163,606 results

1. Puro-Cas9 donor

   Plasmid

   #58409

   Purpose

   Donor vector for genomic targeting of a Tetracycline-inducible Cas9 cassette to the human AAVS1/PPP1R12C locus

   Depositor

   Danwei Huangfu

   Article

   Gonzalez et al Cell Stem Cell. 2014 Jun 11. pii: S1934-

   Insert

   hSpCas9
   

   Use

   CRISPR

   Tags

   3xFlag, Nucleoplasmin NLS, and SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. mCherry-Rab5a-7

   Plasmid

   #55126

   Purpose

   Localization: Ras GTPase, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Insert

   Rab5a
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pB-TopFlash-NanoLucP

   Plasmid

   #204347

   Purpose

   TopFlash reporter

   Depositor

   Li-Fang Chu

   Article

   Conrad et al Stem Cell Reports. 2023 Dec 12;18(12):23

   Insert

   TopFlash-NanoLucP
   

   Use

   Luciferase

   Tags

   NanoLucP

   Expression

   Mutation

   Promoter

   miniP

   Available Since

   Jan. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLminP_Luc2P_RE38

   Plasmid

   #90378

   Purpose

   RELB - NFkB gene reporter

   Depositor

   Ramnik Xavier

   Article

   O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

   Insert

   Firefly luciferase
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   MAF

   Available Since

   Nov. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pShuttle-Flag-XBP1u

   Plasmid

   #63679

   Purpose

   Adenoviral shuttle vector for XBP1u expression

   Depositor

   Qingbo Xu, Lingfang Zeng

   Article

   Zeng et al Proc Natl Acad Sci U S A. 2009 May 19;10

   Insert

   XBP1 (XBP1 Human)
   

   Use

   Adenoviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   unspliced

   Promoter

   CMV

   Available Since

   March 23, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pMSCVII-AM

   Plasmid

   #162751

   Purpose

   Retroviral (MSCV) expression of ametrine fluorescent protein

   Depositor

   Maki Nakayama

   Article

   Landry et al Bio Protoc. 2021 Jan 20;11(2):e3883. doi

   Insert

   ametrine
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pEND-351_Cacnes_dCas9_CRISPRi

   Plasmid

   #225617

   Purpose

   Cutibacterium acnes replicative plasmid with dCas9 for CRISPRi. pBRESP36A-based vector optimized for reduced size and modular assembly, harbouring a medium-copy pBR322 E. coli ori (no ROP)

   Depositor

   Marc Güell

   Article

   Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

   Insert

   dCas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Codon optimized for Cutibacterium acnes

   Promoter

   Available Since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. FokI-dCas9

   Plasmid

   #52970

   Purpose

   Expresses Fok1 nuclease domain fused to catalytically inactive Cas9 DNA-binding domain in mammalian cells

   Depositor

   David Liu

   Article

   Guilinger et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

   Insert

   Fok1 fused to dCas9
   

   Use

   CRISPR

   Tags

   Fok1 nuclease (homodimeric wildtype) and NLS

   Expression

   Mammalian

   Mutation

   D10A and H840A in Cas9

   Promoter

   CMV

   Available Since

   May 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. scAAV-hIBA1a-GFP

   Plasmid

   #214146

   Purpose

   Self-complementary AAV vector packaging GFP under the human Iba1 promoter

   Depositor

   Chun-Li Zhang

   Article

   Serrano et al iScience. 2024 Aug 12;27(9):110706. doi:

   Insert

   GFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. SIV_Gag_Pol

    Plasmid

    #236243

    Purpose

    3rd generation SIV-based lentiviral packaging plasmid; Contains SIV Gag and Pol

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

    Insert

    SIV Gag and Pol
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pFUGW-FIRE-pHLy

    Plasmid

    #170774

    Purpose

    Ratiometric biosensor expressed with UbC promoter for probing lysosomal pH in mammalian cells

    Depositor

    Aimee Kao

    Article

    Chin et al ACS Sens. 2021 Jun 8. doi: 10.1021/acsse

    Insert

    LAMP1 (LAMP1 Human)
    

    Use

    Lentiviral

    Tags

    mCherry and mTFP1

    Expression

    Mammalian

    Mutation

    84 nucleotide Signal Sequence switched to the beg…

    Promoter

    hUbC

    Available Since

    Sept. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. phND2-N174

    Plasmid

    #31822

    Depositor

    Jerry Crabtree

    Article

    Yoo et al Nature. 2011 Jul 13. doi: 10.1038/nature

    Insert

    NeuroD2 (NEUROD2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 13, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. PGK-AAVS1ZFNR

    Plasmid

    #60915

    Purpose

    encodes zinc finger nuclease specific to AAVS1 locus (right)

    Depositor

    Paul Gadue

    Article

    Sim et al Methods Mol Biol. 2015 Jan 29.

