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178,651 results

Showing: 481 - 510 of 178651 results

  2. Inducible Caspex expression

    Plasmid
    #97421
    Purpose
    For stable clone generation of cells expressing the dCas9-APEX2 fusion protein for Genomic locus proteomics
    Depositor
    Steven Carr, Samuel Myers
    Publication
    Myers et al Nat Methods. 2018 May 7. pii: 10.1038/s4
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Caspex
    T2A-Gfp
    Use
    CRISPR
    Tags
    3xFLAG and V5
    Expression
    Mammalian
    Mutation
    dCas9: D10A and H840A, APEX2: K14D, W41F, E112K, …
    Promoter
    TRE3G
    Available since
    Oct. 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    97421-plasmid-map-sequence-id-466895

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  6. pHIV-Luc-ZsGreen

    Plasmid
    #39196
    Depositor
    Bryan Welm
    Inserts
    Firefly Luciferase Luc2P
    ZsGreen
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1-alpha and EF1-alpha
    Available since
    Oct. 30, 2012
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    39196-plasmid-map-sequence-id-335121

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  13. pcDNA3.1/hChR2(H134R)-mCherry

    Plasmid
    #20938
    Purpose
    Mammalian expression of humanized ChR2 with H134R mutation fused to mCherry for optogenetic activation
    Depositor
    Karl Deisseroth
    Publication
    Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    channelrhodopsin-2
    Expression
    Mammalian
    Mutation
    H134R mutation in ChR2
    Promoter
    CMV
    Available since
    June 1, 2009
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    20938-plasmid-map-sequence-id-181022

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  16. lenti sgRNA(MS2)_puro backbone

    Plasmid
    #73795
    Purpose
    3rd generation lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Feng Zhang
    Publication
    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6 and EF1A
    Available since
    March 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    73795-plasmid-map-sequence-id-181202

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  26. pCMV-PEmax-P2A-GFP

    Plasmid
    #180020
    Purpose
    Mammalian expression of SpCas9 PEmax prime editor with P2A-EGFP marker
    Depositor
    David Liu
    Publication
    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PEmax-P2A-EGFP
    Tags
    P2A-EGFP, SV40 bpNLS, and c-Myc NLS
    Expression
    Mammalian
    Mutation
    Detailed in manuscript
    Promoter
    CMV
    Available since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    180020-plasmid-map-sequence-id-352752

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Showing: 481 - 510 of 178651 results