We narrowed to 9,660 results for: Gnas

1. pCAG-memRFP

   Plasmid

   #224564

   Purpose

   Ubiquitous expression plasmid, contains CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), MCS and membrane RFP reporter.

   Depositor

   Erica Hutchins

   Article

   Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

   Insert

   mRFP1
   

   Use

   Unspecified

   Tags

   Membrane localization signal

   Expression

   Mutation

   Codon optimized

   Promoter

   Available since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pTarget-Dual

   Plasmid

   #207884

   Purpose

   Carries the cognate target sites for the homing endonucleases I-OnuI and I-GpeMI.

   Depositor

   David Edgell

   Article

   Foo et al Nucleic Acids Res. 2024 Feb 28;52(4):206

   Inserts

   I-OnuI target site
   I-GpeMI target site
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3.1-39S Light chain

   Plasmid

   #216297

   Purpose

   Expresses anti-HER2 39S Light chain in mammalian cells. To be paired with 39S heavy chain to form the anti-HER2 39S Fab

   Depositor

   Mark Howarth

   Article

   Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

   Insert

   39S Light chain
   

   Use

   Affinity Reagent/ Antibody

   Tags

   Signal peptide

   Expression

   Mammalian

   Mutation

   Promoter

   T7 promoter

   Available since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3.1-MF3958 Light chain

   Plasmid

   #216304

   Purpose

   Expresses anti-HER2 MF3958 Light chain in mammalian cells. To be paired with MF3958 heavy chain to form the anti-HER2 MF3958 Fab

   Depositor

   Mark Howarth

   Article

   Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

   Insert

   MF3958 Light chain
   

   Use

   Affinity Reagent/ Antibody

   Tags

   Signal peptide

   Expression

   Mammalian

   Mutation

   Promoter

   T7 promoter

   Available since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-H2-18 Heavy chain-SpyTag003

   Plasmid

   #216307

   Purpose

   Expresses anti-HER2 H2-18 Heavy chain-SpyTag003 in mammalian cells. To be paired with H2-18 light chain to form the anti-HER2 H2-18 Fab

   Depositor

   Mark Howarth

   Article

   Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

   Insert

   H2-18 Heavy chain-SpyTag003
   

   Use

   Affinity Reagent/ Antibody

   Tags

   His6 tag, Signal peptide, and SpyTag003

   Expression

   Mammalian

   Mutation

   Promoter

   T7 promoter

   Available since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28a-SnoopTag-SpyTag-(AffiHER2)x3

   Plasmid

   #216281

   Purpose

   Three affibodies to HER2 linked in a chain for bacterial expression. SnoopTag and SpyTag allow isopeptide bond formation to their cognate Catcher

   Depositor

   Mark Howarth

   Article

   Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

   Insert

   SnoopTag-SpyTag-(AffiHER2)x3
   

   Use

   Affinity Reagent/ Antibody

   Tags

   His6, SnoopTag, and SpyTag

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. ClhN-pADH3-GFP-Pex3-4V5-APEX2-URA

   Plasmid

   #207064

   Purpose

   Expressing GFP-Pex3-4V5-APEX2 under ADH3 promoter. Insufficient signal for EM.

   Depositor

   Zhiping Xie

   Article

   Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

   Insert

   Pex3
   

   Use

   Tags

   4xV5-APEX2 and GFP

   Expression

   Yeast

   Mutation

   Promoter

   pADH3

   Available since

   June 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Rif1-Halo HRD

   Plasmid

   #207081

   Purpose

   Homologous recombination donor for insertion of a HaloTag at the C-terminus of the endogenous RIF1 locus.

   Depositor

   Jens Schmidt

   Article

   Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

   Insert

   HaloTag followed by a PolyA signal and PuroR cassette flanked by human RNF168 locus sequences
   

   Use

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   Promoter

   None

   Available since

   May 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. RGG-BcLOV-mCh-SOS

   Plasmid

   #208278

   Purpose

   Expresses RGG-BcLOV-SOS for optogenetic activation of Ras/Erk signaling. Includes an mCherry tag.

   Depositor

   Lukasz Bugaj

   Article

   Pal et al Proc Natl Acad Sci U S A. 2023 Aug 8;120

   Insert

   RGG-BcLOV-mCh-SOS
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   pCMV

   Available since

   Jan. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. Fus-BcLOV-mCh-EGFR

    Plasmid

    #208281

    Purpose

    Expresses Fus-BcLOV-EGFR for optogenetic activation of EGFR signaling. Includes an mCherry tag.

    Depositor

    Lukasz Bugaj

    Article

    Pal et al Proc Natl Acad Sci U S A. 2023 Aug 8;120

    Insert

    Fus-BcLOV-mCh-EGFR
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    pCMV

    Available since

    Jan. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pUC57-ITR2-H1-GFP sgRNA Merry Target 3- eCMV- SaSp-3XFLAG-SV40NLS-ITR2

    Plasmid

    #210743

    Purpose

    Coding for SaSp Cas9 alongside Merry sgRNA targeting GFP Target 3

    Depositor

    Vincent Dion

    Article

    Mangin et al bioRxiv 2023.10.24.563270

    Inserts

    SaSp Cas9
    Merry sgRNA GFP Target 3
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS, PolyA signal

    Expression

    Mammalian

    Mutation

    N-terminal Sa Cas9, C-terminal Sp Cas9

    Promoter

    H1 and eCMV

    Available since

    Jan. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pUC57-ITR2-H1-GFP sgRNA Target 3- eCMV- S.pyoegenes-3XFLAG-SV40NLS-ITR2

    Plasmid

    #210739

    Purpose

    Coding for Sp Cas9 alongside Sp sgRNA targeting GFP Target 3

    Depositor

    Vincent Dion

    Article

    Mangin et al bioRxiv 2023.10.24.563270

    Inserts

    Sp Cas9 (cas9 S pyogenes)
    Sp sgRNA GFP Target 3
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS, PolyA signal

    Expression

    Mammalian

    Mutation

    Promoter

    H1 and eCMV

    Available since

    Jan. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pUC57-ITR2-H1-GFP sgRNA Target 1- eCMV- S.pyoegenes-3XFLAG-SV40NLS-ITR2

    Plasmid

    #210737

    Purpose

    Coding for Sp Cas9 alongside Sp sgRNA targeting GFP Target 1

    Depositor

    Vincent Dion

    Article

    Mangin et al bioRxiv 2023.10.24.563270

    Inserts

    Sp Cas9 (cas9 S pyogenes)
    Sp sgRNA GFP Target 1
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS, PolyA signal

    Expression

    Mammalian

    Mutation

    Promoter

    H1 and eCMV

    Available since

    Jan. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pUC57-ITR2-H1-GFP sgRNA Merry Target 4- eCMV- SaSp-3XFLAG-SV40NLS-ITR2

    Plasmid

    #210744

    Purpose

    Coding for SaSp Cas9 alongside Merry sgRNA targeting GFP Target 4

    Depositor

    Vincent Dion

    Article

    Mangin et al bioRxiv 2023.10.24.563270

    Inserts

    SaSp Cas9
    Merry sgRNA GFP Target 4
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS, PolyA signal

    Expression

    Mammalian

    Mutation

    N-terminal Sa Cas9, C-terminal Sp Cas9

    Promoter

    H1 and eCMV

    Available since

    Jan. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pUC57-ITR2-H1-GFP sgRNA Gollum Target 3- eCMV- SaSp-3XFLAG-SV40NLS-ITR2

    Plasmid

    #210747

    Purpose

    Coding for SaSp Cas9 alongside Gollum sgRNA targeting GFP Target 3

    Depositor

    Vincent Dion

    Article

    Mangin et al bioRxiv 2023.10.24.563270

    Inserts

    SaSp Cas9
    Gollum sgRNA GFP Target 3
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS, PolyA signal

    Expression

    Mammalian

    Mutation

    N-terminal Sa Cas9, C-terminal Sp Cas9

    Promoter

    H1 and eCMV

    Available since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pUC57-ITR2-H1-GFP sgRNA Gollum Target 4- eCMV- SaSp-3XFLAG-SV40NLS-ITR2

    Plasmid

    #210748

    Purpose

    Coding for SaSp Cas9 alongside Gollum sgRNA targeting GFP Target 4

    Depositor

    Vincent Dion

    Article

    Mangin et al bioRxiv 2023.10.24.563270

    Inserts

    SaSp Cas9
    Gollum sgRNA GFP Target 4
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS, PolyA signal

    Expression

    Mammalian

    Mutation

    N-terminal Sa Cas9, C-terminal Sp Cas9

    Promoter

    H1 and eCMV

    Available since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pUC57-ITR2-H1-GFP sgRNA Gollum Target 2- eCMV- SaSp-3XFLAG-SV40NLS-ITR2

    Plasmid

    #210746

    Purpose

    Coding for SaSp Cas9 alongside Gollum sgRNA targeting GFP Target 2

    Depositor

    Vincent Dion

    Article

    Mangin et al bioRxiv 2023.10.24.563270

    Inserts

    SaSp Cas9
    Gollum sgRNA GFP Target 2
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS, PolyA signal

    Expression

    Mammalian

    Mutation

    N-terminal Sa Cas9, C-terminal Sp Cas9

    Promoter

    H1 and eCMV

    Available since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pUC57-ITR2-H1-GFP sgRNA Merry Target 1- eCMV- SaSp-3XFLAG-SV40NLS-ITR2

    Plasmid

    #210741

    Purpose

    Coding for SaSp Cas9 alongside Merry sgRNA targeting GFP Target 1

    Depositor

    Vincent Dion

    Article

    Mangin et al bioRxiv 2023.10.24.563270

    Inserts

    SaSp Cas9
    Merry sgRNA GFP Target 1
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS, PolyA signal

    Expression

    Mammalian

    Mutation

    N-terminal Sa Cas9, C-terminal Sp Cas9

    Promoter

    H1 and eCMV

    Available since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pUC57-ITR2-H1- sgCTG Bilbo -eCMV-CjSpD8A-3XFLAG-SV40 NLS-ITR2

    Plasmid

    #210749

    Purpose

    Coding for CjSp D8A alongside Bilbo sgRNA targeting CAG repeats

    Depositor

    Vincent Dion

    Article

    Mangin et al bioRxiv 2023.10.24.563270

    Inserts

    CjSp D8A Cas9
    Bilbo sgCAG
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS, PolyA signal

    Expression

    Mammalian

    Mutation

    D8A, N-terminal Cj Cas9, C-terminal Sp Cas9

    Promoter

    H1 and eCMV

    Available since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pUC57-ITR2-H1-GFP sgRNA Target 2- eCMV- S.pyoegenes-3XFLAG-SV40NLS-ITR2

    Plasmid

    #210738

    Purpose

    Coding for Sp Cas9 alongside Sp sgRNA targeting GFP Target 2

    Depositor

    Vincent Dion

    Article

    Mangin et al bioRxiv 2023.10.24.563270

    Inserts

    Sp Cas9 (cas9 S pyogenes)
    Sp sgRNA GFP Target 2
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS, PolyA signal

    Expression

    Mammalian

    Mutation

    Promoter

    H1 and eCMV

    Available since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pUC57-ITR2-H1-GFP sgRNA Target 4- eCMV- S.pyoegenes-3XFLAG-SV40NLS-ITR2

    Plasmid

    #210740

    Purpose

    Coding for Sp Cas9 alongside Sp sgRNA targeting GFP Target 4

    Depositor

    Vincent Dion

    Article

    Mangin et al bioRxiv 2023.10.24.563270

    Inserts

    Sp Cas9 (cas9 S pyogenes)
    Sp sgRNA GFP Target 4
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS, PolyA signal

    Expression

    Mammalian

    Mutation

    Promoter

    H1 and eCMV

    Available since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pUC57-ITR2-H1-GFP sgRNA Gollum Target 1- eCMV- SaSp-3XFLAG-SV40NLS-ITR2

    Plasmid

    #210745

    Purpose

    Coding for SaSp Cas9 alongside Gollum sgRNA targeting GFP Target 1

    Depositor

    Vincent Dion

    Article

    Mangin et al bioRxiv 2023.10.24.563270

    Inserts

    SaSp Cas9
    Gollum sgRNA GFP Target 1
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS, PolyA signal

    Expression

    Mammalian

    Mutation

    N-terminal Sa Cas9, C-terminal Sp Cas9

    Promoter

    H1 and eCMV

    Available since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pUC57-ITR2-H1-GFP sgRNA Merry Target 2- eCMV- SaSp-3XFLAG-SV40NLS-ITR2

    Plasmid

    #210742

    Purpose

    Coding for SaSp Cas9 alongside Merry sgRNA targeting GFP Target 2

    Depositor

    Vincent Dion

    Article

    Mangin et al bioRxiv 2023.10.24.563270

    Inserts

    SaSp Cas9
    Merry sgRNA GFP Target 2
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS, PolyA signal

    Expression

    Mammalian

    Mutation

    N-terminal Sa Cas9, C-terminal Sp Cas9

    Promoter

    H1 and eCMV

    Available since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCMV-NLS-MBP-rATOM(WDR5)

    Plasmid

    #209710

    Purpose

    Mammalian expression of r-ATOM biosensor for WDR5 with nuclear localization signal

    Depositor

    Stewart Loh

    Article

    Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

    Insert

    NLS-MBP-rATOM(WDR5)
    

