We narrowed to 14,621 results for: RING
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Plasmid#176231PurposeA template plasmid for amplification of the cI-hok-neo cassette, which contains a truncated H207 promoter of T5 phage. The promoter is directed outward the cI gene.DepositorInsertcI of phage lambda, hok of the R1 plasmid, neo, truncated PH207 promoter of T5 phage
UseSynthetic BiologyExpressionBacterialAvailable SinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTmpKmA1O4in
Plasmid#176232PurposeA template plasmid for amplification of the cI-hok-neo cassette, which contains A1O4 promoter. The promoter is directed toward the cI gene.DepositorInsertcI of phage lambda, hok of the R1 plasmid, neo, hybrid PA1O4 promoter
UseSynthetic BiologyExpressionBacterialAvailable SinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTmpKmA1O4out
Plasmid#176233PurposeA template plasmid for amplification of the cI-hok-neo cassette, which contains LacI-repressible A1O4 promoter. The promoter is directed outward the cI gene.DepositorInsertcI of phage lambda, hok of the R1 plasmid, neo, hybrid PA1O4 promoter
UseSynthetic BiologyExpressionBacterialAvailable SinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSws1-nfsB-CFP
Plasmid#86375Purposefor zebrafish transgenesis, expression of nfsB-CFP, also expresses EGFPDepositorAvailable SinceFeb. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
DNA-CARtcr-IRESpuro
Plasmid#89346Purpose2nd generation lentiviral vector which expresses SNAP tagged TCRbeta subunit upstream of a IRESpuro cassetteDepositorInsertTCR beta gene (Jurkat allele) fused at the N terminus to the SNAP tag
UseLentiviralTagsSNAPf tagExpressionMammalianPromoterSFFVAvailable SinceJune 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
optoPKAW196R/Y204A
Plasmid#104546PurposeExpresses Cry2-mCherry-PKA W196R/Y204A in mammalian cells.DepositorInsertCry2-mCherry-PKA W196R/Y204A
TagsmCherryExpressionMammalianMutationPKA W196R and Y204APromoterCMVAvailable SinceMarch 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBTK599
Plasmid#110600PurposeBTK Type 8 Bacterial origin (R6K suicide plasmid) for homologous recombinationDepositorInsertGFP dropout
UseSynthetic BiologyAvailable SinceFeb. 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pPI304
Plasmid#113232Purposeextrachromosomal expression vector, expression of LifeAct-mCherry/PH-pkgE-GFPDepositorInsertLifeAct & pkgE-PH
UseDictyostelium discoideum expression vector, extra…TagsmCherry & GFPPromoteract15Available SinceAug. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRS303 Gal TDP-43 YFP
Plasmid#27468DepositorAvailable SinceFeb. 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMBA333
Plasmid#214745PurposeMedium copy thyA-infA plasmid containing gfp and no antibiotic resistance gene (ARG)DepositorInsertBBa_J23119-RiboJ-RBS(21992)-gfpmut3
ExpressionBacterialAvailable SinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBTK564
Plasmid#110613PurposeBTK assembled broad-host-range plasmid encoding NanoLuc luciferase protein driven by PA1 promoterDepositorInsertNanoluc
ExpressionBacterialAvailable SinceAug. 10, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUb dRxCas13d + RxCas13d guide RNA
Plasmid#176304PurposeExpresses catalytic dead RxCas13d and its associated guide RNA in Drosophila cellsDepositorInsertRxCas13d
TagsHA tagExpressionInsectMutationR239A, H244A, R858A, H863APromoterUbi-p63eAvailable SinceMarch 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
YFP-DAXX-mSIM1
Plasmid#134524PurposeExpresses YFP-DAXX fusion protein with a mutation in its 5' SIMDepositorInsertDAXX-mSIM1
TagsYFPExpressionMammalianMutationSIM (IIVL) 7-10 aa changed to IGAGPromoterCMVAvailable SinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 CARD8
Plasmid#169962PurposepDONR221 for Gateway shuttling. Contains the CARD8 T60 isoform (no stop codon)DepositorInsertCARD8 (CARD8 Human)
UseGateway cloning vectorAvailable SinceJuly 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMDJ21 - pEXP [Psmu-1 | eFlp recombinase (PATCs) | smu-1 UTR]
Plasmid#159810PurposeOptimized enzyme for genome editing in C. elegansDepositorInsertpEXP [Psmu-1 | eFlp recombinase (PATCs) | smu-1 UTR]
ExpressionWormMutationNot applicableAvailable SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
(424) pcDNA3.1(+)-HA-LaG6-(EAAAK)4-OGT(4.5, K852A)
Plasmid#168191PurposeHA-tagged GFP nanobody Lag6 (310 nM affinity) fused to an inactive truncated OGT of 4 TPRs with K852A mutationDepositorInsertHA-nLag6-(EAAAK)4-OGT(4,K852A)
TagsHA-TagExpressionMammalianPromoterT7/CMVAvailable SinceMay 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
DNA-CARzeta-IRESpuro
Plasmid#89345Purpose2nd generation lentiviral vector which expresses DNA-CAR receptor upstream of a IRES-puro cassetteDepositorInsertDNA-CARzeta
UseLentiviralTagsSNAPf tagExpressionMammalianAvailable SinceJune 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV/3' Box_(GLuc)_INT
Plasmid#68436PurposeTransient expression of an "INT" construct_bearing three PP7 Stem-loops, targeting the GLuc reporter, in mammalian cells. CMV/3' Box expression backbone.DepositorInsertINT construct_bearing three PP7 Stem-loops
UseCRISPR and Synthetic BiologyExpressionMammalianPromoterCMVAvailable SinceSept. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBabe-PI(WT)-Src(YF)-mCherry
Plasmid#87357PurposePI(WT): Photo-inhibitable/blue light sensitive; YF: Y535F const. active mutationDepositorInsertsTagsmCherryExpressionBacterial and MammalianMutationY535FPromoterCMVAvailable SinceApril 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAB088h
Plasmid#79215PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPlacZ-opt (OR1+2) gIII, luxAB; Ppro1 434cI-TnCAD-F3
ExpressionBacterialPromoterPlacZ-opt (OR1+2); Ppro1Available SinceDec. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDIV300
Plasmid#204968PurposeAMA1 plasmid with Aspergillus optimized Mad7 and hph (hygromycin) resistance markerDepositorInsertsMad7
hph (hygromycin resistance marker)
UseCRISPR; Fungal expressionTagsSV40 NLSExpressionBacterialPromoterAspergillus nidulans tef1 promoter and Aspergillu…Available SinceNov. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUWR-CadTS-C
Plasmid#58326PurposeDestination vector for inserting ubi-CadTS control to Drosophila melanogaster genomeDepositorInsertE-cadherin tension sensor control
ExpressionInsectPromoterpoly-ubiquitinAvailable SinceAug. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGLucIPZ
Plasmid#228703PurposeConstitutive expression of Gaussia luciferase, puromycin N-acetyl-transferase and shRNAmir of interestDepositorInsertsGaussia luciferase
non-silencing shRNA
UseLentiviralExpressionMammalianAvailable SinceDec. 2, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
asPink chromoprotein promoter-less
Plasmid#118005PurposeFor course in "Synthetic Biology: A Lab Manual"DepositorInsertRBS, asPink
UseEscherichia coliMutationBioBrick sites removedAvailable SinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCVL Traffic Light Reporter 1.1 (Ani target) Ef1a Puro
Plasmid#31480DepositorInsertTraffic Light Reporter 1.1 (ani target)
UseLentiviralMutationmCherry has M9S, M16L to reduce background mCherr…Available SinceAug. 22, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCyterm-mScarlet-I3-NCwt_N1
Plasmid#189779PurposeConstruct for testing oligimerization of mScarlet-I3-NCwt in mammalian cells by OSER assayDepositorInsertCyterm-mScarlet-I3-NCwt
ExpressionMammalianPromoterCMVAvailable SinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMSP1D1R1
Plasmid#97323PurposeNanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include an additional R residue shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.DepositorInsertMSP1D1R1
Tags6xHis - TEVExpressionBacterialMutationAddition of R residue after T25PromoterT7Available SinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSimpleII-U6-tracr-U6-crRNA(tdTomato)-NLS-NmCas9-HA-NLS(s)
Plasmid#47869PurposeAll-in-one plasmid targeting tdTomatoDepositorInsertsNmCas9
U6pr-tracrRNA
tdTomato crRNA
UseCRISPRTagsHA and NLSExpressionMammalianAvailable SinceSept. 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
NAX_CAGGS_CAS9_m4_VPR_mcherry
Plasmid#167886PurposePiggyBac compatible plasmid expressing dCas9-VPRDepositorInsertdCas9-VPR
UseCRISPRMutationD10A, D839A, H840A, N863AAvailable SinceSept. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-YPet-WPRE
Plasmid#193342PurposeAAV-mediated expression of YPet under the TRE promoter in mammalian cellsDepositorInsertYPet
UseAAVExpressionMammalianPromoterTREAvailable SinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
TRE-UBC-mCD59-T2A-GFP
Plasmid#205451Purposelentiviral plasmid for expression of mouse CD59DepositorAvailable SinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
Ensconsin-18-mEos2
Plasmid#99230PurposeMammalian expression of the photoconvertible protein mEos2 as an Ensconsin fusion.DepositorInsertmEos2
TagsEnsconsin (MAP7)ExpressionMammalianPromoterCMVAvailable SinceOct. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTC394
Plasmid#91224Purposeprotoplast vector expressing gRNA24 targeting tomato ANT1 (control without TREX2 expression)DepositorInsertgRNA targeting tomato ANT1
UseCRISPRExpressionPlantAvailable SinceJuly 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJL1-MBP-4xDQNAT
Plasmid#128390PurposeIn vitro expression of E. coli MBP with C terminal 4xDQNAT glycosylation sitesDepositorInsertMaltose binding protein
Tags4xDQNAT glycosylation tag and 6xHis tagExpressionBacterialMutationA302T- please see depositor commentPromoterT7Available SinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pARF1-YFP
Plasmid#11390DepositorAvailable SinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
pMOD_B2615
Plasmid#91078PurposeModule B, Promoter: AtU6, Gene: BsaI ccdb cassette for single gRNA spacer cloning, Terminator: Pol IIIDepositorInsertBsaI ccdb cassette for single gRNA spacer cloning
UseCRISPRPromoterAtU6Available SinceJuly 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLPP5
Plasmid#209967PurposeContains Level 0 Part: inducible Promoter (PsppQ) with two regulatory genes sppK and sppR for the construction of Level 1 plasmidsDepositorInsertPromoter (P_sppQ)
ExpressionBacterialAvailable SinceSept. 13, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV-TRE-mRuby3-WPRE
Plasmid#193343PurposeAAV-mediated expression of mRuby3 under the TRE promoter in mammalian cellsDepositorInsertmRuby3
UseAAVExpressionMammalianPromoterTREAvailable SinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSF-pMB1'-t7- YFP
Plasmid#186564PurposepMB1' ori, lacI PT7 promoter, AmpR. Kringle YFP (Yellow Fluorescence Protein) expressionDepositorInsertYFP
ExpressionBacterialPromoterT7 promoterAvailable SinceNov. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
p168
Plasmid#62618PurposeePathBrick pETM6 vector encoding CsF3H, FaDFR, and DuLARDepositorInsertsflavaone 3B-hydroxylase
dihydroflavonol 4-reductase
leucoanthocyanidin reductase
UseSynthetic BiologyTagsGBD, PDZ, and SH3ExpressionBacterialPromoterT7Available SinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
rAAV-CAG::FLEX-rev:ChR2HA:2a:PSAML141F,Y115F:GlyR
Plasmid#32483PurposeCre-dependent expression of ChR2 and PSAM-GlyR (L141F,Y115F) neuronal inhibitor, with IRES-EGFP markerDepositorInsertChR2HA-2a-PSAML141F,Y115F:GlyR
UseAAV and Cre/Lox; Adeno-associated virus, flex swi…TagsChR2 2APromoterCAGAvailable SinceApril 11, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTC392
Plasmid#91223Purposeprotoplast vector expressing gRNA23 targeting tomato ANT1 (control without TREX2 expression)DepositorInsertgRNA targeting tomato ANT1
UseCRISPRExpressionPlantAvailable SinceJuly 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBTK509
Plasmid#110610PurposeBTK assembled broad-host-range plasmid encoding GFP driven by PA2 promoterDepositorInsertGFP
ExpressionBacterialAvailable SinceAug. 21, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBTK615
Plasmid#110609PurposeBTK assembled plasmid - used in Stage 2 assembly to target Cas9 or dCas9DepositorInsertsgRNA
UseSynthetic BiologyAvailable SinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
TB:7xSMAD_RE:MiniP
Plasmid#133879Purpose7 copies the SMAD DNA binding motifDepositorInsert7 copies the SMAD DNA binding motif
UseLuciferase and Synthetic BiologyExpressionMammalianAvailable SinceJune 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSR02
Plasmid#69149PurposeRead-out loxP mCherry to GFP switch for integration on CxTi10816, Mos chr IVDepositorInsertsmCherry
eGFP
UseCre/Lox; MossciExpressionWormMutationcodon-optimzed index 1.0Promoterrps-27Available SinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBLO2 casX
Plasmid#123122PurposeE. coli expression vector for CasX sgRNADepositorInsertCasX guide RNA
UseCRISPRAvailable SinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBTK121
Plasmid#110581PurposeBTK Type 2 promoter for golden gate assemblyDepositorInsertPA3 Promoter with RBS
UseSynthetic BiologyAvailable SinceFeb. 20, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSin-5HT-1.0
Plasmid#172882Purposemake sindbis virus of 5-HT sensor GRAB_5-HT1.0 and express in cellsDepositorInsert5HT SENSOR
ExpressionMammalianPromotersp6Available SinceOct. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A0402
Plasmid#91009PurposeModule A, Promoter: AtUbi10, Gene: AtCas9_dead (D10A + H840A), Terminator: HSPDepositorInsertAtCas9_dead (D10A + H840A)
UseCRISPRMutationD10A, H840APromoterAtUbi10Available SinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only