We narrowed to 26,950 results for: gfp gene

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Cin85-3SH3-pCMV6-AN-GFP

Plasmid

#51137

Purpose

Express turbo GFP tagged three SH3 domains of Cin85 (truncation) in mammalian cells

Depositor

Agnieszka Swiatecka-Urban

Insert

Cin85 (SH3KBP1 Human)

Tags

TurboGFP

Expression

Mammalian

Mutation

contains 3 SH3 domains

Promoter

CMV

Available Since

Nov. 21, 2018

Availability

Academic Institutions and Nonprofits only
pSIN ReP5-GFP-GluR1

Plasmid

#24004

Depositor

Robert Malinow

Article

Kessels et al Nat Neurosci. 2009 Jul . 12(7):888-96.

Insert

GluR1 (Gria1 Rat)

Use

Sindbis virus production

Tags

GFP

Available Since

April 5, 2010

Availability

Academic Institutions and Nonprofits only
pscALPS gag-gfp Blasti

Plasmid

#115803

Purpose

Express gag-gfp fusion driven by SFFV promoter

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

gag-gfp

Use

Lentiviral

Promoter

SFFV

Available Since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO GFP DNAJB2a

Plasmid

#19497

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

DNAJB2a

Tags

GFP

Expression

Mammalian

Available Since

Oct. 7, 2008

Availability

Academic Institutions and Nonprofits only

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AB.pCCL.sin.cPPT.U6.miR-92a-Decoy.hPGK.GFP.WPRE

Plasmid

#46638

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-92a Decoy

Use

Lentiviral

Promoter

hU6

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3.1(+) EGFP-Rac1(G12C)

Plasmid

#224279

Purpose

Cell transfection and expression of Rac1 (G12C)

Depositor

Kevan Shokat

Article

Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

Insert

Rac1 (RAC1 Human)

Expression

Mammalian

Mutation

G12C

Available Since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-IRES-hrGFP-mWnt5a

Plasmid

#89772

Purpose

Expresses mouse Wnt5a in mammalian cells

Depositor

Ken-Ichi Takemaru

Article

Rodriguez et al J Neurosci. 2014 Jul 30;34(31):10285-97.

Insert

mouse Wnt5a (Wnt5a Mouse)

Use

AAV

Promoter

CMV

Available Since

March 13, 2019

Availability

Academic Institutions and Nonprofits only

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pAS_4xBstTyrT(CUA)_EF1 sfGFP150TAG

Plasmid

#174891

Purpose

amber suppression reporter sfGFP150TAG expression, with BstTyr(CUA) amber suppressor tRNA cassette

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

Insert

sfGFP

Expression

Mammalian

Mutation

150TAG in sfGFP

Promoter

EF1

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pHAGE-FKBP-GFP-SINTBAD

Plasmid

#216840

Purpose

Stably express FKBP-GFP-tagged SINTBAD for CID induced mitophagy

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv September 25, 202

Insert

SINTBAD (TBKBP1 Human)

Use

Lentiviral

Tags

FKBP-GFP

Expression

Mammalian

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pPRISM-TagRFP-gcry-eGFP

Plasmid

#117767

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

TagRFP

Use

Synthetic Biology

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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pKSR1-CA3-EGFP (C-KSR)

Plasmid

#217753

Purpose

Fluorescent reporter for ceramide (mammalian expression)

Depositor

Paula Nunes-Hasler

Article

Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do

Insert

Kinase suppressor of ras 1 CA3 domain (KSR1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

CA3 domain aa 317-400 plus vector-based linker LE…

Promoter

CMV

Available Since

June 10, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pESC-URA-Rnq1-GFP

Plasmid

#21051

Depositor

Judith Frydman

Article

Kaganovich et al Nature. 2008 Aug 28. 454(7208):1088-95.

Insert

Rnq1 (RNQ1 Budding Yeast)

Use

Yeast/bacteria shuttle

Tags

GFP

Expression

Yeast

Available Since

May 28, 2009

Availability

Academic Institutions and Nonprofits only
pTYF-mCMV/GfaABC1D-EGFP

Plasmid

#19976

Depositor

Sergey Kasparov

Article

Liu et al BMC Biotechnol. 2008 May 16;8:49. doi: 1

Insert

mCMVGfaABC1D-EGFP

Expression

Mammalian

Available Since

Aug. 5, 2009

Availability

Academic Institutions and Nonprofits only
pQCXIP-NLS-Vx3-mEGFP

Plasmid

#35529

Depositor

Robert Cohen

Article

Sims et al Nat Methods. 2012 Feb 5. doi: 10.1038/nm

Insert

NLS-Vx3

Use

Retroviral

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

April 6, 2012

Availability

Academic Institutions and Nonprofits only

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11K-cyto-->R GLUT1-GFP

Plasmid

#200096

Purpose

human GLUT1 with K-->R mutations for all 11 cytosolic lysines and a C-terminal GFP tag in pQCXIP vector

Depositor

Jason MacGurn

Article

Qualls-Histed et al iScience. 2023 Feb 6;26(3):106150. doi:

Insert

GLUT1 (SLC2A1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

K-->R mutations of amino acids 6, 7, 225, 229,…

Promoter

CMV

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pAW-CD8-NbVHH05-GFP

Plasmid

#171576

Purpose

Expresses GFP-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-GFP) in fly cell cytomembrane

Depositor

Norbert Perrimon, Hidde Ploegh

Article

Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

Insert

CD8-NbVHH05-GFP (UBE2J1 Human)

Tags

CD8 and GFP

Expression

Insect

Promoter

fly actin5C promoter

Available Since

June 30, 2021

Availability

Academic Institutions and Nonprofits only

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pBMN mEGFP-OPTN(F178A)

Plasmid

#119681

Purpose

For mammalian expression of LIR mutant OPTN

Depositor

Michael Lazarou

Article

Padman et al Nat Commun. 2019 Jan 24;10(1):408. doi:

Insert

OPTN (OPTN Human)

Use

Retroviral

Tags

mEGFP

Expression

Mammalian

Mutation

F178A

Available Since

Feb. 19, 2019

Availability

Academic Institutions and Nonprofits only

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KIF5C(1-560)-2xmCitrine-GFP11

Plasmid

#61667

Purpose

Tandem mCitrine-labeled, truncated kinesin-1 fused to split GFP linker

Depositor

Kristen Verhey

Article

Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

Insert

KIF5C(1-560)-2xmCitrine-GFP11 (Kif5c Rat)

Expression

Mammalian

Available Since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

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MSCV-Axin-IRES-GFP

Plasmid

#14716

Depositor

Tannishtha Reya

Article

Reya et al Nature. 2003 May 22. 423(6938):409-14.

Insert

Axin (Axin1 Mouse)

Use

Retroviral

Tags

IRES-GFP

Expression

Mammalian

Available Since

Aug. 28, 2009

Availability

Academic Institutions and Nonprofits only
pCAG-EGFP/RFP-int

Plasmid

#19818

Depositor

Zuoshang Xu

Article

Qiu et al BMC Biotechnol. 2008 Oct 7;8:77. doi: 10

Type

Empty backbone

Use

Cre/Lox and RNAi

Expression

Mammalian

Available Since

Nov. 21, 2008

Availability

Academic Institutions and Nonprofits only
pAAV-GFAP-eGFP-shAqp4-4

Plasmid

#223225

Purpose

mir30 based shRNA strategy, shRNA targeting mouse Aqp4

Depositor

Li-Huei Tsai

Article

Murdock et al Nature. 2024 Mar;627(8002):149-156. doi:

Insert

shAqp4 (Aqp4 Mouse)

Use

AAV

Available Since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-GFAP-eGFP-shAqp4-3

Plasmid

#223224

Purpose

mir30 based shRNA strategy, shRNA targeting mouse Aqp4

Depositor

Li-Huei Tsai

Article

Murdock et al Nature. 2024 Mar;627(8002):149-156. doi:

Insert

shAqp4 (Aqp4 Mouse)

Use

AAV

Available Since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-GFAP-eGFP-shAqp4-1

Plasmid

#223223

Purpose

mir30 based shRNA strategy, shRNA targeting mouse Aqp4

Depositor

Li-Huei Tsai

Article

Murdock et al Nature. 2024 Mar;627(8002):149-156. doi:

Insert

shAqp4 (Aqp4 Mouse)

Use

AAV

Available Since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-GFAP-eGFP-shLacZ

Plasmid

#223222

Purpose

mir30 based shRNA strategy, control shRNA

Depositor

Li-Huei Tsai

Article

Murdock et al Nature. 2024 Mar;627(8002):149-156. doi:

Insert

shLacZ

Use

AAV

Available Since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW yosfGFP-T2A-mCherry

Plasmid

#118448

Purpose

Produces equimolar expression of yeast codon-optimized sfGFP and mCherry

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

yosfGFP-T2A-mCherry

Expression

Yeast

Available Since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/TO-N-mEGFP-PARP6

Plasmid

#178005

Purpose

Expresses N-terminally mEGFP-tagged PARP6

Depositor

Karla Feijs

Article

Weixler et al Life Sci Alliance. 2022 Nov 11;6(1):e202

Insert

PARP6 (PARP6 Human)

Expression

Mammalian

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/TO-N-mEGFP-PARP11

Plasmid

#177998

Purpose

Expresses N-terminally mEGFP-tagged PARP11

Depositor

Karla Feijs

Article

Weixler et al Life Sci Alliance. 2022 Nov 11;6(1):e202

Insert

PARP11 (PARP11 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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MSCV-Neo-GFP/Foxa1

Plasmid

#105506

Purpose

MSCV-driven retroviral Foxa1 expression

Depositor

Christopher Vakoc

Article

Roe et al Cell. 2017 Aug 24;170(5):875-888.e20. do

Insert

Foxa1 (Foxa1 Mouse)

Use

Retroviral

Available Since

Feb. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pET22b-mEB2-eGFP-6xHis

Plasmid

#130982

Purpose

Bacterial expression of mouse EB2-GFP-6xHis for protein purification.

Depositor

Anne Straube

Article

Roth et al J Cell Sci. 2018 Oct 31;132(4). pii: jcs

Insert

EB2-GFP-6xHis (Mapre2 Mouse)

Tags

eGFP-6xHis

Expression

Bacterial

Promoter

T7

Available Since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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PITX3-2A-eGFP-PGK-Puro

Plasmid

#31943

Depositor

Rudolf Jaenisch

Article

Hockemeyer et al Nat Biotechnol. 2011 Jul 7. doi: 10.1038

Insert

PITX3 (PITX3 Human)

Use

Targeting donor

Tags

eGFP

Promoter

-

Available Since

Sept. 13, 2011

Availability

Academic Institutions and Nonprofits only

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pME-EGFP-P2A-APEX2-NS

Plasmid

#108895

Purpose

Multisite gateway vector for 5' tagging with APEX2 (includes cytoplasmic GFP reporter)

Depositor

Rob Parton

Article

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

EGFP-P2A-APEX2

Use

Multisite gateway

Available Since

May 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
PB_EF1a-CasRx-msfGFP-2A-Blast

Plasmid

#226010

Purpose

CasRx protein for RNA targeting in piggyBac backbone

Depositor

Silvana Konermann

Article

Wei et al Cell Syst. 2023 Dec 20;14(12):1087-1102.

Insert

CasRx RNA-targeting nuclease

Use

CRISPR

Tags

msfGFP

Expression

Mammalian

Available Since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pLX307 P2A GFP PRDM14

Plasmid

#193676

Purpose

Constitutive lentiviral expression of PRDM14

Depositor

William Hahn

Article

Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

Insert

PRDM14 (PRDM14 Human)

Use

Lentiviral

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
CMV:eGFP-p2A-HA-GPR151(p.Arg95Ter)

Plasmid

#190134

Purpose

expresses eGFP-p2A-HA-GPR151 (pArg95Ter variant) in mammalian cells

Depositor

Joshua Knowles

Article

Bielczyk-Maczynska et al Nat Commun. 2022 Dec 1;13(1):7408. doi:

Insert

eGFP-p2A-HA-GPR151(p.Arg95Ter)

Use

Lentiviral

Tags

HA, eGFP, and p2A

Expression

Mammalian

Mutation

p.Arg95Ter mutation

Promoter

CMV

Available Since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
CB6-GFP-TAOK2 (K57A)

Plasmid

#197114

Purpose

Expression of kinase-deficient GFP-tagged TAOK2 (K57A) / PSK1 (K57A) in mammalian cells

Depositor

Jonathan Morris

Article

Garg et al J Cell Sci. 2020 Mar 16;133(6):jcs235895

Insert

TAOK2 (K57A) (TAOK2 Human)

Tags

GFP

Expression

Mammalian

Mutation

Changed K 57 to A

Promoter

CMV

Available Since

March 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLPCX roGFP2-Prx2(C172A)

Plasmid

#158228

Purpose

Genetically encoded fluorescent H2O2 sensor roGFP2-Prx2(C172A) for cytosolic expression in mammalian cells (for stable cell line generation)

Depositor

Tobias Dick

Article

Pastor-Flores et al Proc Natl Acad Sci U S A. 2020 Jun 29. p

Insert

roGFP2-Prx2(C172A)

Use

Retroviral

Expression

Mammalian

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA5 FRT TO GFP hBub1 WT

Plasmid

#59812

Purpose

Allows the integration of GFP hBub1 in the genome and Tet-inducible expression.

Depositor

Stephen Taylor

Article

Perera et al J Cell Sci. 2010 Mar 1;123(Pt 5):653-9.

Insert

Bub1 (BUB1 Human)

Tags

EmGFP

Expression

Mammalian

Promoter

hybrid human cytomegalovirus (CMV)/TetO2 promoter

Available Since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pHR_TRE3GS-->GssGFP-P2A-NLSIFP

Plasmid

#162235

Purpose

Lentiviral vector for the expression of TRE3GS-->GssGFP-P2A-NLSIFP in mammalian cells

Depositor

Wendell Lim

Article

Toda et al Science. 2020 Oct 16;370(6514):327-331.

Insert

GFP - IFP

Use

Lentiviral

Expression

Mammalian

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-NPM NESM-NLS1D

Plasmid

#13288

Depositor

Xin Wang

Article

Wang et al Nat Cell Biol. 2005 Aug . 7(8):823-30.

Insert

NPM (NPM1 Human)

Tags

Flag

Expression

Mammalian

Mutation

L100A, L102A, deletion of aa134-aa142

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only
pSICO-CPSF6-358-eGFP

Plasmid

#110693

Purpose

Expresses a truncated form of CPSF6 (CPSF6-358 originally described in Lee et al. Cell Host Microbe 2010) fused with eGFP

Depositor

Zandrea Ambrose

Article

Ning et al J Virol. 2018 Apr 11. pii: JVI.00368-18.

Insert

CPSF6-eGFP (CPSF6 )

Use

Lentiviral

Tags

EGFP

Mutation

Truncation of CPSF6 isoform 2 at amino acid 358

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pSMVP-Cas9C-P2A-turboGFP

Plasmid

#80935

Purpose

Plasmid that expresses Cas9C in mammalian cells; co-expresses turboGFP.

