8,883 results

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-3.6kb human IFN-gamma luc

Plasmid

#17599

Depositor

Howard Young

Article

Gonsky et al J Immunol. 2000 Feb 1. 164(3):1399-407.

Insert

IFN gamma promoter (IFNG Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

-3.6 kb to +70 of promoter

Available Since

April 3, 2008

Availability

Academic Institutions and Nonprofits only

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pEYFP-Mitotrap

Plasmid

#46942

Purpose

Expresses Mitotrap in mammalian cells

Depositor

Margaret Robinson

Article

Robinson et al Dev Cell. 2010 Feb 16;18(2):324-31. doi:

Insert

Mitotrap (MTOR Human, Synthetic, Budding Yeast, Aequorea victoria)

Tags

This is a fusion between a yeast mitochondrial ta…

Expression

Mammalian

Available Since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only

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pQUAST

Plasmid

#24349

Depositor

Liqun Luo

Article

Potter et al Cell. 2010 Apr 30. 141(3):536-48.

Insert

5xQUAS-Pmin promoter

Expression

Insect

Available Since

April 12, 2010

Availability

Academic Institutions and Nonprofits only

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pCruzHA SIRT1

Plasmid

#10962

Depositor

Toren Finkel

Article

Nemoto et al J Biol Chem. 2005 Apr 22. 280(16):16456-

Insert

SIRT1 (Sirt1 Mouse)

Tags

HA

Expression

Mammalian

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

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IL-4 promoter luciferase

Plasmid

#12195

Depositor

Anjana Rao

Article

Macian et al EMBO J. 2000 Sep 1. 19(17):4783-95.

Insert

IL-4 promoter (Il4 Mouse)

Use

Luciferase

Tags

luciferase

Expression

Bacterial

Available Since

July 20, 2006

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-hAsCpf1(TYCV) (pY210)

Plasmid

#89351

Purpose

Expresses humanized AsCpf1 variant that recognizes TYCV PAMs

Depositor

Feng Zhang

Article

Gao et al Nat Biotechnol. 2017 Jun 5. doi: 10.1038

Insert

Cpf1, RR variant

Use

CRISPR

Tags

NLS and NLS-3xHA

Expression

Mammalian

Mutation

S542R/K607R

Promoter

CMV

Available Since

June 5, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP PICH (Nigg CB62)

Plasmid

#41163

Depositor

Erich Nigg

Article

Baumann et al Cell. 2007 Jan 12;128(1):101-14.

Insert

PICH (ERCC6L Human)

Tags

eGFP

Expression

Mammalian

Promoter

CMV

Available Since

June 7, 2013

Availability

Academic Institutions and Nonprofits only

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IRES-GFP FoxG1

Plasmid

#14935

Depositor

Joan Massague

Article

Seoane et al Cell. 2004 Apr 16. 117(2):211-23.

Insert

FoxG1 (FOXG1 Human)

Tags

IRES-GFP

Expression

Mammalian

Available Since

May 17, 2007

Availability

Academic Institutions and Nonprofits only

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mRFP-human APPL1

Plasmid

#22202

Depositor

Pietro De Camilli

Article

Erdmann et al Dev Cell. 2007 Sep . 13(3):377-90.

Insert

Adaptor protein containing PH domain, PTB domain and Leucine zipper motif (APPL1 Human)

Tags

mRFP

Expression

Bacterial and Mammalian

Available Since

Oct. 9, 2009

Availability

Academic Institutions and Nonprofits only

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pENTR4-GFP-C1 (w392-1)

Plasmid

#17396

Purpose

Gateway entry vector for N-term eGFP fusion, frame C1

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Insert

GFP

Use

Entry vector

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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human p53-(94-312)

Plasmid

#24866

Depositor

Cheryl Arrowsmith

Article

Ayed et al Nat Struct Biol. 2001 Sep . 8(9):756-60.

Insert

p53 (TP53 Human)

Tags

His

Expression

Bacterial

Mutation

Contains aa 94-312.

Available Since

Aug. 5, 2010

Availability

Academic Institutions and Nonprofits only
tetO-Hand2

Plasmid

#46028

Depositor

John Gearhart

Article

Addis et al J Mol Cell Cardiol. 2013 Apr 13;60C:97-1

Insert

Hand2 (Hand2 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

tetO

Available Since

July 22, 2013

Availability

Academic Institutions and Nonprofits only

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pU6-sgCXCR4-2

Plasmid

#46917

Purpose

Human pSico-based U6 vector containing murine U6 promoter and sgRNA targeting endogenous CXCR4 gene

Depositor

Stanley Qi, Jonathan Weissman

Article

Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

Inserts

sgCXCR4 -2 (CXCR4 Human)
Puromycin resistance and mCherry

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

CMV and U6

Available Since

Oct. 1, 2013

Availability

Academic Institutions and Nonprofits only

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pMBI

Plasmid

#17816

Depositor

Jay Keasling

Article

Martin et al Nat Biotechnol. 2003 Jul . 21(7):796-802

Insert

mevalonate kinase, phosphomevalonate kinase, mevalonate pyrophosphate decarboxylatse, IPP isomerase

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 28, 2008

Availability

Academic Institutions and Nonprofits only
pNCS-CyOFP

Plasmid

#74278

Purpose

Constitutive expression of CyOFP in bacteria

Depositor

Michael Lin

Article

Chu et al Nat Biotechnol. 2016 May 30. doi: 10.103

Insert

CyOFP

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

March 29, 2016

Availability

Academic Institutions and Nonprofits only

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2XMyc-LRRK2-3XKD

Plasmid

#25366

Depositor

Mark Cookson

Article

J Biol Chem. 2008 Jun 13;283(24):16906-1

Insert

LRRK2 (LRRK2 Human)

Tags

2XMyc

Expression

Mammalian

Mutation

"Kinase Dead" Lysine 1906 mutated to A…

Available Since

June 29, 2010

Availability

Academic Institutions and Nonprofits only

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paavCAG-pre-mGRASP-mCerulean-2A-nls-mCherry

Plasmid

#34911

Depositor

Jinhyun Kim

Article

Kim et al Nat Methods. 2011 Dec 4;9(1):96-102. doi

Insert

presynaptic mGRASP

Use

AAV

Promoter

CAG

Available Since

Nov. 15, 2013

Availability

Academic Institutions and Nonprofits only

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CBFRE-EGFP

Plasmid

#17705

Depositor

Nicholas Gaiano

Article

Mizutani et al Nature. 2007 Sep 20. 449(7160):351-5.

Insert

CBFRE

Use

Adenoviral

Tags

EGFP

Expression

Mammalian

Available Since

April 15, 2008

Availability

Academic Institutions and Nonprofits only

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Bmi1-overexpression

Plasmid

#21577

Depositor

Sally Temple

Article

Fasano et al Cell Stem Cell. 2007 Jun 7. 1(1):87-99.

Insert

Bmi-1 (Bmi1 Mouse)

Use

Lentiviral

Tags

ires-eGFP

Expression

Mammalian

Available Since

June 7, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pDisplay-AP-CFP-TM

Plasmid

#20861

Depositor

Alice Ting

Article

Howarth et al Proc Natl Acad Sci U S A. 2005 May 24. 1

Insert

AP-CFP-TM

Tags

CFP, HA, and myc

Expression

Mammalian

Available Since

April 21, 2009

Availability

Academic Institutions and Nonprofits only

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pFLPo

Plasmid

#13792

Purpose

Expression of mouse codon-optimized FLP recombinase

Depositor

Philippe Soriano

Article

Raymond et al PLoS One. 2007 Jan 17;2(1):e162. doi: 10

Insert

FLPo

Use

Cloning vector

Expression

Bacterial

Available Since

Jan. 16, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
Thy1 promoter construct

Plasmid

#20736

Depositor

Joshua Sanes

Article

Feng et al Neuron. 2000 Oct . 28(1):41-51.

Insert

Thy1 promoter (Thy1 Mouse)

Expression

Mammalian

Available Since

April 14, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
M38 TOP-dGFP

Plasmid

#17114

Depositor

Randall Moon

Insert

Tcf/Lef sites (Ctnnb1 )

Tags

GFP

Available Since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pAMPK alpha2 K45R

Plasmid

#15992

Depositor

Morris Birnbaum

Article

Mu et al Mol Cell. 2001 May . 7(5):1085-94.

Insert

AMPK alpha2 (Prkaa2 Rat)

Tags

Myc

Expression

Mammalian

Mutation

K45R

Promoter

CMV

Available Since

Oct. 26, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
MigR1-AE

Plasmid

#12431

Depositor

Dong-Er Zhang

Article

Yan et al Proc Natl Acad Sci U S A. 2004 Dec 7. 10

Insert

AML1-ETO fusion (RUNX1, RUNX1T1 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Available Since

June 2, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
myc mTOR kinase dead

Plasmid

#8482

Depositor

David Sabatini

Insert

mTOR (Mtor Rat)

Tags

myc

Expression

Mammalian

Mutation

D2357E V2364I

Available Since

June 20, 2005

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-C1-PRKAA1

Plasmid

#30305

Depositor

Jay Brenman

Article

Kazgan et al Mol Biol Cell. 2010 Oct 1. 21(19):3433-4

Insert

AMP-activated Protein Kinase α1 (Prkaa1 Rat)

Tags

GFP

Expression

Mammalian

Available Since

July 21, 2011

Availability

Academic Institutions and Nonprofits only

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pSEZ-1

Plasmid

#51868

Purpose

Expression of cpYFP with a mitochondrial-targeting sequence (mt-cpYFP) in C. elegans

Depositor

Meng-Qiu Dong

Article

Shen et al Nature. 2014 Feb 12. doi: 10.1038/nature

Insert

psdhb-1::mtLS-cpYFP

Expression

Worm

Promoter

sdhb-1

Available Since

March 26, 2014

Availability

Academic Institutions and Nonprofits only

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pSKBAD2

Plasmid

#15335

Depositor

Hideo Iwai

Article

Iwai et al FEBS Lett. 2006 Mar 20. 580(7):1853-8.

Insert

DnaE C-intein

Tags

GB1

Expression

Bacterial

Available Since

Dec. 14, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pVenus-VSVG

Plasmid

#11914

Depositor

Jennifer Lippincott-Schwartz

Article

Presley et al Nature. 1997 Sep 4. 389(6646):81-5.

Insert

VSVG

Expression

Mammalian

Mutation

F46L, F64L, M153T, V163A, S175G, T203Y, A206K rel…

Available Since

Sept. 13, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-2TK-14-3-3 eta GST

Plasmid

#13277

Depositor

Michael Yaffe

Article

Yaffe et al Cell. 1997 Dec 26. 91(7):961-71.

Insert

14-3-3 eta (YWHAH Human)

Tags

GST

Expression

Bacterial

Available Since

Oct. 7, 2006

Availability

Academic Institutions and Nonprofits only
Nurr1

Plasmid

#35000

Depositor

Malin Parmar

Article

Pfisterer et al Proc Natl Acad Sci U S A. 2011 Jun 21;10

Insert

Nuclear receptor subfamily 4 group A member 2 (Nr4a2 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

March 6, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMVHA E2F2

Plasmid

#24226

Purpose

Mammalian expression of human E2F2 with HA tag

Depositor

Kristian Helin

Article

Lukas et al Mol Cell Biol. 1996 Mar . 16(3):1047-57.

Insert

E2F2 (E2F2 Human)

Tags

HA

Expression

Mammalian

Available Since

Dec. 15, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223 SARS-CoV-2 NSP5

Plasmid

#141259

Purpose

Gateway-compatible Entry vector, with insert of NSP5 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Has Service

Cloning Grade DNA

Insert

NSP5 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway-compatible entry vector

Mutation

Many synonymous changes due to codon optimization

Available Since

April 1, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pRK5 beta1

Plasmid

#16042

Depositor

Filippo Giancotti

Article

Giancotti et al Cell. 1990 Mar 9. 60(5):849-59.

Insert

beta1 (ITGB1 Human)

Expression

Mammalian

Available Since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA3_UnaG-Flag-Sec61B

Plasmid

#83413

Purpose

Expressed N-term tagged UnaG on Sec61B

Depositor

Hyun-Woo Rhee

Article

Park et al ACS Chem Biol. 2016 May 27.

Insert

UnaG-Sec61B (SEC61B Human, Synthetic)

Expression

Mammalian

Available Since

March 9, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pG14-MS2-GFP

Plasmid

#27117

Depositor

Robert Singer

Article

Bertrand et al Mol Cell. 1998 Oct . 2(4):437-45.

Insert

MS2-binding protein

Tags

GFP, HA, and NLS

Expression

Yeast

Available Since

Dec. 20, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
lentiGuide-Crimson

Plasmid

#70683

Purpose

Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and Crimson reporter from EF-1a promoter. Lentiviral backbone.

Depositor

Daniel Bauer

Article

Canver et al Nature. 2015 Sep 16. doi: 10.1038/nature

Inserts

S. pyogenes sgRNA cassette
Crimson Reporter

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

Ef1-a and hU6

Available Since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
BiP-mCherry

Plasmid

#62233

Purpose

Hamster BiP/GRP78/HSPA5 fused to mCherry. Useful for studying BiP mobility and trafficking in live cells.

Depositor

Erik Snapp

Article

Lai et al Mol Biol Cell. 2010 Jun 15;21(12):1909-2

Insert

hamster BiP-mCherry-KDEL

Tags

mCherry

Expression

Mammalian

Promoter

cmv

Available Since

March 25, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
RRL.sin.cPPT.SFFV/Ace2.IRES-puro.WPRE (MT126)

Plasmid

#145839

Purpose

Lentiviral vector genome to ectopically express human Ace2

Depositor

Caroline Goujon

Article

Rebendenne et al J Virol. 2021 Jan 29. pii: JVI.02415-20.

Insert

human Ace2 (ACE2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

SFFV

Available Since

May 13, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge

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