159,238 results

1. His-Precission-NEDD4L_C942S_pCDNA3.1(+)

   Plasmid

   #187729

   Purpose

   Express the catalytically inactive NEDD4L FL in mammalian cells

   Depositor

   Sandra Gabelli

   Article

   Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

   Insert

   NEDD4L FL with C874 mutated to a serine (NEDD4L Human)
   

   Use

   Tags

   His-HRV 3C site

   Expression

   Mammalian

   Mutation

   Cys874 mutated to a serine

   Promoter

   Available since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKC52

   Plasmid

   #226780

   Purpose

   Expresses Drosophila UNC-104(R348G) in insect cells

   Depositor

   Kyoko Chiba

   Article

   Niwa et al J Cell Sci. 2024 Sep 6:jcs.262017. doi:

   Insert

   Drosophila unc-104 (R348G) (unc-104 Fly)
   

   Use

   Tags

   sfGFP and 2xStrepII

   Expression

   Insect

   Mutation

   changed Arginine 348 to Glycine

   Promoter

   Available since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKC50

   Plasmid

   #226778

   Purpose

   Expresses Drosophila UNC-104(V6M) in insect cells

   Depositor

   Kyoko Chiba

   Article

   Niwa et al J Cell Sci. 2024 Sep 6:jcs.262017. doi:

   Insert

   Drosophila unc-104 (V6M) (unc-104 Fly)
   

   Use

   Tags

   sfGFP and 2xStrepII

   Expression

   Insect

   Mutation

   changed Valine 6 to Methionine

   Promoter

   Available since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKC28

   Plasmid

   #226776

   Purpose

   Expresses Drosophila UNC-104(G618R) in insect cells

   Depositor

   Kyoko Chiba

   Article

   Niwa et al J Cell Sci. 2024 Sep 6:jcs.262017. doi:

   Insert

   Drosophila unc-104 (G618R) (unc-104 Fly)
   

   Use

   Tags

   sfGFP and 2xStrepII

   Expression

   Insect

   Mutation

   G618R

   Promoter

   Available since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDIV151

   Plasmid

   #226180

   Purpose

   Expresses Cas9 in a backbone compatible with K. phaffii.

   Depositor

   Uffe Mortensen

   Article

   Strucko et al FEMS Yeast Res. 2024 Aug 23:foae026. doi

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   SV40

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   GAP1

   Available since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. MYC-hTRAK1

   Plasmid

   #225204

   Purpose

   Expresses MYC-tagged human TRAK1

   Depositor

   Thomas Schwarz

   Article

   Pekkurnaz et al Cell. 2014 Jul 3;158(1):54-68. doi: 10.1

   Insert

   trak1 (TRAK1 Human)
   

   Use

   Tags

   MYC

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDRF1 Cyterm-ymStayGold

   Plasmid

   #219855

   Purpose

   expression of Cyterm-ymStayGoldd in budding yeast

   Depositor

   Bas Teusink

   Insert

   Cyterm-ymStayGold
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 27, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pBABE 3xHA-MKK6-EE puro

   Plasmid

   #216791

   Purpose

   Mammalian expression of constitutively active human MKK6

   Depositor

   Kevin Janes

   Insert

   MKK6 (MAP2K6 Human)
   

   Use

   Retroviral

   Tags

   3x-HA

   Expression

   Mammalian

   Mutation

   S207E and T211E, constitutively active

   Promoter

   LTR

   Available since

   May 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. MAC-C Sars-CoV-2 NSP12_P314L

   Plasmid

   #194813

   Purpose

   Sars-CoV-2 NSP12_P314L in MAC-C destination vector

   Depositor

   Markku Varjosalo

   Article

   Varjosalo et al 10 May 2024, Research Square

   Insert

   NSP12_P314L (ORF1ab Sars-CoV-2)
   

   Use

   Tags

   MAC-C

   Expression

   Mammalian

   Mutation

   P314L

   Promoter

   Available since

   May 16, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. UTS2R-DuET

    Plasmid

    #213386

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    UTS2R (UTS2R Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBiT3.1-N_TLE1:1-770

    Plasmid

    #211248

    Purpose

    Mammalian expression of full-length human TLE1. N-terminal HiBiT tag.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al bioRxiv 2024.03.03.583197

    Insert

    TLE1:M1-Y770 (TLE1 Human)
    

    Use

    Tags

    MVSGWRLFKKISGSSGGSSG

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    CMV

    Available since

    March 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. EC67843

    Plasmid

    #209513

    Purpose

    Cloning in complete guide expression cassettes, with Cas12a (D156R)

    Depositor

    Cristobal Uauy, Wendy Harwood

    Article

    Lawrenson et al Research Square, 12 March 2024, PREPRINT

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. EGFP-Rab13 in pCSDEST2

    Plasmid

    #140879

    Purpose

    Expression of zebrafish GFP-tagged Rab13 in mammalian cells.

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    Rab13 (rab13 Zebrafish)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. NDV-HN-SpyTag

    Plasmid

    #160000

    Purpose

    Expresses Newcastle disease virus HN protein (47-575) with C-terminal SpyTag fusion in mammalian cells

    Depositor

    Mark Howarth

    Article

    Rahikainen et al Angew Chem Int Ed Engl. 2020 Sep 4. doi:

    Insert

    NDV-HN-SpyTag
    

    Use

    Tags

    His6-tag

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken beta-actin promoter

    Available since

    Nov. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMSCV-puro-NKX2-8

    Plasmid

    #75080

    Purpose

    retroviral vector holding human NKX2-8 cDNA

    Depositor

    David Mu

    Article

    Kendall et al Proc Natl Acad Sci U S A. 2007 Oct 16;10

    Insert

    NKX2-8 (NKX2-8 Human)
    

    Use

    Retroviral

    Tags

    none

    Expression

    Mammalian

    Mutation

    wild-type, full-length, without native 3' UTR

    Promoter

    Available since

    May 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pENTR D-TOPO-C3_38

    Plasmid

    #60279

    Purpose

    This plasmid contains a human pancreatic islet active enhancer, which can be shuttled into a Gateway destination vector, such as pGL4.23-Gateway.

    Depositor

    Jorge Ferrer

    Article

    Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

    Insert

    ACSL1 enhancer (ACSL1 Human)
    

    Use

    Entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. lentiCRISPRv2 blast

    Plasmid

    #98293

    Purpose

    Variant of lentiCRISPRv2 that confers blasticidin S resistance

    Depositor

    Brett Stringer

    Article

    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF-1a

    Available since

    July 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-evoCAST-evoTnsC

    Plasmid

    #234730

    Purpose

    Evolved human-codon optimized PseTnsC in mammalian cells (for evoCAST)

    Depositor

    David Liu

    Article

    Witte et al Science. 2025 May 15;388(6748):eadt5199.

    Insert

    evoPseTnsC
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCMV-evoCAST-Cas6

    Plasmid

    #234725

    Purpose

    Engineered human-codon optimized PseCas6 in mammalian cells (for evoCAST)

    Depositor

    David Liu

    Article

    Witte et al Science. 2025 May 15;388(6748):eadt5199.

    Insert

    PseCas6
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCMV-evoCAST-eCas7

    Plasmid

    #234726

    Purpose

    Engineered human-codon optimized PseCas7 in mammalian cells (for evoCAST)

    Depositor

    David Liu

    Article

    Witte et al Science. 2025 May 15;388(6748):eadt5199.

    Insert

    ePseCas7
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCMV-evoCAST-eCas8

    Plasmid

    #234727

    Purpose

    Engineered human-codon optimized PseCas8 in mammalian cells (for evoCAST)

    Depositor

    David Liu

    Article

    Witte et al Science. 2025 May 15;388(6748):eadt5199.

    Insert

    ePseCas8
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCMV-evoCAST-TniQ

    Plasmid

    #234728

    Purpose

    Engineered human-codon optimized PseTniQ in mammalian cells (for evoCAST)

    Depositor

    David Liu

    Article

    Witte et al Science. 2025 May 15;388(6748):eadt5199.

    Insert

    PseTniQ
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pLJM1-EGFP

    Plasmid

    #19319

    Purpose

    3rd gen lentiviral vector for EGFP fusion; PGK driven puromycin

    Depositor

    David Sabatini

    Article

    Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pInducer20

    Plasmid

    #44012

    Purpose

    Tet-inducible lentiviral vector for ORF expression

    Depositor

    Stephen Elledge

    Article

    Meerbrey et al Proc Natl Acad Sci U S A. 2011 Mar 1;108

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA3-EGFP

    Plasmid

    #13031

    Purpose

    Mammalian expression vector for cloning your gene fused to EGFP

    Depositor

    Doug Golenbock

    Insert

    Enhanced Green Fluorescent Protein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pGFPGUSPlus

    Plasmid

    #64401

    Purpose

    binary plant vector expressing GFP and GUS, both driven by a 35S promoter

    Depositor

    Claudia Vickers

    Article

    Vickers et al Biotechnol Lett. 2007 Nov;29(11):1793-6.

    Inserts

    GFP
    GUSPlus
    

    Use

    Binary vector for plant transformation

    Tags

    His

    Expression

    Plant

    Mutation

    S65T

    Promoter

    35S

    Available since

    May 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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27. pCF159_BRL

    Plasmid

    #225962

    Purpose

    CMV-Intron-BRL (env protein). Expresses BRL (BaEVRLess) env for VLP production.

    Depositor

    Christof Fellmann

    Article

    Ramadoss et al bioRxiv [Preprint]. 2024 Jun 26:2024.06.

    Insert

    CMV-Intron-BRL (env protein)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLenti6.2_miRFP670

    Plasmid

    #113726

    Purpose

    Expresses the fluorescent protein miRFP670 in a lentiviral backbone

    Depositor

    Vanessa LaPointe

    Insert

    miRFP670
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. eSpCas9(1.1)

    Plasmid

    #71814

    Purpose

    Expresses high specificity SpCas9. Px330-like plasmid.

    Depositor

    Feng Zhang

    Article

    Slaymaker et al Science. 2015 Dec 1. pii: aad5227.

    Has Service

    Cloning Grade DNA

    Insert

    enhanced specificity Cas9 (1.1)
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    K848A, K1003A, & R1060A

    Promoter

    CBh

    Available since

    Dec. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pKDsgRNA-p15

    Plasmid

    #62656

    Purpose

    Arabinose inducible lambda red and anhydrotetracycline inducible sgRNA expression that targets pCas9 plasmid

    Depositor

    Kristala Prather

    Article

    Reisch et al Sci Rep. 2015 Oct 14;5:15096. doi: 10.10

    Insert

    exo, beta, gam, sgRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge