19,178 results

1. pLNHA-C1-HsXRN1

   Plasmid

   #66596

   Purpose

   expresses lambdaN-HA-tagged XRN1 in mammalian cells

   Depositor

   Elisa Izaurralde

   Article

   Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

   Insert

   XRN1 (XRN1 Human)
   

   Use

   Tags

   lambdaN-HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLEGFP-S727A-STAT3

   Plasmid

   #71446

   Purpose

   Retroviral expression of S727A-STAT3. Please note that this plasmid contains S727A-STAT3 tagged with FLAG, not GFP.

   Depositor

   George Stark

   Article

   Dasgupta et al Proc Natl Acad Sci U S A. 2015 Mar 31;11

   Insert

   STAT3 (STAT3 Human)
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mutation

   S727A

   Promoter

   CMV

   Available since

   Nov. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pIRESneo-EGFP-alpha Tubulin

   Plasmid

   #12298

   Depositor

   Patricia Wadsworth

   Article

   Rusan et al Mol Biol Cell. 2001 Apr . 12(4):971-80.

   Insert

   EGFP-alpha Tubulin (TUBA1B Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 11, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCMV 3xFLAG-LC3B WT

   Plasmid

   #123092

   Purpose

   Expresses 3xFLAG-LC3B wild-type in mammalian cells.

   Depositor

   Robin Ketteler

   Article

   Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

   Insert

   Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3.3_SOX2

   Plasmid

   #26817

   Depositor

   Derrick Rossi

   Article

   Warren et al Cell Stem Cell. 2010 Sep 29. ():.

   Insert

   5'UTR-SOX2-3'UTR (SOX2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 17, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. pCFJ1137

   Plasmid

   #84827

   Purpose

   Minimos transposon with Peft-3:GFP:tbb-2 3'UTR and cbr-unc-119 selection. GFP was optimized for C. elegans, contains 2x NLS (SV40/egl-13), and synthetic introns

   Depositor

   Andrew Fire

   Article

   Frokjaer-Jensen et al Cell. 2016 Jul 14;166(2):343-57. doi: 10

   Insert

   C. elegans codon optimized GFP
   

   Use

   Tags

   Expression

   Bacterial and Worm

   Mutation

   Promoter

   Peft-3

   Available since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. EGFP-hAgo2

   Plasmid

   #21981

   Depositor

   Phil Sharp

   Article

   Leung et al Proc Natl Acad Sci U S A. 2006 Nov 28. 1

   Insert

   Argonaute 2 (AGO2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 21, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pSIN-PAmCherry-mSNCA-NE

   Plasmid

   #102364

   Purpose

   Lentiviral overexpression of PA-mCherry synuclein alpha fusion

   Depositor

   Shu Leong Ho

   Article

   Ho et al Autophagy. 2019 Apr 14:1-24. doi: 10.108

   Inserts

   PA-mCherry
   synuclein alpha (Snca Mouse)
   

   Use

   Lentiviral

   Tags

   NE

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available since

   April 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3.2 TDP-43 NLS1 YFP

   Plasmid

   #84912

   Purpose

   Mammalian expresion of TDP-43 NLS1 YFP

   Depositor

   Aaron Gitler

   Article

   Elden et al Nature. 2010 Aug 26;466(7310):1069-75. d

   Insert

   TDP-43 (TARDBP Human)
   

   Use

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   K82A, R83A, K84A

   Promoter

   CMV

   Available since

   Nov. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. IMS-APEX2

    Plasmid

    #79058

    Purpose

    Expresses APEX in the mitochondrial intermembrane space.

    Depositor

    Alice Ting

    Article

    Hung et al Mol Cell. 2014 Jul 17;55(2):332-41. doi:

    Insert

    APEX
    

    Use

    Tags

    V5

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    T7 promoter

    Available since

    Aug. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pGWB501

    Plasmid

    #74843

    Purpose

    Gateway cloning compatible binary vector for simple cloning of gene.

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    No Promoter

    Available since

    Aug. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. AP2u2-mCherry

    Plasmid

    #27672

    Depositor

    Christien Merrifield

    Article

    Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

    Insert

    Ap2m1 (Ap2m1 Rat)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 4, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pGEX-4T1 human PP2A Aα full length

    Plasmid

    #132634

    Purpose

    expresses human PP2A Aα full length in E. coli

    Depositor

    Wenqing Xu

    Article

    Sangodkar et al J Clin Invest. 2017 Jun 1;127(6):2081-20

    Insert

    PP2A Aα (PPP2R1A Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pNICE-NL2A

    Plasmid

    #15259

    Depositor

    Peter Scheiffele

    Article

    Chih et al Neuron. 2006 Jul 20. 51(2):171-8.

    Insert

    Neuroligin 2 with insert A (Nlgn2 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCAG HRP-TM

    Plasmid

    #44441

    Depositor

    Alice Ting

    Article

    Rhee et al Science. 2013 Jan 31.

    Insert

    Horseradish peroxidase fused to the transmembrane domain of PDGFR
    

    Use

    Tags

    HA tag and myc tag

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCas9D10A_GFP

    Plasmid

    #44720

    Purpose

    Co-expression of human codon-optimized Cas9 (D10A) mutant nickase and GFP, plasmid optimized for expression in human pluripotent stem cells

    Depositor

    Kiran Musunuru

    Article

    Ding et al Cell Stem Cell. 2013 Apr 4;12(4):393-4.

    Insert

    Cas9D10A-2A-GFP
    

    Use

    CRISPR

    Tags

    2A-GFP

    Expression

    Mammalian

    Mutation

    D10A mutation- nickase mutant

    Promoter

    CAG

    Available since

    May 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDAS12489_PEAR-GFP_2in1_2.0

    Plasmid

    #177186

    Purpose

    PEAR-GFP3 plasmid with a pegRNA that targets its own plasmid

    Depositor

    Ervin Welker

    Article

    Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

    Inserts

    EGFP split with an intron between amino acids 95-96
    pegRNA targeting its own plasmid
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    disrupted 5' splice site

    Promoter

    CMV and U6

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pnsgRNA

    Plasmid

    #172717

    Purpose

    Delivery of guide RNA for prime editing

    Depositor

    Tilmann Weber

    Article

    Tong et al Nat Commun. 2021 Sep 1;12(1):5206. doi:

    Insert

    J23119-gRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pFRT-TODestFLAGHAhFMRPiso1

    Plasmid

    #48690

    Purpose

    Expresses hFMRP iso 1

    Depositor

    Thomas Tuschl

    Article

    Ascano M et al Nature. 2012 Dec 20;492(7429):382-6. doi

    Insert

    FlagHA FMR1 Iso 1 (FMR1 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, 2x Tet

    Available since

    May 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. EGFP-Rab5A Q79L

    Plasmid

    #28046

    Depositor

    Qing Zhong

    Article

    Sun et al Proc Natl Acad Sci U S A. 2010 Nov 9. 10

    Insert

    Rab5A (RAB5A Human)
    

    Use

    Tags

    EGFP on N-term

    Expression

    Mammalian

    Mutation

    Q79L

    Promoter

    Available since

    Feb. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. HTR1A-Tango

    Plasmid

    #66404

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    HTR1A (HTR1A Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. mCherry-Mem

    Plasmid

    #55779

    Purpose

    This red fluorescent plasma membrane marker consists of the first twenty residues of neuromodulin fused to mCherry.

    Depositor

    Catherine Berlot

    Article

    Yost et al Mol Pharmacol. 2007 Oct;72(4):812-25. Ep

    Insert

    mCherry-Mem
    

    Use

    Tags

    The N-terminal 20 residues of neuromodulin are fu…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pENTR5'_ubi

    Plasmid

    #27320

    Depositor

    Leonard Zon

    Article

    Mosimann et al Development. 2011 Jan . 138(1):169-77.

    Insert

    zebrafish ubiquitin promoter (ubb Zebrafish)
    

    Use

    Multisite gateway 5' entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 3, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCLXSN

    Plasmid

    #12343

    Depositor

    Inder Verma

    Article

    Naviaux et al J Virol. 1996 Aug . 70(8):5701-5.

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. DYRK1A/pTRE3G-FL-hXIST

    Plasmid

    #149608

    Purpose

    Express doxycycline-inducible full length of human XIST cDNA

    Depositor

    Jeanne Lawrence

    Article

    Jiang et al Nature. 2013 Aug 15;500(7462):296-300. d

    Insert

    X-inactive specific transcript (XIST Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pTRE3G

    Available since

    July 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. HA-Elongin B-pcDNA3.1(+)-Zeo

    Plasmid

    #20000

    Depositor

    William Kaelin

    Article

    Ohh et al Nat Cell Biol. 2000 Jul . 2(7):423-7.

