179,521 results
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Plasmid#102931PurposeLentiviral expression of a lysosomal tag (LAMP1-mRFP1-Flag)DepositorInsertLAMP1
UseLentiviralTagsmRFP-2xFlagPromoterubcAvailable sinceNov. 29, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSNAP-tag (T7) Vector
Plasmid#101137PurposeExpression of protein of interest with SNAP-Tag in bacterial cellsDepositorTypeEmpty backboneTagsSNAP-tag (SNAP26b)ExpressionBacterialAvailable sinceSept. 27, 2017AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pRN11
Plasmid#84455Purposeshuttle plasmid for sarA P1-mCherry expressionDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmCherry
UseShuttle vector e.coli-s.aureus, expression of sgf…Available sinceFeb. 17, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
MICU-APEX2
Plasmid#79057PurposeA C-terminal APEX2 fusion to the human mitochondrial calcium uptake 1 protein MICU1DepositorInsertC-terminal APEX2 fused MICU1 (MICU1 Human, Soybean)
UseLentiviralTagsFLAGExpressionBacterial and MammalianPromoterCMV immearly promotorAvailable sinceSept. 1, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pUCP20T-E2Crimson
Plasmid#78473PurposeBroad range bacterial fluorescence labelingDepositorInsertE2-Crimson
ExpressionBacterialAvailable sinceJune 14, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLH-sgRNA1-2XPP7
Plasmid#75390PurposesgRNA1-2XPP7DepositorInsertsgRNA1-2XPP7
UseCRISPR and LentiviralTagsnoExpressionMammalianPromoterU6Available sinceMay 25, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV hSyn FLExFRT mGFP-2A-Synaptophysin-mRuby
Plasmid#71761PurposeExpression of membrane-tethered EGFP and Synaptophysin-mRuby from Flp-expressing neuronsDepositorInsertsEGFP
Synaptophysin
UseAAVTagsPalmitoylation signal and mRubyAvailable sinceDec. 21, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA GNSTM-3-Flag-10-Lamp2b-HA
Plasmid#71293PurposeEncodes (N to C): GNSTM glycosylation motif, 3 residue spacer, Flag tag,10 residue spacer, Lamp2b (exosomal transmembrane protein), 3 residue spacer, HA tag.DepositorInsertLamp2b (LAMP2 Human)
TagsFLAG, GNSTM glycosylation motif, HA, and Lamp2 si…ExpressionMammalianPromoterCMVAvailable sinceDec. 4, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRetroX-Tight-MCS_PGK-GpNLuc
Plasmid#70185PurposeConstiutive mammalian expression of the bioluminescent GpNLuc LumiFluor reporter for single-cell analysis and sensitive optical imaging of living subjects.DepositorTypeEmpty backboneUseRetroviralExpressionMammalianPromoterPGKAvailable sinceDec. 2, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLV-STAT2
Plasmid#71451Purpose3rd generation lentiviral expression of STAT2DepositorAvailable sinceNov. 30, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDEST47-ARL8B-GFP
Plasmid#67404PurposeMammalian expression of hArl8b with C-term GFP tagDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertARL8B (ARL8B Human)
TagsGFPExpressionMammalianPromoterCMVAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEGFP-Anillin
Plasmid#68027PurposeFull length Anillin rendered RNAi-resistant) in mammalian expression vector pEGFP-C1DepositorInsertAnillin (ANLN Human)
TagseGFPExpressionMammalianMutationsilent mutations at 798 5'-TGCC TCTTTGAATAAA…PromoterCMVAvailable sinceSept. 11, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CAG-FLEx(FRT)-TC
Plasmid#67827PurposeFlp-dependent expression of TVA-mCherry fusion to mediate modifed rabies virus expression in cellsDepositorInsertTVA-mCherry fusion
UseAAVExpressionMammalianAvailable sinceAug. 10, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pFUGW-hGtACR1-EYFP
Plasmid#67795PurposeMammalian expression construct of human codon-adapted anion channelrhodopsin 1 from Guillardia theta fused in frame to EYFP via a NotI site in the pFUGW vector backboneDepositorInserthGtACR1
UseLentiviralTagsEYFPExpressionMammalianAvailable sinceAug. 6, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pFRT-TODestGFP_RNAseH1
Plasmid#65784Purposeexpresses GFP-tagged RNAseH1 in mammalian cellsDepositorAvailable sinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
TRIM33-GFP
Plasmid#65399PurposeGFP-tagged BRD protein, which can be used to be expressed in mammalian cells.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTRIM33 (TRIM33 Human)
TagsEmGFPExpressionMammalianMutationnonePromoterCMVAvailable sinceMay 13, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Tet-inducible mCherry reporter
Plasmid#64128PurposePhotoactivatable transcription system. mCherry reporter under the control of a tet-inducible promoterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmCherry
ExpressionMammalianPromoterTetOx13-CMVAvailable sinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCAG-VSVG
Plasmid#64084PurposeExpression vector for Vesicular Stomatitis Virus Indiana strain glycoprotein geneDepositorInsertVSV glycoprotein
ExpressionMammalianPromoterCAGAvailable sinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSBbi-GN
Plasmid#60517PurposeSB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: GFP and neomycin resistance geneDepositorTypeEmpty backboneUseTransposonExpressionMammalianPromoterEF1a/RPBSAAvailable sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGL3-Basic-8x-gRNA-eGFP