Depositor

George Church

Article

Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

Insert

Cas9C

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Available Since

Oct. 3, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pKLV2-U6gRNA5(Empty)-PGKGFP2ABFP-W

Plasmid

#67983

Purpose

Cas9 activity reporter (control) with GFP and BFP

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

U6gRNA cassette, PGKGFP2ABFP cassette, WPRE

Use

CRISPR and Lentiviral

Mutation

Deleted BbsI site within WPRE, modified BFP

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR-SIN-GFP-PTEN-WT

Plasmid

#30391

Depositor

Todd Waldman

Article

Kim et al Mol Cell Biol. 2011 Jul . 31(13):2756-71

Insert

GFP-PTEN-WT (PTEN Human)

Expression

Mammalian

Mutation

GFP-PTEN-WT

Available Since

Sept. 1, 2011

Availability

Academic Institutions and Nonprofits only
pDEST-FRT-TO-GFP-PALB2 KR

Plasmid

#71107

Purpose

mammalian expression of GFP-PALB2 mutant. (first 8 Lysine residues mutated to Arginine)

Depositor

Daniel Durocher

Article

Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

Insert

PALB2 (PALB2 Human)

Tags

GFP

Expression

Mammalian

Mutation

first 8 Lysine residues mutated to Arginine (K14-…

Promoter

CMV/TO

Available Since

Dec. 2, 2015

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
GFP-Tollip M240A,F241A

Plasmid

#178051

Purpose

GFP fused to human Tollip cDNA carrying mutations in the CUE domain. Localisation and expression in mammalian cells.

Depositor

David Tumbarello

Article

Ryan et al EMBO J. 2020 Jun 2;39(11):e102539. doi:

Insert

Toll-Interacting Protein (TOLLIP Human)

Tags

GFP

Expression

Mammalian

Mutation

Changed Methionine 240 to Alanine and changed Phe…

Promoter

CMV

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3 Connexin43-GFP-APEX

Plasmid

#44440

Depositor

Alice Ting

Article

Martell et al Nat Biotechnol. 2012 Oct 21. doi: 10.103

Insert

Connexin43 -EGFP - pea APEX

Expression

Mammalian

Mutation

K14D, W41F, E112K mutations on APEX

Promoter

CMV

Available Since

May 14, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC19-URA3-3'SEC13-GFP

Plasmid

#25445

Depositor

Benjamin Glick

Article

Rossanese et al J Cell Biol. 1999 Apr 5. 145(1):69-81.

Insert

Sec13-Green Fluorescent Protein

Expression

Yeast

Available Since

June 24, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-hSyn-eGFP-SynaptoZip

Plasmid

#122525

Purpose

AAV expression of the synaptic activity-marker SynaptoZip fused to eGFP driven by human Synapsin promoter

Depositor

Antonio Malgaroli

Article

Ferro et al Nat Commun. 2017 Oct 31;8(1):1229. doi:

Insert

eGFP-SynaptoZip (Vamp2 Rat)

Use

AAV

Tags

Myc and eGFP

Expression

Mammalian

Promoter

hSyn

Available Since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pSC101-GFPmut2-mScarlet-I TAG

Plasmid

#208188

Purpose

nonsense mistranslation, TAG stop

Depositor

Tanel Tenson

Article

Hinnu et al Sci Rep. 2024 Mar 22;14(1):6883. doi: 10

Inserts

GFPmut2
mScarlet-I

Use

Reporter

Expression

Bacterial

Mutation

Insertion 6TAG*(stop) 7AGT(Ser)

Promoter

Ptet+dnaK P1

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pSC101-GFPmut2-mScarlet-I TGA

Plasmid

#208189

Purpose

nonsense mistranslation, TGA stop

Depositor

Tanel Tenson

Article

Hinnu et al Sci Rep. 2024 Mar 22;14(1):6883. doi: 10

Inserts

GFPmut2
mScarlet-I

Use

Reporter

Expression

Bacterial

Mutation

Insertion 6TGA*(stop) 7AGT(Ser)

Promoter

Ptet+dnaK P1

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge

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We narrowed to 26,950 results for: gfp gene