572 results

Showing: 6451 - 6500 of 163572 results

  2. AP1muA-mStayGold-polyA-Hygro HR

    Plasmid
    #229679
    Purpose
    Homology repair plasmid for endogenous tagging of AP1muA at the C-terminus with monomeric StayGold. Contains a hygromycin resistance cassette for selection of edited cells
    Depositor
    Francesca Bottanelli
    Article
    Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/
    Insert
    genomic sequence AP1M1 locus (AP1M1 Human)
    Use
    CRISPR
    Tags
    monomeric StayGold
    Expression
    Mutation
    Promoter
    Available Since
    Jan. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    229679-plasmid-map-sequence-id-457128

    Enlarge

  4. pET21a-Streptavidin-Dead

    Plasmid
    #20859
    Purpose
    Dead subunit for creating monovalent streptavidin with a single femtomolar biotin binding site. Use in combination with Streptavidin-Alive (construct 20860).
    Depositor
    Alice Ting
    Article
    Howarth et al Nat Methods. 2006 Apr . 3(4):267-73.
    Insert
    Streptavidin-Dead
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Triple mutant of wt Streptavidin N23A,S27D,S45A
    Promoter
    Available Since
    April 21, 2009
    Availability
    Academic Institutions and Nonprofits only
    20859-plasmid-map-sequence-id-220527

    Enlarge

  13. pLJM1 EGFP-Tau

    Plasmid
    #108868
    Purpose
    Expresses EGFP linked to human Tau
    Depositor
    Gabriele Kaminski Schierle
    Insert
    Tau (MAPT Human)
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    human four-repeat tau lacking the N-terminal sequ…
    Promoter
    CMV
    Available Since
    May 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    108868-plasmid-map-sequence-id-237064

    Enlarge

  23. pSAM_Cam

    Plasmid
    #91570
    Purpose
    Mariner transposon mutagenesis & sequencing vector - Transposon contains KanR and Illumina sequencing adapters. Lac promoter expresses transposase. Chloramphenicol resistance on backbone.
    Depositor
    Harry Mobley
    Article
    Bachman et al MBio. 2015 Jun 9;6(3):e00775. doi: 10.11
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Aug. 10, 2017
    Availability
    Academic Institutions and Nonprofits only

  26. pHH21-BNA-Luc

    Plasmid
    #187924
    Purpose
    Expresses Luciferase reporter RNA construct under the 5' and 3' UTRs of NA segment of Influenza B/Brisbane/60/2008 virus by RNA polymerase I in mammalian cells
    Depositor
    Arindam Mondal
    Article
    Kedia et al Front Microbiol. 2022 May 25;13:868367.
    Insert
    Reporter RNA (RNA encoding firefly luciferase under the viral promoter of segment 6 of Influenza B/Brisbane/60/2008 virus)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    RNA polymease I Promoter
    Available Since
    Sept. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    187924-plasmid-map-sequence-id-373471

    Enlarge

  29. TRIPZ-bioGEF-UBnc-PURO

    Plasmid
    #208044
    Purpose
    Enables inducible expression of low-affinity Avitag-UBnc; to perform bioE3; nc = non-cleavable mutation; selection with puromycin
    Depositor
    James Sutherland, Rosa Barrio
    Article
    Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:
    Insert
    UBnc (UBC Human)
    Use
    Lentiviral
    Tags
    Low-affinity Avitag
    Expression
    Mutation
    L73P Mutation near C-terminus suppresses cleavage…
    Promoter
    tetO/UBC
    Available Since
    Nov. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    208044-plasmid-map-sequence-id-410266

    Enlarge

  30. pAIDA1

    Plasmid
    #79180
    Purpose
    A construct with the AIDA surface expression system for display of a passenger protein flanked by His and Myc tags
    Depositor
    Gen Larsson
    Article
    Jarmander et al Microb Cell Fact. 2012 Sep 3;11:118. doi
    Insert
    Adhesin Involved in Diffuse Adherence
    Use
    Tags
    His and Myc
    Expression
    Bacterial
    Mutation
    Promoter
    lacUV5
    Available Since
    Jan. 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    79180-plasmid-map-sequence-id-170854

    Enlarge

  34. GLP1R-DuET

    Plasmid
    #213253
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    GLP1R (GLP1R Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213253-plasmid-map-sequence-id-430803

    Enlarge

  35. pOT_6 - lenti-EFS-FMC6.3-BBz-2A-puro-2A-tNGFR

    Plasmid
    #181975
    Purpose
    Expresses anti-CD19 CAR (with 4-1BB signaling domain) linked to puromycin resistance via P2A and to human truncated NGFR via T2A for lentiviral delivery.
    Depositor
    Neville Sanjana
    Article
    Legut et al Nature. 2022 Mar;603(7902):728-735. doi:
    Insert
    FMC6.3-BBz CAR; tNGFR (NGFR Human, Synthetic)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    NGFR truncation: aa 11-276
    Promoter
    Available Since
    April 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    181975-plasmid-map-sequence-id-377682

    Enlarge

  41. pSBbi-Bla

    Plasmid
    #60526
    Purpose
    SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: blasticidin resistance gene
    Depositor
    Eric Kowarz
    Article
    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b
    Type
    Empty backbone
    Use
    Transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a/RPBSA
    Available Since
    Feb. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    60526-plasmid-map-sequence-id-97520

    Enlarge

  44. GST-PR-LBD

    Plasmid
    #89117
    Purpose
    bacterial expression of human progesterone receptor ligand binding domain amino acid residues 637-933 with GST tag in pGEX-3X-1
    Depositor
    Elizabeth Wilson
    Insert
    human progesterone receptor ligand binding domain (PGR Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    contains Ligand Binding Domain (637-933)
    Promoter
    tac
    Available Since
    April 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    89117-plasmid-map-sequence-id-175728

    Enlarge

  45. pcDNA3.1-3'TwStrep

    Plasmid
    #208618
    Purpose
    Empty vector for mammalian expression, insert Twin Strep tag using BamHI and AflII site of pcDNA3.1 (-), both site retained, BamHI in-frame with Twin Strep tag
    Depositor
    Quanfu Ma
    Type
    Empty backbone
    Use
    Tags
    Twin Strep
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208618-plasmid-map-sequence-id-418314

    Enlarge

Showing: 6451 - 6500 of 163572 results