8,883 results

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pEGFP-GIT1

Plasmid

#15226

Depositor

Rick Horwitz

Article

Manabe et al J Cell Sci. 2002 Apr 1. 115(Pt 7):1497-5

Insert

G protein coupled receptor kinase-interacting protein 1 (GIT1 Human)

Tags

GFP

Expression

Mammalian

Mutation

L425V, E476K and L583P

Available Since

Sept. 13, 2007

Availability

Academic Institutions and Nonprofits only

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PKC iota WT

Plasmid

#21252

Depositor

Bernard Weinstein

Article

Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

Insert

PKC iota (PRKCI Human)

Tags

HA

Expression

Mammalian

Available Since

Sept. 8, 2009

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn1-mRuby2-GSG-P2A-GCaMP6f-WPRE-pA

Plasmid

#50943

Purpose

Bicistronic vector expressing mRuby2 and GCaMP6f from a single open reading frame.

Depositor

Tobias Bonhoeffer, Mark Huebener, Tobias Rose

Article

Rose et al Science. 2016 Jun 10;352(6291):1319-22.

Insert

mRuby2-P2A-GCaMP6f

Use

AAV

Promoter

hSyn1

Available Since

March 25, 2014

Availability

Academic Institutions and Nonprofits only

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pCI HA NEDD4L DD

Plasmid

#27001

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

NEDD4L (NEDD4L Human)

Tags

HA

Expression

Mammalian

Mutation

C962A

Available Since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only
MDH1-PGK-GFP_2.0

Plasmid

#11375

Purpose

Retroviral construct for miRNA expression

Depositor

Chang-Zheng Chen

Article

Chen et al Science. 2004 Jan 2. 303(5654):83-6.

Type

Empty backbone

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Aug. 3, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pGBT9-NF1-GRD

Plasmid

#19993

Depositor

Fuyuhiko Tamanoi

Article

Morcos et al Mol Cell Biol. 1996 May . 16(5):2496-503

Insert

Neurofibromatosis type I (NF1 Human)

Expression

Yeast

Available Since

Jan. 28, 2009

Availability

Academic Institutions and Nonprofits only

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CHOP::GFP

Plasmid

#21898

Depositor

David Ron

Article

Novoa et al J Cell Biol. 2001 May 28. 153(5):1011-22

Insert

CHOP (Ddit3 Mouse)

Use

Non-viral

Tags

EGFP

Available Since

Sept. 17, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pST1374-N-NLS-flag-linker-Cas9-H840A

Plasmid

#51129

Purpose

Cas9 nickase optimized for nuclear import.

Depositor

Xingxu Huang

Article

Shen et al Nat Methods. 2014 Mar 2. doi: 10.1038/nm

Insert

Cas9 nickase

Use

CRISPR

Tags

Flag and NLS

Expression

Mammalian

Mutation

H840A

Promoter

CMV

Available Since

March 7, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
p2CT-His-MBP-Lbu_C2c2_R472A_H477A_R1048A_H1053A

Plasmid

#83485

Purpose

Bacterial expression of L. buccalis C2c2 CRISPR effector (double HEPN nuclease 1 and 2 inactive mutant).

Depositor

Jennifer Doudna

Article

East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

Insert

Lbu_C2c2_R472A_H477A_R1048A_H1053A

Use

CRISPR

Tags

His6-MBP

Expression

Bacterial

Mutation

R472A, H477A, R1048A, H1053A

Available Since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pWZL Neo Myr Flag PRKAA1

Plasmid

#20595

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

PRKAA1 (PRKAA1 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pAAV-EF1a-FLEX-GT

Plasmid

#26198

Depositor

Edward Callaway

Article

Wall et al Proc Natl Acad Sci U S A. 2010 Dec 14;10

Insert

Enhanced green fluorescent protein, TVA (eGFP TVA is a mouse codon optimized version of an avian gene., Mouse)

Use

AAV and Cre/Lox

Mutation

TVA mammalian codon optimized

Available Since

Sept. 16, 2010

Availability

Academic Institutions and Nonprofits only

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pAAV-nEF Con/Fon hChR2(H134R)-EYFP

Plasmid

#55644

Purpose

Cre-on/Flp-on ChR2-EYFP under the short Ef1a promoter

Depositor

Karl Deisseroth

Article

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Insert

ChR2(H134R)-EYFP

Use

AAV; Cre on/flp on chr2-eyfp

Tags

EYFP

Expression

Mammalian

Promoter

nEF

Available Since

Aug. 11, 2014

Availability

Academic Institutions and Nonprofits only

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GAK-pmCherryN1

Plasmid

#27695

Depositor

Christien Merrifield

Article

Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

Insert

GAK (Gak Mouse)

Tags

mCherry

Expression

Mammalian

Available Since

May 4, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA-PACT

Plasmid

#15667

Depositor

Dong-Yan Jin

Article

Kok et al J Biol Chem. 2007 Jun 15. 282(24):17649-

Insert

PACT (PRKRA Human)

Expression

Mammalian

Available Since

Sept. 19, 2007

Availability

Academic Institutions and Nonprofits only
pEGFP-C3-hYAP2

Plasmid

#17844

Depositor

Marius Sudol

Article

Basu et al Mol Cell. 2003 Jan . 11(1):11-23.

Insert

YAP (YAP1 Human)

Tags

EGFP

Expression

Bacterial and Mammalian

Available Since

May 29, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
p4xDRE-Luc

Plasmid

#16534

Depositor

Bert Vogelstein

Article

He et al Cell. 1999 Oct 29. 99(3):335-45.

Insert

4xDRE

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

May 12, 2010

Availability

Academic Institutions and Nonprofits only
pBABE puro mouse IRS-2 myc

Plasmid

#11373

Depositor

Ronald Kahn

Article

Tsuruzoe et al Mol Cell Biol. 2001 Jan . 21(1):26-38.

Insert

IRS-2 (Irs2 Mouse)

Use

Retroviral

Tags

myc

Expression

Mammalian

Available Since

May 25, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
EGFR G719S

Plasmid

#11013

Purpose

Retroviral construct for expressing human EGFR with G719S mutation in human cells

Depositor

Matthew Meyerson

Article

Greulich et al PLoS Med. 2005 Oct 4. 2(11):e313.

Insert

EGFR G719S (EGFR Human)

Use

Retroviral

Expression

Mammalian

Mutation

G719S

Available Since

Dec. 2, 2005

Availability

Academic Institutions and Nonprofits only
pMXs-OLIG2

Plasmid

#32933

Depositor

Kevin Eggan

Article

Son et al Cell Stem Cell. 2011 Sep 2;9(3):205-18.

Insert

OLIG2 (OLIG2 Human)

Use

Retroviral

Expression

Mammalian

Promoter

5'LTR

Available Since

Nov. 30, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pAG425GAL-ccdB

Plasmid

#14153

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Gateway destination

Expression

Yeast

Available Since

March 16, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pCEP4YPet-MAMM

Plasmid

#14032

Depositor

Patrick Daugherty

Article

Nguyen et al Nat Biotechnol. 2005 Mar;23(3):355-60. d

Insert

YPet

Expression

Mammalian

Mutation

Mammalian optimized YPet

Available Since

Feb. 14, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223 SARS-CoV-2 ORF6

Plasmid

#141275

Purpose

Gateway-compatible Entry vector, with insert of ORF6 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

ORF6 (ORF6 SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway-compatible entry vector

Mutation

Many synonymous changes due to codon optimization

Available Since

April 1, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pQLinkG2

Plasmid

#13671

Depositor

Konrad Buessow

Article

Scheich et al Nucleic Acids Res. 2007;35(6):e43. doi:

Insert

multi cloning site

Use

Co-expression

Tags

GST, TEV, and attB1

Expression

Bacterial

Available Since

April 6, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pLOVE-Klf4

Plasmid

#15950

Depositor

Miguel Ramalho-Santos

Article

Blelloch et al Cell Stem Cell. 2007 Sep 13;1(3):245-7.

Insert

Kruppel-like factor 4 (Klf4 Mouse)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

The recombination reaction required to clone &quo…

Available Since

Nov. 7, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pBiFC-bFOSDelataZipVC155

Plasmid

#22014

Depositor

Chang-Deng Hu

Article

Shyu et al Biotechniques. 2006 Jan . 40(1):61-6.

Insert

bFosDelataZIPVC155

Tags

HA

Expression

Mammalian

Mutation

deletion of residues 179-193 in bFos(118-211)

Available Since

Sept. 28, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
SpyLigase

Plasmid

#51722

Purpose

Plasmid for recombinant expression in bacteria of SpyLigase. The Howarth lab has created two improved peptide ligation enzymes. See depositor comments below!

Depositor

Mark Howarth

Article

Fierer et al Proc Natl Acad Sci U S A. 2014 Mar 17.

Insert

SpyLigase

Tags

His6 Tag

Expression

Bacterial

Promoter

T7

Available Since

May 1, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
HA-FOXO3a TM-ER

Plasmid

#8353

Depositor

Michael Greenberg

Article

Tran et al Science 2002 Apr 19;296(5567):530-4.

Insert

FOXO3a (FOXO3 Human)

Tags

HA (YDVPDYASL)

Expression

Mammalian

Mutation

T32A, S253A, S315A, mouse estrogen receptor (G525…

Available Since

Feb. 13, 2006

Availability

Academic Institutions and Nonprofits only
pCS2+8

Plasmid

#34931

Depositor

Amro Hamdoun

Article

Gokirmak et al J Biol Chem. 2012 Nov 2.

Type

Empty backbone

Use

Sea urchin, zebrafish, xenopus

Expression

Mammalian

Promoter

SP6

Available Since

Nov. 30, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pJSC173 - Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9) variant

Plasmid

#101218

Purpose

Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9) variant

Depositor

Jennifer Doudna, Keith Joung

Article

Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

Insert

SpCas9 variant N692A/M694A/Q695A/H698A

Tags

10x His, MBP, and TEV site

Expression

Bacterial

Mutation

N692A, M694A, Q695A and H698A

Promoter

T7

Available Since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pNW55

Plasmid

#22988

Purpose

Expression of miRNA precursor for the osa-MIR528 locus in rice.

Depositor

Detlef Weigel

Article

Warthmann et al PLoS One. 2008 . 3(3):e1829.

Insert

osa-MIR528

Use

RNAi; Plant gene inactivation

Expression

Plant

Available Since

Jan. 27, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
Flag-HA-COPS6

Plasmid

#22542

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

COPS6 (COPS6 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available Since

June 18, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pMRX-IP/SECFP-hATG16A1

Plasmid

#58994

Purpose

Expresses SECFP-hATG16A1 in mammalian cells

Depositor

Noboru Mizushima

Article

Koyama-Honda et al Autophagy. 2013 Oct;9(10):1491-9. doi: 1

Insert

Homo sapiens autophagy related 16-like 1 (S. cerevisiae) (ATG16L1 Human)

Use

Retroviral

Tags

SECFP

Available Since

Aug. 4, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3 myc card9

Plasmid

#16253

Depositor

William Kaelin

Article

Yang et al Mol Cell. 2007 Oct 12. 28(1):15-27.

Insert

card9 (CARD9 Human)

Tags

myc

Expression

Mammalian

Available Since

Nov. 21, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3B-1081

Plasmid

#18759

Depositor

Clark Distelhorst

Article

Wang et al Oncogene. 2006 Mar 23. 25(13):1903-13.

Insert

thioredoxin-interacting protein promoter (Txnip Mouse)

Use

Luciferase

Available Since

Aug. 4, 2008

Availability

Academic Institutions and Nonprofits only
Cav1-mRFP

Plasmid

#14434

Depositor

Ari Helenius

Article

Tagawa et al J Cell Biol. 2005 Aug 29. 170(5):769-79.

Insert

Caveolin-1 (CAV1 Canis familalis)

Tags

mRFP

Expression

Mammalian

Available Since

March 23, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223-K-RAS V12

Plasmid

#31200

Purpose

Gateway donor vector containing KRAS G12V.

Depositor

David Root

Article

Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

Insert

K-Ras (KRAS Human)

Use

Gateway donor vector

Mutation

G12V constitutively active

Available Since

June 24, 2011

Availability

Academic Institutions and Nonprofits only
FU-tet-o-hSox2

Plasmid

#19779

Depositor

Konrad Hochedlinger

Article

Maherali et al Cell Stem Cell. 2008 Sep 11. 3(3):340-5.

Insert

Sox2 (SOX2 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pJBEI-6411

Plasmid

#47050

Purpose

BglBrick plasmid (=pBbB8k-P450) coding for a cytochrome P450 and its associated reductases to oxidize limonene to perillyl alcohol in E. coli

Depositor

Taek Soon Lee

Article

Alonso-Gutierrez et al Metab Eng. 2013 May 29;19C:33-41. doi: 1

Insert

ahpG-ahpH-ahpI coding for cytochrome P450 (CYP153A6) and its associated ferredoxin and ferredoxin reductase

Use

Synthetic Biology; Bglbrick

Expression

Bacterial

Promoter

Pbad

Available Since

Sept. 12, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pGRG37

Plasmid

#16667

Depositor

Nancy Craig

Article

McKenzie et al BMC Microbiol. 2006 Apr 28;6:39. doi: 10

Insert

mTn7::Gateway cassette

Expression

Bacterial

Available Since

Jan. 10, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
p5953 pcDNA-Xpress-His-USP15

Plasmid

#23216

Depositor

Peter Howley

Article

Vos et al J Virol. 2009 Sep . 83(17):8885-92.

Insert

USP15 (USP15 Human)

Tags

His and Xpress

Expression

Mammalian

Available Since

April 7, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
mKate1.31-pBAD

Plasmid

#54827

Purpose

Localization: Protein Expression Vector , Excitation: 588, Emission: 635. Also known as mKate2.

Depositor

Michael Davidson

Article

Shcherbo et al Biochem J. 2009 Mar 15;418(3):567-74. do

Type

Empty backbone

Tags

6xHis and mKate1.31 (aka mKate2)

Expression

Bacterial

Available Since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pPSmOrange2-N1

Plasmid

#34958

Depositor

Vladislav Verkhusha

Article

Subach et al J Am Chem Soc. 2012 Aug 17.

Insert

PSmOrange2

Expression

Mammalian

Mutation

R17H, R36H, F64I, Q194L, A224S, G226A (mutations …

Available Since

Aug. 20, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
MLV Gag-YFP

Plasmid

#1813

Depositor

Walther Mothes

Article

Sherer et al Traffic 2003 Nov;4(11):785-801.

Insert

Gag (gag MuLV)

Tags

YFP

Expression

Mammalian

Available Since

May 19, 2005

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE-CMV-Rheb(S16H)-IRES-eGFP-W

Plasmid

#32520

Depositor

Mustafa Sahin

Article

Nie et al Nat Neurosci. 2010 Feb;13(2):163-72. Epu

Insert

Constitutively active Rheb (S16H) (Rheb Rat)

Use

Lentiviral

Expression

Mammalian

Mutation

S16H to make constitutively active

Promoter

CMV

Available Since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pETZZtevPP7CP(C68A,C73A)

Plasmid

#27548

Depositor

Kathleen Collins

Article

Hogg et al RNA. 2007 Jun . 13(6):868-80.

Insert

Pseudomonas phage PP7 coat protein (cp Pseudomonas phage PP7)

Tags

TEV protease cleavage site, six histidine tag, an…

Expression

Bacterial

Mutation

changed two cysteine to alanine (C68A,C73A) to in…

Available Since

March 18, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA-GATA2

Plasmid

#1287

Depositor

Gokhan Hotamisligil

Article

Tong et al Science 2000 Oct 6;290(5489):134-8.

Insert

GATA2 (GATA2 Human)

Expression

Mammalian

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
pBAD/HisB-PAmCherry1

Plasmid

#31931

Depositor

Vladislav Verkhusha

Article

Subach et al Nat Methods. 2009 Feb;6(2):153-9. Epub 2

Insert

PAmCherry1

Tags

His

Expression

Bacterial

Mutation

E26V/A58T/K69N/L84F/N99K/S148L/I165V/Q167P/L169V/…

Promoter

araB

Available Since

Sept. 7, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pCBFRE-luc

Plasmid

#26897

Depositor

Nicholas Gaiano

Article

Yu et al Cell Stem Cell. 2008 May 8. 2(5):461-71.

Insert

CBFRE

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Available Since

Dec. 20, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA-flag-mKdm4d-polyA

Plasmid

#61553

Purpose

For mRNA synthesis or expression in mammalian cells

Depositor

Yi Zhang

Article

Matoba et al Cell. 2014 Nov 6;159(4):884-95. doi: 10.

Insert

Kdm4d (Kdm4d Mouse)

Tags

flag

Expression

Mammalian

Promoter

CMV and T7

Available Since

Feb. 23, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pBABE-hLin28B

Plasmid

#26358

Depositor

George Daley

Article

Viswanathan et al Nat Genet. 2009 Jul . 41(7):843-8.

Insert

lin-28 homolog B (LIN28B Human)

Use

Retroviral

Expression

Mammalian

Mutation

None

Available Since

Nov. 3, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge

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8,883 results