    Use

    Tags

    NLS-MBP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pGP4G-REL2N91-ccdB

    Plasmid

    #188444

    Purpose

    Zea Mays Nuclear Auxin Signaling CoRepressor REL2N91 and pDEST

    Depositor

    Britney Moss

    Article

    Ramos Baez et al Plant Physiol. 2020 Apr;182(4):1713-1722

    Insert

    REL2N91/ccdB
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. KBB441

    Plasmid

    #185131

    Purpose

    Swi6 full length with L->A mutated in nuclear localization signal

    Depositor

    Ken Belanger

    Article

    Belanger et al BMC Mol Cell Biol. 2022 Feb 21;23(1):10.

    Insert

    SWI6
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    SWI6 deletion of amino acids 450-458

    Promoter

    Available since

    Aug. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCytERM-miRFP670nano3

    Plasmid

    #184674

    Purpose

    Signal anchor of rabbit cytochrome p450 and miRFP670nano3

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

    Insert

    CytERM-miRFP670nano3
    

    Use

    Tags

    miRFP670nano3

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. KBB394

    Plasmid

    #185086

    Purpose

    NMA111 with both nuclear localization signals mutated to alanines fused to GFP (mutagenesis of KBB280)

    Depositor

    Ken Belanger

    Article

    Belanger et al J Cell Sci. 2009 Nov 1;122(Pt 21):3931-4

    Insert

    NMA111
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Nma111 KKR 9-11 AAA; KRK 28-30 AAA

    Promoter

    Available since

    July 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCEP4_HA-Halo-cmyc-GRM3 C127A

    Plasmid

    #140458

    Purpose

    Mammalian expression plasmid for N-terminal Halo- and c-myc-tagged mGluR3 C127A

    Depositor

    Erik Procko

    Insert

    GRM3 (GRM3 Human)
    

    Use

    Tags

    Halo, Signal/leader sequence from influenza hemag…

    Expression

    Mammalian

    Mutation

    Cysteine 127 to alanine, Threonine 174 to alanine

    Promoter

    Available since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pVH1001-Tier2(ColE1 ori)

    Plasmid

    #169599

    Purpose

    Tier-2 expression vector (A1-pA::A2-pA::A3-pA). The tier-2 expression vector consists of a custom MCS cassette containing the three Tier-1 compatible acceptor cassettes (A1, A2, and A3) each with its own non-homologous polyadenylation (pA) signals in a minimal backbone (AmpR and ColE1 ori).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pJEP206-AAV-1.3CaMKII-MCS-pA.

    Plasmid

    #78319

    Purpose

    AAV vector backbone containing a 1.3CaMKIIa promoter followed by a multiple cloning site(MCS) followed by a poly-Adenylation Signal (pA)

    Depositor

    Jonathan Ploski

    Article

    Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

    Type

    Empty backbone

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    1.3CaMKIIa

    Available since

    March 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pJEP210-AAV-0.4CaMKII P-MCS-pA

    Plasmid

    #63084

    Purpose

    AAV2 vector backbone containing a 0.4CaMKIIa promoter followed by a multiple cloning site(MCS) followed by a poly-Adenylation Signal (pA)

    Depositor

    Jonathan Ploski

    Article

    Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

    Type

    Empty backbone

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    0.4αCaMKII

    Available since

    March 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pJEP222-AAV-CMV-GFP-pA

    Plasmid

    #62916

    Purpose

    AAV vector backbone designed to express GFP from a CMV promoter. It also contains a poly-Adenylation Signal (pA)

    Depositor

    Jonathan Ploski

    Article

    Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

    Insert

    Green Fluorescent protein (GFP)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    March 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pJEP220-AAV-0.5Synapsin-GFP-pA

    Plasmid

    #62920

    Purpose

    AAV vector backbone designed to express GFP from a 0.5Synapsin promoter. It also contains a poly-Adenylation Signal (pA)

    Depositor

    Jonathan Ploski

    Article

    Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

    Insert

    Green Fluorescent protein (GFP)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    0.5synapsin

    Available since

    March 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pJEP219-AAV-1.1Synapsin-GFP-pA

    Plasmid

    #62921

    Purpose

    AAV vector backbone designed to express GFP from a 1.1Synapsin promoter. It also contains a poly-Adenylation Signal (pA)

    Depositor

    Jonathan Ploski

    Article

    Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

    Insert

    Green Fluorescent protein (GFP)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    1.1Synapsin

    Available since

    March 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. CTAG_NLS-SV40

    Plasmid

    #136108

    Purpose

    CTAG Nuclear localiation signal SV40 for fusion with CDS12

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    CTAG_NLS-SV40
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

37. pcDNA3.1_hNKD2

    Plasmid

    #174329

    Purpose

    encodes human NKD2 cDNA with a C-terminal MycHis Tag.

    Depositor

    Sonal Srikanth

    Article

    Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:

    Insert

    Homo sapiens NKD inhibitor of WNT signaling pathway 2 (NKD2 Human)
    

    Use

    Tags

    MycHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pLyGo-Ec-2

    Plasmid

    #163128

    Purpose

    pLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding MalE signal peptide and the LyGo cassette (SapI-ccdB-SapI)

    Depositor

    Morten Norholm

    Article

    Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pAGM9121_Hsa-Serum Albumin SP

    Plasmid

    #153496

    Purpose

    Human Serum Albumin Signal Peptide Donor Plasmid

    Depositor

    Sylvestre Marillonnet

    Article

    Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

    Insert

    Serum Albumin SP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pAGM9121_Sce-Killer Protein SP

    Plasmid

    #153498

    Purpose

    S. cerevisiae Killer Signal Peptide Donor Plasmid

    Depositor

    Sylvestre Marillonnet

    Article

    Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

    Insert

    Killer Protein SP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pAGM9121_Cfo-CPO SP

    Plasmid

    #153500

    Purpose

    Caldariomyces (Leptoxyphium) fumago Chloroperoxidase Signal Peptide Donor Plasmid

    Depositor

    Sylvestre Marillonnet

    Article

    Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

    Insert

    Chloroperoxidase SP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pAGM9121_Cgl-UPO SP

    Plasmid

    #153501

    Purpose

    Chaetomium globosum Unspecific Peroxygenase Signal Peptide Donor Plasmid

    Depositor

    Sylvestre Marillonnet

    Article

    Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

    Insert

    Unspecific peroxygenase SP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pAGM9121_Cci-UPO SP

    Plasmid

    #153502

    Purpose

    Coprinopsis cinerea Unspecific Peroxygenase Signal Peptide Donor Plasmid

    Depositor

    Sylvestre Marillonnet

    Article

    Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

    Insert

    Unspecific peroxygenase SP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pAGM9121_Aae-UPO* SP

    Plasmid

    #153506

    Purpose

    Agrocybe aegerita Unspecific peroxygenase (UPO*) Signal Peptide Donor Plasmid

    Depositor

    Sylvestre Marillonnet

    Article

    Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

    Insert

    Unspecific peroxygenase* SP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pAGM9121_Mth-UPO SP

    Plasmid

    #153507

    Purpose

    Unspecific peroxygenase Signal Peptide Donor Plasmid

    Depositor

    Sylvestre Marillonnet

    Article

    Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

    Insert

    Unspecific peroxygenase SP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pAGM9121_Sce-Acid Phosphatase SP

    Plasmid

    #153494

    Purpose

    S. cerevisiae Acid Phosphatase Signal Peptide Donor Plasmid

    Depositor

    Sylvestre Marillonnet

    Article

    Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

    Insert

    Acid Phosphatase SP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pAGM9121_Gma-UPO SP

    Plasmid

    #153495

    Purpose

    Galerina marginata Unspecific Peroxygenase Signal Peptide Donor Plasmid

    Depositor

    Sylvestre Marillonnet

    Article

    Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

    Insert

    Unspecific peroxygenase SP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pAGM9121_Mro-UPO SP

    Plasmid

    #153499

    Purpose

    Marasmius rotula Unspecific Peroxygenase Signal Peptide Donor Plasmid

    Depositor

    Sylvestre Marillonnet

    Article

    Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

    Insert

    Unspecific peroxygenase SP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pEarleyGate100-pSpoA-DsRed-Express2-FKBP(LV)(C22V)

    Plasmid

    #140169

    Purpose

    Expresses a regulatable secretory cargo (without an ER export signal) for Arabidopsis thaliana

    Depositor

    Benjamin Glick

    Article

    Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

    Insert

    pSpoA-DsRed-Express2-FKBP(LV)(C22V)
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV 35S

    Available since

    May 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pCRII-TOPO CMV-cGFP-SV40 pA + Laccase2 Exon 2

    Plasmid

    #91802

    Purpose

    Expresses the Laccase2 circular RNA when the upstream SV40 poly(A) signal fails to be used

    Depositor

    Jeremy Wilusz

    Article

    Liang et al Methods. 2021 Apr 18. pii: S1046-2023(21

    Insert

    Coral Green Fluorescent Protein (cGFP)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge