Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

161,805 results

Showing: 7951 - 8000 of 161805 results

  4. p4xSTAT6-Luc2P

    Plasmid
    #35554
    Depositor
    Axel Nohturfft
    Insert
    4xStat6 promoter
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    4xStat6
    Available since
    March 29, 2012
    Availability
    Academic Institutions and Nonprofits only
    35554-plasmid-map-sequence-id-338744

    Enlarge

  6. Lenti-LucS

    Plasmid
    #22778
    Depositor
    Tyler Jacks
    Insert
    SIY
    Use
    Cre/Lox and Lentiviral
    Tags
    luciferase
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 14, 2009
    Availability
    Academic Institutions and Nonprofits only
    22778-plasmid-map-sequence-id-10426

    Enlarge

  8. pSBbi-Hyg

    Plasmid
    #60524
    Purpose
    SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: hygromycin resistance gene
    Depositor
    Eric Kowarz
    Article
    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b
    Type
    Empty backbone
    Use
    Transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a/RPBSA
    Available since
    Feb. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    60524-plasmid-map-sequence-id-456411

    Enlarge

  14. pBFC0993

    Plasmid
    #231993
    Purpose
    CRISPRi-ART plasmid encoding aTc-inducible dRfxCas13d and constitutive crRNA with negative control (RFP-targeting) spacer
    Depositor
    Brady Cress
    Article
    Adler et al Nat Microbiol. 2025 Mar;10(3):694-709. d
    Inserts
    dRfxCas13d
    crRNA with negative control (RFP-targeting) spacer
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23119 and pTet
    Available since
    April 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    231993-plasmid-map-sequence-id-467616

    Enlarge

  15. pENTR-CreERT2

    Plasmid
    #139547
    Purpose
    CreERT2 ORF with stop codon between attL1-attL2 on Gateway entry vector
    Depositor
    Minoru Ko
    Insert
    CreERT2
    Use
    Cre/Lox
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    139547-plasmid-map-sequence-id-274672

    Enlarge

  17. pAAV-CMV-SpCas9D10A nickase

    Plasmid
    #216737
    Purpose
    Expresses SpCas9-D10A nickase from a CMVd1 promoter. For AAV packaging. Derived from pAAV-CMV-SpCas9 (Addgene #113034)
    Depositor
    Vincent Dion
    Article
    Alvaro et al bioRxiv
    Insert
    Cas9-D10A nickase
    Use
    AAV and CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    CMVd1
    Available since
    April 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    216737-plasmid-map-sequence-id-430337

    Enlarge

  18. VN-Tau (P301L)

    Plasmid
    #87634
    Purpose
    expresses hTau fused to the N-terminal part of Venus
    Depositor
    Tiago Outeiro
    Insert
    MAPT (MAPT Human)
    Use
    Tags
    N-terminal part of Venus
    Expression
    Mammalian
    Mutation
    Mutation P301L
    Promoter
    CMV
    Available since
    Oct. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    87634-plasmid-map-sequence-id-176933

    Enlarge

  24. pRTC2-puro

    Plasmid
    #190567
    Purpose
    Expresses the full-length human L1 (L1.3) retrotransposon and an engineered neomycin retrotransposition reporter to monitor retrotransposition efficiency in cultured mammalian cells.
    Depositor
    Anthony Furano
    Article
    Cook et al Proc Natl Acad Sci U S A. 2015 Apr 7;112
    Insert
    L1 (L1.3) with reporter cassette
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    SV40
    Available since
    June 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    190567-plasmid-map-sequence-id-393296

    Enlarge

  25. AXBEv2

    Plasmid
    #204606
    Purpose
    Mammalian expression of evolved adenine transversion base editor AXBEv2
    Depositor
    Dali Li
    Article
    Chen et al Nat Biotechnol. 2023 Jun 15. doi: 10.103
    Insert
    bpNLS-TadA-8e-nSpCas9-mAAG (R165E/Y179F)-bpNLS-P2A-GFP
    Use
    CRISPR
    Tags
    6*His
    Expression
    Mammalian
    Mutation
    mAAG (R165E/Y179F); S. pyogenes Cas9 (D10A)
    Promoter
    Available since
    Aug. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    204606-plasmid-map-sequence-id-404720

    Enlarge

  26. MADR pDonor-H3F3A-K27M-EGFP pTV1 Pdgfra D842V COTv1 Trp53-V5 WPRE

    Plasmid
    #131462
    Purpose
    MADR donor plasmid for FlpO+Cre-mediated insertion of oncogenic H3F3A K27M, PDGFRA D842V, and TRP53
    Depositor
    Joshua Breunig
    Article
    Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do
    Insert
    H3F3A-K27M-EGFP AU1 pTV1 Pdgfra D842V COTv1 Trp53-V5 WPRE
    Use
    Cre/Lox and Synthetic Biology; Mosaic analysis fo…
    Tags
    Trp53-V5, H3F3A-EGFP-AU1
    Expression
    Mammalian
    Mutation
    H3F3A K27M, Pdgfra D842V
    Promoter
    none (4x polyA to mitigate episomal expression)
    Available since
    July 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    131462-plasmid-map-sequence-id-305183

    Enlarge

  27. kiCAP-AAV-MDV1A

    Plasmid
    #196679
    Purpose
    Rep/Cap plasmid for the production of MDV1A, an AAV capsid with CNS tropism in mice.
    Depositor
    Pardis Sabeti
    Article
    Stanton et al Med (N Y). 2022 Nov 15:S2666-6340(22)004
    Insert
    AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
    Use
    AAV
    Tags
    Expression
    Mutation
    RSVGSVY insert between amino acids 588 and 589 of…
    Promoter
    p41
    Available since
    March 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    196679-plasmid-map-sequence-id-386954

    Enlarge

  29. TFORF2165

    Plasmid
    #141953
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    GRHL3 (GRHL3 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    April 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    141953-plasmid-map-sequence-id-391431

    Enlarge

  31. pSLIK-Venus

    Plasmid
    #25734
    Purpose
    Lentiviral vector for Tet-based inducible shRNA or cDNA expression, constitutive Venus fluorescent protein coexpression.
    Depositor
    Iain Fraser
    Article
    Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1
    Type
    Empty backbone
    Use
    Lentiviral and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 1, 2010
    Availability
    Academic Institutions and Nonprofits only
    25734-plasmid-map-sequence-id-12059

    Enlarge

  38. pFA6a-link-yemiRFP670-Kan

    Plasmid
    #224088
    Purpose
    Yeast tagging vector to create a gene fusion with yeast optimized emiRFP670 with Kan selection
    Depositor
    Bas Teusink
    Insert
    yemiRFP670
    Use
    Tags
    yemiRFP670
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Feb. 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    224088-plasmid-map-sequence-id-452414

    Enlarge

  42. pLentiGuide

    Plasmid
    #117986
    Purpose
    pLentiGuide is a gRNA insert lentiviral vector, with a stuffer sequence for BsmBI cloning of annealed oligos corresponding to sgRNA sequence. It has Puromycin resistance and Ampicilin resistance.
    Depositor
    Paul Khavari
    Article
    Rubin et al Cell. 2018 Dec 11. pii: S0092-8674(18)31
    Insert
    sgRNA backbone (F+E)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    117986-plasmid-map-sequence-id-235371

    Enlarge

  44. pOpen-phi29pol

    Plasmid
    #165573
    Purpose
    DNA polymerase responsible for protein-primed viral DNA replication by strand displacement with high processivity and fidelity. Possesses three enzymatic activities: DNA synthesis (polymerase), primer terminal protein (TP) deoxynucleotidylation, and 3' to 5' exonuclease activity.
    Depositor
    FreeGenes Project, Drew Endy, Jennifer Molloy
    Insert
    phi29 DNA Polymerase
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 24, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165573-plasmid-map-sequence-id-340935

    Enlarge

  45. pRK5-myc-Rac1-Q61L

    Plasmid
    #12983
    Depositor
    Gary Bokoch
    Insert
    Rac1 constitutively active (RAC1 Human)
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Q61L Constitutively active
    Promoter
    Available since
    Oct. 27, 2006
    Availability
    Academic Institutions and Nonprofits only
    12983-plasmid-map-sequence-id-154551

    Enlarge

Showing: 7951 - 8000 of 161805 results