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177,170 results

Showing: 7351 - 7400 of 177170 results

  1. AIMTOR T757

    Plasmid
    #140828
    Purpose
    AIMTOR T757 contains a cytoplasmic mTOR Activity Reporter derived from hu ULK1 protein boxed between BRET-compatible entities (Ypet and Nanoluciferase) to measure mTOR activity in living cells
    Depositor
    Anne Bonnieu
    Publication
    Bouquier et al BMC Biol. 2020 Jul 3;18(1):81. doi: 10.1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    AIMTOR T757 (ULK1 Human)
    Tags
    NES Nuclear export signal
    Expression
    Mammalian
    Mutation
    The insert comprises only a small sequence of hum…
    Available since
    July 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    140828-plasmid-map-sequence-id-305141

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  3. pVE13300

    Plasmid
    #137715
    Purpose
    Expresses impLbCas12a in mammalian cells.
    Depositor
    Ervin Welker
    Publication
    Toth et al Nucleic Acids Res. 2020 Feb 28. pii: 576
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    impLbCas12a
    Tags
    3xHA, NLS, and SV40 NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    March 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    137715-plasmid-map-sequence-id-274023

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  12. pLX_TRC311

    Plasmid
    #113668
    Purpose
    Gateway destination vector for lentiviral expression
    Depositor
    John Doench
    Type
    Empty backbone
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available since
    Sept. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    113668-plasmid-map-sequence-id-220922

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  16. pET28a-HaloTag7-SnoopLigase

    Plasmid
    #105627
    Purpose
    Plasmid for recombinant bacterial expression of SnoopLigase, a protein that promotes the formation of an isopeptide bond between 2 peptide tags (SnoopTagJr and DogTag), with N-terminal HaloTag7
    Depositor
    Mark Howarth
    Publication
    Buldun et al J Am Chem Soc. 2018 Feb 28;140(8):3008-3
    Insert
    HaloTag7-SnoopLigase
    Expression
    Bacterial
    Promoter
    T7
    Available since
    March 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    105627-plasmid-map-sequence-id-207217

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  17. pCH1/RAD51o

    Plasmid
    #102562
    Purpose
    Express Human RAD51 protein codon optimzed for E.coli and the E.coli GorE Operon
    Depositor
    Maria Spies
    Publication
    Subramanyam et al Proc Natl Acad Sci U S A. 2016 Oct 11;11
    Inserts
    Homo sapiens RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae) (RAD51), transcript variant 1 (RAD51 Synthetic, Human)
    E. coli groE operon
    Expression
    Bacterial
    Mutation
    Codon optimized for E.coli Expression
    Promoter
    T7
    Available since
    Feb. 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    102562-plasmid-map-sequence-id-203161

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  18. pAAV.GFAP.eGFP.WPRE.hGH

    Plasmid
    #105549
    Purpose
    AAV expression of EGFP from GFAP promoter
    Depositor
    James M. Wilson
    Has service
    AAV5
    Insert
    eGFP
    Use
    AAV
    Expression
    Mammalian
    Promoter
    GFAP
    Available since
    Feb. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    8 Articles
    105549-plasmid-map-sequence-id-202447

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  25. pFC333

    Plasmid
    #87844
    Purpose
    AMA1 plasmid with Aspergillus optimized Cas9 and ble selection marker
    Depositor
    Uffe Mortensen
    Publication
    Nodvig et al PLoS One. 2015 Jul 15;10(7):e0133085. do
    Inserts
    Cas9
    ble (bleomycin resistance marker)
    Use
    CRISPR; Fungal expression
    Tags
    SV40 NLS
    Expression
    Bacterial
    Promoter
    Aspergillus nidulans tef1 promoter and Aspergillu…
    Available since
    May 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    5 Articles
    87844-plasmid-map-sequence-id-174620

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  35. pAAV-hSyn-FLEX-TVA-P2A-EGFP-2A-oG

    Plasmid
    #85225
    Purpose
    Expresses TVA, EGFP, and optimized Rabies G (oG) protein in a FLEX cassette
    Depositor
    John Naughton
    Inserts
    Optimized G Protein
    Enhanced GFP
    TVA (CD320 Chicken)
    Use
    AAV and Cre/Lox
    Promoter
    hSyn
    Available since
    Jan. 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    15 Articles
    85225-plasmid-map-sequence-id-170787

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  36. p2CT-His-MBP-Lbu_C2c2_R472A_H477A_R1048A_H1053A

    Plasmid
    #83485
    Purpose
    Bacterial expression of L. buccalis C2c2 CRISPR effector (double HEPN nuclease 1 and 2 inactive mutant).
    Depositor
    Jennifer Doudna
    Publication
    East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature
    Insert
    Lbu_C2c2_R472A_H477A_R1048A_H1053A
    Use
    CRISPR
    Tags
    His6-MBP
    Expression
    Bacterial
    Mutation
    R472A, H477A, R1048A, H1053A
    Available since
    Oct. 31, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    83485-plasmid-map-sequence-id-159341

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  39. pHLmMBP-8

    Plasmid
    #72347
    Purpose
    Mammalian expression of cytoplasmic mMBP TEV cleavage site-linked fusion proteins with C-terminal 6His-tag/Strep-tag II/HA-tag
    Depositor
    Luca Jovine
    Publication
    Bokhove et al J Struct Biol. 2016 Feb 3. 194:1-7. pii
    Type
    Empty backbone
    Tags
    6His-tag, HA-tag, Strep-tag II, and mMBP with C-t…
    Expression
    Mammalian
    Promoter
    CMV enhancer + chicken beta-actin promoter
    Available since
    Feb. 17, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    72347-plasmid-map-sequence-id-133755

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  44. pUV15-pHGFP

    Plasmid
    #70045
    Purpose
    encodes a pH-sensitive ratiometric GFP (pH-GFP), which allows for noninvasive measurements of intrabacterial pH in live cells
    Depositor
    Sabine Ehrt
    Publication
    Vandal et al Nat Med. 2008 Aug;14(8):849-54. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    phGFP
    Use
    Mycobacterium tuberculosis expression vector
    Promoter
    mycobacterial promoter Psmyc
    Available since
    Oct. 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    6 Articles
    70045-plasmid-map-sequence-id-488289

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  47. Vimentin - APEX2 in pECFP

    Plasmid
    #66170
    Purpose
    APEX2 fused to the C-terminus human vimentin
    Depositor
    Alice Ting
    Publication
    Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human Vimentin - APEX2 (VIM Synthetic, Human)
    Tags
    Flag epitope tag (DYKDDDDK)
    Expression
    Mammalian
    Mutation
    APEX2 contains 4 mutations relative to wt soybean…
    Promoter
    CMV
    Available since
    July 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    66170-plasmid-map-sequence-id-113682

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  48. pBUE411

    Plasmid
    #62200
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (OsU3 promoter), Bar resistance
    Depositor
    Qi-Jun Chen
    Publication
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Inserts
    gRNA scaffold
    zCas9
    Use
    Plant binary vector
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    maize codon optimized
    Promoter
    OsU3 and Ubi
    Available since
    April 29, 2015
    Availability
    Academic Institutions and Nonprofits only
    62200-plasmid-map-sequence-id-395472

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Showing: 7351 - 7400 of 177170 results