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We narrowed to 8,515 results for: SET

Showing: 8201 - 8250 of 8515 results

  1. pLKO_HA.FLAG.VSVg_NGFR

    Plasmid
    #158245
    Purpose
    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
    Depositor
    Brian Brown
    Article
    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d
    Insert
    Pro-Code Tagged human dNGFR (NGFR Human)
    Use
    CRISPR and Lentiviral
    Tags
    HA.FLAG.VSVg
    Expression
    Mutation
    Truncation of the signaling domain
    Promoter
    EF1a
    Available since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    158245-plasmid-map-sequence-id-327706

    Enlarge

  2. pMIA3

    Plasmid
    #109399
    Purpose
    Expresses sgRNA cassette (BsmBI cloning site) and EF1-A driven eSpCas9 linked via P2A with mRuby2 and dominant negative mtp53 for enhanced survival of hES after DSB
    Depositor
    Roger Foo
    Article
    Ang et al Cell Rep. 2018 Feb 20;22(8):2190-2205. d
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    mRuby2 and mtp53dn
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    109399-plasmid-map-sequence-id-211963

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  3. pLKO_NWS.FLAG.VSVg_NGFR

    Plasmid
    #158233
    Purpose
    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
    Depositor
    Brian Brown
    Article
    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d
    Insert
    Pro-Code Tagged human dNGFR (NGFR Human)
    Use
    CRISPR and Lentiviral
    Tags
    NWS.FLAG.VSVg
    Expression
    Mutation
    Truncation of the signaling domain
    Promoter
    EF1a
    Available since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    158233-plasmid-map-sequence-id-327650

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  4. pMDC32B-AtTAS1c-D2-B/c-AtMIR173

    Plasmid
    #137885
    Purpose
    Plant expression vector for direct cloning of synthetic trans-acting siRNAs into Arabidopsis thaliana TAS1c precursor downstream 3'D1[+]. Contains AtMIR173 for syntasi expression in any plant species.
    Depositor
    Alberto Carbonell
    Article
    Lopez-Dolz et al Nucleic Acids Res. 2020 May 12. pii: 583
    Inserts
    AtTAS1c-D2-B/c
    2x35S-AtMIR173-Tnos
    Use
    Tags
    Expression
    Plant
    Mutation
    A. thaliana TAS1c precursor sequence including a …
    Promoter
    Available since
    May 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    137885-plasmid-map-sequence-id-275856

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  5. pKK-RNAi-nucCHERRYmiR-EGFP-TEV

    Plasmid
    #105810
    Purpose
    Functional studies using RNAi; rescue experiments; substituting protein for its different form. miRNA cassette with nuclear mCherry expression marker; your protein of interest with N-terminal EGFP.
    Depositor
    Andrzej Dziembowski
    Article
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    RNAi; Flp-in competent
    Tags
    EGFP-TEV
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    105810-plasmid-map-sequence-id-204854

    Enlarge

  6. pKK-RNAi-nucCHERRYmiR-FLAG-TEV

    Plasmid
    #105809
    Purpose
    Functional studies using RNAi; rescue experiments; substituting protein for its different form. miRNA cassette with nuclear mCherry expression marker; your protein of interest with N-terminal FLAG.
    Depositor
    Andrzej Dziembowski
    Article
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    RNAi; Flp-in competent
    Tags
    FLAG-TEV
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    105809-plasmid-map-sequence-id-203432

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  7. TFORF1778

    Plasmid
    #142851
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    WHSC1 (NSD2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    May 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    142851-plasmid-map-sequence-id-392726

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  9. pPL001

    Plasmid
    #184750
    Purpose
    Big-IN positive control payload encoding EF1a-driven GFP-T2A-BSD.
    Depositor
    Jef Boeke
    Article
    Brosh et al Proc Natl Acad Sci U S A. 2021 Mar 9;118
    Insert
    pEF1a-eGFP-T2A-BSD-bGHpA
    Use
    Cre/Lox, Mouse Targeting, and Synthetic Biology ;…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    184750-plasmid-map-sequence-id-365374

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  10. pEN313 - Rad21-Halo-Frt-PGK-EM7-NeoR-bpA-Frt targeting

    Plasmid
    #156431
    Purpose
    Targeting vector to introduce an Halotag cassette at the mouse Rad21 locus using NEOMYCINE selection. Designed for using with sgRNA CCACGGTTCCATATTATCTG
    Depositor
    Elphege Nora
    Article
    Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:
    Insert
    RAD21, Halotag (Rad21 Mouse)
    Use
    Mouse Targeting
    Tags
    Expression
    Mammalian
    Mutation
    none
    Promoter
    Available since
    Sept. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    156431-plasmid-map-sequence-id-310238

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  11. pAAV-hSyn-DIO {hCAR}off-{DTR-GFP}on-W3SL

    Plasmid
    #111396
    Purpose
    AAV vector with hSynapsin promoter, Cre-OFF hCAR (for efficient CAV-2 infection), Cre-ON DTR-EGFP (diphtheria toxin receptor for cell ablation), and W3SL cassette (for maximize cloning capacity)
    Depositor
    Adam Kepecs
    Article
    Li et al Neuron. 2018 Jun 6;98(5):905-917.e5. doi
    Inserts
    hCAR (CXADR Human)
    DTR-EGFP
    Use
    AAV
    Tags
    EGFP and Myc
    Expression
    Mammalian
    Mutation
    Promoter
    hSynapsin
    Available since
    June 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    111396-plasmid-map-sequence-id-212262

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  12. pLKO_S.NWS.VSVg_NGFR

    Plasmid
    #158348
    Purpose
    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
    Depositor
    Brian Brown
    Article
    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d
    Insert
    Pro-Code Tagged human dNGFR (NGFR Human)
    Use
    CRISPR and Lentiviral
    Tags
    S.NWS.VSVg
    Expression
    Mutation
    Truncation of the signaling domain
    Promoter
    EF1a
    Available since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    158348-plasmid-map-sequence-id-330894

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  13. ssAAV-EF1a-FLuc-WPRE-HgHpA_bac_293

    Plasmid
    #118412
    Purpose
    Expresses firefly luciferase under the control of a strong ubiquitous promoter EF1a along with a WPRE cassette. Backbone is compatible with both baculoviral and human production methods of AAV.
    Depositor
    Nicole Paulk
    Article
    Rumachik et al Mol Ther Methods Clin Dev. 2020 May 22;1
    Insert
    Firefly luciferase
    Use
    AAV, Luciferase, and Synthetic Biology
    Tags
    Expression
    Insect and Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Nov. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    118412-plasmid-map-sequence-id-231800

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  14. pCC_12 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-KRAB-dxCas9NG-NLS-2A-Puro-WPRE

    Plasmid
    #139097
    Purpose
    Expresses human codon-optimized inactive xCas9-NG fused to a transcriptional repressor KRAB in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Article
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    KRAB-dxCas9-NG
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    D10A, H840A, A262T,R324L, S409I, E480K, E543D, M6…
    Promoter
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139097-plasmid-map-sequence-id-273896

    Enlarge

  15. pLKO_FLAG.Ollas.VSVg_NGFR

    Plasmid
    #158324
    Purpose
    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
    Depositor
    Brian Brown
    Article
    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d
    Insert
    Pro-Code Tagged human dNGFR (NGFR Human)
    Use
    CRISPR and Lentiviral
    Tags
    FLAG.Ollas.VSVg
    Expression
    Mutation
    Truncation of the signaling domain
    Promoter
    EF1a
    Available since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    158324-plasmid-map-sequence-id-330232

    Enlarge

  17. pKK-RNAi-nucEGFPmiR-TEV-FLAG

    Plasmid
    #105811
    Purpose
    Functional studies using RNAi; rescue experiments; substituting protein for its different form. miRNA cassette with nuclear EGFP expression marker; your protein of interest with C-terminal FLAG.
    Depositor
    Andrzej Dziembowski
    Article
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    RNAi; Flp-in competent
    Tags
    TEV-FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    105811-plasmid-map-sequence-id-204856

    Enlarge

  18. pLKO_S.FLAG.VSVg_NGFR

    Plasmid
    #158345
    Purpose
    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
    Depositor
    Brian Brown
    Article
    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d
    Insert
    Pro-Code Tagged human dNGFR (NGFR Human)
    Use
    CRISPR and Lentiviral
    Tags
    S.FLAG.VSVg
    Expression
    Mutation
    Truncation of the signaling domain
    Promoter
    EF1a
    Available since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    158345-plasmid-map-sequence-id-330661

    Enlarge

  20. pLKO_HSV.FLAG.HA_NGFR

    Plasmid
    #158294
    Purpose
    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
    Depositor
    Brian Brown
    Article
    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d
    Insert
    Pro-Code Tagged human dNGFR (NGFR Human)
    Use
    CRISPR and Lentiviral
    Tags
    HSV.FLAG.HA
    Expression
    Mutation
    Truncation of the signaling domain
    Promoter
    EF1a
    Available since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    158294-plasmid-map-sequence-id-329340

    Enlarge

  21. pLKO_HA.NWS.V5_NGFR

    Plasmid
    #158248
    Purpose
    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
    Depositor
    Brian Brown
    Article
    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d
    Insert
    Pro-Code Tagged human dNGFR (NGFR Human)
    Use
    CRISPR and Lentiviral
    Tags
    HA.NWS.V5
    Expression
    Mutation
    Truncation of the signaling domain
    Promoter
    EF1a
    Available since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    158248-plasmid-map-sequence-id-327729

    Enlarge

  22. pLKO_HA.NWS.FLAG_NGFR

    Plasmid
    #158249
    Purpose
    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
    Depositor
    Brian Brown
    Article
    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d
    Insert
    Pro-Code Tagged human dNGFR (NGFR Human)
    Use
    CRISPR and Lentiviral
    Tags
    HA.NWS.FLAG
    Expression
    Mutation
    Truncation of the signaling domain
    Promoter
    EF1a
    Available since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    158249-plasmid-map-sequence-id-329483

    Enlarge

  24. pKK-RNAi-nucEGFPmiR-mCherry-TEV

    Plasmid
    #105808
    Purpose
    Functional studies using RNAi; rescue experiments; substituting protein for its different form. miRNA cassette with nuclear EGFP expression marker; your protein of interest with N-terminal mCherry.
    Depositor
    Andrzej Dziembowski
    Article
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    RNAi; Flp-in competent
    Tags
    mCherry-TEV
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    105808-plasmid-map-sequence-id-274623

    Enlarge

  25. pKK-RNAi-nucEGFPmiR-FLAG-TEV

    Plasmid
    #105807
    Purpose
    Functional studies using RNAi; rescue experiments; substituting protein for its different form. miRNA cassette with nuclear EGFP expression marker; your protein of interest with N-terminal FLAG tag.
    Depositor
    Andrzej Dziembowski
    Article
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    RNAi; Flp-in competent
    Tags
    FLAG-TEV
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    105807-plasmid-map-sequence-id-203410

    Enlarge

  27. pLVX-CMV-XRCC1-gRNA res-Neo

    Plasmid
    #176149
    Purpose
    XRCC1 with dual PAM resistance to XRCC1 gRNA1 and XRCC1 gRNA2 & a neomycin/G418 resistance cassette
    Depositor
    Robert Sobol
    Article
    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:
    Insert
    X-ray repair cross complementing 1 (XRCC1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Dual PAM resistance to XRCC1 gRNA 1 and XRCC1 gRN…
    Promoter
    CMV
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    176149-plasmid-map-sequence-id-353190

    Enlarge

  28. pLKO_S.C.V5_NGFR

    Plasmid
    #158331
    Purpose
    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
    Depositor
    Brian Brown
    Article
    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d
    Insert
    Pro-Code Tagged human dNGFR (NGFR Human)
    Use
    CRISPR and Lentiviral
    Tags
    S.C.V5
    Expression
    Mutation
    Truncation of the signaling domain
    Promoter
    EF1a
    Available since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    158331-plasmid-map-sequence-id-330479

    Enlarge

  29. pCC_07 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-dxCas9-NLS-VPR-2A-Puro-WPRE

    Plasmid
    #139092
    Purpose
    Expresses human codon-optimized inactive xCas9 3.7 fused to a transcriptional activator VPR in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Article
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    dxCas9 3.7-VPR
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    D10A, H840A, A262T, R324L,S409I, E480K, E543D, M6…
    Promoter
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139092-plasmid-map-sequence-id-273889

    Enlarge

  30. pLKO_HSV.Ollas.FLAG_NGFR

    Plasmid
    #158270
    Purpose
    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
    Depositor
    Brian Brown
    Article
    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d
    Insert
    Pro-Code Tagged human dNGFR (NGFR Human)
    Use
    CRISPR and Lentiviral
    Tags
    HSV.Ollas.FLAG
    Expression
    Mutation
    Truncation of the signaling domain
    Promoter
    EF1a
    Available since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    158270-plasmid-map-sequence-id-331329

    Enlarge

  31. pKK-RNAi-nucCHERRYmiR-TEV-FLAG

    Plasmid
    #105813
    Purpose
    Functional studies using RNAi; rescue experiments; substituting protein for its different form. miRNA cassette with nuclear mCherry expression marker; your protein of interest with C-terminal FLAG.
    Depositor
    Andrzej Dziembowski
    Article
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    RNAi; Flp-in competent
    Tags
    TEV-FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    105813-plasmid-map-sequence-id-204858

    Enlarge

  32. pLKO_Ollas.C.VSVg_NGFR

    Plasmid
    #158315
    Purpose
    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
    Depositor
    Brian Brown
    Article
    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d
    Insert
    Pro-Code Tagged human dNGFR (NGFR Human)
    Use
    CRISPR and Lentiviral
    Tags
    Ollas.C.VSVg
    Expression
    Mutation
    Truncation of the signaling domain
    Promoter
    EF1a
    Available since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    158315-plasmid-map-sequence-id-329898

    Enlarge

  33. pLKO_Tag.C.S.Ollas_NGFR

    Plasmid
    #158303
    Purpose
    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
    Depositor
    Brian Brown
    Article
    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d
    Insert
    Pro-Code Tagged human dNGFR (NGFR Human)
    Use
    CRISPR and Lentiviral
    Tags
    Tag.C.S.Ollas
    Expression
    Mutation
    Truncation of the signaling domain
    Promoter
    EF1a
    Available since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    158303-plasmid-map-sequence-id-329631

    Enlarge

  34. pLKO_HSV.Ollas.C_NGFR

    Plasmid
    #158268
    Purpose
    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
    Depositor
    Brian Brown
    Article
    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d
    Insert
    Pro-Code Tagged human dNGFR (NGFR Human)
    Use
    CRISPR and Lentiviral
    Tags
    HSV.Ollas.C
    Expression
    Mutation
    Truncation of the signaling domain
    Promoter
    EF1a
    Available since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    158268-plasmid-map-sequence-id-328074

    Enlarge

  35. pLKO_Ollas.C.V5_NGFR

    Plasmid
    #158310
    Purpose
    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
    Depositor
    Brian Brown
    Article
    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d
    Insert
    Pro-Code Tagged human dNGFR (NGFR Human)
    Use
    CRISPR and Lentiviral
    Tags
    Ollas.C.V5
    Expression
    Mutation
    Truncation of the signaling domain
    Promoter
    EF1a
    Available since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    158310-plasmid-map-sequence-id-329893

    Enlarge

  36. pLKO_Ollas.C.FLAG_NGFR

    Plasmid
    #158311
    Purpose
    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
    Depositor
    Brian Brown
    Article
    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d
    Insert
    Pro-Code Tagged human dNGFR (NGFR Human)
    Use
    CRISPR and Lentiviral
    Tags
    Ollas.C.FLAG
    Expression
    Mutation
    Truncation of the signaling domain
    Promoter
    EF1a
    Available since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    158311-plasmid-map-sequence-id-329894

    Enlarge

  37. pLKO_Ollas.C.NWS_NGFR

    Plasmid
    #158312
    Purpose
    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
    Depositor
    Brian Brown
    Article
    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d
    Insert
    Pro-Code Tagged human dNGFR (NGFR Human)
    Use
    CRISPR and Lentiviral
    Tags
    Ollas.C.NWS
    Expression
    Mutation
    Truncation of the signaling domain
    Promoter
    EF1a
    Available since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    158312-plasmid-map-sequence-id-329895

    Enlarge

  38. pLKO_Ollas.C.HA_NGFR

    Plasmid
    #158313
    Purpose
    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
    Depositor
    Brian Brown
    Article
    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d
    Insert
    Pro-Code Tagged human dNGFR (NGFR Human)
    Use
    CRISPR and Lentiviral
    Tags
    Ollas.C.HA
    Expression
    Mutation
    Truncation of the signaling domain
    Promoter
    EF1a
    Available since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    158313-plasmid-map-sequence-id-329896

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  39. pLKO_HA.VSVg.C_NGFR

    Plasmid
    #158263
    Purpose
    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
    Depositor
    Brian Brown
    Article
    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d
    Insert
    Pro-Code Tagged human dNGFR (NGFR Human)
    Use
    CRISPR and Lentiviral
    Tags
    HA.VSVg.C
    Expression
    Mutation
    Truncation of the signaling domain
    Promoter
    EF1a
    Available since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    158263-plasmid-map-sequence-id-328059

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  40. pLKO_FLAG.VSVg.C_NGFR

    Plasmid
    #158260
    Purpose
    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
    Depositor
    Brian Brown
    Article
    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d
    Insert
    Pro-Code Tagged human dNGFR (NGFR Human)
    Use
    CRISPR and Lentiviral
    Tags
    FLAG.VSVg.C
    Expression
    Mutation
    Truncation of the signaling domain
    Promoter
    EF1a
    Available since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    158260-plasmid-map-sequence-id-327912

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  41. pKK-RNAi-nucCHERRYmiR-TEV-EGFP

    Plasmid
    #105814
    Purpose
    Functional studies using RNAi; rescue experiments; substituting protein for its different form. miRNA cassette with nuclear mCherry expression marker; your protein of interest with C-terminal EGFP.
    Depositor
    Andrzej Dziembowski
    Article
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    RNAi; Flp-in competent
    Tags
    TEV-EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    105814-plasmid-map-sequence-id-203426

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  42. pCDF-casBCDE(+6)

    Plasmid
    #89728
    Purpose
    Expresses the Cascade subunits Cse2, Cas7, Cas5, Cas6e and pre-crRNA containing a longer version of the wt spacer (+6) extended by 6 nucleotides at the leader-distal end.
    Depositor
    Konstantin Severinov, Konstantin Kuznedelov
    Article
    Kuznedelov et al Nucleic Acids Res. 2016 Dec 15;44(22):10
    Insert
    CRISPR array
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Derivative CRISPR array containing a longer versi…
    Promoter
    Available since
    May 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    89728-plasmid-map-sequence-id-177602

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  44. MKC84_HBB(HBA1reg-HBA1)

    Plasmid
    #232410
    Purpose
    AAV production plasmid for HBA1 UTRs vector from Fig. 3 that mediates HDR at HBB locus using HBB sg7 gRNA. HBA gene includes HBA1 exons and introns; HBA1 UTRs flank HBA1 cassette.
    Depositor
    Kyle Cromer
    Article
    Chu et al bioRxiv [Preprint]. 2024 May 7:2023.09.0
    Insert
    Hemoglobin Subunit Alpha 1 (HBA1 Human)
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    232410-plasmid-map-sequence-id-463061

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  45. MKC83_HBB(HBBreg-HBA1)

    Plasmid
    #232409
    Purpose
    AAV production plasmid for HBB UTRs vector from Fig. 3 that mediates HDR at HBB locus using HBB sg7 gRNA. HBA gene includes HBA1 exons and introns; HBB UTRs flank HBA1 cassette.
    Depositor
    Kyle Cromer
    Article
    Chu et al bioRxiv [Preprint]. 2024 May 7:2023.09.0
    Insert
    Hemoglobin Subunit Alpha 1 (HBA1 Human)
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    232409-plasmid-map-sequence-id-465033

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  46. Lenti-SAM-Puro

    Plasmid
    #198713
    Purpose
    For expression of guide RNAs and the SAM transcriptional activator for CRISPRa in mammalian cells.
    Depositor
    Tyler Jacks
    Article
    Gaglia et al Cancer Cell. 2023 May 8;41(5):871-886.e1
    Inserts
    U6-MS2-gRNAscaffold
    EF1a-MPH-Puro
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a and U6
    Available since
    Jan. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    198713-plasmid-map-sequence-id-402626

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  47. Lenti-SAM-Cre

    Plasmid
    #198714
    Purpose
    For expression of guide RNAs, the SAM transcriptional activator for CRISPRa and Cre in mammalian cells
    Depositor
    Tyler Jacks
    Article
    Gaglia et al Cancer Cell. 2023 May 8;41(5):871-886.e1
    Inserts
    U6-MS2-gRNAscaffold
    Pgk-MPH-Cre
    Use
    Cre/Lox and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Pgk and U6
    Available since
    Jan. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    198714-plasmid-map-sequence-id-402628

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  49. ku70 insUP+ pXYL-G418-tNOS-pADH2-crtE-tNOS-pXYL-crtI-tNOS-pGPD-crtYB-tNOS+ku70 insD (SBE146)

    Plasmid
    #195048
    Purpose
    cassette for the deletion of ku70 and overexpression of crtE, crtI, and crtYB under different combination of promoters
    Depositor
    Petri-Jaan Lahtvee
    Article
    Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.
    Inserts
    crtE
    crtI
    crtYB
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    pADH2, pGPD, and pXYL
    Available since
    Nov. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    195048-plasmid-map-sequence-id-386137

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  50. ku70 insUP+ pXYL-G418-tNOS-pADH2-crtE-tNOS-pGPD-crtI-tNOS-pXYL-crtYB-tNOS+ku70 insD (SBE145)

    Plasmid
    #195047
    Purpose
    cassette for the deletion of ku70 and overexpression of crtE, crtI, and crtYB under different combination of promoters
    Depositor
    Petri-Jaan Lahtvee
    Article
    Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.
    Inserts
    crtE
    crtI
    crtYB
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    pADH2, pGPD, and pXYL
    Available since
    Nov. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    195047-plasmid-map-sequence-id-386131

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Showing: 8201 - 8250 of 8515 results