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169,670 results

Showing: 9551 - 9600 of 169670 results

  1. pCDH-PGK

    Plasmid
    #72268
    Purpose
    Express gene of interest under PGK (phosphoglycerate kinase I) promoter
    Depositor
    Kazuhiro Oka
    Type
    Empty backbone
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    PGK
    Available Since
    Jan. 11, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    72268-plasmid-map-sequence-id-134573

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  2. FUGW-VGLUT1-pHluorin

    Plasmid
    #207470
    Purpose
    Lentiviral expression of VGLUT1-pHluorin
    Depositor
    Robert Edwards
    Insert
    VGLUT1 (Slc17a7 Rat)
    Use
    Lentiviral
    Tags
    pHluorin
    Available Since
    Sept. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    207470-plasmid-map-sequence-id-410343

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  5. BPK764

    Plasmid
    #65767
    Purpose
    Bacterial expression plasmid for SpCas9 & sgRNA (need to clone spacer into BsaI sites): T7-humanSpCas9-NLS-3xFLAG-T7-BsaIcassette-Sp-sgRNA
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9-NLS-3XFlag, and SpCas9 gRNA
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Bacterial
    Promoter
    T7 (x2)
    Available Since
    June 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    65767-plasmid-map-sequence-id-471631

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  7. p663-UBC-V5-3xHA-miniTurbo-3XNLS-STOP_IDG-K

    Plasmid
    #158160
    Purpose
    Gateway destination clone of miniTurbo-3XNLS (as control) tagged with N-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    V5-3xHA-miniTurbo-3XNLS
    Use
    Lentiviral; Gateway destination
    Tags
    V5
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available Since
    Aug. 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    158160-plasmid-map-sequence-id-309971

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  12. MLRV2: NF-kb-SMAD-DBE-P53-AP1

    Plasmid
    #124536
    Purpose
    Multiplex luciferase reporter vector, with luciferase reporters for the pathways NFKB, SMAD, FOXO, P53 and AP1
    Depositor
    Koen Venken
    Article
    Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1
    Inserts
    5 copies of the NF-kb DNA binding motif
    7xSMAD_RE::FLuc
    3xDBE_BE::Renilla (FOXO)
    2xP53_RE::NLuc
    6xAP-1_RE::GrRenilla
    Use
    Luciferase and Synthetic Biology
    Expression
    Mammalian
    Available Since
    June 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    124536-plasmid-map-sequence-id-272178

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  14. PB-NES-ZapCV2

    Plasmid
    #222950
    Purpose
    Genetically-encoded Cytosolic Cyan-Yellow Zinc FRET sensor. Useful for detecting free zinc ion levels in the cytosol (in vitro Kd ~2.3 nM, n = 0.53)
    Depositor
    Amy Palmer
    Article
    Fiedler et al Anal Chem. 2017 Jan 3;89(1):711-719. doi
    Insert
    NES-ZapCV2
    Tags
    ECFP (Enhanced Cyan Fluorescent Protein), Nuclear…
    Expression
    Mammalian
    Mutation
    ECFP gene is truncated 36 bp at 3' end. Venu…
    Available Since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    222950-plasmid-map-sequence-id-450583

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  19. pET-TRSter

    Plasmid
    #78865
    Purpose
    Expresses rat Thioredoxin Reductase using an open reading frame in fusion with a mutated E. coli-type SECIS element
    Depositor
    Elias Arnér
    Article
    Arner et al J Mol Biol. 1999 Oct 8;292(5):1003-16.
    Insert
    Thioredoxin Reductase (Txnrd1 Rat)
    Tags
    Mutated E. coli-type SECIS element (3’ end of ins…
    Expression
    Bacterial
    Promoter
    T7lac
    Available Since
    Sept. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    78865-plasmid-map-sequence-id-147493

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  25. pHIPH4

    Plasmid
    #117685
    Purpose
    Hansenula polymorpha expression plasmid
    Depositor
    Ida van der Klei
    Insert
    Alcohol Oxidase promoter
    Expression
    Yeast
    Promoter
    Alcohol Oxidase
    Available Since
    Jan. 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    117685-plasmid-map-sequence-id-232223

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  40. pGEX-4T-3-GST-Tev-Af1521-myc

    Plasmid
    #196239
    Purpose
    N-terminal GST fusion of Af1521(WT) with a TEV protease site located between the GST tag and Af1521 and a myc tag on the C terminus
    Depositor
    Robert Sobol
    Article
    Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.
    Insert
    N-terminal GST fusion of Af1521(WT) with a TEV protease site between GST and Af1521 encoding a C-terminal myc tag
    Tags
    GST tag and myc tag
    Expression
    Bacterial
    Available Since
    Aug. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    196239-plasmid-map-sequence-id-398149

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  44. GFP-S100A11

    Plasmid
    #107201
    Purpose
    Expresses GFP-human S100A11 fusion protein for live cell imaging
    Depositor
    Volker Gerke, Ursula Rescher
    Insert
    S100A11 (S100A11 Human)
    Tags
    Green Fluorescent Protein
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    107201-plasmid-map-sequence-id-209813

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Showing: 9551 - 9600 of 169670 results