    Insert

    AAVS1-ZFNR
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    PGK

    Available Since

    Feb. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA-SADB19L

    Plasmid

    #32632

    Depositor

    Edward Callaway

    Article

    Osakada et al Neuron. 2011 Aug 25;71(4):617-31.

    Insert

    SADB19L
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 26, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. dCas13d-dsRBD

    Plasmid

    #154938

    Purpose

    BPNLS-dRfxCas13d-dsRBD-BPNLS-HA

    Depositor

    Alice Ting

    Article

    Han et al Proc Natl Acad Sci U S A. 2020 Aug 24. p

    Insert

    BPNLS-dRfxCas13d-dsRBD-BPNLS-HA
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3 Flag-humanTRAF2 (1087)

    Plasmid

    #66931

    Purpose

    Mammalian Expression of TRAF2

    Depositor

    Michael Karin

    Insert

    TRAF2 (TRAF2 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pUC18T-mini-Tn7T-Gm-Pc-tdTomato

    Plasmid

    #199250

    Purpose

    Fluorescent labelling of bacterial cells with tdTomato

    Depositor

    Uwe Mamat

    Article

    Mamat et al Appl Environ Microbiol. 2023 May 17:e003

    Insert

    Codon-optimized tdTomato gene
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Codon optimization for Xanthomonadaceae

    Promoter

    Pc promoter from class III integron of Delftia a…

    Available Since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. Sp_PE

    Plasmid

    #212038

    Purpose

    Bacterial expression of the PE2 proteins for genome editing in E. coli

    Depositor

    Quanjiang Ji

    Article

    Zhang et al Nat Commun. 2024 Jan 27;15(1):825. doi:

    Insert

    PE2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    L-arabinose-inducible promoter PBAD

    Available Since

    April 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. FUGW U6 gLacZ dCas9-KRAB-T2a-GFP

    Plasmid

    #234883

    Purpose

    non-targeting CRISPRi control

    Depositor

    Johan Jakobsson

    Article

    Adami et al bioRxiv 2025.01.17.633315

    Insert

    L1HS gRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3-EGFP-Rac1-Q61L

    Plasmid

    #12981

    Depositor

    Gary Bokoch

    Article

    Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

    Insert

    Rac1 constitutively active (RAC1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Q61L Constitutively active

    Promoter

    Available Since

    Oct. 27, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. Lenti-LucOS

    Plasmid

    #22777

    Depositor

    Tyler Jacks

    Insert

    OVA-SIY
    

    Use

    Cre/Lox and Lentiviral

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCDNA3.1(+)-NEMOm

    Plasmid

    #189930

    Purpose

    Mammalian expression of calcium sensor with ultra-high dynamics and sensitivity

    Depositor

    Yubin Zhou

    Article

    Li et al Nat Methods. 2023 Jun;20(6):918-924. doi

    Insert

    NEMOs
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA-SADB19P

    Plasmid

    #32631

    Depositor

    Edward Callaway

    Article

    Osakada et al Neuron. 2011 Aug 25;71(4):617-31.

    Insert

    SADB19P
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 26, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCRY2PHR-mCherryN1

    Plasmid

    #26866

    Purpose

    Expresses CRY2(1-498)-mCherry fusion for use in light-inducible protein interaction modules

    Depositor

    Chandra Tucker

    Article

    Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

    Insert

    CRY2PHR (CRY2 Mustard Weed)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. YFVdelCME-mVenus

    Plasmid

    #179953

    Purpose

    pCCI vector encoding YFV-17D lacking C, prM, E proteins but with mVenus

    Depositor

    Wei Xu

    Article

    Li et al Nat Methods. 2021 Nov 25. pii: 10.1038/s

    Insert

    YFV-17D lacking C, prM, E proteins but with mVenus
    

    Use

    Transneuronal tracing

    Tags

    Expression

    Mutation

    Promoter

    SP6

    Available Since

    Feb. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLenti CMV TRE3G Neo GFP-Lamin A wildtype

    Plasmid

    #118709

    Purpose

    Lentiviral TET-ON inducible GFP-Lamin A wildtype

    Depositor

    Tom Misteli

    Article

    Kubben et al Methods. 2015 Sep 1. pii: S1046-2023(15)

    Insert

    GFP-Lamin A human wildtype (LMNA Human)
    

    Use

    Lentiviral; Tet-on inducible

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV TRE3G (TET-ON)

    Available Since

    Nov. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. PGK-AAVS1ZFNL

    Plasmid

    #60916

    Purpose

    encodes zinc finger nuclease specific to AAVS1 locus (left)

    Depositor

    Paul Gadue

    Article

    Sim et al Methods Mol Biol. 2015 Jan 29.

    Insert

    AAVS1-ZFNL
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    PGK

    Available Since

    Feb. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. TCARZ-CMV-Cherry-PAmin-GF3-R4A22

    Plasmid

    #203770

    Purpose

    For production of template RNA for use in PRINT, mCherry under CMV promoter

    Depositor

    Kathleen Collins

    Article

    Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

    Insert

    PRINT template RNA for mCherry
    

    Use

    Ivt template

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pDV002

    Plasmid

    #191823

    Purpose

    Plasmid for the transformation of the cyanobacterium Arthrospira platensis at the NS1 locus. No promoter. No terminator. Golden Gate compatible. Selection: in A. platensis: aadA; E. coli: AmpR

    Depositor

    Jim Roberts

    Article

    Tabakh et al STAR Protoc. 2023 Feb 8;4(1):102087. doi

    Type

    Empty backbone

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. NeonGreen-C1-ORP3

    Plasmid

    #220070

    Purpose

    overexpresses ORP3 with a neonGreen Fluorophore

    Depositor

    Gerry Hammond

    Article

    Doyle et al Contact (Thousand Oaks). 2024 Feb 7;7:25

    Insert

    OSBPL3 (OSBPL3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. T7-CMVtrans-FFLuc-polyA

    Plasmid

    #101156

    Purpose

    Template for T7 RNAP production of RNA transcript for expression of firefly luciferase in a eukaryotic mammalian cell free lysate.

    Depositor

    Marcel Bruchez

    Article

    Canady et al J Am Chem Soc. 2015 Aug 19;137(32):10268

    Insert

    Firefly luciferase
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    T7 promoter

    Available Since

    Jan. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pEF5B-FRT-cilia-APEX

    Plasmid

    #73186

    Purpose

    Expresses cilia-APEX in mammalian cells

    Depositor

    Maxence Nachury

    Article

    Mick et al Dev Cell. 2015 Nov 23;35(4):497-512. doi

    Insert

    cilia-APEX (Nphp3 Glycine max, Mouse)
    

    Use

    Tags

    internal GFP tag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available Since

    March 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pLPCX-VEcadTL

    Plasmid

    #45849

    Depositor

    Martin Schwartz

    Article

    Conway et al Curr Biol. 2013 May 14. pii: S0960-9822(

    Insert

    VE-cadherin (Cdh5 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pLenti-Lifeact-tdTomato

    Plasmid

    #64048

    Purpose

    Fluorescent reporter for F-actin labeling in living cells

    Depositor

    Weiping Han

    Article

    Lim et al Nat Commun. 2015 Jan 9;6:5951. doi: 10.1

    Insert

    Lifeact
    

    Use

    Lentiviral

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available Since

    July 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. CRISPR-StAR4B (Lenti)

    Plasmid

    #222691

    Purpose

    CRISPR-screening

    Depositor

    Ulrich Elling

    Article

    Uijttewaal et al Nat Biotechnol. 2024 Dec 16. doi: 10.103

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    PGK without BsmBI cutsite

    Promoter

    Available Since

    Dec. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. SARS-CoV-2 S HexaPro

    Plasmid

    #154754

    Purpose

    Mammalian expression vector for expression of the SARS-CoV-2 spike HexaPro variant

    Depositor

    Jason McLellan

    Article

    Hsieh et al Science. 2020 Sep 18;369(6510):1501-1505

    Insert

    Spike (HexaPro variant) (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    2X Strep-Tag II, 8X His tag, and HRV 3C cleavage …

    Expression

    Mammalian

    Mutation

    Ectodomain only (AAs 1-1208); 682-685 (furin site…

    Promoter

    Available Since

    July 1, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

37. pET302-6His-dCas9-Halo

    Plasmid

    #72269

    Purpose

    Encodes a Halo-labeled fusion of nuclease deficient Cas9

    Depositor

    Timothée Lionnet

    Article

    Deng et al Proc Natl Acad Sci U S A. 2015 Sep 22;11

    Insert

    His6-dCas9-Halo
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCDNA6A-EGFR WT

    Plasmid

    #42665

    Depositor

    Mien-Chie Hung

    Article

    Hsu et al J Biol Chem. 2007 Apr 6;282(14):10432-40

    Insert

    EGFR (EGFR Human)
    

    Use

    Tags

    6xHis and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pVC-FUS-WT

    Plasmid

    #175066

    Purpose

    expresses mVenus and mCherry tagged to FUS wildtype in mammalian cells

    Depositor

    Arnold Boersma

    Article

    Wan et al Cell Rep Methods. 2022 Mar 28;2(3):10018

    Insert

    FUS glycine rich protein (FUS Human)
    

