165,688 results

1. pN1-PinkyCaMP

   Plasmid

   #232861

   Purpose

   A vector expressing the mScarlet based calcium indicator PinkyCaMP under CMV promoter

   Depositor

   Olivia Masseck

   Article

   Fink et al bioRxiv [Preprint]. 2025 Jan 11:2024.12.

   Insert

   PinkyCaMP
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. Lenti-Cas9-gRNA-GFP

   Plasmid

   #124770

   Purpose

   3rd generation lentiviral plasmid encoding Cas9, GFP, and a gRNA backbone

   Depositor

   Jason Sheltzer

   Article

   Giuliano et al Curr Protoc Mol Biol. 2019 Sep;128(1):e1

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Oct. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. nlsSalicS1

   Plasmid

   #232190

   Purpose

   Novel FRET-based biosensor for salicylic acid

   Depositor

   Alexander Jones

   Article

   Tang et al Science. 2025 Oct 9;390(6769):188-194. d

   Insert

   nlsSalicS1
   

   Tags

   His-tag

   Expression

   Plant

   Promoter

   p16

   Available Since

   Oct. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. H2B-GFP

   Plasmid

   #11680

   Depositor

   Geoff Wahl

   Article

   Kanda et al Curr Biol. 1998 Mar 26. 8(7):377-85.

   Insert

   Human histone H2B (H2BC11 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   May 31, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. HyPer7.2DAAO-NES

   Plasmid

   #168301

   Purpose

   Chemogenetic fluorescent H2O2 reporter targeted to the cytoplasm

   Depositor

   Thomas Michel

   Article

   Saeedi Saravi et al Redox Biol. 2020 Sep;36:101605. doi: 10.

   Insert

   HyPer7.2DAAO-NES
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHAGE-mt-mKeima

   Plasmid

   #131626

   Purpose

   lentiviral expression of mitochondrial mKeima for generation of stable cell lines

   Depositor

   Richard Youle

   Article

   Vargas et al Mol Cell. 2019 Apr 18;74(2):347-362.e6.

   Insert

   mt-mKeima
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. Cas9 sgRNA vector

   Plasmid

   #68463

   Purpose

   Cas9 sgRNA vector for cloning

   Depositor

   Su-Chun Zhang

   Article

   Chen et al Cell Stem Cell. 2015 Jul 1. pii: S1934-5

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Nov. 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAR015 Lenti pEF-dEnAsCas12a-NLS-NFZ-3XHA-T2A-BSD

   Plasmid

   #195544

   Purpose

   dCas12a effector fusion for manipulating transcription

   Depositor

   Michael Bassik

   Article

   Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

   Insert

   Lenti pEF-dEnAsCas12a-NLS-NFZ-3XHA-T2A-BSD
   

   Use

   CRISPR and Lentiviral

   Tags

   3XHA

   Expression

   Mammalian

   Promoter

   pEF1a

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUCmini-iCAP-PHP.N

   Plasmid

   #127851

   Purpose

   non-standard AAV2 rep-AAV-PHP.N cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

   Depositor

   Viviana Gradinaru

   Article

   Ravindra Kumar et al Nat Methods. 2020 May;17(5):541-550. doi

   Insert

   Synthetic construct isolate AAV-PHP.N VP1 gene
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   p41

   Available Since

   April 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. EF1a-TET1-dCas9

    Plasmid

    #235593

    Purpose

    Tet1CD fused to N-terminus of dCas9

    Depositor

    Ting Zhou

    Article

    Wu et al Stem Cell Reports. 2025 Apr 25:102499. d

    Insert

    TET1-dCas9 (TET1 Human, SpCas9 is from Streptococcus pyogenes)
    

    Use

    CRISPR

    Tags

    3xNLS, TET1 CD, and tagBFP

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    May 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. mNeonGreen-mRuby2-FRET-10

    Plasmid

    #58179

    Purpose

    Localization: Cytosolic FRET, Excitation: , Emission:

    Depositor

    Michael Davidson

    Article

    Shaner et al Nat Methods. 2013 May;10(5):407-9. doi:

    Type

    Empty backbone

    Tags

    mNeonGreen and mRuby2

    Expression

    Mammalian

    Available Since

    March 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. Mito-SNAP

    Plasmid

    #206480

    Purpose

    Encodes a mitochondrially-targeted SNAP tag for mammalian cell expression

    Depositor

    Erika Holzbaur

    Article

    Harding et al Mol Cell. 2023 Sep 7;83(17):3188-3204.e7

    Insert

    Mitochondrial targeting signal from COX8A fused to SNAP (derived from human MGMT)
    

    Tags

    SNAPf

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET-NT*-HRV3CP

    Plasmid

    #162795

    Purpose

    Expressess an optimized construct of human rhinovirus 14 3C protease (NT*-HRV3CP)

    Depositor

    Gottfried Otting

    Article

    Abdelkader et al J Biotechnol. 2020 Nov 6. pii: S0168-165

    Insert

    Human rhinovirus 14 3C protease
    

    Tags

    NT* solubility tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. HyPer7.2DAAO-mito

    Plasmid

    #168304

    Purpose

    Chemogenetic fluorescent H2O2 reporter targeted to the mitochondria

    Depositor

    Thomas Michel

    Article

    Saeedi Saravi et al Redox Biol. 2020 Sep;36:101605. doi: 10.

    Insert

    HyPer7.2DAAO-mito
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pYPQ153

    Plasmid

    #69304

    Purpose

    Gateway entry vector with pco-dCas9-3X(SRDX)

    Depositor

    Yiping Qi

    Article

    Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

    Insert

    pco-dCas9-3X(SRDX) (Plant codon-optimized)
    

    Use

    CRISPR

    Tags

    2XFLAG

    Mutation

    SRDX (X3) repressor fusion; NLS fusion

    Available Since

    Oct. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAWP89

    Plasmid

    #61264

    Purpose

    IncP-based replicating vector expressing dTomato under tac promoter.

    Depositor

    Mary Lidstrom

    Article

    Puri et al Appl Environ Microbiol. 2014 Dec 29. pii

    Insert

    Ptac-dTomato
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    Feb. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. circRNA-synIRES-R25-mNeonGreen

    Plasmid

    #188115

    Purpose

    Template plasmid to transcribe circular RNA encoding mNeonGreen

    Depositor

    Howard Chang

    Article

    Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

    Insert

    mNeonGreen
    

    Expression

    Mammalian

    Promoter

    T7

    Available Since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLV-TRE3G-YAP1-5SA-CMV-mCherry-T2A-Puro

    Plasmid

    #176851

    Purpose

    lentiviral vector for doxycycline-inducible YAP1 5SA constitutively active expression

    Depositor

    Roland Friedel

    Article

    Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

    Insert

    hYAP1-5SA (YAP1 Human)
    

    Use

    Lentiviral

    Mutation

    S61A, S109A, S127A, S164A, S381A (phosphorylation…

    Available Since

    Nov. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV-evoCAST-evoTnsAB

    Plasmid

    #234729

    Purpose

    Evolved human-codon optimized PseTnsAB in mammalian cells (for evoCAST)

    Depositor

    David Liu

    Article

    Witte et al Science. 2025 May 15;388(6748):eadt5199.

    Insert

    evoPseTnsAB
    

    Expression

    Mammalian

    Available Since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. HyPer7.2DAAO-NLS

    Plasmid

    #168302

    Purpose

    Chemogenetic fluorescent H2O2 reporter targeted to the nucleus

    Depositor

    Thomas Michel

    Article

    Saeedi Saravi et al Redox Biol. 2020 Sep;36:101605. doi: 10.

    Insert

    HyPer7.2DAAO-NLS
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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21. pLJM1-Tmem192-mRFP-3xHA

    Plasmid

    #134631

    Purpose

    bait for affinity-isolation of lysosomes

    Depositor

    Roberto Zoncu

    Article

    Lim et al Nat Cell Biol. 2019 Oct;21(10):1206-1218

    Insert

    TMEM192 (TMEM192 Human)
    

    Use

    Lentiviral

    Tags

    3xHA, TEV, and mRFP

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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22. pCAH01

    Plasmid

    #128162

    Purpose

    IncP-based broad host range vector used for inducible expression of genes in methanotrophic bacteria

    Depositor

    Michael Guarnieri, Calvin Henard

    Article

    Henard et al Sci Rep. 2016 Feb 23;6:21585. doi: 10.10

    Type

    Empty backbone

    Expression

    Bacterial

    Promoter

    Ptet

    Available Since

    July 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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23. pLKO.1 hygro

    Plasmid

    #24150

    Purpose

    3rd gen lentiviral backbone for cloning and expression of new shRNA sequences. Uses hygromycin for selection.

    Depositor

    Bob Weinberg

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    U6 for shRNA

    Available Since

    March 15, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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24. pRBC-sfGFP-150TAG

    Plasmid

    #174076

    Purpose

    Plasmid with p15a origin of replication expressing codon optimized superfolder GFP-150 TAG with C-terminal His6 tag, under T7 promoter,

    Depositor

    Ryan Mehl

    Article

    Zhu et al ACS Chem Biol. 2019 Jul 19;14(7):1564-15

    Insert

    Superfolder GFP
    

    Tags

    His-6

    Expression

    Bacterial

    Mutation

    N150TAG

    Promoter

    T7

    Available Since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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25. pCasSA

    Plasmid

    #98211

    Purpose

    CRISPR-based E. coli/Staphylococcus aureus temperature-sensitive plasmid for genome editing in Staphylococcus aureus using S. pyogenes Cas9

    Depositor

    Quanjiang Ji

    Article

    Chen et al J Am Chem Soc. 2017 Mar 15;139(10):3790-

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Available Since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCMV-evoCAST-evoTnsC

    Plasmid

    #234730

    Purpose

    Evolved human-codon optimized PseTnsC in mammalian cells (for evoCAST)

    Depositor

    David Liu

    Article

    Witte et al Science. 2025 May 15;388(6748):eadt5199.

    Insert

    evoPseTnsC
    

    Expression

    Mammalian

    Available Since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAAV2/6

    Plasmid

    #240485

    Purpose

    AAV packaging plasmid expressing AAV2 Rep protein and AAV6 capsid protein

    Depositor

    Addgene Research Program, Meghan Rego

    Insert

    AAV2 Rep and AAV6 Cap
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    July 25, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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28. pAAVS1-P-CAG-mCh

    Plasmid

    #80492

    Purpose

    donor vector for AAVS1 targeting (puromycin selection) and constitutive mCherry expression

    Depositor

    Knut Woltjen

    Article

    Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

    Insert

    mCherry
    

    Use

    Donor vector (human)

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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29. lentiMPH v2

    Plasmid

    #89308

    Purpose

    lenti vector encoding the MS2-P65-HSF1 activator helper complex with a 2A Hygro resistance marker (EF1a-MS2-p65-HSF1-2A-Hygro-WPRE). This version has a higher virus titer.

    Depositor

    Feng Zhang

    Article

    Joung et al Nat Protoc. 2017 Apr;12(4):828-863. doi:

    Insert

    MS2-P65-HSF1_2A_Hygro (RELA Human, Synthetic)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    N55K in MS2

    Promoter

    EF1a

    Available Since

    March 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCMV-evoCAST-TniQ

    Plasmid

    #234728

    Purpose

    Engineered human-codon optimized PseTniQ in mammalian cells (for evoCAST)

    Depositor

    David Liu

    Article

    Witte et al Science. 2025 May 15;388(6748):eadt5199.

    Insert

    PseTniQ
    

    Expression

    Mammalian

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge