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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 19,527 results for: ACE;

Showing: 6481 - 6520 of 19527 results

  1. pCDNA3-EMTB-TurboID-V5

    Plasmid
    #190736
    Purpose
    Mammalian expression of EMTB fusion to V5-tagged TurboID on microtubules (EMTB as the microtubule-targeting tag)
    Depositor
    Jeremy Baskin
    Article
    Cao et al ACS Chem Biol. 2022 Aug 11. doi: 10.1021
    Insert
    ensconsin (MAP7 Human)
    Use
    Tags
    Myc, TurboID, and V5
    Expression
    Mammalian
    Mutation
    Microtubule binding-domain of ensconsin (amino ac…
    Promoter
    CMV
    Available since
    Dec. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    190736-plasmid-map-sequence-id-379320

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  3. qTAG-N-Solo-mStayGold-TUBA1B

    Plasmid
    #227327
    Purpose
    Donor template for mStayGold insertion into the N-terminus of the TUBA1B locus. For tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B (Addgene #207763)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    TUBA1B Homology Arms flanking a mStayGold Tag (TUBA1B Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Promoterless
    Available since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227327-plasmid-map-sequence-id-452117

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  4. pSB1A3 EcoRI-RTX with EcoRI Methylase-AmilCP

    Plasmid
    #85165
    Purpose
    Provides the coding region to express the EcoRI restriction enzyme. The RTX tag allows the EcoRI enzyme to be isolated via calcium-precipitation.
    Depositor
    Robin Dowell
    Article
    Hendrix et al ACS Synth Biol. 2014 Dec 19;3(12):969-71
    Insert
    EcoRI-RTX and EcoRI Methylase-AmilCP coding sequences
    Use
    Tags
    AmilCP and RTX
    Expression
    Bacterial
    Mutation
    Promoter
    J23100 promoter
    Available since
    Dec. 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    85165-plasmid-map-sequence-id-354765

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  5. qTAG-C-mStayGold-Puro-H2BC11

    Plasmid
    #227332
    Purpose
    Donor template for mStayGold-2A-Puro insertion into the C-terminus of the H2BC11 locus. For nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 (Addgene #207755)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    H2BC11 Homology Arms flanking a mStayGold-2A-Puro Cassette (H2BC11 Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Promoterless
    Available since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227332-plasmid-map-sequence-id-452132

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  14. pAP05

    Plasmid
    #186261
    Purpose
    sfGFP under light light responsive PEL222 promoter and EL222 under constitutively active BBa_J2305 promoter
    Depositor
    Yongku Cho
    Article
    Pirhanov et al ACS Synth Biol. 2021 Feb 19;10(2):345-35
    Inserts
    sfGFP
    EL222 (ELI_04755 Erythrobacter litoralis)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    BBa_23105 (GGCTAGCTCAGTCCTAGGTACTATGCTAGC) and PE…
    Available since
    Sept. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    186261-plasmid-map-sequence-id-369315

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  15. qTAG-C-moxGFP-Puro-H2BC11

    Plasmid
    #207758
    Purpose
    Donor template for moxGFP-2A-Puro insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    H2BC11 Homology Arms flanking a moxGFP-Puro Cassette (H2BC11 Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 17, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    207758-plasmid-map-sequence-id-415316

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  22. pBBR1.cas9-NT

    Plasmid
    #197871
    Purpose
    Constitutive expression of Cas9 endonuclease, trancRNA and crRNA with a randomly generated spacer sequence (non-targeting)
    Depositor
    Baojun Wang
    Article
    Huan et al ACS Synth Biol. 2023 Mar 17;12(3):709-72
    Inserts
    trancCRNA
    Cas9
    crRNA (non-chromosomal (E. coli/S. flexneri)-targeting spacer sequence)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    197871-plasmid-map-sequence-id-396456

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  26. pCK306

    Plasmid
    #110544
    Purpose
    rhaBAD, a rhamnose-inducible promoter, YFP, an E. coli-Synechocystis shuttle vector, chromosomal-integration sites. Allows precise & sustained gene expression in cyanobacteria.
    Depositor
    John Heap
    Article
    Kelly et al ACS Synth Biol. 2018 Apr 2. doi: 10.1021
    Inserts
    PrhaBAD
    yfp
    rhaS
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    N/A, PrhaBAD, and kanR promoter (upstream of kanR…
    Available since
    June 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    110544-plasmid-map-sequence-id-212164

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  27. CMV-R-GECO1.2

    Plasmid
    #45494
    Purpose
    Red intensiometric genetically encoded Ca2+-indicators for optical imaging 1.2
    Depositor
    Robert Campbell
    Article
    Wu et al ACS Chem Neurosci. 2013 Mar 19.
    Insert
    R-GECO1.2
    Use
    Tags
    Expression
    Mammalian
    Mutation
    R-GECO1 M164R/I166V/V174L/F222L/N267S/S270T/I330M…
    Promoter
    CMV
    Available since
    July 23, 2013
    Availability
    Academic Institutions and Nonprofits only
    45494-plasmid-map-sequence-id-123029

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Showing: 6481 - 6520 of 19527 results