    Use

    Tags

    mVenus + mCherry

    Expression

    Mammalian

    Mutation

    mVenus: C-Terminal deletion of 11 aa (GITLGMDELYK…

    Promoter

    CMV Promoter

    Available Since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. mito-mScarlet

    Plasmid

    #141153

    Purpose

    Fluorescent labeling of the mitochondrial matrix in mammalian cells

    Depositor

    Gia Voeltz

    Article

    Abrisch et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

    Insert

    COX4 (COX4 Budding Yeast)
    

    Use

    Tags

    mScarlet

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pCRISPathBrick

    Plasmid

    #65006

    Purpose

    E. coli vector for expression of S. pyogenes dCas9, tracrRNA, and nontargeting CRISPR array with BsaI site for inserting user-defined spacer-repeat bricks

    Depositor

    Mattheos Koffas

    Article

    Cress et al ACS Synth Biol. 2015 Mar 30.

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Constitutive native promoters

    Available Since

    May 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pAAV.hSynap.(cyto)iGlucoSnFR-mRuby2

    Plasmid

    #164510

    Purpose

    Green glucose sensor with mRuby2 fusion, cytosolic

    Depositor

    Loren Looger

    Article

    Keller et al bioRxiv (2019) 571422; doi: https://doi.

    Has Service

    AAV9

    Insert

    (Cyto)iGlucoSnFR-mRuby2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Synthetic Construct

    Promoter

    Synapsin

    Available Since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pCMV-PfV-Sapphire-IRES-DSRed

    Plasmid

    #116934

    Purpose

    Mammalian lenti vector for expression of the 40nm GEM and DSRed

    Depositor

    Liam Holt

    Article

    Delarue et al Cell. 2018 Jul 12;174(2):338-349.e20. do

    Insert

    Pfv
    

    Use

    Lentiviral

    Tags

    Sapphire

    Expression

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pLENTI_RPS10

    Plasmid

    #127129

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

    Insert

    RPS10 (RPS10 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    K24R- please see depositor comments

    Promoter

    Available Since

    Aug. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pAAVS1-NDi-CRISPRi (Gen2)

    Plasmid

    #73498

    Purpose

    Dox-inducible CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus

    Depositor

    Bruce Conklin

    Article

    Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

    Inserts

    dCas9-KRAB
    rtTA
    

    Use

    CRISPR

    Tags

    HA, KRAB, and NLS

    Expression

    Mammalian

    Mutation

    D10A, H840A (catalytically deactivated Cas9)

    Promoter

    CAG and TRE3G

    Available Since

    Dec. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pnEA-vH_His-TEV-SEPT2_SEPT6

    Plasmid

    #174497

    Purpose

    bacterial co-expression of human SEPT2 and of human SEPT6

    Depositor

    Manos Mavrakis

    Article

    Iv et al J Cell Sci. 2021 Jul 8. pii: 270842. doi

    Inserts

    SEPTIN2 (SEPTIN2 Human)
    SEPTIN6 (SEPTIN6 Human)
    

    Use

    Tags

    His6-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pKM-U6-reRNA-acceptor-2xBbsI

    Plasmid

    #205633

    Purpose

    Cloning and U6-drive expression of ISDra2 TnpB reRNAs

    Depositor

    Gerald Schwank

    Article

    Marquart et al Nat Methods. 2024 Sep 23. doi: 10.1038/s

    Insert

    ISDra2-TnpB reRNA with placeholder for guide cloning with BbsI
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Sept. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pXPR_206

    Plasmid

    #96920

    Purpose

    for SaCas9 knockout, lentiviral expression of S. aureus Cas9 (SauCas9) and gRNA scaffold

    Depositor

    David Root, John Doench

    Article

    Najm et al Nat Biotechnol. 2018 Feb;36(2):179-189.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. EB3-tdTomato

    Plasmid

    #50708

    Purpose

    expresses EB3-tdTomato fusion in mammalian cells for labeling the growing tips of MTs

    Depositor

    Erik Dent

    Article

    Merriam et al J Neurosci. 2013 Oct 16;33(42):16471-82.

    Insert

    EB3 (MAPRE3 Human)
    

    Use

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pcDNA3.1 VDAC1-Linker-GFP

    Plasmid

    #211734

    Purpose

    Expresses GFP tagged VDAC1 in mammalian cells. GFP is attached to the C-terminus of VDAC1 with a flexible linker.

    Depositor

    Roland Malli

    Article

    Pilic et al bioRxiv 2023.09.08.556841

    Insert

    Voltage-dependent anion channel 1 (VDAC1 )
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge