We narrowed to 17,931 results for: COMP

1. pFRT_TO_FlagHA_G3BP1

   Plasmid

   #127106

   Depositor

   Thomas Tuschl

   Article

   Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

   Insert

   G3BP1 (G3BP1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. AAV-hSYN-eGFP

   Plasmid

   #162512

   Purpose

   AAV vector plasmid expressing enhanced green fluorescent protein (eGFP) under the human synapsin (hSYN) promoter

   Depositor

   Bart Nieuwenhuis

   Article

   Nieuwenhuis et al Gene Ther. 2020 Jun 23. pii: 10.1038/s41

   Insert

   eGFP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hSYN

   Available since

   April 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMIA4.721-Pansio

   Plasmid

   #214441

   Purpose

   BxbI landing-pad HDR donor for targeting Pansio-1 GSH. CAGG-driven Casp9-P2A-Bsd.

   Depositor

   Roger Foo

   Article

   Autio et al Elife. 2024 Jan 2;13:e79592. doi: 10.755

   Insert

   2xFKBP12-Casp9
   

   Use

   Hdr donor

   Tags

   P2A-Bsd

   Expression

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pENTR4_G3BP1

   Plasmid

   #127104

   Depositor

   Thomas Tuschl

   Article

   Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

   Insert

   G3BP1 (G3BP1 Human)
   

   Use

   Gateway shuttling vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pDGB3alpha2_35S:P19:Tnos (GB1203)

   Plasmid

   #68214

   Purpose

   Transcriptional unit for Tomato bushy stunt virus (TBSV) silencing suppressor P19 plant expression driven by the 35S promoter

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   P19 silencing suppressor
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   35S

   Available since

   March 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pEntry_HCoV-OC43-Spike

   Plasmid

   #168951

   Purpose

   Gateway-compatible Entry vector

   Depositor

   Pascal Falter-Braun

   Article

   Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

   Insert

   HCoV-OC43-Spike (S )
   

   Use

   Gateway-compatible entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. hRAD18-EGFP

   Plasmid

   #68824

   Purpose

   Expresses human RAD18 tagged with EGFP

   Depositor

   Satoshi Tateishi

   Article

   Tsuji et al Genes Cells. 2008 Apr;13(4):343-54. doi:

   Insert

   human RAD18 tagged with EGFP (RAD18 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pEntry_HCoV-HKU1-Spike

   Plasmid

   #168911

   Purpose

   Gateway-compatible Entry vector

   Depositor

   Pascal Falter-Braun

   Article

   Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

   Insert

   HCoV-HKU1-Spike (S )
   

   Use

   Gateway-compatible entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pU6-sgRNA-CBh-eGFP

   Plasmid

   #206885

   Purpose

   cloning backbone for sgRNA using OptScf2

   Depositor

   Jacob Giehm Mikkelsen

   Article

   Haldrup et al Nucleic Acids Res. 2023 Sep 8:gkad676. d

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Oct. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. HA-Vps34

    Plasmid

    #86749

    Purpose

    N-terminal HA-tagged protein expression in mammalian cells

    Depositor

    Do-Hyung Kim

    Article

    Park et al Autophagy. 2016;12(3):547-64. doi: 10.10

    Insert

    human Vps34 (PIK3C3 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. mEGFP-Rac1

    Plasmid

    #83950

    Purpose

    Rac1 FLIM donor for dynamic imaging of GTPase activity

    Depositor

    Ryohei Yasuda

    Article

    Hedrick et al Nature. 2016 Sep 28;538(7623):104-108. d

    Insert

    ras-related C3 botulinum toxin substrate 1 (RAC1 Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Lenti-FAB(mito-LD)

    Plasmid

    #214421

    Purpose

    Detection of mito-lipid droplet contact site

    Depositor

    Chi-Lun Chang

    Article

    Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

    Insert

    RspAN-3xHA-V5-mito_IRES_CFAST-20nm-Halo-6xHp
    

    Use

    Lentiviral

    Tags

    CFAST-Halo and RspAN-3XHA-V5

    Expression

    Mutation

    Promoter

    CMV

    Available since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. HA-Beclin 1

    Plasmid

    #86748

    Purpose

    N-terminal HA-tagged protein expression in mammalian cells

    Depositor

    Do-Hyung Kim

    Article

    Park et al Autophagy. 2016;12(3):547-64. doi: 10.10

    Insert

    human Beclin 1 (BECN1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pEGFP/golgin-160

    Plasmid

    #180630

    Purpose

    Expression of WT golgin-160

    Depositor

    Carolyn Machamer

    Article

    Mancini et al J Cell Biol. 2000 May 1;149(3):603-12. d

    Insert

    golgin-160 (GOLGA3 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET28a-MH6-RspCas13d_RspCasWYL1

    Plasmid

    #108304

    Purpose

    Expresses E. coli codon optimized RspCas13d and RspCasWYL1 in the pET28a backbone.

    Depositor

    Arbor Biotechnologies

    Article

    Yan et al Mol Cell. 2018 Mar 9. pii: S1097-2765(18

    Inserts

    RspCas13d
    RspWYL1
    

    Use

    CRISPR

    Tags

    mH6 tag

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEGFP PICH (Nigg CB62)

    Plasmid

    #41163

    Depositor

    Erich Nigg

    Article

    Baumann et al Cell. 2007 Jan 12;128(1):101-14.

    Insert

    PICH (ERCC6L Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3 IKKE K38A flag

    Plasmid

    #26202

    Depositor

    Tom Maniatis

    Article

    Fitzgerald et al Nat Immunol. 2003 May;4(5):491-6.

    Insert

    I-kappa-B kinase epsilon K38A (IKBKE Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Catalytic Lysine changed to Alanine (K38A)

    Promoter

    Available since

    Aug. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCRISPR-cBEST-v2-PermE

    Plasmid

    #209446

    Purpose

    improved pCRISPR-cBEST plasmid with optimized sgRNA cloning site

    Depositor

    Tilmann Weber

    Article

    Maleckis et al ACS Chem Biol. 2024 Feb 10. doi: 10.1021

    Insert

    Codon optimized APOBEC1-nCas9-UGI fusion protein
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    PtipA; sgRNA expression from ermE* promoter

    Available since

    March 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJMP3

    Plasmid

    #79875

    Purpose

    Bacillus subtilis sgRNA expression vector; integrates into thrC

    Depositor

    Stanley Qi, Carol Gross, Jason Peters

    Article

    Peters et al Cell. 2016 Jun 2;165(6):1493-506. doi: 1

    Insert

    sgRNA RR1
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    veg

    Available since

    Sept. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PAmCherry1-C1-LACT-C2

    Plasmid

    #202682

    Purpose

    Photoactivatable lipid biosensor

    Depositor

    Gerry Hammond

    Article

    Pacheco et al J Cell Biol. 2023 Feb 6;222(2):e20220409

    Insert

    PAmCherry1: Bos taurus MFGE8 (274-431) (MFGE8 Bovine)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pcDNA5/FRT/TO V5 HSPA1A

    Plasmid

    #19510

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    heat shock 70kDa protein 1A (HSPA1A Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 7, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. T7-Elongin C-pcDNA3

    Plasmid

    #19998

    Depositor

    William Kaelin

    Article

    Lonergan et al Mol Cell Biol. 1998 Feb . 18(2):732-41.

    Insert

    Elongin C (ELOC Human)
    

    Use

    Tags

    T7-tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 6, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pEGFP-C1-BBS2

    Plasmid

    #218712

    Purpose

    Expresses N-terminally EGFP-tagged BBS2 in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Katoh et al J Cell Sci. 2015 May 11. pii: jcs.168740

    Insert

    BBS2 (BBS2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pHH0103_TP53BP2-1/1

    Plasmid

    #91503

    Purpose

    Protein expression and purification of human SH3 domain construct TP53BP2-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    TP53BP2-1/1 (TP53BP2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET28-His-TEV-NQO1

    Plasmid

    #119058

    Purpose

    For expression in E.coli and affinity purification of N-terminally His tagged NQO1

    Depositor

    Yosef Shaul

    Article

    Tsvetkov et al Proc Natl Acad Sci U S A. 2005 Apr 12;10

    Insert

    NAD(P)H dehydrogenase, quinone 1 (NQO1) (NQO1 Human)
    

    Use

    Tags

    6His, TEV site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. dxCas(3.7)-VPR

    Plasmid

    #108383

    Purpose

    Mammalian expression vector for xCas9 3.7 with VP64-p65-Rta (VPR) fused to its C-terminus

    Depositor

    David Liu

    Article

    Hu et al Nature. 2018 Feb 28. pii: nature26155. d

    Insert

    dxCas(3.7)-VPR
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    A262T, R324L, S409I, E480K, E543D, M694I, E1219V

    Promoter

    Available since

    March 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAAV.pU1a-SpCas9

    Plasmid

    #121507

    Purpose

    Expresses codon-optimized SpCas9 in mammalian cells.

    Depositor

    Guangping Gao

    Article

    Wang et al Nat Biotechnol. 2018 Oct;36(9):839-842.

    Insert

    SpCas9
    

    Use

    AAV

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    U1a

    Available since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pB[vasa-Cas9 PUb-ZsGreen]

    Plasmid

    #169012

    Purpose

    For germline transformation. Expresses Cas9 in germ cells and ZsGreen marker in all cells.

    Depositor

    Max Scott

    Article

    Kandul et al CRISPR J. 2021 Oct;4(5):739-751. doi: 10

    Insert

    Cas9
    

    Use

    Piggybac

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Drosophila melanogaster vasa

    Available since

    April 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. Lenti-FAB(ER-mito)

    Plasmid

    #214423

    Purpose

    Detection of ER-mito contact site

    Depositor

    Chi-Lun Chang

    Article

    Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

    Insert

    RspAN-FRB-V5-Sec61_IRES_CFAST10-20nm-Halo-mito
    

    Use

    Lentiviral

    Tags

    CFAST10-Halo and RspAN-FRB-V5

    Expression

    Mutation

    Promoter

    CMV

    Available since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pC001 - huLshC2C2-MBP for bacterial expression

    Plasmid

    #79150

    Purpose

    Expresses human codon-optimized LshC2c2 for purification in E. coli

    Depositor

    Feng Zhang

    Article

    Abudayyeh et al Science. 2016 Jun 2. pii: aaf5573.

    Insert

    C2c2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pZac2.1 gfaABC1D-Aqp4-BioID2-BioID2-HA

    Plasmid

    #176742

    Purpose

    Expresses Aquaporin-4 fused with BioID2 at the C-terminus in astrocytes

    Depositor

    Baljit Khakh

    Article

    Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

    Insert

    Aqp4-BioID2-BioID2-HA
    

    Use

    AAV

    Tags

    HA

    Expression

    Bacterial

    Mutation

    Promoter

    gfaABC1D

    Available since

    April 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pSmart-Kan-HC-EF1a-Nla-Cas8-7-5-11

    Plasmid

    #193752

    Purpose

    human codon optimized Nla-cas8c-Cas7c-Cas5c-Cas11c cloned into pSmart-HC-Kan-EF1a-bGH

    Depositor

    Yan Zhang

    Article

    Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

    Insert

    EF1a-NlaCas8c-Cas7c-Cas5c-Cas11c-bGH PolyA
    

    Use

    Tags

    HA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pT7-EGFP-C1-HsEDC3

    Plasmid

    #25032

    Depositor

    Elisa Izaurralde

    Article

    Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

    Insert

    EDC3 (EDC3 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 22, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCIneo-V5-SBP-HseIF4AI_AC

    Plasmid

    #148438

    Purpose

    Mammalian Expression of HseIF4AI

    Depositor

    Elisa Izaurralde

    Article

    Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

    Insert

    HseIF4AI (EIF4A1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    3 silent mutations relative to NM_001416.2

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pKLV2-EF1a-Cas9GFP-W

    Plasmid

    #200100

    Purpose

    Lentiviral vector expressing Cas9 fused with GFP at the C-terminus

    Depositor

    Kosuke Yusa

    Article

    Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

    Insert

    GFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pX330-EN479

    Plasmid

    #86234

    Purpose

    For transient expression of spCas9-nuclease and a sgRNA targeting the mouse Rosa26 locus

    Depositor

    Benoit Bruneau

    Article

    Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

    Insert

    spCas9-nickase and sgRNA against mouse rosa26 locus
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pJMP2

    Plasmid

    #79874

    Purpose

    Bacillus subtilis sgRNA expression vector; integrates into amyE

    Depositor

    Stanley Qi, Carol Gross, Jason Peters

    Article

    Peters et al Cell. 2016 Jun 2;165(6):1493-506. doi: 1

    Insert

    sgRNA RR1
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    veg

    Available since

    Sept. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pRK5 FLAG wildtype, catalytic active lipin 1

    Plasmid

    #32005

    Depositor

    David Sabatini

    Article

    Peterson et al Cell. 2011 Aug 5;146(3):408-20.

    Insert

    lipin1 (Lpin1 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, SP6

    Available since

    Sept. 13, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. HA WDR24 pRK5

    Plasmid

    #46335

    Depositor

    David Sabatini

    Article

    Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

    Insert

    WDR24 (WDR24 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pEntry_HCoV-229E-Spike

    Plasmid

    #168971

    Purpose

    Gateway-compatible Entry vector

    Depositor

    Pascal Falter-Braun

    Article

    Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

    Insert

    HCoV-229E-Spike (S )
    

    Use

    Gateway-compatible entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pOGG043

    Plasmid

    #133123

    Purpose

    Level 0 PU module synthesised as a fragment (pL0M-PU-PsNifH). Synthetic consensus promoter to drive nodule-specific expression in biovars and strains of R. leguminosarum.

    Depositor

    Philip Poole

    Article

    Mendoza-Suarez et al Proc Natl Acad Sci U S A. 2020 May 5;117

    Insert

    PsNifH Synthetic consensus nifH promoter
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Domesticated for Golden Gate cloning

    Promoter

    Available since

    Dec. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pBABE hygro MEN1 WT

    Plasmid

    #11024

    Depositor

    Matthew Meyerson

    Article

    Hughes et al Mol Cell. 2004 Feb 27. 13(4):587-97.

    Insert

    MEN1 (MEN1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

43. pOPS0381

    Plasmid

    #133231

    Purpose

    Reporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.

    Depositor

    Philip Poole

    Article

    Mendoza-Suarez et al Proc Natl Acad Sci U S A. 2020 May 5;117

    Insert

    constructed with constitutive promoter PsNifH (pOGG043), sfGFP (pOGG037) and T-pharma (pOGG003)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Domesticated for Golden Gate cloning

    Promoter

    Available since

    April 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. TDP-43_CTD_Q331K

    Plasmid

    #98673

    Purpose

    Expresses 6xHis-tagged C-terminal domain of TDP-43 with Q331K

    Depositor

    Nicolas Fawzi

    Article

    Conicella et al Structure. 2016 Sep 6;24(9):1537-49. doi

    Insert

    TDP-43 (TARDBP Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Q331K

    Promoter

    Available since

    July 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pFP5370 ss-FIREmate-HA- KDEL-IVS-IRES- TNF-SBP- mCherry-FIREtag

    Plasmid

    #216696

    Purpose

    Synchronize trafficking of TNFalpha from the ER (RUCH system)

    Depositor

    Franck Perez

    Article

    Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

    Inserts

    FIREmate-KDEL
    Tumor Necrosis Factor alpha (TNFa)
    

    Use

    Tags

    FIREtag, Streptavidin Binding Protein (SBP), and …

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pIDS-RESTwt full-length

    Plasmid

    #109161

    Purpose

    Encodes human full-length REST/NRSF to be expressed in a baculovirus/insect cell expression system

    Depositor

    Monika Golas

    Article

    Inui et al Protein Sci. 2017 May;26(5):997-1011. do

    Insert

    human full-length REST/NRSF (REST Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    p10

    Available since

    Jan. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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47. pAAV-CMV-SauriCas9-KKH-Puro

    Plasmid

    #135966

    Purpose

    Expresses SauriCas9-KKH and puromycin resistance genes, and cloning backbone for sgRNA

    Depositor

    Yongming Wang

    Article

    Hu et al PLoS Biol. 2020 Mar 30;18(3):e3000686. d

    Type

    Empty backbone

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pcDNA3- myc NES-mCE(K294A) NLS

    Plasmid

    #82467

    Purpose

    Expresses cytoplasmic restricted form of myc tagged mRNA capping enzyme

    Depositor

    Daniel Schoenberg

    Article

    Otsuka et al Mol Cell Biol. 2009 Apr;29(8):2155-67. d

    Insert

    NLS deleted from mouse Capping Enzyme (Rngtt Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    deletion of KRKY from 573-657

    Promoter

    CMV

    Available since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pET 2C-T10-dCasX

    Plasmid

    #126418

    Purpose

    E. coli expression vector for inactive casX (triple mutations)--Used for CasX protein purification

    Depositor

    Jennifer Doudna

    Article

    Liu et al Nature. 2019 Feb 4. pii: 10.1038/s41586-

    Insert

    DpbCasX
    

    Use

    CRISPR

    Tags

    His-MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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50. PCMV-intron myc Rab1a S25N

    Plasmid

    #46777

    Purpose

    expression of S25N Rab1a in mammalian cells

    Depositor

    Terry Hébert

    Article

    Dupre et al J Biol Chem. 2006 Nov 10;281(45):34561-7

    Insert

    Rab1a S25N mutant (RAB1A Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    S25N (dominant negative)

    Promoter

    CMV

    Available since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only