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We narrowed to 18,985 results for: INO

Showing: 7251 - 7300 of 18985 results

  6. pTEE

    Plasmid
    #159748
    Purpose
    Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter). Selection of transgenic seeds by mTomato fluoresce.
    Depositor
    Wolfgang Lukowitz
    Article
    Angulo et al bioRxiv
    Insert
    gRNA scaffold
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Oct. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    159748-plasmid-map-sequence-id-313816

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  7. Polymerase-recruited click editor - pCMV-T7-PCV2-nCas9-CC(N6) (LM2705)

    Plasmid
    #208962
    Purpose
    Polymerase-recruited click editor comprised of PCV2 fused to nCas9 with a C-terminal N6 coiled coil (CC) domain, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    PCV2-XTEN-nSpCas9-BPNLS-CC(N6)
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A)
    Promoter
    CMV and T7
    Available since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    208962-plasmid-map-sequence-id-415796

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  8. pTUU

    Plasmid
    #159749
    Purpose
    Ubiquitin promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter). Selection of transgenic seeds by mTomato fluoresce.
    Depositor
    Wolfgang Lukowitz
    Article
    Angulo et al bioRxiv
    Insert
    gRNA scaffold
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Oct. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    159749-plasmid-map-sequence-id-313818

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  10. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-T7Sequenase (JO584)

    Plasmid
    #208955
    Purpose
    A variant CE1 construct with T7 Sequenase DNA polymerase, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    PCV2-XTEN-nSpCas9-BPNLS-Sequenase-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A)
    Promoter
    CMV and T7
    Available since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    208955-plasmid-map-sequence-id-415744

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  12. pGL

    Plasmid
    #119952
    Purpose
    Expresses GPPS and LimS, for the production of GPP and (S)-limonene, in Escherichia coli.
    Depositor
    Nigel Scrutton
    Article
    Jervis et al ACS Synth Biol. 2018 Dec 18. doi: 10.102
    Inserts
    gpps
    limS
    Use
    Tags
    Expression
    Bacterial
    Mutation
    N-terminal truncation of 56 amino acid residues (…
    Promoter
    Available since
    Feb. 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    119952-plasmid-map-sequence-id-235949

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  21. KCC2-pHext

    Plasmid
    #104076
    Purpose
    To visualize the expression and localization of potassium-chloride cotransporter type 2 (KCC2)
    Depositor
    Igor Medyna
    Article
    Friedel et al eNeuro. 2017 Jul 21;4(4). pii: ENEURO.00
    Insert
    KCC2 (b isoform) (Slc12a5 Rat)
    Use
    Tags
    pHluorine tag inserted in the second putative tra…
    Expression
    Mammalian
    Mutation
    EcoR1 site in rat KCC2 was removed by silent muta…
    Promoter
    UbC
    Available since
    Feb. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    104076-plasmid-map-sequence-id-199360

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  23. pAAV-hSyn-LMO3 (sbGLuc-VChR1-EYFP)

    Plasmid
    #114099
    Purpose
    fusion protein of Gaussia luciferase variant slow burn, Volvox channelrhodopsin-1, and enhanced yellow fluorescent protein for bioluminescent optogenetics
    Depositor
    Ute Hochgeschwender
    Article
    Berglund et al Proc Natl Acad Sci U S A. 2016 Jan 19;11
    Insert
    Gaussia luciferase, slow burn; Volvox Channelrhodopsin 1; EYFP
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hSyn
    Available since
    Aug. 6, 2019
    Availability
    Academic Institutions and Nonprofits only
    114099-plasmid-map-sequence-id-252568

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  26. pEMS1062

    Plasmid
    #29011
    Purpose
    The plasmid contains the Pleaides (Ple) MiniPromoter Ple155 derived from the human PCP2 gene and designed for expression in ON Bipolar cells of the retina.
    Depositor
    Elizabeth Simpson
    Article
    Korecki et al Gene Ther. 2021 Feb 2. pii: 10.1038/s414
    Insert
    Ple155-Cre/EGFP-NLS
    Use
    High copy number homologous recombination plasmid…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    MiniPromoter Ple155 from the human PCP2 gene
    Available since
    Feb. 22, 2012
    Availability
    Academic Institutions and Nonprofits only
    29011-plasmid-map-sequence-id-13417

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  27. Recruited polymerase expression - pCMV-T7-ePhi29(+exo)-CC(N5) (LM2982)

    Plasmid
    #208972
    Purpose
    Recruited ePhi29(+exo) DNA polymerase with a C-terminal N5 coiled coil (CC) domain, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    ePhi29(+exo)-BPNLS-CC(N5)
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    ePhi29(+exo)(M8R/V51A/M97T/G197D/E221K/Q497P/K512…
    Promoter
    CMV and T7
    Available since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    208972-plasmid-map-sequence-id-415827

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  32. pGEX6P1-human LIC1 C-terminal half

    Plasmid
    #74599
    Purpose
    used for expression in E. coli and purification. N-terminal GST tag and C-terminal Strep tag, aa 389-523
    Depositor
    Ron Vale
    Article
    Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554
    Insert
    human dynein light intermediate chain 1 amino acids 389-523 (DYNC1LI1 Human)
    Use
    Tags
    N-terminal GST and Strep II
    Expression
    Bacterial
    Mutation
    amino acids 389-523 of LIC1
    Promoter
    T7
    Available since
    May 2, 2016
    Availability
    Academic Institutions and Nonprofits only
    74599-plasmid-map-sequence-id-145961

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  33. pEMS1626

    Plasmid
    #29281
    Purpose
    The plasmid contains the Pleaides (Ple) MiniPromoter Ple155 derived from the human PCP2 gene and designed for expression in ON Bipolar cells of the retina.
    Depositor
    Elizabeth Simpson
    Article
    Korecki et al Gene Ther. 2021 Feb 2. pii: 10.1038/s414
    Insert
    Ple155-Intron-LacZ-NLS
    Use
    High copy number homologous recombination plasmid…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    MiniPromoter Ple155 from the human PCP2 gene
    Available since
    June 10, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits
    29281-plasmid-map-sequence-id-13696

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  34. R-Q111R-N122D

    Plasmid
    #167178
    Purpose
    Expresses the full Leptodactylus latrans Na+K+ATPase with ATP1A1-R variant with reverted Q111R and N122D mutations in Sf9 cells
    Depositor
    Susanne Dobler
    Article
    Mohammadi et al Curr Biol. 2021 Jun 21;31(12):2530-2538.
    Inserts
    ATP1A1-R-Q111R-N122D
    ATP1B1
    Use
    Tags
    Expression
    Bacterial and Insect
    Mutation
    Changed R at 111 to Q and changed D at 122 to N
    Promoter
    P10 and PH
    Available since
    Feb. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    167178-plasmid-map-sequence-id-334483

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  43. pAAV-CMV-dSa VP64 Neurog2

    Plasmid
    #99695
    Purpose
    Expresses dSa Cas9 fused to gold standard VP64 activator domain and Sa sgRNA for Neurog2 promoter, vector allows for activation of mouse Neurog2, can be packaged and delivered as AAV
    Depositor
    George Church
    Article
    Vora et al bioRxiv 2018. 298620
    Insert
    dCas9 and gRNA targeting Neurog2 (gRNA: GGTATATAAGGGGTTTTAAG) (Neurog2 Mouse, Synthetic)
    Use
    AAV and CRISPR
    Tags
    VP64
    Expression
    Mutation
    dead Cas9
    Promoter
    Available since
    Sept. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    99695-plasmid-map-sequence-id-194570

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  44. pLPC-L647RLaminA-CS

    Plasmid
    #69062
    Purpose
    encodes a lamin A mutant with Leucine 647 mutated to an Arginin and with a substitution of cysteine in CaaX-motif by a serine
    Depositor
    Gerardo Ferbeyre
    Article
    Moiseeva et al Cell Cycle. 2015 Aug 3;14(15):2408-21. d
    Insert
    LaminA (LMNA Human)
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Lamin A with Leucine 647 mutated to Arginine and …
    Promoter
    CMV
    Available since
    Dec. 17, 2015
    Availability
    Academic Institutions and Nonprofits only

  48. CmCitrine Lck w/ NosUTRs

    Plasmid
    #66785
    Purpose
    expression of fluorescent membranes in sea urchin primordial germ cells
    Depositor
    Amro Hamdoun
    Article
    Campanale et al Dev Dyn. 2014 Jul;243(7):917-27. doi: 10
    Insert
    Lck (LCK Human)
    Use
    Sea urchin, zebrafish, xenopus
    Tags
    3' UTR from sea urchin Nanos, 5' UTR fr…
    Expression
    Mutation
    Tandem repeat of LCK the N-terminal membrane targ…
    Promoter
    T7
    Available since
    July 24, 2015
    Availability
    Academic Institutions and Nonprofits only

  50. pBEST-p70a-UTR1-deGFP Y35TAG-6xHis-T500

    Plasmid
    #92225
    Purpose
    Expression of deGFP mutant Y35TAG for ncAA incoporation using the p70a promoter and UTR1
    Depositor
    Lital Alfonta
    Article
    Schlesinger et al ACS Synth Biol. 2017 Mar 9. doi: 10.1021
    Insert
    deGFP Y35TAG
    Use
    Synthetic Biology; Cell free protein synthesis (c…
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Residue Y35 mutated into TAG (Amber) stop codon f…
    Promoter
    p70a
    Available since
    Oct. 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    92225-plasmid-map-sequence-id-197052

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Showing: 7251 - 7300 of 18985 results