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Showing: 8051 - 8100 of 157732 results

  1. pET28a hMDH1v3 (+TEV)

    Plasmid
    #204229
    Purpose
    Bacterial expression of human cytosolic malate dehydrogenase isoform 1 transcript variant 3 (hMDH1v3); Use in CURES (integration of research into undergraduate teaching labs).
    Depositor
    Malate dehydrogenase CUREs Community (MCC)
    Insert
    hMDH1v3 (MDH1 Human)
    Use
    Tags
    6X His
    Expression
    Bacterial
    Mutation
    Inserted a G after ATG to maintain kozak site
    Promoter
    Lac
    Available since
    Nov. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    204229-plasmid-map-sequence-id-413334

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  3. pMCB320

    Plasmid
    #89359
    Purpose
    3rd generation lentiviral sgRNA expression vector with mCherry, puromycin resistance, BlpI+BstXI cloning sites
    Depositor
    Michael Bassik
    Article
    Han et al Nat Biotechnol. 2017 Mar 20. doi: 10.103
    Insert
    puromycin resistance, mCherry
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    89359-plasmid-map-sequence-id-176457

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  4. pCC_05 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-dCas9-NLS-VPR-2A-Puro-WPRE

    Plasmid
    #139090
    Purpose
    Expresses human codon-optimized inactive SpCas9 fused to a transcriptional activator VPR in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Article
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    dSpCas9-VPR
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Promoter
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139090-plasmid-map-sequence-id-273886

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  5. pXPR_047

    Plasmid
    #107145
    Purpose
    measures Cas9 activity
    Depositor
    David Root
    Insert
    GFP + sgRNA for GFP
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 8, 2018
    Availability
    Academic Institutions and Nonprofits only
    107145-plasmid-map-sequence-id-205295

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  6. TFORF2860

    Plasmid
    #143643
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ID1 (ID1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    May 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    143643-plasmid-map-sequence-id-392313

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  12. pcDNA3.3_CoV2_501V2

    Plasmid
    #170449
    Purpose
    Express SARS-CoV-2 501V2 (South Africa strain / beta strain) spike protein with an 18aa deletion on the C-terminal tail. Used for SARS2 501V2 pseudotyped virus production.
    Depositor
    David Nemazee
    Article
    Yuan et al Science. 2021 Aug 13;373(6556):818-823.
    Insert
    SARS-CoV-2 501V2 spike D18 (S SARS-CoV-2, 501V2 (B.1.351))
    Use
    Tags
    NA
    Expression
    Mammalian
    Mutation
    L18F, D80A, D215G, R246I, K417N, E484K, N501Y, D6…
    Promoter
    CMV
    Available since
    May 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    170449-plasmid-map-sequence-id-333042

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  15. pLL3.7

    Plasmid
    #11795
    Purpose
    3rd generation lentiviral vector that expresses shRNA under the mouse U6 promoter. A CMV-EGFP reporter cassette is included in the vector to monitor expression.
    Depositor
    Luk Parijs
    Article
    Rubinson et al Nat Genet. 2003 Mar . 33(3):401-6.
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Cre/Lox, Lentiviral, and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    mouse U6
    Available since
    July 19, 2006
    Availability
    Academic Institutions and Nonprofits only
    11795-plasmid-map-sequence-id-237575

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  17. pCDNA3-hTLR4-VenusFlag

    Plasmid
    #197640
    Purpose
    Human TOLL protein expression in mammalian cells
    Depositor
    Haian Fu
    Insert
    TLR4 (TLR4 Human)
    Use
    Tags
    Venus-Flag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    197640-plasmid-map-sequence-id-391702

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  22. pComb3XTT

    Plasmid
    #63891
    Purpose
    Backbone of pComb3X containing TT Fab (light and heavy chains) against tetanus toxin, used as phage display/expression control and PCR template for human antibody constant regions (kappa and CH1)
    Depositor
    Carlos Barbas
    Article
    Andris-Widhopf et al J Immunol Methods. 2000 Aug 28;242(1-2):
    Insert
    TT Fab
    Use
    Phage display
    Tags
    6xHis, HA tag, and geneIII
    Expression
    Bacterial
    Mutation
    Promoter
    lacZ
    Available since
    July 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    63891-plasmid-map-sequence-id-450336

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  30. pKI_ΩBBMVP16&SRDXWUSm1

    Plasmid
    #220349
    Purpose
    Expresses BBM-VP16 and SRDX-WUSm1 in plant cells
    Depositor
    Tomoko Igawa
    Article
    Sato et al Front Plant Sci. 2024 Apr 3;15:1308417.
    Inserts
    BABY BOOM (BBM Mustard Weed)
    WUSCHEL (WUS Mustard Weed)
    Use
    Tags
    Superman Repression Domain X (SRDX) and VP16 tran…
    Expression
    Plant
    Mutation
    two amino acid substitution in WUS-box: L255A L25…
    Promoter
    CaMV 35S and RPS5A
    Available since
    July 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    220349-plasmid-map-sequence-id-440868

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  32. pLKO5.U6.DR130(CasRX)_GFP-targeting sgRNA.EFS.tRFP657

    Plasmid
    #212963
    Purpose
    Lentiviral plasmid for the measurement of RfxCas13d (CasRx) nuclease activity. As a marker, the plasmid also encodes the tRFP657 fluorescence protein.
    Depositor
    Roland Rad
    Article
    Montero et al Nat Methods. 2024 Feb 26. doi: 10.1038/s
    Insert
    DR1_CasRx_GFP targeting spacer
    Use
    CRISPR and Lentiviral
    Tags
    TagRFP657
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Feb. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    212963-plasmid-map-sequence-id-422499

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  34. msfGFP-Cx43

    Plasmid
    #69024
    Purpose
    Expresses rat Cx43 (Gja1 CDS) with an 8 amino acid linker on the N-terminus of the Connexin linking to msfGFP (FP fused to NT of connexin). CMV promoter.
    Depositor
    David Spray
    Article
    Stout RF et al J Biol Chem. 2015 Aug 11. pii: jbc.M115.
    Insert
    Cx43 (Gja1 Rat)
    Use
    Tags
    sfGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 23, 2015
    Availability
    Academic Institutions and Nonprofits only
    69024-plasmid-map-sequence-id-127982

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  35. pAAV-hSyn-fDIO-hM3D(Gq)-mCherry-WPREpA

    Plasmid
    #154868
    Purpose
    Human synapsin-1 promoter; Flp-dependent hM3D(Gq) DREADD-mCherry
    Depositor
    Ulrik Gether
    Has Service
    AAV Retrograde and AAV8
    Insert
    hM3D(Gq)-mCherry
    Use
    AAV; Flp-frt
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    Human Synapsin-1
    Available since
    Aug. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    154868-plasmid-map-sequence-id-308827

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  38. optoAKT

    Plasmid
    #207164
    Purpose
    Light-inducible expression of optoAKT; for precise temporal control of the AKT signaling pathway
    Depositor
    Kai Zhang
    Article
    Wang et al Elife. 2020 Oct 6;9:e57395. doi: 10.7554
    Insert
    CRY2-mCherry-AKT(_PH)-2A-CIBN2-GFP-CAAX
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    207164-plasmid-map-sequence-id-413072

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  39. pcDNA3-ps-CFP

    Plasmid
    #13034
    Purpose
    Expresses photo-switchable CFP in mammalian cells. Can undergo irreversible photoconversion from cyan to green fluorescence
    Depositor
    Doug Golenbock
    Insert
    photo-switchable cyan fluorescent protein
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 2, 2013
    Availability
    Academic Institutions and Nonprofits only
    13034-plasmid-map-sequence-id-7430

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  42. TFORF0058

    Plasmid
    #143007
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    MAF (MAF Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    143007-plasmid-map-sequence-id-408960

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  44. pORTMAGE-4

    Plasmid
    #72679
    Purpose
    Expresses Lambda Red recombinases and a dominant negative MutL allele all controlled by temperature sensitve cI857 repressor for high precision and efficiency MAGE experiments. Cam resistance marker.
    Depositor
    Csaba Pál
    Article
    Nyerges et al Proc Natl Acad Sci U S A. 2016 Feb 16. p
    Insert
    mutL E32K
    Use
    Tags
    none
    Expression
    Bacterial
    Mutation
    E32K mutation conferring dominant mutator phenoty…
    Promoter
    Available since
    Feb. 18, 2016
    Availability
    Academic Institutions and Nonprofits only
    72679-plasmid-map-sequence-id-198908

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Showing: 8051 - 8100 of 157732 results