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Showing: 6651 - 6700 of 12238 results
  1. pEGB_3alpha2 OplacI:mini35S:RDF:Tnos (GB1534)

    Plasmid
    #160617
    Purpose
    TU for the regulated expression of the PhiC31 phage recombination directionality factor (RDF) gene driven by a synthetic promoter consisting of the LacI operator fused to the minimal 35S promoter.
    Depositor
    Insert
    RDF
    Use
    Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    mini35s
    Available since
    March 25, 2021
    Availability
    Academic Institutions and Nonprofits only
  2. pUPD2 PhiC31 RL (attR:P35S:TMtb:attL) (GB1506)

    Plasmid
    #160582
    Purpose
    35S promoter inverted sequence and the Mtb terminator flanked by two opposing PhiC31 site-specific recombination sites (attR and attL)
    Depositor
    Insert
    attR:P35S:TMtb:attL
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Available since
    March 25, 2021
    Availability
    Academic Institutions and Nonprofits only
  3. pEGB3 alpha2 OplexA:mini35S:PhiC31int:Tnos (GB1529)

    Plasmid
    #160614
    Purpose
    TU for the regulated expression of the PhiC31 phage integrase gene driven by a synthetic promoter consisting of the LexA operator fused to the minimal 35S promoter.
    Depositor
    Insert
    PhiC31
    Use
    Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    mini35s
    Available since
    Jan. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
  4. pEGB_3alpha1 OplacI:mini35S:phiC31:Tnos (GB1530)

    Plasmid
    #160615
    Purpose
    TU for the regulated expression of the PhiC31 phage integrase gene driven by a synthetic promoter consisting of the LacI operator fused to the minimal 35S promoter.
    Depositor
    Insert
    PhiC31
    Use
    Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    mini35s
    Available since
    Jan. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
  5. 1010-AIDx-CP

    Plasmid
    #135340
    Purpose
    Expression of circular permutant of nSpyCas9 at amino acid position 1010 encoding truncated human cytosine deaminase, hAIDx, at the C-terminus.
    Depositor
    Insert
    CP 1010 nSpyCas9 -hAIDx
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    WT
    Promoter
    Available since
    Oct. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
  6. 1058-AIDx-CP

    Plasmid
    #135342
    Purpose
    Expression of circular permutant of nSpyCas9 at amino acid position 1058 encoding truncated human cytosine deaminase, hAIDx, at the C-terminus.
    Depositor
    Insert
    CP 1058 nSpyCas9-hAIDx
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    WT
    Promoter
    Available since
    Oct. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
  7. pC0.007

    Plasmid
    #119671
    Purpose
    Level 0 part. Promoter, cpc560 promoter, extended spacer region, downstream TFB site region duplicated.
    Depositor
    Insert
    Pcpc560-Dx2
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    bp 89 T to C, bp 187 C to A, Duplicated downstrea…
    Promoter
    Available since
    Aug. 5, 2019
    Availability
    Academic Institutions and Nonprofits only
  8. pC0.006

    Plasmid
    #119670
    Purpose
    Level 0 part. Promoter, cpc560 promoter, extended spacer region, upstream TFB site region duplicated.
    Depositor
    Insert
    Pcpc560-Ux2
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    bp 89 T to C, bp 187 C to A, Duplicated upstream …
    Promoter
    Available since
    Aug. 5, 2019
    Availability
    Academic Institutions and Nonprofits only
  9. IF405: pMAGIC (R4-R3) NLS-Sa dCas9-NLS-p300core

    Plasmid
    #121825
    Purpose
    pMAGIC R4-R3 entry plasmid, contains 2x NLS Sa-dCas9 fused to the p300 catalytic core for 3 or 4-component MultiSite Gateway Pro assembly.
    Insert
    Sa-dCas9/p300core (open)
    Use
    Synthetic Biology; Pmagic gateway entry plasmid
    Tags
    NLS
    Expression
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
  10. KL001: pMAGIC (R4-R3) NLS-(SrfI/PmeI)-NLS-VPR

    Plasmid
    #121836
    Purpose
    pMAGIC R4-R3 entry plasmid, contains 2x NLS/VPR scaffold enabling addition of dCas9 mutants into pMAGIC. dCas9 can be inserted via unique SrfI/PmeI restriction sites
    Type
    Empty backbone
    Use
    Synthetic Biology; Pmagic gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
  11. KL101: pMAGIC (R4-R3) NLS-(SrfI/PmeI)-NLS-KRAB

    Plasmid
    #121837
    Purpose
    pMAGIC R4-R3 entry plasmid, contains 2x NLS/KRAB scaffold enabling addition of dCas9 mutants into pMAGIC. dCas9 can be inserted via unique SrfI/PmeI restriction sites
    Type
    Empty backbone
    Use
    Synthetic Biology; Pmagic gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
  12. KL202: pMAGIC (R4-R3) NLS-(SrfI/PmeI)-NLS-LSD1

    Plasmid
    #121838
    Purpose
    pMAGIC R4-R3 entry plasmid, contains 2x NLS/LSD1 scaffold enabling addition of dCas9 mutants into pMAGIC. dCas9 can be inserted via unique SrfI/PmeI restriction sites
    Type
    Empty backbone
    Use
    Synthetic Biology; Pmagic gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
  13. KA601: pMVP (L3-L2) pA; EF1a::TETa-pA

    Plasmid
    #121805
    Purpose
    pMVP L3-L2 entry plasmid, contains polyA + EF1a-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds EF1a-driven TETa downstream of gene of interest.
    Insert
    polyA + EF1a::TETa-polyA
    Use
    Synthetic Biology; Pmvp gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
  14. KA401: pMVP (L3-L2) pA; RIP::TETa-pA

    Plasmid
    #121809
    Purpose
    pMVP L3-L2 entry plasmid, contains polyA + RIP-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds RIP-driven TETa downstream of gene of interest.
    Insert
    polyA + RIP::TETa-polyA
    Use
    Synthetic Biology; Pmvp gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
  15. LF901: pMAGIC (L1-R5) mU6::SaCas9 gRNA scaffold

    Plasmid
    #121811
    Purpose
    pMAGIC L1-R5 entry plasmid, contains empty mouse U6-driven SaCas9 gRNA scaffold for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion.
    Type
    Empty backbone
    Use
    Synthetic Biology; Pmagic gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
  16. IA304: pMAGIC (R4-R3) NLS-Sa dCas9-NLS-VPR

    Plasmid
    #121822
    Purpose
    pMAGIC R4-R3 entry plasmid, contains 2x NLS Sa-dCas9 fused to the VPR transcriptional activator for 3 or 4-component MultiSite Gateway Pro assembly.
    Insert
    Sa-dCas9/VPR (open)
    Use
    Synthetic Biology; Pmagic gateway entry plasmid
    Tags
    NLS
    Expression
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
  17. IB801: pMAGIC (R4-R3) NLS-Sa dCas9-NLS-KRAB

    Plasmid
    #121823
    Purpose
    pMAGIC R4-R3 entry plasmid, contains 2x NLS Sa-dCas9 fused to the KRAB transcriptional repressor for 3 or 4-component MultiSite Gateway Pro assembly.
    Insert
    Sa-dCas9/KRAB (open)
    Use
    Synthetic Biology; Pmagic gateway entry plasmid
    Tags
    NLS
    Expression
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
  18. KZ301: pMVP (L3-L2) P2A-Blast + polyA

    Plasmid
    #121779
    Purpose
    pMVP L3-L2 entry plasmid, contains Blast-P2A + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term Blast selection marker linked by P2A to gene of interest.
    Insert
    P2A-Blast + polyA
    Use
    Synthetic Biology; Pmvp gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
  19. KZ201: pMVP (L3-L2) P2A-Hygro + polyA

    Plasmid
    #121782
    Purpose
    pMVP L3-L2 entry plasmid, contains Hygro-P2A + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term Hygro selection marker linked by P2A to gene of interest.
    Insert
    P2A-Hygro + polyA
    Use
    Synthetic Biology; Pmvp gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
  20. LA401: pMVP (L3-L2) P2A-Blast + WPRE

    Plasmid
    #121784
    Purpose
    pMVP L3-L2 entry plasmid, contains Blast-P2A + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term Blast selection marker linked by P2A to gene in lentivirus vectors
    Insert
    P2A-Blast + WPRE
    Use
    Synthetic Biology; Pmvp gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
  21. LA501: pMVP (L3-L2) P2A-Neo + WPRE

    Plasmid
    #121786
    Purpose
    pMVP L3-L2 entry plasmid, contains Neo-P2A + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term Neo selection marker linked by P2A to a gene in lentivirus vectors
    Insert
    P2A-Neo + WPRE
    Use
    Synthetic Biology; Pmvp gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
  22. LA701: pMVP (L3-L2) P2A-Zeo + WPRE

    Plasmid
    #121788
    Purpose
    pMVP L3-L2 entry plasmid, contains Zeo-P2A + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term Zeo selection marker linked by P2A to a gene in lentivirus vectors.
    Insert
    P2A-Zeo + WPRE
    Use
    Synthetic Biology; Pmvp gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
  23. KN401: pMVP (L3-L2) FKBP DD + WPRE

    Plasmid
    #121792
    Purpose
    pMVP L3-L2 entry plasmid, contains FKBP DD + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows fusion of C-term FKBP degron domain (stabilized by SHLD1) to gene in lentivirus vectors.
    Insert
    FKBP Degron Domain + WPRE
    Use
    Synthetic Biology; Pmvp gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
  24. KN501: pMVP (L3-L2) ecDHFR DD + WPRE

    Plasmid
    #121793
    Purpose
    pMVP L3-L2 entry plasmid, contains ecDHFR DD + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows fusion of C-term ecDHFR degron domain (stablized by TMP) to gene in lentivirus vectors
    Insert
    ecDHFR Degron Domain + WPRE
    Use
    Synthetic Biology; Pmvp gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
  25. IX702: pMVP (L3-L2) OLLAS-IRES-eGFP + polyA

    Plasmid
    #121748
    Purpose
    pMVP L3-L2 entry plasmid, contains OLLAS epitope tag + IRES2-eGFP + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion and IRES expression of eGFP reporter
    Insert
    OLLAS epitope tag-IRES2-eGFP + polyA
    Use
    Synthetic Biology; Pmvp gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
  26. KG901: pMVP (L3-L2) HA epitope tag + polyA

    Plasmid
    #121749
    Purpose
    pMVP L3-L2 entry plasmid, contains HA epitope tag + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest.
    Insert
    HA epitope tag + polyA
    Use
    Synthetic Biology; Pmvp gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
  27. IX503: pMVP (L3-L2) OLLAS epitope tag + polyA

    Plasmid
    #121752
    Purpose
    pMVP L3-L2 entry plasmid, contains OLLAS epitope tag + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest.
    Insert
    OLLAS epitope tag + polyA
    Use
    Synthetic Biology; Pmvp gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
  28. IX401: pMVP (L3-L2) 6x His epitope tag + polyA

    Plasmid
    #121754
    Purpose
    pMVP L3-L2 entry plasmid, contains 6x His epitope tag + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest.
    Insert
    6x His epitope tag + polyA
    Use
    Synthetic Biology; Pmvp gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
  29. KH101: pMVP (L3-L2) HA epitope tag + WPRE

    Plasmid
    #121756
    Purpose
    pMVP L3-L2 entry plasmid, contains HA epitope tag + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest in lentivirus vectors.
    Insert
    HA epitope tag + WPRE
    Use
    Synthetic Biology; Pmvp gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
  30. IX907: pMVP (L3-L2) 3x epitope tag + WPRE

    Plasmid
    #121757
    Purpose
    pMVP L3-L2 entry plasmid, contains 3x HA epitope tag + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest in lentivirus vectors.
    Insert
    3x HA epitope tag + WPRE
    Use
    Synthetic Biology; Pmvp gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
  31. JA001: pMVP (L3-L2) FLAG epitope tag + WPRE

    Plasmid
    #121758
    Purpose
    pMVP L3-L2 entry plasmid, contains FLAG epitope tag + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest in lentivirus vectors.
    Insert
    FLAG epitope tag + WPRE
    Use
    Synthetic Biology; Pmvp gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
  32. IZ901: pMVP (L3-L2) OLLAS epitope tag + WPRE

    Plasmid
    #121759
    Purpose
    pMVP L3-L2 entry plasmid, contains OLLAS epitope tag + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest in lentivirus vectors.
    Insert
    OLLAS epitope tag + WPRE
    Use
    Synthetic Biology; Pmvp gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
  33. IZ702: pMVP (L3-L2) myc epitope tag + WPRE

    Plasmid
    #121760
    Purpose
    pMVP L3-L2 entry plasmid, contains myc epitope tag + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest in lentivirus vectors.
    Insert
    myc epitope tag + WPRE
    Use
    Synthetic Biology; Pmvp gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
  34. IZ801: pMVP (L3-L2) 6x His epitope tag + WPRE

    Plasmid
    #121761
    Purpose
    pMVP L3-L2 entry plasmid, contains 6x His epitope tag + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest in lentivirus vectors.
    Insert
    6x His epitope tag + WPRE
    Use
    Synthetic Biology; Pmvp gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
  35. IH201: pMVP (L5-L4) 3x HA epitope tag

    Plasmid
    #121700
    Purpose
    pMVP L5-L4 entry plasmid, contains 3x HA epitope tag for 4-component MultiSite Gateway Pro assembly. Allows N-term epitope tag fusion to gene of interest.
    Insert
    3x HA epitope tag (open)
    Use
    Synthetic Biology; Pmvp gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
  36. KG703: pMVP (L5-L4) 6x His epitope tag

    Plasmid
    #121706
    Purpose
    pMVP L5-L4 entry plasmid, contains 6x His epitope tag for 4-component MultiSite Gateway Pro assembly. Allows N-term epitope tag fusion to gene of interest.
    Insert
    6x His epitope tag (open)
    Use
    Synthetic Biology; Pmvp gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
Showing: 6651 - 6700 of 12238 results