163,606 results

1. FLAG-SNAP29

   Plasmid

   #45915

   Depositor

   Noboru Mizushima

   Article

   Itakura et al Cell. 2012 Dec 7;151(6):1256-69. doi: 10

   Insert

   synaptosomal-associated protein, 29kDa (SNAP29 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMSCV-IRES-mCherry FP

   Plasmid

   #52114

   Purpose

   a bicistronic IRES-mCherry containing retroviral vector

   Depositor

   Dario Vignali

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   IRES-mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pT3TS-spCas9 ABE-Umax-ex2-rest1

   Plasmid

   #222143

   Purpose

   Adenine base editor with modified TadA variant for extended window and restricted A-to-G base editing in zebrafish

   Depositor

   Gaurav Varshney

   Article

   Qin et al Nat Commun. 2024 Jul 4;15(1):5613. doi:

   Insert

   Modified TadA-nSpCas9
   

   Use

   CRISPR; Zebrafish expression

   Tags

   Bipartite NLS

   Expression

   Mutation

   Promoter

   T3

   Available Since

   July 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCHW101020

   Plasmid

   #222454

   Purpose

   Level 1, Position 2 containing 35S::CUP2-p65::NOS.

   Depositor

   Chih-Hang Wu

   Article

   Chiang et al bioRxiv 2024.03.23.586378

   Insert

   35S::CUP2-p65::NOS (CUP2 )
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   no

   Promoter

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRetroX-TRE3G_NINJ1

   Plasmid

   #201181

   Purpose

   Dox-inducible expression of NINJ1 gene in mammalian cells

   Depositor

   Jonathan Kagan

   Article

   Borges et al Elife. 2022 Dec 5;11:e78609. doi: 10.755

   Insert

   Ninjurin 1 (Ninj1 Mouse)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mutation

   Promoter

   TRE3G

   Available Since

   May 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. RpH-ILV-3xALFA

   Plasmid

   #225135

   Purpose

   RpH-ILV probe strongly colocalises with lysosomal markers.

   Depositor

   Massimiliano Stagi

   Article

   Cheetham-Wilkinson et al Sci Adv. 2025 Jan 3;11(1):eadr7325. doi:

   Insert

   TMEM192 (TMEM192 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. EF.CMV.RFP

   Plasmid

   #17619

   Depositor

   Linzhao Cheng

   Article

   Yu et al Mol Ther. 2003 Jun . 7(6):827-38.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 27, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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8. PB-TRE-EGFP-EF1a-rtTA

   Plasmid

   #104454

   Purpose

   PiggyBac plasmid expressing EGFP under the control of a doxycycline-inducible promotor

   Depositor

   Volker Busskamp

   Article

   Sauter et al Methods Mol Biol. 2019;1942:101-121. doi

   Inserts

   enhanced green fluorescent protein
   reverse tetracycline-controlled transactivator 3
   

   Use

   Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pU6_HEK3+1T>A_(PP7-C4-Q1)

   Plasmid

   #232437

   Purpose

   pegRNA with optimized 3' modifications to facilitate a +1 T to A prime edit in the HEK3 locus

   Depositor

   Gil Westmeyer

   Article

   Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.

   Insert

   3'-PP7-tagged pegRNA for rd12 correction driven by human U6 promoter
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available Since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-PRB

    Plasmid

    #89130

    Purpose

    mammalian expression of full-length human progesterone receptor-B in pcDNA3, can be used to make stable cell line, select G418

    Depositor

    Elizabeth Wilson

    Article

    Su et al J Biol Chem. 2012 Oct 5;287(41):34809-24

    Insert

    human progesterone receptor-B (PGR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. CROP-seq-opti-dsRed

    Plasmid

    #201999

    Purpose

    Based on CROP-seq-opti (addgene #106280), we replaced the PURO resistance gene with a dsRed.

    Depositor

    Daniel Geschwind

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    dsRed

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    June 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti-sgRNA

    Plasmid

    #71409

    Purpose

    There is no gene/insert. It is a backbone plasmid that others can use to insert sgRNAs of interest. The cloning site for this BsmBI

    Depositor

    David Sabatini, Eric Lander

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. PAmCherry1-N1-Tubbyc

    Plasmid

    #202677

    Purpose

    Photoactivatable lipid biosensor

    Depositor

    Gerry Hammond

    Article

    Pacheco et al J Cell Biol. 2023 Feb 6;222(2):e20220409

    Insert

    Mus musculus Tub (243-505): PAmCherry1 (Tub Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEGFP-C1-ADRP

    Plasmid

    #87161

    Purpose

    Fluorescent protein localized to lipid droplets (LDs)

    Depositor

    Elina Ikonen

    Article

    Salo et al EMBO J. 2016 Dec 15;35(24):2699-2716. Ep

    Insert

    ADRP (PLIN2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1_nV5-AMOTL1

    Plasmid

    #172998

    Purpose

    constitutive expression of V5-tagged AMOTL1

    Depositor

    Michael Wehr

    Article

    Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

    Insert

    V5-AMOTL1 (AMOTL1 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. sgCtr- LentiCRISPRv2

    Plasmid

    #107402

    Purpose

    Lentiviral expression of Cas9 and a control gRNA

    Depositor

    William Kaelin

    Article

    Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

    Insert

    control gRNA
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. GFP_Rab35 Q67L active

    Plasmid

    #47425

    Depositor

    Peter McPherson

    Article

    Allaire et al J Cell Sci. 2013 Feb 1;126(Pt 3):722-31.

    Insert

    Rab35 Q67L (RAB35 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Q67L active

    Promoter

    CMV

    Available Since

    July 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. negMaLionR

    Plasmid

    #113909

    Purpose

    negative control for Red ATP sensor

    Depositor

    Tetsuya Kitaguchi

    Article

    Arai et al Angew Chem Int Ed Engl. 2018 Jun 27. doi

    Insert

    negMaLionR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG-DsRed

    Plasmid

    #11151

    Purpose

    Mammalian expression vector for expression of DsRed

    Depositor

    Connie Cepko

    Article

    Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 10

    Has Service

    Cloning Grade DNA

    Insert

    DsRed2 from Discosoma sp
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 13, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV MCS mCherry-KASH

    Plasmid

    #139654

    Purpose

    Cloning template for AAV-based ORANGE knock-in constructs

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Type

    Empty backbone

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    June 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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21. pST1374-NLS-flag-linker-Cas9

    Plasmid

    #44758

    Depositor

    Xingxu Huang

    Article

    Shen et al Cell Res. 2013 Apr 2. doi: 10.1038/cr.20

    Insert

    Human codon optimized Cas9
    

    Use

    CRISPR

    Tags

    32 amino acid linker, Flag, and NLS

    Expression

    Mammalian

    Mutation

    codon-optimized

    Promoter

    CMV

    Available Since

    April 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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22. pVITRO1-M80-F2-IgG1/κ

    Plasmid

    #50383

    Purpose

    Expresses human IgG1/κ antibody isotype with unknown specificity. Useful vector for cloning of human antibodies with kappa light chain

    Depositor

    Andrew Beavil

    Article

    Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103

    Inserts

    human Gamma 1 heavy chain expression cassette with unknown specificity
    human Kappa light chain expression cassette with unknown specificity
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Mouse Elongation Factor 1 Alpha and Rat Elongatio…

    Available Since

    Jan. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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23. HA-p73α-pcDNA3

    Plasmid

    #22102

    Depositor

    William Kaelin

    Article

    Jost et al Nature. 1997 Sep 11. 389(6647):191-4.

    Insert

    tumor protein p73 (TP73 Human)
    

    Use

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    wt cDNA

    Promoter

    Available Since

    Sept. 16, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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24. pAAV hSyn INTRON mCherry-Jph3 WPRE

    Plasmid

    #236237

    Purpose

    AAV expression of human Junctophilin3 N-terminally tagged with mCherry

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

    Insert

    Junctophilin 3 (JPH3 Human)
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    human Synapsin 1

    Available Since

    May 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pPBT/EFS-Cas9-PuroTk

    Plasmid

    #170542

    Purpose

    Expresses spCas9 with a P2A junction to the puromycin resistance gene directly fused to the thymidine kinase

    Depositor

    Jacob Giehm Mikkelsen

    Article

    Thomsen et al Mol Ther Nucleic Acids. 2022 Aug 4;29:56

    Insert

    PuroTK
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. PiggyBac-TetO-Lmx1a-t2a-Pitx3-puro

    Plasmid

    #176484

    Purpose

    TetO-Lmx1a-t2a-Pitx3 in a PiggyBac vector with puromycin resistance

    Depositor

    Marius Wernig

    Article

    Ng et al Stem Cell Reports. 2021 Jul 13;16(7):176

    Insert

    Lmx1A-T2A-Pitx3 (PITX3, LMX1A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TetO

    Available Since

    March 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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27. pINDUCER13 miR-200c-141

    Plasmid

    #81020

    Purpose

    Lentiviral vector containing doxycycline inducible human miR-200c. Puromycin selectable.

    Depositor

    Thomas Westbrook, Jeffrey Rosen

    Article

    Toneff et al BMC Biol. 2016 Jun 17;14(1):47.

    Insert

    miR-200c (MIR200C Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAAV Jph3gRNA mEmerald hSyn mTagBFP2-CAAX2

    Plasmid

    #236246

    Purpose

    Endogenous tagging of Junctophilin 3 with mEmerald with a fluorescent marker for the plasma membrane

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

    Insert

    gRNA for rat Jph3, mEmerald donor and mTagBFP2-CAAX2
    

    Use

    AAV

    Tags

    mTagBFP2

    Expression

    Mutation

    Promoter

    U6 and human Synapsin1

    Available Since

    May 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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29. pRSII416

    Plasmid

    #35456

    Depositor

    Steven Haase

    Article

    Chee et al G3 (Bethesda). 2012 May;2(5):515-26. Epu

    Type

    Empty backbone

    Use

    Yeast centromere vector

    Tags

    Expression

    Mutation

    Promoter

    None

    Available Since

    Oct. 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLVX-CMV-Galpha-oB ONE-GO

    Plasmid

    #189733

    Purpose

    GPCR/G protein BRET biosensor

    Depositor

    Mikel Garcia-Marcos

    Article

    Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

    Inserts

    Galpha-oB ONE-GO
    GNAO (Gnao1 Rat)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 19, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge