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Addgene

We narrowed to 34,448 results for: CaS

Showing: 8601 - 8650 of 34448 results
  1. pTJK635

    Plasmid
    #138009
    Purpose
    SIX1 knockout
    Depositor
    Insert
    SIX1 sgRNA2 (SIX1 Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    None
    Promoter
    U6
    Available since
    May 18, 2020
    Availability
    Academic Institutions and Nonprofits only
  2. pTJK643

    Plasmid
    #138011
    Purpose
    SIX1 knockout
    Depositor
    Insert
    SIX1 sgRNA1 (SIX1 Human)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    None
    Promoter
    U6
    Available since
    May 18, 2020
    Availability
    Academic Institutions and Nonprofits only
  3. pTJK642

    Plasmid
    #138013
    Purpose
    SIX1 CRISPRi
    Depositor
    Insert
    SIX1 sgRNA13 (SIX1 Human)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    None
    Promoter
    U6
    Available since
    May 18, 2020
    Availability
    Academic Institutions and Nonprofits only
  4. pORFE1052

    Plasmid
    #112078
    Purpose
    Overexpression of the AtCAS9 nickase in plant under 2x35S promoter
    Insert
    AtCAS9 D10A
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Plant codon optimized nCas9 D10A S. pyogenes
    Promoter
    2x35S
    Available since
    Aug. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
  5. pORFE0014

    Plasmid
    #112076
    Purpose
    AtCAS9 nickase D10A module CDS1ns
    Insert
    AtCAS9 D10A without stop codon
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Plant codon optimized dCas9 D10A S. pyogenes
    Promoter
    Available since
    Aug. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
  6. pCDF-5xT7-TtCsm

    Plasmid
    #128572
    Purpose
    For expression of T. thermophilus Csm in E. coli (Csm1-5)
    Depositor
    Inserts
    Csm1
    Csm2, Csm3, Csm4, Csm5
    Use
    Tags
    HRV 3C protease cleavage site and 10xHis tag on C…
    Expression
    Bacterial
    Mutation
    Codon optimized for expression in E. coli and Cod…
    Promoter
    T7 promoter, lac operator
    Available since
    Aug. 6, 2019
    Availability
    Academic Institutions and Nonprofits only
  7. plRL19

    Plasmid
    #163634
    Purpose
    L5 Integrase delivery plasmid to allow integration of L5 attP only plasmids (e.g. plRL61, ID #163633).
    Depositor
    Insert
    L5 Integrase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Mycobacterial Optimized Promoter (MOP) and Tet-On
    Available since
    Aug. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
  8. Opto-CRAC version 1

    Plasmid
    #101245
    Purpose
    Genetically-encoded calcium actuator; mCherry-tagged LOV2-STIM1 chimera that enables optical control of calcium signals and calcium dependent signaling
    Depositor
    Insert
    LOV2 and STIM1 cytosolic fragment
    Use
    Tags
    mCherry
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 30, 2018
    Availability
    Academic Institutions and Nonprofits only
  9. BmR1_04g07470-bio-His

    Plasmid
    #108037
    Purpose
    Expresses enzymatically monobiotinylated full-length BmR1_04g07470 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
    Depositor
    Insert
    BmR1_04g07470
    Use
    Tags
    ratCD4d3+4,biotinylation sequence, 6xHis
    Expression
    Mammalian
    Mutation
    all potential N-linked glycosylation sites (N-X-S…
    Promoter
    CMV
    Available since
    Oct. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
  10. PL-SIN-EOS-C(3+)-EiP

    Plasmid
    #21313
    Purpose
    Lentiviral EOS reporter with Oct3/4 enhancer (x3), expresses EGFP and Puro resistance
    Depositor
    Insert
    Enhanced Green Fluorescence Protein, Puromycin resistance gene
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EOS-C(3+) promoter region
    Available since
    June 1, 2009
    Availability
    Academic Institutions and Nonprofits only
  11. 6xHsa.NFKB:d2EGFP

    Plasmid
    #239990
    Purpose
    Destabilized green fluorescent protein (d2EGFP) under transcriptional control of NF-kB activity
    Insert
    Destabilized EGFP
    Use
    Tags
    Expression
    Mutation
    Promoter
    cfos minimal promoter
    Available since
    June 27, 2025
    Availability
    Academic Institutions and Nonprofits only
  12. pHSN6I01

    Plasmid
    #50587
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; expresses dCas9-KRAB, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance
    Depositor
    Inserts
    dCas9-KRAB
    gRNA scaffold
    Use
    CRISPR; Plant expression
    Tags
    3xFLAG, HA, and NLS
    Expression
    Mutation
    dCas9 that is defective in DNA cleavage; the maiz…
    Promoter
    2×35Sp and AtU6-26p
    Available since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
  13. pHSN6A01

    Plasmid
    #50586
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; expresses dCas9-VP64, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance
    Depositor
    Inserts
    dCas9-VP64
    gRNA scaffold
    Use
    CRISPR; Plant expression
    Tags
    3xFLAG, HA, and NLS
    Expression
    Mutation
    dCas9 = defective in DNA cleavage; 4×minimal VP16…
    Promoter
    2×35Sp and AtU6-26p
    Available since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
Showing: 8601 - 8650 of 34448 results