We narrowed to 15,045 results for: NOL

1. pLenti_CMV:Flag-OeNL

   Plasmid

   #208850

   Purpose

   Expresses FLAG epitope-tagged OeNL "Orange" BRET reporter in mammalian cells.

   Depositor

   Antonio Amelio

   Article

   Labra et al iScience. 2024 Aug 8;27(9):110655. doi:

   Insert

   OeNL Nanolantern
   

   Use

   Lentiviral

   Tags

   1xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pGWB409

   Plasmid

   #74803

   Purpose

   Gateway cloning compatible binary vector for N-terminal fusion with 6xHis (CaMV35S promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   CaMV35S

   Available since

   Nov. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pPE3-pPuro

   Plasmid

   #215722

   Purpose

   Cloning vector for Uni-Vector prime editing system

   Depositor

   Kou-Juey Wu

   Article

   Huang et al Biotechnol J. 2024 Feb;19(2):e2300353. d

   Insert

   PE2
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   EFS promoter

   Available since

   Dec. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pMXs-ms-Sox7

   Plasmid

   #50780

   Purpose

   retroviral expression of mouse Sox7

   Depositor

   Shinya Yamanaka

   Article

   Nakagawa et al Nat Biotechnol. 2008 Jan;26(1):101-6. Ep

   Insert

   Sox7 (Sox7 Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 28, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCast

   Plasmid

   #200169

   Purpose

   E. coli/Yeast shuttle vector carrying CloNAT marker and Spcas9 allowing cloning of gRNA flanked by tRNA promoter and HDV ribozyme

   Depositor

   Yong-Su Jin

   Article

   Kuanyshev et al Biotechnol Bioeng. 2021 Jan;118(1):372-3

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pEGFP-CLCa

   Plasmid

   #196912

   Purpose

   Visualization of clathrin-coated pit formation in living mammalian cells

   Depositor

   Kenji Sugimoto

   Article

   Sakaushi et al Biosci Biotechnol Biochem. 2007 Feb;71(2

   Insert

   Clathrin-Coated light chain
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAF0443 Nm60_attP-mCherry donor

   Plasmid

   #193471

   Purpose

   Promoterless mcherry donor for Nm60

   Depositor

   Patrick Hsu

   Article

   Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

   Insert

   Nm60_attP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCaRNA1&2

   Plasmid

   #86087

   Purpose

   Simultaneous expression of cDNAs of complete genomic RNA1 and RNA 2 of PNRSV (Pch12 isolate) in transfected cells

   Depositor

   Aiming Wang

   Article

   Cui et al Plant Biotechnol J. 2016 Aug 27. doi: 10

   Insert

   RNA1, RNA2
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   35S

   Available since

   Feb. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCaRNA3

   Plasmid

   #86088

   Purpose

   Expression of cDNA of full-length genomic RNA3 of PNRSV (Pch12 isolate) in transfected cells

   Depositor

   Aiming Wang

   Article

   Cui et al Plant Biotechnol J. 2016 Aug 27. doi: 10

   Insert

   RNA3
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   35S

   Available since

   Jan. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGWB509

    Plasmid

    #74851

    Purpose

    Gateway cloning compatible binary vector for N-terminal fusion with 6xHis (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV35S

    Available since

    Sept. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pGWB543

    Plasmid

    #74877

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with eCFP (no promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Oct. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pKB33 (pUC19-KANMX-tetO7-CYCpr)

    Plasmid

    #210472

    Purpose

    Plasmid with KanMX-tetO7-CYCpr cassette from the yTHC collection. Used for amplifying cassette with homology to regions of interest for genomic integration.

    Depositor

    Christian Landry

    Article

    Evans-Yamamoto et al Mol Biol Evol. 2023 Nov 18:msad246. doi:

    Insert

    KanMX-TetO7-CYCpr cassette
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3.2/EV

    Plasmid

    #120833

    Purpose

    Control vector, 20nt random sequence between gateway recombination sites

    Depositor

    Jan Rehwinkel

    Article

    Hertzog et al Eur J Immunol. 2018 Mar 23. doi: 10.1002

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3.2/pr-Membrane

    Plasmid

    #120817

    Purpose

    Ectopic expression of ZIKV protein

    Depositor

    Jan Rehwinkel

    Article

    Hertzog et al Eur J Immunol. 2018 Mar 23. doi: 10.1002

    Insert

    pr-Membrane ZIKV
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAF0466 Ef1a-Nm60_attB acceptor

    Plasmid

    #193476

    Purpose

    Acceptor plasmid for 3 plasmid recombination assay

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    Nm60_attB
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. SaCas9 TadDE

    Plasmid

    #193846

    Purpose

    Express TadDE (with SaCas9) in mammalian cells

    Depositor

    David Liu

    Article

    Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

    Insert

    TadDE with SaCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. p4A8_S7T_BC

    Plasmid

    #217832

    Purpose

    4A8 variant in barcoded, yeast surface display plasmid

    Depositor

    Timothy Whitehead

    Article

    Petersen et al Nat Commun. 2024 May 10;15(1):3974. doi:

    Insert

    4A8 variant Fab
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    VL: S7T

    Promoter

    Available since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGWB412

    Plasmid

    #74806

    Purpose

    Gateway cloning compatible binary vector for N-terminal fusion with FLAG (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV35S

    Available since

    Oct. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. ND4.2-Left TALE-G1397-N-DddA6

    Plasmid

    #179680

    Purpose

    Expression of base editor in mammalian cells

    Depositor

    David Liu

    Article

    Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

    Insert

    COX8a MTS_3xFLAG_ND4.2 Left TALE_2aa_DddA G1397-N (Q1310R S1330I T1380I)_1xUGI_ATP5B 3'UTR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Q1310R, S1330I, T1380I

    Promoter

    Available since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pT2-CAG-fGFP

    Plasmid

    #108714

    Purpose

    Transposon donor plasmid for long-term expression of membrane targetted GFP

    Depositor

    Madeline Lancaster

    Article

    Lancaster et al Nat Biotechnol. 2017 Jul;35(7):659-666.

    Insert

    EGFP
    

    Use

    Tags

    6xHis and farnesylation signal

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pv6_EGFP

    Plasmid

    #179392

    Purpose

    Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of EGFP

    Depositor

    Tuncay Baubec

    Article

    Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

    Insert

    EGFP
    

    Use

    Tags

    AviTag

    Expression

    Mammalian

    Mutation

    Promoter

    CAGGS

    Available since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pcDNA3.2/NS5

    Plasmid

    #120825

    Purpose

    Ectopic expression of ZIKV protein

    Depositor

    Jan Rehwinkel

    Article

    Hertzog et al Eur J Immunol. 2018 Mar 23. doi: 10.1002

    Insert

    NS5 ZIKV
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAAV-GfaABC1D-GRAB_eCB2.0

    Plasmid

    #164609

    Purpose

    Express the endocannabinoid sensor GRAB_eCB2.0 in astrocytes

    Depositor

    Yulong Li

    Article

    Dong et al Nat Biotechnol. 2021 Nov 11. pii: 10.103

    Insert

    Endocannabinoid sensor GRAB_eCB2.0
    

    Use

    AAV

    Tags

    Expression

    Mutation

    S383T

    Promoter

    GfaABC1D

    Available since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pGL3-Promoter-4xBS_BHLHE40

    Plasmid

    #153064

    Purpose

    Four BHLHE40 consensus binding sites cloned upstream of luciferase reporter gene

    Depositor

    Silvia Monticelli

    Article

    Emming et al Nat Immunol. 2020 Apr;21(4):388-399. doi

    Insert

    4X consensus BHLHE40 binding sites
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    July 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA3.2/NS2A

    Plasmid

    #120818

    Purpose

    Ectopic expression of ZIKV protein

    Depositor

    Jan Rehwinkel

    Article

    Hertzog et al Eur J Immunol. 2018 Mar 23. doi: 10.1002

    Insert

    NS2A ZIKV
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pTRE3G-iRFP-B1-timestamp_A

    Plasmid

    #159924

    Purpose

    Timestamp Template A inserted into the 3’ UTR of iRFP between bActin Zipcode element and WPRE, with RNA barcode TGC. Also includes a xrRNA element in the 5’ UTR. AKA 147B1.

    Depositor

    Fei Chen

    Article

    Rodriques et al Nat Biotechnol. 2020 Oct 19. pii: 10.103

    Insert

    iRFP-Timestamp A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    Dec. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pMK365 (Tet-OsTIR1-loxP-PURO-loxP)

    Plasmid

    #121185

    Purpose

    OsTIR1 introduction

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

    Insert

    Tet-OsTIR1-loxP-PURO-loxP
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    OsTIR1 codon optimized for human cells

    Promoter

    Available since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pGWB408

    Plasmid

    #74802

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with 6xHis (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV35S

    Available since

    Aug. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. AA302

    Plasmid

    #216052

    Purpose

    Fragmid fragment: (C' terminus) Nucleoplasmin NLS

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    NpNLS_v1.2; Stop; polyA_v1
    

    Use

    Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pFEndoNA

    Plasmid

    #174724

    Purpose

    Expresses GFP-endoNA fusion protein for degradation of polysialic acid. The endoNA is a catalytically active endosialidase from bacteriophage PK1A.

    Depositor

    Jukka Finne

    Article

    Jokilammi et al J Immunol Methods. 2004 Dec;295(1-2):149

    Insert

    GFP-endoNA fusion protein
    

    Use

    Tags

    Histag

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pL-SFFV.Reporter.RFP657.PAC

    Plasmid

    #61395

    Purpose

    Lentiviral vector for RFP657 NHEJ reporter system expression. Allows insertion of target sites between the SFFV promoter and RFP657 for detection of fluorescence knockdown. Puromycin resistance.

    Depositor

    Benjamin Ebert

    Article

    Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    May 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pLVX-Tight-Puro_Olig2

    Plasmid

    #64842

    Purpose

    A lentiviral vector for the expression of mouse Olig2 under doxycyline control

    Depositor

    Paul Tesar

    Article

    Najm et al Nat Biotechnol. 2013 May;31(5):426-33. d

    Insert

    Olig2 (Olig2 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    May 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pTpal-fdc

    Plasmid

    #78288

    Purpose

    Expresses A. thaliana PAL2 and S. cerevisiae FDC1 in E. coli

    Depositor

    David Nielsen

    Article

    McKenna et al Biotechnol J. 2013 Dec;8(12):1465-75. do

    Inserts

    PAL2 (PAL2 Mustard Weed)
    FDC1 (FDC1 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    *see below

    Promoter

    trc

    Available since

    June 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pU6-Sp-gRNA-ALB_DF_B358c_attB

    Plasmid

    #182146

    Purpose

    To insert attB attachment site at ALB in human cells via twinPE

    Depositor

    David Liu

    Article

    Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

    Insert

    ALB_B358c_attB pegRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    March 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pU6-Sp-gRNA-ALB_DF_A277c_attB

    Plasmid

    #182145

    Purpose

    To insert attB attachment site at ALB in human cells via twinPE

    Depositor

    David Liu

    Article

    Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

    Insert

    Alb_A277c_attB pegRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    March 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pET16b-Trx CC32/35SS

    Plasmid

    #36946

    Depositor

    Anna Blom

    Article

    King et al J Immunol. 2012 Apr 15;188(8):4103-12. E

    Insert

    Thioredoxin 1 (TXN Human)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    both active site cysteines (32,35) mutated to ser…

    Promoter

    T7

    Available since

    May 20, 2013

    Availability

    Academic Institutions and Nonprofits only

37. pcDNA3.2/NS4A

    Plasmid

    #120826

    Purpose

    Ectopic expression of ZIKV protein

    Depositor

    Jan Rehwinkel

    Article

    Hertzog et al Eur J Immunol. 2018 Mar 23. doi: 10.1002

    Insert

    NS4A ZIKV
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pICSL12009

    Plasmid

    #68257

    Purpose

    Level 0 Golden Gate Part: Promoter and 5UTR Ubiquitin (Zea mays)

    Depositor

    Nicola Patron

    Article

    Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

    Insert

    Promoter and 5UTR Ubiquitin (Zea mays)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pSL2669 (pTnsABf_Vch)

    Plasmid

    #200897

    Purpose

    Expresses human codon-optimized V. cholerae TnsA-TnsB fusion from a CMV promoter.

    Depositor

    Samuel H. Sternberg

    Article

    Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

    Insert

    VchTnsA-TnsB
    

    Use

    Tags

    Internal NLS between TnsA-TnsB fusion

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pAsCpf1(TYCV)(BB) (pY211)

    Plasmid

    #89352

    Purpose

    Expresses humanized AsCpf1 TYCV PAM variant and crRNA guide

    Depositor

    Feng Zhang

    Article

    Gao et al Nat Biotechnol. 2017 Jun 5. doi: 10.1038

    Insert

    Cpf1, RR variant
    

    Use

    CRISPR

    Tags

    NLS and NLS-3xHA

    Expression

    Mammalian

    Mutation

    S542R/K607R

    Promoter

    CBh

    Available since

    July 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pLenti-CaMKIIa-Archer1-EGFP

    Plasmid

    #60423

    Purpose

    Lentiviral vector with CaMKIIa promoter and upstream CMV promoter expressing Archer1-EGFP fusion. Archer1's red fluorescence monitors changes in membrane voltage.

    Depositor

    Viviana Gradinaru

    Article

    Flytzanis et al Nat Commun. 2014 Sep 15;5:4894. doi: 10.

    Insert

    Archer1-EGFP
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CaMKIIa

    Available since

    Nov. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pBGSA FPR1-EGFP

    Plasmid

    #62604

    Purpose

    Constitutive expression of FPR1-EGFP in mammalian cells

    Depositor

    Heini Miettinen

    Article

    Gripentrog et al J Immunol. 2003 Sep 15;171(6):3187-93.

    Insert

    FPR1 (FPR1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    SRalpha

    Available since

    June 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pBK-EQR-BE3

    Plasmid

    #85172

    Purpose

    Expresses EQR-BE3 in mammalian cells

    Depositor

    David Liu

    Article

    Kim et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

    Insert

    EQR-BE3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. LbABE8e(TadA-8e V106W)

    Plasmid

    #138508

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

    Insert

    ecTadA(8e V106W)-dLbCas12a
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    TadA-8e V106W

    Promoter

    Available since

    April 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. AA011

    Plasmid

    #215973

    Purpose

    Fragmid fragment: (N' terminus) for C>T base editing

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    Start; BPNLS_v1.1; rAPOBEC1_v1.1; Linker_v1.1
    

    Use

    Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. AA010

    Plasmid

    #216038

    Purpose

    Fragmid fragment: (C' terminus) for C>T base editing

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    UGI_v1.1; NpNLS_v1.1
    

    Use

    Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. AA013

    Plasmid

    #215975

    Purpose

    Fragmid fragment: (N' terminus) for A>G base editing

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    Start; BPNLS_v1.1; ABE8e_v1.1; Linker_v1.1
    

    Use

    Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pCellFree_G03 GDI2

    Plasmid

    #67093

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    GDI2 (GDI2 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

49. pcDNA3.2/NS4B

    Plasmid

    #120823

    Purpose

    Ectopic expression of ZIKV protein

    Depositor

    Jan Rehwinkel

    Article

    Hertzog et al Eur J Immunol. 2018 Mar 23. doi: 10.1002

    Insert

    NS4B ZIKV
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pRN110

    Plasmid

    #127228

    Purpose

    CmYLCV::STM::35S terminator

    Depositor

    Daniel Voytas

    Article

    Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

    Insert

    STM (STM A. tumefaciens, Mustard Weed)
    

    Use

    Tags

    Expression

    Plant

    Mutation

    codon optimized CDSs

    Promoter

    Available since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge