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179,521 results

Showing: 6001 - 6040 of 179521 results

  2. pKC-4.06

    Plasmid
    #112084
    Purpose
    NMD sensor: pCMV-3XFLAG-Fluc-betaglobin (39PTC)
    Depositor
    James Inglese
    Publication
    Baird et al eLife. 2018 Mar 12;7. pii: 33178. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    3XFLAG firefly luciferase-beta-globin 39PTC (HBB Human, Photinus pyralis, Mouse)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    112084-plasmid-map-sequence-id-214510

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  8. pDG461

    Plasmid
    #100902
    Purpose
    SpCas9n (D10A Nickase mutant) with 2A-EGFP and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of DSBs with no off-target cuts.
    Depositor
    Paul Thomas
    Publication
    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do
    Inserts
    humanized CRISPR associated protein 9 Nickase
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    Use
    CRISPR and Mouse Targeting
    Tags
    3xFLAG and T2A-EGFP
    Expression
    Mammalian
    Mutation
    D10A mutant converts to Nickase
    Promoter
    CBh and U6
    Available since
    Dec. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    100902-plasmid-map-sequence-id-195323

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  28. GFP-GCN5

    Plasmid
    #65386
    Purpose
    GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.
    Depositor
    Kyle Miller
    Publication
    Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GCN5 (KAT2A Human)
    Tags
    EmGFP and V5
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available since
    May 14, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    65386-plasmid-map-sequence-id-159425

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  32. tol2-mpeg1-mcherry

    Plasmid
    #58935
    Purpose
    labels macrophages with mcherry
    Depositor
    Anna Huttenlocher
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mpeg1-mcherry
    Promoter
    mpeg1
    Available since
    Sept. 5, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    58935-plasmid-map-sequence-id-91295

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  35. pcDNA3.1-Hyg-NLS.EGFP.P2A.Cherry.CAAX

    Plasmid
    #52421
    Purpose
    Expresses a polyprotein cleaved by peptide 2A into nuclear GFP and membrane located mCherry
    Depositor
    Bertrand Collet
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Fusion NLS-EGFP-P2A-Cherry-CAAX
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    April 3, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    52421-plasmid-map-sequence-id-81252

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  38. M-ST1-sgRNA

    Plasmid
    #48672
    Purpose
    Mammalian U6-driven sgRNA (STm1) targeting GTCCCCTCCACCCCACAGTG
    Depositor
    George Church
    Publication
    Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgRNA targeting GTCCCCTCCACCCCACAGTG, compatible with S. thermophilus #1 Cas9, hU6 promoter
    Use
    CRISPR
    Promoter
    hU
    Available since
    Oct. 22, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    48672-plasmid-map-sequence-id-71846

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  39. M-ST1n-VP64

    Plasmid
    #48675
    Purpose
    Mammalian ST1-VP64 nuclease-null Cas9 activator expression, human optimized
    Depositor
    George Church
    Publication
    Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cas9-VP64, nuclease-null
    Use
    CRISPR
    Tags
    NLS and VP64
    Mutation
    nuclease-null (D9A D598A H599A N622A)
    Promoter
    CMV
    Available since
    Oct. 16, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    48675-plasmid-map-sequence-id-71850

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Showing: 6001 - 6040 of 179521 results