159,332 results

1. pAAVS1-PDi-CRISPRn

   Plasmid

   #73500

   Purpose

   Dox-inducible CRISPR nuclease (CRISPRn) knock in construct into the AAVS1 locus

   Depositor

   Bruce Conklin

   Article

   Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

   Inserts

   Cas9
   rtTA
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CAG and TRE

   Available since

   Dec. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. C1(1-29)-TurboID-V5_pLX304

   Plasmid

   #107175

   Purpose

   expresses V5-tagged TurboID on the mammalian ER membrane, lentiviral vector

   Depositor

   Alice Ting

   Article

   Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

   Insert

   TurboID (BirA mutant)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

   Promoter

   Available since

   May 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSFFV_sfCherry2(1-10)

   Plasmid

   #82603

   Purpose

   Lentiviral vector for constitutive expression of sfCherry2(1-10)

   Depositor

   Bo Huang

   Article

   Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

   Insert

   sfCherry2(1-10)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   changed Glu 118 to Gln, changed Thr 128 to Ile

   Promoter

   SFFV

   Available since

   Jan. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pZac2.1-GfaABC1D-TurboID(full)-HA-GPI

   Plasmid

   #166055

   Purpose

   Expresses the full length TurboID on the surface of astrocytes

   Depositor

   Scott Soderling

   Article

   Takano et al Nature. 2020 Dec;588(7837):296-302. doi:

   Insert

   GPI anchored TurboID
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcsII-pb5-flag

   Plasmid

   #86769

   Purpose

   C-terminal flag-tagged protein expression in mammalian cells, lentiviral vector

   Depositor

   Do-Hyung Kim

   Article

   Yun et al Mol Cell. 2016 Feb 18;61(4):625-39. doi:

   Insert

   proteasome beta subunit 5 (PSMB5 Human)
   

   Use

   Lentiviral

   Tags

   flag

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available since

   Jan. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCRISPR-Cas3

   Plasmid

   #209427

   Purpose

   Plasmid for CASCADE-Cas3 based genome engineering of streptomycetes

   Depositor

   Tilmann Weber

   Article

   Whitford et al Nucleic Acids Res. 2025 Mar 20;53(6):gka

   Insert

   codon optimized minimal type I-C CASCADE-Cas3 from Pseudomonas aeruginosa
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   PtipA; crRNA under control of PermE*

   Available since

   March 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. mCherry-SEpHluorin

   Plasmid

   #32001

   Depositor

   Sergio Grinstein

   Article

   Koivusalo et al J Cell Biol. 2010 Feb 22;188(4):547-63.

   Insert

   mCherry
   

   Use

   Tags

   Superecliptic pHluorin

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 9, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAVdual-CMV-eGFP

   Plasmid

   #230931

   Purpose

   pAAVdual plasmid is used to package AAV-CMV-eGFP viruses with the AAVdual system, which integrates the mini-pHelper-1.0 (providing E2A, E4orf6, and VA RNA) with an AAV expression cassette containing eGFP driven by the CMV promoter.

   Depositor

   Qizhao Wang

   Insert

   eGFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pB80-hKif5b(1-560)rigor-2xmNeongreen

   Plasmid

   #174649

   Purpose

   Live-cell marker for stable microtubules

   Depositor

   Lukas Kapitein

   Article

   Jansen et al J Cell Biol. 2023 May 1;222(5):e20210610

   Insert

   hKif5B(1-560)rigor-2xmNeongreen (KIF5B Human)
   

   Use

   Tags

   mNeongreen-mNeongreen

   Expression

   Mammalian

   Mutation

   rigor mutation G234A

   Promoter

   Chicken beta-actin

   Available since

   March 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKIBra-P2A-mCherry-Puro-sfGFP

    Plasmid

    #115686

    Purpose

    Knock-in of P2A-mCherry at the mouse Brachyury (T) gene

    Depositor

    David Suter

    Article

    Deluz et al Genes Dev. 2016 Nov 15;30(22):2538-2550.

    Insert

    P2A-mCherry-loxP-PGK-Puro-sfGFP-loxP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJT300 Lenti pEF-dCas9-3XFLAG-ZNF705_KRAB-T2A-tagBFP-P2A-Blast

    Plasmid

    #195542

    Purpose

    dCas9 effector fusion for manipulating transcription

    Depositor

    Lacra Bintu, Michael Bassik

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    Lenti pEF-dCas9-3XFLAG-ZNF705_KRAB-T2A-tagBFP-P2A-Blast
    

    Use

    CRISPR and Lentiviral

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    pEF1a

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. RepX_pET28a(+)

    Plasmid

    #172199

    Purpose

    Expresses 6xHis-tagged RepX in E. coli BL21(DE3), kanamycin resistance, optimal growth at 20 degrees.

