Skip to main content
Addgene
Showing: 6451 - 6500 of 157653 results

  5. pJL 89

    Plasmid
    #98558
    Purpose
    Useful for making infectious clones of RNA virus cDNAs; Has 35S promoter and HDV Ribozyme sequences in T-DNA region of Agrobacterium vector.
    Depositor
    John Lindbo
    Article
    Lindbo et al Plant Physiol. 2007 Dec;145(4):1232-40.
    Type
    Empty backbone
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Enh 35S
    Available since
    Aug. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    98558-plasmid-map-sequence-id-192654

    Enlarge

  11. GFP-PS9

    Plasmid
    #177944
    Purpose
    Transient mammalian expression of eGFP along with SARS-CoV-2 sequence 20080-21171 (PS9) within the 3'UTR. Resulting transcript is packaged into SARS-CoV-2 virus-like particles.
    Depositor
    Jennifer Doudna
    Article
    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11
    Insert
    eGFP (ORF1ab )
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    177944-plasmid-map-sequence-id-348205

    Enlarge

  16. pGEX6P1-N-HA

    Plasmid
    #119756
    Purpose
    Allows cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained N-terminal HA tag.
    Depositor
    Martin Reijns, Andrew Jackson
    Type
    Empty backbone
    Use
    Tags
    GST and HA
    Expression
    Bacterial
    Mutation
    Promoter
    tac promoter
    Available since
    Jan. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    119756-plasmid-map-sequence-id-233665

    Enlarge

  20. AAV2 for Split-PE, gRNAs + MMLV-RT(dRH)-P2A-eGFP (PKC1002)

    Plasmid
    #190112
    Purpose
    AAV2 for expression of pegRNA and ngRNA (HEKs3, CTT insertion) + MMLV-RT(dRH)-P2A-eGFP
    Depositor
    Keith Joung
    Article
    Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.
    Inserts
    MMLV-RT(dRH)
    U6-pegRNA(HEKs3, CTT ins)-H1-ngRNA(HEK s3)
    Use
    AAV
    Tags
    bpNLS and bpNLS-P2A-eGFP
    Expression
    Mutation
    mutations from RT in PE2 and truncation of RNAse …
    Promoter
    EFS and U6, H1
    Available since
    Sept. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    190112-plasmid-map-sequence-id-372717

    Enlarge

  21. R777-E285 Hs.ROCK2

    Plasmid
    #70569
    Purpose
    Gateway ORF clone of human ROCK2 [NM_004850.3] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    ROCK2 (ROCK2 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70569-plasmid-map-sequence-id-126018

    Enlarge

  22. pAAV.hSyn.iGluSnFR3.v857.GPI

    Plasmid
    #178331
    Purpose
    Fluorescent reporter for glutamate, third generation, variant 857 in GPI backbone. iGluSnFR3.v857.GPI
    Depositor
    Kaspar Podgorski
    Article
    Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4
    Has Service
    AAV1
    Insert
    pAAV hSyn iGluSnFR3 v857.GPI
    Use
    AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…
    Tags
    GPI Anchor, IgK-chain, and Myc epi tag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    178331-plasmid-map-sequence-id-361545

    Enlarge

  31. TFORF1307

    Plasmid
    #142371
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    JARID2 (JARID2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    142371-plasmid-map-sequence-id-397179

    Enlarge

  33. eGFP-hCOL1A1

    Plasmid
    #140110
    Purpose
    Expresses fluorescently-tagged human Type I procollagen α1 chain with eGFP replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage site
    Depositor
    Sergey Leikin
    Insert
    Type I procollagen α1 (COL1A1 Human)
    Use
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    COL1A1 exons 2-3 replaced by fluorescent tag, K20…
    Promoter
    CMV
    Available since
    May 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    140110-plasmid-map-sequence-id-276323

    Enlarge

  34. pSVL(D2m)

    Plasmid
    #29336
    Depositor
    John Taylor
    Article
    Kuo et al J Virol. 1989 May . 63(5):1945-50.
    Insert
    Dimer of HDV genome with 2 nucleotide deletion in delta antigen
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Contains a dimer of the complete HDV genome excep…
    Promoter
    Available since
    May 17, 2011
    Availability
    Academic Institutions and Nonprofits only
    29336-plasmid-map-sequence-id-394295

    Enlarge

  36. FUGW-APOE-E3-T2A-mCherry

    Plasmid
    #190808
    Purpose
    Express APOE E3 allele from Ubiquitin C promoter, co-expressing mCherry
    Depositor
    Ronald Hart
    Insert
    APOE (APOE Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    E3
    Promoter
    Ubi C
    Available since
    Sept. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    190808-plasmid-map-sequence-id-372853

    Enlarge

  37. FUGW-APOE-E4-T2A-mCherry

    Plasmid
    #190809
    Purpose
    Express APOE E4 allele from Ubiquitin C promoter, co-expressing mCherry
    Depositor
    Ronald Hart
    Insert
    APOE (APOE Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    E4
    Promoter
    Ubi C
    Available since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    190809-plasmid-map-sequence-id-374463

    Enlarge

  39. pET15b-ACPs (from S. pneumonia)

    Plasmid
    #63687
    Purpose
    Inducible expression of Acyl Carrier Protein Synthase (ACPs) from S. pneumoniae
    Depositor
    Michael Johnson
    Insert
    acpS
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only

  46. pCMV-T7-SpCas9-P2A-EGFP (RTW3027)

    Plasmid
    #139987
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpCas9 with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized SpCas9 with BPNLS-3xFLAG-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV and T7
    Available since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    139987-plasmid-map-sequence-id-275335

    Enlarge

  49. STARR-seq luciferase validation vector_ORI_CMV

    Plasmid
    #99312
    Purpose
    Luciferase validation vector with CMV enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activity
    Depositor
    Alexander Stark
    Article
    Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453
    Insert
    CMV enhancer
    Use
    Luciferase; Starr-seq luciferase validation vector
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 17, 2017
    Availability
    Academic Institutions and Nonprofits only
    99312-plasmid-map-sequence-id-190933

    Enlarge

Showing: 6451 - 6500 of 157653 results