161,704 results

1. AAVS1-Neo-TRE-CMV-Cre-rtTA

   Plasmid

   #165457

   Purpose

   Tetracycline inducible CRE expression from AAVS1 locus

   Depositor

   Madeline Lancaster

   Article

   Benito-Kwiecinski et al Cell. 2021 Apr 15;184(8):2084-2102.e19.

   Insert

   CRE
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Tight TRE promoter

   Available since

   March 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pST39

   Plasmid

   #64009

   Purpose

   original polycistronic cloning vector for plasmid suite (generation I)

   Depositor

   Song Tan

   Article

   Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBWB162

   Plasmid

   #140634

   Purpose

   pBAV1k-lacI-Trc-mCherry base, lacI repression vector for ADP1

   Depositor

   Keith Tyo

   Article

   Biggs et al Nucleic Acids Res. 2020 Apr 4. pii: 5815

   Insert

   mCherry
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. phND2-N174

   Plasmid

   #31822

   Depositor

   Jerry Crabtree

   Article

   Yoo et al Nature. 2011 Jul 13. doi: 10.1038/nature

   Insert

   NeuroD2 (NEUROD2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 13, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1_MMP-9-HA

   Plasmid

   #121172

   Purpose

   Expression of mouse MMP9

   Depositor

   M. Elizabeth Fini, Shinwu Jeong

   Article

   Jeong et al Am J Pathol. 2012 May;180(5):2028-39. do

   Insert

   MMP9 (matrix metallopeptidase 9) (Mmp9 Mouse)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   See depositor comments below

   Promoter

   CMV immediate/early

   Available since

   March 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV 3xFLAG-GABARAP WT

   Plasmid

   #123097

   Purpose

   Expresses 3xFLAG-GABARAP wild-type in mammalian cells.

   Depositor

   Robin Ketteler

   Article

   Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

   Insert

   Gamma-aminobutyric acid receptor-associated protein (GABARAP Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. EGFP-Bak

   Plasmid

   #32564

   Depositor

   Richard Youle

   Article

   Nechushtan et al J Cell Biol. 2001 Jun 11. 153(6):1265-76

   Insert

   human Bak (BAK1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 29, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCas9-polyA

   Plasmid

   #72602

   Purpose

   express hCas9 byT7 promoter with polyadenine tails

   Depositor

   Tomoji Mashimo

   Article

   Yoshimi et al Nat Commun. 2016 Jan 20;7:10431. doi: 10

   Insert

   Cas9-polyadenine
   

   Use

   Tags

   81-bp polyadenylation

   Expression

   Mutation

   Promoter

   T7

   Available since

   July 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. SBE-Luc

   Plasmid

   #16527

   Depositor

   Bert Vogelstein

   Article

   Zhou et al Mol Cell. 1998 Jul . 2(1):121-7.

   Insert

   Smad binding element
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 24, 2008

   Availability

   Academic Institutions and Nonprofits only

10. pLNCX2 ER:ras

    Plasmid

    #67844

    Purpose

    4-hydroxytamoxifen inducible ER:ras fusion protein

    Depositor

    Masashi Narita

    Article

    Young et al Genes Dev. 2009 Apr 1;23(7):798-803. doi

    Insert

    ER1a H-RasG12V fusion
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    G525R renders the HBD largely insensitive to 17ß‐…

    Promoter

    CMV

    Available since

    Aug. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-EF1α-F-FLEX-mNaChBac-T2A-tdTomato

    Plasmid

    #60658

    Purpose

    Flp-dependent expression of mNaChBac-T2A-tdTomato

    Depositor

    Massimo Scanziani

    Article

    Xue et al Nature. 2014 Jul 31;511(7511):596-600. d

    Has Service

    AAV8

    Insert

    FLEX-mNaChBac-T2A-tdTomato
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    EF1α

    Available since

    Nov. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMV306hsp

    Plasmid

    #26155

    Depositor

    Siouxsie Wiles, Brian Robertson

    Article

    Andreu et al PLoS One. 2010 May 24;5(5):e10777.

    Insert

    hsp60 promoter
    

    Use

    Mycobacteria integrating vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLminP_Luc2P_RE52

    Plasmid

    #90397

    Purpose

    STAT1 - STAT1 gene reporter

    Depositor

    Ramnik Xavier

    Article

    O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

    Insert

    Firefly luciferase
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    STAT1

    Available since

    Nov. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. G5-CD40

    Plasmid

    #121166

    Purpose

    pMP71 vector expressing GFP - P2A - G5 - myc - CD40

    Depositor

    Gabriel Victora

    Article

    Pasqual et al Nature. 2018 Jan 25;553(7689):496-500. d

    Insert

    G5-CD40
    

    Use

    Retroviral

    Tags

    GFP and myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAV2-hSyn-G-Flamp1

    Plasmid

    #188569

    Purpose

    green biosensor for cAMP in neurons

    Depositor

    Jun Chu

    Article

    Wang et al Nat Commun. 2022 Sep 12;13(1):5363. doi:

    Insert

    G-Flamp1
    

    Use

    AAV

    Tags

    His tag

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    Oct. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEC3105 (pSpy1C-PxylS2_ffluc)

    Plasmid

    #218512

    Purpose

    replicative E. coli-S. pyogenes shuttle plasmid, constitutive expression of firefly luciferase reporter

    Depositor

    Emmanuelle Charpentier

    Article

    Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

    Insert

    ffluc
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    deletion of Plac_mrfp cassette

    Promoter

    PxylS2

    Available since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLVX_DSB_Spectrum-V2

    Plasmid

    #192475

    Purpose

    Lentiviral DSB Spectrum V2 Reporter System

    Depositor

    Michael Yaffe

    Article

    van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

    Insert

    BFP1.2, incompleteGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCI-ASC-HA

    Plasmid

    #41553

    Depositor

    Kate Fitzgerald

    Article

    Hornung et al Nature. 2009 Mar 26. 458(7237):514-8.

    Insert

    ASC (PYCARD Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV immediate-early enhancer/promoter

    Available since

    Dec. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDONR223-BTK

    Plasmid

    #23918

    Depositor

    David Root, William Hahn

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    BTK (BTK Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

20. pIU211

    Plasmid

    #44036

    Depositor

    Dane Wittrup

    Article

    Boder et al Nat Biotechnol. 1997 Jun;15(6):553-7.

    Insert

    AGA1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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21. Sfp pet29b C-terminal His Tag

    Plasmid

    #75015

    Purpose

    Expresses the PPTase Sfp from B. subtilis; loads CoA analogues onto ACP

    Depositor

    Michael Burkart

    Article

    Worthington et al Org Biomol Chem. 2006 Jan 7;4(1):44-6. E

    Insert

    Sfp
    

    Use

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. GFP-C1-PLCdelta-PH

    Plasmid

    #21179

    Purpose

    encodes a PI(4,5)P2 lipid selective PH domain that can be used as a fluorescent translocation biosensor to monitor changes or local differences in the concentration of plasma membrane PI(4,5)P2 lipids

    Depositor

    Tobias Meyer

    Article

    Stauffer et al Curr Biol. 1998 Mar 12. 8(6):343-6.

    Insert

    PLCdelta (PLCD1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    PH domain of human PLC delta, amino acids Met1–Il…

    Promoter

    Available since

    July 10, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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23. pCAX APP Swe/Ind

    Plasmid

    #30145

    Depositor

    Tracy Young-Pearse, Dennis Selkoe

    Article

    Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

    Insert

    human APP 695 Swedish/Indiana mutation (APP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Swedish and Indiana; K595N, M596L and V642F

    Promoter

    Available since

    Aug. 22, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pDR121

    Plasmid

    #37148

    Depositor

    Dale Ramsden

    Insert

    XRCC6 (XRCC6 Human)
    

    Use

    Tags

    H6

    Expression

    Insect

    Mutation

    Promoter

    Available since

    July 18, 2012

    Availability

    Academic Institutions and Nonprofits only

25. pLenti PGK V5-LUC Neo (w623-2)

    Plasmid

    #21471

    Purpose

    3rd gen lentiviral V5-Luciferase expression vector, PGK promoter, Neo

    Depositor

    Eric Campeau

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Insert

    V5-LUC
    

    Use

    Lentiviral and Luciferase

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 22, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pFR_HCV_xb

    Plasmid

    #11510

    Depositor

    Phil Sharp

    Article

    Petersen et al Mol Cell. 2006 Feb 17. 21(4):533-42.

    Insert

    Firefly luciferase, Hepatitis C Virus IRES sequence, Renilla, and 3'UTR short RNA binding sites
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 25, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pDR123

    Plasmid

    #37149

    Depositor

    Dale Ramsden

    Insert

    XRCC5 (XRCC5 Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    July 18, 2012

    Availability

    Academic Institutions and Nonprofits only

28. CROPseq-EFS-HypaSpCas9-P2A-puro

    Plasmid

    #106965

    Purpose

    single-vector CROPseq system with high fidelity SpCas9 (HypaSpCas9) for use in the CROPseq CRISPR knockout screen

    Depositor

    Alex Hewitt

    Insert

    HypaSpCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    April 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. FU-H2B-GFP-IRES-Puro

    Plasmid

    #69550

    Purpose

    Lentiviral, selectable fluorescent GFP tagging of nuclei.

    Depositor

    Charles Gersbach

    Insert

    Histone 2B - green fluorescent protein fusion
    

    Use

    Lentiviral

    Tags

    GFP fusion

    Expression

    Mutation

    Promoter

    Available since

    April 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLV.PGK.mFoxa2

    Plasmid

    #33014

    Depositor

    Malin Parmar

    Article

    Pfisterer et al Proc Natl Acad Sci U S A. 2011 Jun 21;10

    Insert

    Forkhead box A2 (Foxa2 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    H10L

    Promoter

    Available since

    Jan. 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge