162,325 results

1. pBECAb-apr

   Plasmid

   #122001

   Purpose

   A cytidine deaminase-mediated base-editing plasmid in Acinetobacter baumannii

   Depositor

   Quanjiang Ji

   Article

   Wang et al Cell Chem Biol. 2019 Sep 17. pii: S2451-

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. RED

   Plasmid

   #139835

   Purpose

   Expresses RNase H1-EGFP- dCas9 fusion protein in human cells

   Depositor

   Karim Mekhail

   Article

   Abraham et al Nature. 2020 Sep;585(7824):298-302. doi:

   Insert

   RNase H1-EGFP-dCas9 (RNASEH1 RNase H1 (H. sapiens), dCas9 (S. pyogenes))
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   dCas9 protein contains two mutations: D10A and H8…

   Promoter

   tet-inducible CMV

   Available since

   Sept. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAAV_BiPVe4_eGFP

   Plasmid

   #221463

   Purpose

   AAV construct expressing eGFP driven by chandelier cell-targeting enhancer. Alias: pAAV_WDC0004_eGFP

   Depositor

   Gordon Fishell

   Article

   Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

   Insert

   eGFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pNCS-mClover3

   Plasmid

   #74236

   Purpose

   Constitutive expression of mClover3 in bacteria

   Depositor

   Michael Lin

   Article

   Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.10

   Has Service

   Cloning Grade DNA

   Insert

   mClover3
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAAV_BiVIPe4_GCaMP6f

   Plasmid

   #213859

   Purpose

   AAV construct expressing GCaMP6f driven by VIP interneuron-targeting enhancer. Alias: pAAV_YWE4.2_GCaMP6f

   Depositor

   Gordon Fishell

   Article

   Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

   Insert

   GCaMP6f
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pHLSec2-FN-YPet

   Plasmid

   #65421

   Purpose

   Expresses in mammalian cells human plasma fibronectin with YPet inserted between FNIII domains 6 and 7

   Depositor

   Harold Erickson

   Article

   Ohashi et al J Biol Chem. 2011 Nov 11;286(45):39188-9

   Insert

   human plasma fibronectin with YPet inserted between FNIII domains 6 and 7 (FN1 Human)
   

   Use

   Tags

   YPet

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Epac-S-H74

   Plasmid

   #170338

   Purpose

   mTDel_EPACdDEPCD_cp173Ven(ST)_Ven(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.

   Depositor

   Kees Jalink

   Article

   Klarenbeek et al PLoS One. 2011 Apr 29;6(4):e19170. doi:

   Insert

   mTDel_EPACdDEPCD_cp173Ven(ST)_Ven(ST) (RAPGEF3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Deletion of DEP domain, Catalytically dead T781A …

   Promoter

   CMV

   Available since

   June 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. CD40L-SrtA

   Plasmid

   #121167

   Purpose

   pMP71 vector expressing Tomato - P2A - CD40L - SrtA - flag

   Depositor

   Gabriel Victora

   Article

   Pasqual et al Nature. 2018 Jan 25;553(7689):496-500. d

   Insert

   CD40L-SrtA
   

   Use

   Retroviral

   Tags

   Tomato and flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Rosa26 mT/mG

   Plasmid

   #17787

   Depositor

   Liqun Luo

   Article

   Muzumdar et al Genesis. 2007 Sep . 45(9):593-605.

   Insert

   Rosa26 mT/mG
   

   Use

   Cre/Lox and Mouse Targeting

   Tags

   membrane tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 14, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3.3-NL63-D14

    Plasmid

    #172666

    Purpose

    encodes NL63 spike protein, for pseudovirus production

    Depositor

    David Nemazee

    Article

    Dacon et al Science. 2022 Jul 12:eabq3773. doi: 10.1

    Insert

    HCNV63gp2 (HCNV63gp2 human coronavirus NL63)
    

    Use

    Lentiviral and Retroviral

    Tags

    NA

    Expression

    Mammalian

    Mutation

    c-terminal deletion of 14aa

    Promoter

    CMV

    Available since

    Jan. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV/D374Y-hPCSK9

    Plasmid

    #58379

    Purpose

    Expresses GoF mutant human PCSK9 to be used for rAAV8-mediated gene transfer, hypercholesterolemia and atherosclerosis

    Depositor

    Jacob Bentzon

    Article

    Bjorklund et al Circ Res. 2014 May 23;114(11):1684-9. do

    Insert

    proprotein convertase subtilisin/kexin type 9 (PCSK9 Human)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    D374Y

    Promoter

    HCRApoE/hAAT

    Available since

    Aug. 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. f-CTS-EGFP

    Plasmid

    #86064

    Purpose

    A mammalian expression plasmid encoding fibrocystin ciliary targeting signal domain with C-terminus EGFP.

    Depositor

    Lei Lu

    Article

    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

    Insert

    Fibrocystin (Pkhd1 Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    contains ciliary targeting signal (3870-3887 amin…

    Promoter

    CMV

    Available since

    May 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pOPINK-OTUD2 (full-length, aa 1-348)

    Plasmid

    #61409

    Purpose

    Expresses human OTUD2 (full-length) in E. coli.

    Depositor

    David Komander

    Article

    Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

    Insert

    OTUD2 (YOD1 Human)
    

    Use

    Tags

    His6-GST-3C

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. MIG-Luciferase-IRES-GFP

    Plasmid

    #75021

    Purpose

    Encodes the expression of Luciferase and GFP

    Depositor

    Saradhi Mallampati

    Insert

    Luciferase
    

    Use

    Luciferase and Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. SlugCas9

    Plasmid

    #163793

    Purpose

    Expresses SlugCas9, and cloning backbone for sgRNA

    Depositor

    Yongming Wang

    Article

    Hu et al Nucleic Acids Res. 2021 Mar 15. pii: 617

    Type

    Empty backbone

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. NG-ABEmax

    Plasmid

    #124163

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

    Insert

    TadA-TadA(evo)-Cas9-NG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLenti-Tet

    Plasmid

    #138086

    Purpose

    Empty vector with Tet-response promoter for inducible expression of proteins. Can be transduced using Lentiviral particles

    Depositor

    Eva Frickel

    Article

    Fisch et al EMBO J. 2019 Jul 1;38(13):e100926. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    minimal CMV promoter with heptamerized tet‐operat…

    Available since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. AAVS1-Puro CAG FUCCI

    Plasmid

    #136934

    Purpose

    donor plasmid for constitutive FUCCI expression in human cells

    Depositor

    Xiaoping Bao

    Article

    Chang et al Biotechnol Bioeng. 2020 Apr 11. doi: 10.

    Insert

    Clover-Geminin(1-110)-IRES-mKO2-Cdt(30-120)
    

    Use

    Synthetic Biology

    Tags

    Clover and mKO2

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA4 myc PGC-1 alpha

    Plasmid

    #10974

    Purpose

    Mammalian expression of PGC-1a with myc and his tags

    Depositor

    Toren Finkel

    Article

    Ichida et al J Biol Chem. 2002 Dec 27. 277(52):50991-

    Insert

    PGC-1 alpha (PPARGC1A Human)
    

    Use

    Tags

    His and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. TFORF1037

    Plasmid

    #144269

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    WT1 (WT1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. HA-Sos1

    Plasmid

    #32920

    Depositor

    Dafna Bar-Sagi

    Article

    Yang et al J Biol Chem. 1995 Aug 4;270(31):18212-5.

    Insert

    SOS1 (SOS1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 31, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pcDNA3.1 mycBioID

    Plasmid

    #35700

    Purpose

    To fuse your protein of interest to the C-terminus of BirA(R118G) and use in proximity-dependent biotin identification (BioID); myc tag

    Depositor

    Kyle Roux

    Article

    Roux et al J Cell Biol. 2012 Mar 12.

    Has Service

    Cloning Grade DNA

    Insert

    BirA
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    R118G (highly promiscuous form)

    Promoter

    cmv

    Available since

    April 5, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. GB1-A11(2-196, G38R)-Strep

    Plasmid

    #196209

    Purpose

    Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(2-196, G38R)-Strep.

    Depositor

    Lalit Deshmukh

    Article

    Shihora et al ACS Chem Neurosci. 2023 Jul 11. doi: 10.

    Insert

    Annexin A11 (ANXA11 Human)
    

    Use

    Tags

    GB1-6xHis-TEV and Strep-II tag

    Expression

    Bacterial

    Mutation

    G38R

    Promoter

    Available since

    July 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. MSCV-CMV-DsRed-IRES-d1EGFP

    Plasmid

    #41942

    Purpose

    Retroviral expression of DsRed in mammalian cells

    Depositor

    Stephen Elledge

    Article

    Yen et al Science. 2008 Nov 7;322(5903):918-23. do

    Insert

    DsRed
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    May 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. AiP5003 pAAV-Syn-DIO-TVA66T-dTom-CVS-N2cG

    Plasmid

    #176285

    Purpose

    Cre-dependent AAV virus

    Depositor

    Shenqin Yao, The Allen Institute for Brain Science

    Article

    Yao et al Nat Neurosci. 2023 Feb;26(2):350-364. do

    Insert

    TVA66T-dTomato-rabies Glycoprotein
    

    Use

    Tags

    dTomato

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. mCherry-Clathrin LC-15

    Plasmid

    #55019

    Purpose

    Localization: Clathrin Vesicles, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Article

    Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

    Insert

    Clathrin LC (CLTB Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAAV.TBG.PI.Null.bGH

    Plasmid

    #105536

    Purpose

    empty AAV vector with TBG promoter

    Depositor

    James M. Wilson

    Has Service

    AAV8

    Insert

    None
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TBG

    Available since

    Feb. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pInducer20-Blast

    Plasmid

    #109334

    Purpose

    pInducer20 with added blasticidin resistance

    Depositor

    Jean Cook

    Article

    Matson et al Elife. 2017 Nov 17;6. doi: 10.7554/eLife

    Type

    Empty backbone

    Use

    Lentiviral; Inducible expression

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAAV-pCMV-TM-KA2-CaM-NES-TevN-AsLOV2-TEVseq-tTA

    Plasmid

    #171617

    Purpose

    AAV Soma-targeted Cal-Light with KA2

    Depositor

    Hyungbae Kwon

    Article

    Hyun et al Nat Commun. 2022 Dec 12;13(1):7692. doi:

    Insert

    TM-KA2-CaM-NES-TevN-AsLOV2-TEVseq-tTA
    

    Use

    AAV and Synthetic Biology

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pMevT

    Plasmid

    #17815

    Depositor

    Jay Keasling

    Article

    Martin et al Nat Biotechnol. 2003 Jul . 21(7):796-802

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 16, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pBabe-HAZ

    Plasmid

    #17383

    Depositor

    Stephen Goff

    Article

    Gao et al Science. 2002 Sep 6. 297(5587):1703-6.

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. mApple-Farnesyl-5

    Plasmid

    #54899

    Purpose

    Localization: Plasma Membrane, Excitation: 568, Emission: 592

    Depositor

    Michael Davidson

    Insert

    Farnesyl (HRAS Human)
    

    Use

    Tags

    mApple

    Expression

    Mammalian

    Mutation

    encodes final 20 aa of NM_001130442.1

    Promoter

    CMV

    Available since

    Aug. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

33. pscALPS- SIV-MAC251 vpx

    Plasmid

    #115810

    Purpose

    Encodes codon optimized 3xFLAG-VpxMAC251 and puromycin resistance protein

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

    Insert

    SIVMAC251 Vpx
    

    Use

    Lentiviral

    Tags

    3x-FLAG

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pAAV-CaMKIIa-hChR2(H134R)-EYFP

    Plasmid

    #26969

    Purpose

    AAV expression of humanized ChR2(H134R) fused to EYFP driven by CaMKIIa promoter for optogenetic activation

    Depositor

    Karl Deisseroth

    Article

    Lee et al Nature. 2010 Jun 10. 465(7299):788-92.

    Has Service

    AAV1, AAV2, AAV5, and AAV9

    Insert

    hChR2(H134R)
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Histidine 134 is mutated to Arginine

    Promoter

    CaMKIIa

    Available since

    Feb. 4, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCMV-SpRYc-ABE8e-EGFP

    Plasmid

    #208340

    Purpose

    Expresses SpRYc-ABE8e in mammalian cells

    Depositor

    Pranam Chatterjee

    Article

    Zhao et al Nat Commun. 2023 Oct 4;14(1):6175. doi:

    Insert

    ecTadA(8e)-nSpRYc
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Sc++ (1-1119), SpRY (1111-1368), D10A

    Promoter

    CMV

    Available since

    Aug. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pUC18T-mini-Tn7T-Gm-dsRedExpress

    Plasmid

    #65032

    Purpose

    GmR mini-Tn7 vector, mobilizable, for RFP tagging bacteria

    Depositor

    Herbert Schweizer

    Article

    Choi et al Nat Protoc. 2006;1(1):153-61.

    Insert

    dsRedExpress
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 14, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

37. paGFP-Omp25

    Plasmid

    #69598

    Purpose

    expresses GFP-labeled mitochondrial outer membrane protein 25

    Depositor

    David Sabatini

    Article

    Katajisto et al Science. 2015 Apr 17;348(6232):340-3. do

    Insert

    paGFP-Omp25 (Synj2bp Rat)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    photoactivatable GFP fused to rat Omp25 C-terminus

    Promoter

    CMV

    Available since

    Dec. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pLVX-mCherry

    Plasmid

    #180646

    Purpose

    Lentiviral expression vector for mCherry. Dox-inducible. Internal ID: WISP20-61.

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. TRPM7 gRNA (BRDN0001147821)

    Plasmid

    #76112

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human TRPM7

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    TRPM7 (TRPM7 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCFD3-ovoD1

    Plasmid

    #111142

    Purpose

    Expresses U6:3-sgRNA targeting ovoD1 mutation in Drosophila melanogaster

    Depositor

    Norbert Perrimon

    Article

    Ewen-Campen et al G3 (Bethesda). 2018 Jul 31;8(8):2749-275

    Insert

    pCFD3-ovoD1 (ovo Fly)
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    U6:3

    Available since

    Jan. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pJFRC-13XLexAoP-FRT-STOP-FRT-myrGFP-2A-KDR::Pest

    Plasmid

    #217515

    Purpose

    Encodes a LexAoP controlled and Flp dependent conditional trangene of bicistronic myrGFP and KD Recombinase

    Depositor

    S. Lawrence Zipursky

    Article

    Sanfilippo et al Neuron. 2024 Jan 13:S0896-6273(23)00973-

    Insert

    13XLexAoP-FRT-STOP-FRT-myrGFP-2A-KDR::Pest
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. LAMP1-SBP-GFP

    Plasmid

    #120172

    Purpose

    Expresses a chimera of LAMP1, SBP tag and GFP (fluorescent tag)

    Depositor

    Juan Bonifacino

    Article

    Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

    Insert

    Lysosomal-associated membrane protein 1 (Lamp1 Rat)
    

    Use

    Tags

    SBP and eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pSBbi-Neo

    Plasmid

    #60525

    Purpose

    SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: neomycin resistance gene

    Depositor

    Eric Kowarz

    Article

    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

    Type

    Empty backbone

    Use

    Transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a/RPBSA

    Available since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pCI-VSVG

    Plasmid

    #1733

    Purpose

    VSV-G expressing envelope vector; recommended for the FELIX (FIV-based) vector system from the Nolan lab

    Depositor

    Garry Nolan

    Insert

    VSV-G Envelope
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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45. TFORF3081

    Plasmid

    #144557

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ELK3 (ELK3 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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46. pLenti-myc-GLUT4-mCherry

    Plasmid

    #64049

    Purpose

    3rd generation lentiviral transfer plasmid. Reporter for GLUT4 translocation

    Depositor

    Weiping Han

    Article

    Lim et al Nat Commun. 2015 Jan 9;6:5951. doi: 10.1

    Insert

    GLUT4 (Slc2a4 Mouse)
    

    Use

    Lentiviral

    Tags

    c-Myc and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    July 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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47. ovoD1 D1B2NR (18 kb)

    Plasmid

    #37280

    Depositor

    Norbert Perrimon

    Article

    Chou et al Development. 1993 Dec;119(4):1359-69.

    Insert

    ovoD1 genomic DNA fragment
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    July 30, 2012

    Availability

    Academic Institutions and Nonprofits only

48. wt HA-dynamin 1 pcDNA3.1

    Plasmid

    #34682

    Depositor

    Sandra Schmid

    Insert

    dynamin 1ba (DNM1L Human)
    

    Use

    Tags

    HA (variant)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 31, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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49. GST-Delta23C11orf83

    Plasmid

    #65849

    Purpose

    bacterial expression of GST (N-ter) - Delta 23 C11orf83/UQCC3 (UQCC3 protein depleted of its N-terminal transmembrane domain)

    Depositor

    Amos Bairoch

    Article

    Desmurs et al Mol Cell Biol. 2015 Apr;35(7):1139-56. d

    Insert

    UQCC3 depleted of its N terminal transmembrane part (UQCC3 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    deletion of the 23 first aa (TM)

    Promoter

    Available since

    July 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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50. pSIE1

    Plasmid

    #136355

    Purpose

    Selection by Inducible Effectors

    Depositor

    Andrew Goodman

    Article

    Bencivenga-Barry et al J Bacteriol. 2020 Jan 15;202(3). pii: JB

    Inserts

    Type VI effector bfe1
    Type VI effector bte1
    BT_4676
    tetR
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    ATG removed to add signal sequence before gene

    Promoter

    P1TDP-GHO23

    Available since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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