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We narrowed to 8,360 results for: construct

Showing: 6601 - 6650 of 8360 results

  4. c3GIC9n

    Plasmid
    #62192
    Purpose
    col1a1 targeting vector for inducible [TRE3G]-GFP-IRES-Cas9n (D10A variant) expression. Contains NsiI cloning site for U6-sgRNA cassettes
    Depositor
    Lukas Dow
    Article
    Dow et al Nat Biotechnol. 2015 Apr;33(4):390-4. do
    Insert
    humanized S. Pyogenes Cas9 (D10A)
    Use
    CRISPR
    Tags
    HA
    Expression
    Mutation
    D10A substitution (Cas9n)
    Promoter
    TRE3G
    Available since
    Aug. 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    62192-plasmid-map-sequence-id-156364

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  5. mOtop1_pGEMHE

    Plasmid
    #114674
    Purpose
    This construct was used to express mouse Otop1 in Xenopus laevis oocytes
    Depositor
    Emily Liman
    Article
    Tu et al Science. 2018 Mar 2;359(6379):1047-1050.
    Insert
    Otopetrin 1 (Otop1 Mouse)
    Use
    For generating the mrna, which can highly express…
    Tags
    Expression
    Mutation
    SNP (rs49325632): Glycine 396 to Alanine
    Promoter
    T7
    Available since
    July 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    114674-plasmid-map-sequence-id-226716

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  8. pX330_Actb sgRNA / hSpCas9

    Plasmid
    #172832
    Purpose
    Mammalian expression of a sgRNA targeting the intron 1of Actb (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.
    Depositor
    Haining Zhong
    Article
    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1
    Insert
    sgRNA targeting intron 1 of Actb under the U6 promotor and hSpCas9 under the CAG promotor
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    172832-plasmid-map-sequence-id-340914

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  13. pPonA-BI-Gl NORM-LacZA TER- LacZB

    Plasmid
    #86212
    Purpose
    beta Globin NMD construct with and without PTC under Bi-directional Ponasterone A inducible promoter
    Depositor
    Robert Singer
    Article
    Trcek et al Genes Dev. 2013 Mar 1;27(5):541-51. doi:
    Insert
    beta-Globin (HBB, Hbb Mouse, Human)
    Use
    Ponasterone a inducible promoter
    Tags
    lacZA or lacZB
    Expression
    Mammalian
    Mutation
    39TER in beta Globin in MCS II
    Promoter
    Ponasterone A inducible
    Available since
    Jan. 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    86212-plasmid-map-sequence-id-169958

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  14. pOPS0378

    Plasmid
    #133229
    Purpose
    Reporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.
    Depositor
    Philip Poole
    Article
    Mendoza-Suarez et al Proc Natl Acad Sci U S A. 2020 May 5;117
    Insert
    constructed with constitutive promoter pNeo (pOGG001), mCherry (EC15071) and T-pharma (pOGG003)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Domesticated for Golden Gate cloning
    Promoter
    Available since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    133229-plasmid-map-sequence-id-276542

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  25. pOPS0384

    Plasmid
    #133234
    Purpose
    Reporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.
    Depositor
    Philip Poole
    Article
    Mendoza-Suarez et al Proc Natl Acad Sci U S A. 2020 May 5;117
    Insert
    constructed with NoP (pOGG113), mCherry (EC15071) and T-pharma (pOGG003)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Domesticated for Golden Gate cloning
    Promoter
    Available since
    April 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    133234-plasmid-map-sequence-id-287535

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  28. pOPS0385

    Plasmid
    #133235
    Purpose
    Reporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.
    Depositor
    Philip Poole
    Article
    Mendoza-Suarez et al Proc Natl Acad Sci U S A. 2020 May 5;117
    Insert
    constructed with NoP (pOGG113), gusA (pOGG083) and T-pharma (pOGG003)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Domesticated for Golden Gate cloning
    Promoter
    Available since
    April 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    133235-plasmid-map-sequence-id-287549

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  29. pOPS0386

    Plasmid
    #133236
    Purpose
    Reporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.
    Depositor
    Philip Poole
    Article
    Mendoza-Suarez et al Proc Natl Acad Sci U S A. 2020 May 5;117
    Insert
    constructed with NoP (pOGG113), celB (pOGG050) and T-pharma (pOGG003)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Domesticated for Golden Gate cloning
    Promoter
    Available since
    April 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    133236-plasmid-map-sequence-id-287551

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  30. pOPS0383

    Plasmid
    #133233
    Purpose
    Reporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.
    Depositor
    Philip Poole
    Article
    Mendoza-Suarez et al Proc Natl Acad Sci U S A. 2020 May 5;117
    Insert
    constructed with NoP (pOGG113), sfGFP (pOGG037) and T-pharma (pOGG003)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Domesticated for Golden Gate cloning
    Promoter
    Available since
    April 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    133233-plasmid-map-sequence-id-287536

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  31. pOPS0380

    Plasmid
    #133230
    Purpose
    Reporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.
    Depositor
    Philip Poole
    Article
    Mendoza-Suarez et al Proc Natl Acad Sci U S A. 2020 May 5;117
    Insert
    constructed with constitutive promoter Rlv3841pnifH (pOGG082), mCherry (EC15071) and T-pharma (pOGG003)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Domesticated for Golden Gate cloning
    Promoter
    Available since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    133230-plasmid-map-sequence-id-278559

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  33. pSYN-VWF-039

    Plasmid
    #120292
    Purpose
    VWF expression construct expressing D'D3 domains with C1099A/C1142A mutations in D3 domain as an Fc fusion protein
    Depositor
    Robert Peters
    Article
    Dong et al Blood. 2019 Jan 14. pii: blood-2018-10-8
    Insert
    VWF D1-D2-D'-D3-Fc
    Use
    Tags
    Fc
    Expression
    Mammalian
    Mutation
    C1099A/C1142A
    Promoter
    CMV
    Available since
    Jan. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    120292-plasmid-map-sequence-id-233304

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  34. pMSCV-KD-mAbl

    Plasmid
    #69028
    Purpose
    Retorviral vector encoding Kinase Defective mouse c-Abl
    Depositor
    Jean Wang
    Article
    Sridevi et al PLoS One. 2013 Oct 11;8(10):e77495. doi:
    Insert
    mouse c-Abl Type VI - kinase defective
    Use
    Retroviral
    Tags
    FLAG tag inserted at Internal SalI site (2934 bp)
    Expression
    Mammalian
    Mutation
    The KD (kinase defective) mutation is at Lysine 2…
    Promoter
    Available since
    Sept. 2, 2015
    Availability
    Academic Institutions and Nonprofits only

  43. pX330_Tuba1b sgRNA / hSpCas9

    Plasmid
    #172835
    Purpose
    Mammalian expression of a sgRNA targeting the intron 1 of Tuba1b (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.
    Depositor
    Haining Zhong
    Article
    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1
    Insert
    sgRNA targeting intron 1 of Tuba1b under the U6 promotor and hSpCas9 under the CAG promotor
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    172835-plasmid-map-sequence-id-341082

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Showing: 6601 - 6650 of 8360 results