    Depositor

    Taekjip Ha

    Article

    Arslan et al Science. 2015 Apr 17;348(6232):344-7. do

    Insert

    RepX superhelicase
    

    Use

    Tags

    6xHis tag

    Expression

    Bacterial

    Mutation

    C18L, C43S, C167V, S400C, C612A

    Promoter

    Available since

    March 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-HA2-ROC1

    Plasmid

    #19897

    Depositor

    Yue Xiong

    Article

    Ohta et al Mol Cell. 1999 Apr . 3(4):535-41.

    Insert

    RING finger protein 1 (ROC1) (RBX1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 31, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. gRNA_Cloning Vector

    Plasmid

    #41824

    Purpose

    An empty gRNA expression vector, used to create a gRNA to a specific sequence.

    Depositor

    George Church

    Article

    Mali et al Science. 2013 Feb 15;339(6121):823-6. do

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    AflII site for Gibson Assembly

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-FlipCherry-T2A-GFP

    Plasmid

    #124436

    Purpose

    Expresses FlipCherry (TEV cleavage sequence) and T2A GFP in mammalian cells

    Depositor

    Xiaokun Shu

    Article

    Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

    Insert

    FlipCherry-T2A-GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. Fuw-dCas9-Dnmt3a

    Plasmid

    #84476

    Purpose

    Lentiviral construct to express dCas9 fused with Dnmt3a

    Depositor

    Rudolf Jaenisch

    Article

    Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

    Insert

    dCas9-Dnmt3a (DNMT3A Synthetic, Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pmRFP_LAP2beta_IRES_puro2b

    Plasmid

    #21047

    Depositor

    Daniel Gerlich

    Article

    Steigemann et al Cell. 2009 Feb 6. 136(3):473-84.

    Insert

    LAP2_beta (TMPO Rat)
    

    Use

    Tags

    mRFP

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    July 14, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. M43: pHR-SFFVp-iLID::EGFP::FTH1

    Plasmid

    #122149

    Purpose

    Self-assebled 24-mer tagged with iLID, and EGFP. Upon blue-light activation can bind up to 24 SspB-modified proteins.

    Depositor

    Clifford Brangwynne

    Article

    Bracha et al Cell. 2018 Nov 29;175(6):1467-1480.e13.

    Insert

    FTH1 (FTH1 Human)
    

    Use

    Lentiviral

    Tags

    NLS-iLID-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    March 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. FUGW-Cytosol-TurboID

    Plasmid

    #200961

    Purpose

    3rd gen lentiviral plasmid for expressing cytosol localized TurboID with EGFP

    Depositor

    Keriann Backus

    Article

    Yan et al Cell Chem Biol. 2023 Jul 20;30(7):811-82

    Insert

    Cytosol-TurboID
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-CAG-H2B tdTomato

    Plasmid

    #116870

    Purpose

    Nuclear localized tdTomato

    Depositor

    Loren Looger

    Article

    Economo et al Nature. 2018 Nov;563(7729):79-84. doi: 1

    Insert

    H2B tdTomato
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pFUW-tetO-Bcl11a

    Plasmid

    #139809

    Purpose

    doxycycline-inducible expression of human Bcl11a in mammalian cells

    Depositor

    Filipe Pereira

    Article

    Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

    Insert

    BCL11a (BCL11A Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE-mCMV

    Available since

    Sept. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. TFORF1152

    Plasmid

    #142739

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    MYOCD (MYOCD Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. phage UbiC NLS HA stdMCP stdHalo

    Plasmid

    #104999

    Purpose

    Lentiviral vector expressing synonymous tandem MS2 coat protein fused to synonymous tandem Halo-tag for mRNA labeling

    Depositor

    Jeffrey Chao

    Article

    Voigt et al Cell Rep. 2017 Dec 26;21(13):3740-3753.

    Insert

    stdMCP-stdHalo
    

    Use

    Lentiviral

    Tags

    HA tag, NLS, Two synonymous copies of the Halo-ta…

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    UbiC

    Available since

    Feb. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCMV-ABCB10-ChR2-eYFP

    Plasmid

    #206037

    Purpose

    Express ChR2 in mitochondria

    Depositor

    Lufang Zhou

    Article

    Ernst et al Biophys J. 2019 Aug 20;117(4):631-645. d

    Insert

    ChR2
    

    Use

    Tags

    ABCB10 mitochondrial leading sequence and EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCDNA 3.1 Flag-rat-PTP1B

    Plasmid

    #35988

    Depositor

    Anna Huttenlocher

    Article

    Cortesio et al J Cell Biol. 2008 Mar 10;180(5):957-71.

    Insert

    protein tyrosine phosphatase 1B (Ptpn1 Rat)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 1, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pSerOTS-C1* (V70)

    Plasmid

    #188537

    Purpose

    Phosphoserine incorporation into recombinant proteins

    Depositor

    Jesse Rinehart

    Article

    Mohler et al Mol Syst Biol. 2023 Aug 8;19(8):e10591.

    Inserts

    pSerRS
    Ef-pSer
    tRNApSer
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    mutations resulting from laboratory evolution

    Promoter

    glnS*

    Available since

    Dec. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pHIV-H2B-eGFP

    Plasmid

    #91776

    Purpose

    Expresses H2B protein tagged with eGFP

    Depositor

    Maria Pia Cosma

    Article

    Sottile et al Sci Rep. 2016 Nov 9;6:36863. doi: 10.103

    Insert

    Human Histone H2B / eGFP (H2BC11 Human)
    

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. PBCAG-eGFP

    Plasmid

    #40973

    Depositor

    Joseph Loturco

    Article

    Chen et al J Neurosci Methods. 2012 Jun 15;207(2):1

    Inserts

    PiggyBac transposon 3' terminal repeats
    PiggyBac 5' terminal repeats
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. p101_CRISPRai_PiggyBac

    Plasmid

    #213777

    Purpose

    Expresses Tet-On inducible CRISPRai orthogonal system with VPR-dSaCas9, dSpCas9-KRAB-BFP, zeocin resistance gene, and Tet-On transactivator. PiggyBac vector.

    Depositor

    Howard Chang

    Article

    Pacalin et al Nat Biotechnol. 2024 May 17. doi: 10.103

    Inserts

    VPR-dSaCas9
    dSpCas9-KRAB-BFP
    zeocin
    Tet-On transactivator
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    EF1a (EF-1-alpha intron a) and Tet_On_TRE

    Available since

    May 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. LeGO-mOrange2

    Plasmid

    #85212

    Purpose

    Lentiviral vector expressing mOrange2 fluorescent protein driven by SFFV promoter

    Depositor

    Boris Fehse

    Article

    Mohme et al Mol Ther. 2017 Jan 18. pii: S1525-0016(1

    Insert

    mOrange2
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    Jan. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pYS19

    Plasmid

    #202084

    Purpose

    Frame -2 selector for NHEJ knock-in

    Depositor

    Daniel Dickinson

    Article

    Shi et al Development. 2023 May 15;150(10):dev2013

    Insert

    pEF1a-mCherry-Cas9 + Frame –2 sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. SpCas9-ABE8e(V106W)

    Plasmid

    #198547

    Purpose

    Expresses human codon-optimized SpCas9-ABE8e(V106W) and blasticidin resistance: EFS promoter-SpCas9-ABE8e(V106W)-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

    Insert

    SpCas9-ABE8e(V106W)
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pLAMP1-emiRFP670

    Plasmid

    #136570

    Purpose

    LAMP1 (Lysosome Membrane) labeling with near-infrared fluorescent protein emiRFP670 (enhanced miRFP670)

    Depositor

    Vladislav Verkhusha

    Article

    Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

    Insert

    LAMP1-emiRFP670
    

    Use

    Tags

    emiRFP670

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pEMG-GRIS

    Plasmid

    #225134

    Purpose

    For genome editing in P. putida KT2440, rhamnose is used for the induction of I-sceI gene. Gm.

    Depositor

    Lars Blank

    Article

    Meng et al Microb Biotechnol. 2024 Jul;17(7):e14531

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 10, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

35. BE4-Gam

    Plasmid

    #100806

    Purpose

    Expresses BE4-Gam in mammalian cells

    Depositor

    David Liu

    Article

    Komor et al Sci Adv. 2017 Aug 30;3(8):eaao4774. doi:

    Insert

    BE4-Gam
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pdCas9-humanized

    Plasmid

    #44246

    Purpose

    Expression of a catalytically inactive, human codon-optimized Cas9 under the control of Murine Stem Cell retroVirus LTR promoter for mammalian gene knockdown

    Depositor

    Stanley Qi

    Article

    Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1

    Insert

    dead Cas9 with 3X NLS
    

    Use

    CRISPR and Retroviral; Vector

    Tags

    3x NLS

    Expression

    Mammalian

    Mutation

    D10A H840A (catalytically inactive)

    Promoter

    MSCV LTR promoter

    Available since

    April 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pME-nfsB-CFP

    Plasmid

    #86374

    Purpose

    expression of nfsB-CFP

    Depositor

    Rachel Wong

    Article

    Yoshimatsu et al Nat Commun. 2016 Feb 3;7:10590. doi: 10.

    Insert

    nitroreductase
    

    Use

    Zebrafish

    Tags

    ECFP

    Expression

    Mutation

    Promoter

    Available since

    March 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. TFORF2069

    Plasmid

    #141902

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    MYC (MYC Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pET His6 GFP TEV LIC cloning vector (1GFP)

    Plasmid

    #29663

    Depositor

    Scott Gradia

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Tags

    6xHis, GFP, and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available since

    June 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pEGFPC1-hVAP-A

    Plasmid

    #104447

    Purpose

    Expression of human VAP-A fused to GFP in mammalian cells

    Depositor

    Catherine Tomasetto

    Article

    Alpy et al J Cell Sci. 2013 Dec 1;126(Pt 23):5500-1

    Insert

    vesicle-associated membrane protein-associated protein A (VAPA Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pCAS

    Plasmid

    #60847

    Purpose

    Expresses S. pyogenes Cas9 plus an HDV ribozyme-sgRNA for genome editing in yeast

    Depositor

    Jamie Cate

    Article

    Ryan et al Elife. 2014 Aug 19;3. doi: 10.7554/eLife

    Inserts

    S. pyogenese Cas9
    RNA pol III promoter (tRNA-Tyr)
    hepatitis delta virus ribozyme, genomic
    sgRNA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    NLS/His8 and TTT 3' extension prior to sgRNA

    Expression

    Bacterial and Yeast

    Mutation

    L4 is UUCG tetraloop and guide targets LYP1 (CATA…

    Promoter

    Available since

    Jan. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pcDNA3.1/Puro-CAG-ASAP1

    Plasmid

    #52519

    Purpose

    For mammalian expression of the fluorescent voltage sensor ASAP1

    Depositor

    Michael Lin

    Article

    St-Pierre et al Nat Neurosci. 2014 Apr 22. doi: 10.1038/

    Insert

    Accelerated Sensor of Action Potentials 1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. HA-Halotag-FRB-EX-nes_pLX304

    Plasmid

    #120913

    Purpose

    Lentiviral vector expressing HA-Halotag-FRB-EX-NES in mammalian cytosol

    Depositor

    Alice Ting

    Article

    Han et al ACS Chem Biol. 2019 Mar 8. doi: 10.1021/

    Insert

    HA-Halotag-FRB-EX-NES
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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44. pHR-CD28-mCherry

    Plasmid

    #223593

    Purpose

    Lentiviral expression of human CD28 with mCherry tag in mammalian cells

    Depositor

    Enfu Hui

    Article

    Zhao et al Immunity. 2019 Dec 17;51(6):1059-1073.e9

    Insert

    CD28 (CD28 Human)
    

    Use

    Lentiviral; mammalian expression

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    Available since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. MZB209

    Plasmid

    #228492

    Purpose

    BirA encoded pACYC vector for co-expression with Avi-tagged constructs (Bacterial Expression)

    Depositor

    Richard Baker

    Insert

    BirA (birA )
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available since

    Jan. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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46. pBBR1.cas9-NT

    Plasmid

    #197871

    Purpose

    Constitutive expression of Cas9 endonuclease, trancRNA and crRNA with a randomly generated spacer sequence (non-targeting)

    Depositor

    Baojun Wang

    Article

    Huan et al ACS Synth Biol. 2023 Mar 17;12(3):709-72

    Inserts

    trancCRNA
    Cas9
    crRNA (non-chromosomal (E. coli/S. flexneri)-targeting spacer sequence)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. VAMP2-pHmScarlet

    Plasmid

    #166890

    Purpose

    For vesicle exocytosis visualization using pHmScarlet

    Depositor

    Pingyong Xu

    Article

    Liu et al Nat Commun. 2021 Mar 3;12(1):1413. doi:

    Insert

    VAMP2 (Vamp2 Mouse)
    

    Use

    Tags

    pHmScarlet

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pTRE3G-EBF1

    Plasmid

    #96965

    Purpose

    Dox-inducible expression of Ebf1 in mammalian cells

    Depositor

    Elena Cattaneo

    Article

    Faedo et al Proc Natl Acad Sci U S A. 2017 Feb 14;11

    Insert

    Ebf1 (Ebf1 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pMRX-IB-TMEM192-mKeima

    Plasmid

    #215359

    Purpose

    Probe to evaluate lysophagic activity

    Depositor

    Tamotsu Yoshimori

    Article

    Shima et al J Cell Biol. 2023 Dec 4;222(12):e2022040

    Insert

    transmembrane protein 192 (TMEM192 Human)
    

    Use

    Retroviral

    Tags

    mKeima

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pCAS9i

    Plasmid

    #141249

    Purpose

    Galactose-inducible expression of Cas9 for addressing one target; Contains guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.

    Depositor

    Adam Bertl

    Article

    Degreif et al J Biol Methods. 2018 Sep 12;5(3):e98. do

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1 (Cas9), pSNR52 (gRNA)

    Available since

    Aug. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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