    Insert

    transcription elongation factor B (ELOB Human)
    

    Use

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    Available since

    April 6, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pRBC-sfGFP wild type

    Plasmid

    #174075

    Purpose

    Plasmid with p15a origin of replication expressing codon optimized superfolder GFP with C-terminal His6 tag, under T7 promoter

    Depositor

    Ryan Mehl

    Article

    Zhu et al ACS Chem Biol. 2019 Jul 19;14(7):1564-15

    Insert

    Superfolder GFP
    

    Use

    Tags

    His-6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCI SMN2

    Plasmid

    #72287

    Purpose

    SMN2 exon 7 splicing cassette

    Depositor

    Elliot Androphy

    Article

    Lorson et al Proc Natl Acad Sci U S A. 1999 May 25;96

    Insert

    SMN2 (SMN2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    contains exons 6-7-8 including introns, multiple …

    Promoter

    CMV

    Available since

    Jan. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAAV-Ef1a-DIO eNpHR 3.0-EYFP

    Plasmid

    #26966

    Purpose

    AAV expression of eNpHR 3.0 fused to EYFP driven by EF1a promoter for optogenetic inhibition

    Depositor

    Karl Deisseroth

    Article

    Gradinaru et al Cell. 2010 Apr 2. 141(1):154-65.

    Has Service

    AAV Retrograde, AAV1, AAV5, and AAV9

    Insert

    eNpHR 3.0
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    ER export at 3' end of gene. Trafficking sig…

    Promoter

    EF1alpha

    Available since

    Jan. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. myrAkt delta4-129

    Plasmid

    #10841

    Depositor

    Richard Roth

    Article

    Kohn et al J Biol Chem. 1996 Sep 6. 271(36):21920-6

    Insert

    Akt (AKT1 Human)
    

    Use

    Tags

    HA and myr

    Expression

    Mammalian

    Mutation

    Deletion of PH domain (aa4-129)

    Promoter

    SV40

    Available since

    Feb. 13, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. mCardinal-C1

    Plasmid

    #54799

    Purpose

    Localization: C1 Cloning Vector, Excitation: 604, Emission: 659

    Depositor

    Michael Davidson

    Article

    Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

    Type

    Empty backbone

    Use

    Tags

    mCardinal

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

32. pBA900

    Plasmid

    #122237

    Purpose

    Lentiviral CRISPR guide vector expressing a eGFP-NT2 sgRNA with cs2 incorporated at the 3' end of the sgRNA constant region.

    Depositor

    Jonathan Weissman

    Article

    Replogle et al Nat Biotechnol. 2020 Aug;38(8):954-961.

    Insert

    sgRNA with cs2 at the 3' end of the constant region
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pKLV2-U6gRNA5(gGFP)-PGKmCherry2AGFP-W

    Plasmid

    #67982

    Purpose

    Cas9 activity reporter with mCherry and GFP

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    U6gRNA cassette, PGKmCherry2AGFP cassette, WPRE
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Deleted BbsI site within WPRE

    Promoter

    Available since

    Nov. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pcDNA3-mNeptune2-N

    Plasmid

    #41645

    Purpose

    Expresses far-red FP mNeptune2 fused to Dronpa145N. Dronpa145N oligomerizes under violet light (390-400nm) and dissociates under cyan light (490-500nm)

    Depositor

    Michael Lin

    Article

    Zhou et al Science. 2012 Nov 9;338(6108):810-4. doi

    Insert

    mNeptune2-Dronpa145N
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    K145N on Dronpa (allows control of photochromic F…

    Promoter

    CMV

    Available since

    Dec. 21, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. CMV500 A-CREB

    Plasmid

    #33371

    Depositor

    Charles Vinson

    Article

    Ahn et al Mol Cell Biol. 1998 Feb . 18(2):967-77.

    Insert

    CREB (Creb1 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Dominant Negative (see notes)

    Promoter

    CMV

    Available since

    Dec. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. GFP-OMP25

    Plasmid

    #141150

    Purpose

    Fluorescent labeling of the outer mitochondrial membrane (OMM) in mammalian cells

    Depositor

    Gia Voeltz

    Article

    Abrisch et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

    Insert

    OMP25 (Synj2bp Rat)
    

    Use

    Tags

    AcGFP

    Expression

    Mammalian

    Mutation

    OMP25 c terminus from addgene plasmid #69598

    Promoter

    CMV

    Available since

    Aug. 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCMV-STAT6-IRES-Neo

    Plasmid

    #35482

    Depositor

    Axel Nohturfft

    Insert

    STAT6 (STAT6 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pSR59.4

    Plasmid

    #63175

    Purpose

    Expresses OmpR constitutively from PompB97 and sfGFP under the PompF146 promoter

    Depositor

    Jeffrey Tabor

    Article

    Schmidl et al ACS Synth Biol. 2014 Nov 21;3(11):820-31

    Inserts

    OmpR (ompR )
    sfGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pYLCRISPR/Cas9P35S-N

    Plasmid

    #66191

    Purpose

    expression of Cas9 in plants, cauliflower mosaic virus 35S promoter (P35S), Neo selection

    Depositor

    Yao-Guang Liu

    Article

    Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

    Insert

    Cas9
    

    Use

    Tags

    Expression

    Plant

    Mutation

    plant-codon optimized with higher GC contents (62…

    Promoter

    cauliflower mosaic virus 35S promoter (P35S)

    Available since

    Oct. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pTiGc

    Plasmid

    #78314

    Purpose

    Mycobacterial dual reporter plasmid for fluorescence dilution. Description: hsp60-gfp, hsp60(ribo)-turboFP635 (inducible TurboFP635 under control of theophylline - inducible riboswitch).

    Depositor

    Samantha Sampson

    Article

    Mouton et al Microbiology. 2016 Mar 30. doi: 10.1099/

    Inserts

    TurboFP635
    GFP
    

    Use

    Synthetic Biology; Mycobacterial replicating vect…

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    hsp60 and hsp60(ribo)

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

41. CS2 Flag-Smad6

    Plasmid

    #14961

    Depositor

    Joan Massague

    Article

    Hata et al Genes Dev. 1998 Jan 15. 12(2):186-97.

    Insert

    Smad6 (SMAD6 Human)
    

    Use

    Xenopus/mammalian/avian/zebrafish

    Tags

    Flag

    Expression

    Mutation

    Promoter

    Available since

    May 17, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pIRESneo-PA-GFP-alpha Tubulin

    Plasmid

    #12297

    Depositor

    Patricia Wadsworth

    Article

    Tulu et al Curr Biol. 2003 Oct 28. 13(21):1894-9.

    Insert

    alpha Tubulin (TUBA1B Human)
    

    Use

    Tags

    PA-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 11, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. Flag-NRF1

    Plasmid

    #34707

    Depositor

    Raymond Deshaies

    Article

    Radhakrishnan et al Mol Cell. 2010 Apr 9;38(1):17-28.

    Insert

    NRF1 (NFE2L1 Human)
    

    Use

    Retroviral

    Tags

    3XFlag

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available since

    Feb. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

44. pcDNA5/FRT/TO V5 HSPA1A

    Plasmid

    #19510

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

    Insert

    heat shock 70kDa protein 1A (HSPA1A Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 7, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pXN22-Dest

    Plasmid

    #105085

    Purpose

    Yeast mating-based Split Ubiquitin Assay, prey vector for Gateway cloning

    Depositor

    Christopher Grefen

    Article

    Grefen et al Curr Protoc Neurosci. 2007 Oct;Chapter 5

    Type

    Empty backbone

    Use

    Tags

    NubG-3xHA

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. ELONGIN BC (XX01TCEB1A-c001)

    Plasmid

    #110274

    Purpose

    Elongin B and Elongin C co-expression plasmid for structure determination; Full length Elongin B & amino acids 17-112 of Elogin C

    Depositor

    Nicola Burgess-Brown

    Insert

    Elongin BC (ELOC, ELOB Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Elongin B is full length. Elongin C contains a.a…

    Promoter

    T7

    Available since

    May 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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47. CHOP promoter (-649/+136) pmCherry-1

    Plasmid

    #36035

    Depositor

    Quan Lu

    Article

    Oh et al J Biol Chem. 2012 Feb 17;287(8):6025-34.

    Insert

    CHOP promoter (-647 to +136) (DDIT3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 7, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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48. CMV500 A-FOS

    Plasmid

    #33353

    Depositor

    Charles Vinson

    Article

    Ahn et al Mol Cell Biol. 1998 Feb . 18(2):967-77.

    Insert

    FOS (Fos Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Dominant Negative of c-Jun; heterodimerizes and i…

    Promoter

    CMV

    Available since

    Dec. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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49. mNeonGreen-Rab7

    Plasmid

    #129603

    Purpose

    In vivo visualization of Rab7 (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Chertkova et al bioRxiv 160374

    Insert

    Rab7
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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50. Flag-PML IV/pRK5

    Plasmid

    #59742

    Purpose

    Expresses the isoform IV of Promyelocytic Leukemia Protein (PML) in mammalian cells

    Depositor

    Xiaolu Yang

    Article

    Guo et al Mol Cell. 2014 Jul 3;55(1):15-30. doi: 1

    Insert

    PML isoform IV (PML Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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