Plasmid#60718PurposeeGFP reporter containing 8 copies of a gRNA binding site for light-inducible dCas9 activationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsert8 copies of gRNA binding site (5'-AAAGGTCGAGAAACTGCAAA-3')
Available sinceFeb. 2, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCS2-Tol1.zf1
Plasmid#61388PurposemRNA synthesis of tol1 transposase codon modified for zebrafishDepositorInserttol1.zf1
UseZebrafish expressionPromotersp6Available sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEGFP-C3-Exo70
Plasmid#53761PurposepEGFP-C3 plasmid that expresses Exo70DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertExo70 (EXOC7 Human)
TagsGFPExpressionMammalianAvailable sinceJuly 22, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pWUR702
Plasmid#53079PurposeInducible TtAgo expressionDepositorInsertTtAgo
TagsStrep-II tagExpressionBacterialPromoterT7Available sinceJune 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCDNA-3xFLAG-Pontin
Plasmid#51635PurposeMammalian expression of 3xFLAG tagged pontin, N-terminal.DepositorAvailable sinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
DRH335: FCK-Optopatch1
Plasmid#51695PurposeCoexpression plasmid of QuasAr1 and CheRiff for all-optical electrophysiologyDepositorInsertOptopatch1
UseLentiviralExpressionMammalianPromoterCaMKIIaAvailable sinceApril 17, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAluYa5-neo-TET
Plasmid#51283Purposeexpresses Alu, a human SINE, using the pol III enhancer sequence of the 7SL gene and tagged with the self splicing intron neo cassetteDepositorInsertAluYa5
TagsneoTET cassette with a tetrahymena self-splicing …ExpressionMammalianPromoterupstream pol III enhancer sequence of the 7SL gen…Available sinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMDC32B-AtMIR390a-B/c
Plasmid#51776PurposePlant expression vector (2x35S) for direct cloning of artificial microRNAs into Arabidopsis thaliana MIR390a precursorDepositorPublicationInsertAtMIR390a-B/c
UsePlant expressionMutationA. thaliana MIR390a precursor sequence including …Available sinceApril 1, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mCherry-FKBP-MTM1
Plasmid#51614PurposeRecruitable PtdIns3P/PtdIns(3,5)P2 phosphataseDepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsTagsmCherryExpressionMammalianPromoterCMVAvailable sinceMarch 26, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Syn-ATP
Plasmid#51819PurposeOptical reporter of presynaptic ATPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertChimeric Synaptophysin-mCherry-Luciferase
ExpressionMammalianMutationWithin WT luciferase gene, changed Threonine 214 …PromoterCMVAvailable sinceMarch 13, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLKO.1-puro U6 sgRNA CAG
Plasmid#50927PurposeU6 driven sgRNA negative controlDepositorInsertnegative control sgRNA
UseCRISPR and LentiviralExpressionMammalianPromoterU6Available sinceJan. 22, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
M-NM-sgRNA
Plasmid#48673PurposeMammalian U6-driven sgRNA (NMm1) targeting GTCCCCTCCACCCCACAGTGDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsgRNA targeting GTCCCCTCCACCCCACAGTG, compatible with N. meningitidis Cas9, hU6 promoter
UseCRISPRPromoterhU6Available sinceOct. 22, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pACYCT2
Plasmid#45799DepositorTypeEmpty backboneExpressionBacterialAvailable sinceSept. 5, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
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pBAD/HisB-iRFP670
Plasmid#45453DepositorInsertiRFP670
ExpressionBacterialMutationT2A, S45T, V116I, D124E, M170L, K180M, D202L, I20…PromoteraraBADAvailable sinceJune 17, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEGFP VAMP4
Plasmid#42313DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertVAMP 4 (VAMP4 Human)
TagsEGFPExpressionMammalianPromoterCMVAvailable sinceMarch 1, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMXs-IP-EGFP-ULK1
Plasmid#38193DepositorInserta novel protein kinase structurally related to C. elegans UNC-51 (Ulk1 Mouse)
UseRetroviralTagsEGFPExpressionMammalianAvailable sinceNov. 27, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
2.2
Plasmid#34885DepositorInsertSindbis virus envelope protein
UseLentiviralExpressionMammalianMutationSGN UTR in E1 domainPromoterCMVAvailable sinceSept. 28, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDDGFP-A
Plasmid#40286DepositorInsertddGFP-A
TagsHis-tagExpressionBacterialAvailable sinceSept. 17, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pFRT/TO/HIS/FLAG/HA-HNRNPD
Plasmid#38066DepositorPublicationInsertHNRNPD heterogeneous nuclear ribonucleoprotein D (AU-rich element RNA binding protein 1, 37kDa) (HNRNPD Human)
TagsHIS/FLAG/HAExpressionMammalianAvailable sinceSept. 17, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-CaMKIIa-hChR2(E123T/T159C)-mCherry
Plasmid#35512PurposeAAV expression of CaMKIIa-driven hChR2 variant for ultrafast optogenetic control.DepositorHas serviceAAV2InserthChR2
UseAAVTagsmCherryExpressionMammalianMutationE123T and T159CPromoterCaMKIIaAvailable sinceApril 18, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by