19,335 results

1. pSAM_Cam

   Plasmid

   #91570

   Purpose

   Mariner transposon mutagenesis & sequencing vector - Transposon contains KanR and Illumina sequencing adapters. Lac promoter expresses transposase. Chloramphenicol resistance on backbone.

   Depositor

   Harry Mobley

   Article

   Bachman et al MBio. 2015 Jun 9;6(3):e00775. doi: 10.11

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

2. SBE4-Luc

   Plasmid

   #16495

   Purpose

   Luciferase reporter containing four copies of the Smad binding site

   Depositor

   Bert Vogelstein

   Article

   Zawel et al Mol Cell. 1998 Mar . 1(4):611-7.

   Insert

   Smad binding element
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 7, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEZYflag

   Plasmid

   #18700

   Purpose

   pFlag-CMV-2 converted Gateway Destination vector for expressing Flag tagged fusion proteins in mammalian cells

   Depositor

   Yu-Zhu Zhang

   Article

   Guo et al Biotechnol J. 2008 Mar . 3(3):370-7.

   Type

   Empty backbone

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 26, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLVX-XBP1 mNeonGreen NLS

   Plasmid

   #115968

   Purpose

   ER-stress sensor - XBP1 splicing fluorescent reporter with mNeonGreen

   Depositor

   David Andrews

   Article

   Nougarede et al Assay Drug Dev Technol. 2018 Aug 8. doi:

   Insert

   X-box binding protein 1 (XBP1 Synthetic, Human)
   

   Use

   Lentiviral

   Tags

   HA tag, c-myc NLS, and mNeonGreen

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-SMVP-Cas9N

   Plasmid

   #80930

   Purpose

   Expresses Cas9N in mammalian cells.

   Depositor

   George Church

   Article

   Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

   Insert

   Cas9N
   

   Use

   AAV, CRISPR, and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. Lenti-miniTurbo-NLS-Blast

   Plasmid

   #232347

   Purpose

   Lentiviral control vector expressing miniTurboNLS without dCas9

   Depositor

   Ali Shilatifard

   Article

   Cenik et al Mol Cell. 2024 Dec 19;84(24):4929-4944.e

   Insert

   miniTurbo
   

   Use

   Lentiviral

   Tags

   3xHA and 3xSV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRC-CMV-Rev1b

   Plasmid

   #164443

   Purpose

   Encodes HIV-1 Rev accessory protein for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Garcia-Beltran et al Cell. 2021 Jan 21;184(2):476-488.e11. do

   Insert

   HIV-1 Rev
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDONR223_KRAS_p.G12V

   Plasmid

   #81665

   Purpose

   Gateway Donor vector containing KRAS,part of the Target Accelerator Plasmid Collection.

   Depositor

   William Hahn, Jesse Boehm, David Root

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   KRAS (KRAS Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   G12V

   Promoter

   None

   Available since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. VinculinTS

   Plasmid

   #26019

   Depositor

   Martin Schwartz

   Article

   Grashoff et al Nature. 2010 Jul 8. 466(7303):263-6.

   Insert

   Vinculin tension sensor
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 17, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET-Bxb1-His

    Plasmid

    #123132

    Purpose

    Bacterial expression plasmid for wild-type Bxb1 with a C-terminal His-tag.

    Depositor

    David Liu

    Article

    Bessen et al Nat Commun. 2019 Apr 26;10(1):1937. doi:

    Insert

    Bxb1 integrase
    

    Use

    Tags

    6x His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. PB-TO-hNGN2

    Plasmid

    #172115

    Purpose

    Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression

    Depositor

    Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

    Article

    Pantazis et al Cell Stem Cell. 2022 Dec 1;29(12):1685-1

    Inserts

    hNGN2 (NEUROG2 Human)
    puroR-T2A-mycNLS-mTagBFP2
    

    Use

    Tags

    T2A-mycNLS-mTagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a and TRE3G

    Available since

    April 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMSCV-IRES-CFP II (pMIC II)

    Plasmid

    #52109

    Purpose

    a bicistronic IRES-CFP containing retroviral vector, MCS is expanded from pMSCV-IRES-GFP

    Depositor

    Dario Vignali

    Article

    Holst et al Nat Protoc. 2006;1(1):406-17.

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    IRES-ECFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pEGFP-Akt1(WT)

    Plasmid

    #86637

    Purpose

    Expresses eGFP-tagged human Akt1(WT)

    Depositor

    Ivan Yudushkin, Thomas Leonard

    Article

    Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

    Insert

    Akt1 (AKT1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. EBFP-C1

    Plasmid

    #54738

    Purpose

    Localization: C1 Cloning Vector, Excitation: 387, Emission: 450

    Depositor

    Michael Davidson

    Type

    Empty backbone

    Use

    Tags

    EBFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 25, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pAAV-SMVP-Cas9C

    Plasmid

    #80931

    Purpose

    Expresses Cas9C in mammalian cells.

    Depositor

    George Church

    Article

    Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

    Insert

    Cas9C
    

    Use

    AAV, CRISPR, and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pYSEAP

    Plasmid

    #37326

    Depositor

    John Schiller

    Article

    Pastrana et al Virology. 2004 Apr 10;321(2):205-16.

    Insert

    SEAP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    July 23, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCMV3flag8HOIL-1L

    Plasmid

    #50016

    Purpose

    Mammalian expression of human HOIL-1L (RBCK1) with a c-terminal 3xFLAG tag, and hygromycin selection.

    Depositor

    Martin Dorf

    Article

    Fu et al Cell Res. 2013 Sep 24. doi: 10.1038/cr.2

    Insert

    RanBP-type and C3HC4-type zinc finger containing 1 HOIL-1L (RBCK1 Human)
    

    Use

    Tags

    3 x FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 31, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHAGE-DDX3X

    Plasmid

    #116730

    Purpose

    Lentiviral expression of DDX3X

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    DDX3X (DDX3X Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Pcr4-12xMBS-PBS

    Plasmid

    #52984

    Purpose

    provide MBS-PBS alternating sequence

    Depositor

    Robert Singer

    Article

    Wu et al Sci Rep. 2014 Jan 9;4:3615. doi: 10.1038

    Insert

    12xMBS-PBS
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    none

    Available since

    June 23, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAC1802_pmax-RBFOX1N-dCasRx-C

    Plasmid

    #118635

    Purpose

    Transient transfection; Expresses RBFOX1N-dCasRx-C; CAGGS promoter

    Depositor

    Albert Cheng

    Article

    Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

    Insert

    RBFOX1N-dCasRx-C
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAGGS

    Available since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. GST-TEV-FUS

    Plasmid

    #29629

    Depositor

    Aaron Gitler

    Article

    Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

    Insert

    FUS (FUS Human)
    

    Use

    Tags

    GST and TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pFCcGi

    Plasmid

    #59324

    Purpose

    Inducible expression of GFP and constitutive expression of mCherry

    Depositor

    David Holden, Sophie Helaine

    Article

    Figueira et al MBio. 2013 Apr 16;4(2):e00065. doi: 10.1

    Inserts

    mCherry
    EGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Pbad and rpsM

    Available since

    Oct. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAAV_hSyn1_nLightG

    Plasmid

    #187179

    Purpose

    Expresses green norepinephrine indicator nLightG in neuronal cells

    Depositor

    Tommaso Patriarchi

    Article

    Kagiampaki et al Nat Methods. 2023 Sep;20(9):1426-1436. d

    Has Service

    AAV9

    Insert

    nLightG
    

    Use

    AAV

    Tags

    Flag tag

    Expression

    Mutation

    Promoter

    human Synapsin-1

    Available since

    July 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Cre Stoplight 2.4

    Plasmid

    #37402

    Purpose

    Cre activity reporter plasmid; Zsgreen is expressed in cells without Cre, mCherry is expressed with Cre

    Depositor

    Thomas Hughes

    Article

    Yang et al Biotechniques. 2001 Nov;31(5):1036, 1038

    Insert

    Cre Stoplight 2.4
    

    Use

    Tags

    ZsGreen1 and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 1, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pEGFP-N1-MITF-D

    Plasmid

    #38133

    Depositor

    Shawn Ferguson

    Article

    Roczniak-Ferguson et al Sci Signal. 2012 Jun 12;5(228):ra42.

    Insert

    MITF-D (MITF Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    deleted stop codon

    Promoter

    CMV

    Available since

    Aug. 1, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLminP_Luc2P_RE55

    Plasmid

    #90400

    Purpose

    C-REL - NFkB gene reporter

    Depositor

    Ramnik Xavier

    Article

    O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

    Insert

    Firefly luciferase
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CEBPa

    Available since

    Nov. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pMRX-INU-FLAG-FAM134B

    Plasmid

    #128260

    Purpose

    Expresses FAM134B in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Chino et al Mol Cell. 2019 Apr 11. pii: S1097-2765(1

    Insert

    FAM134B (RETREG1 Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA3.1-IDH2-WT

    Plasmid

    #87926

    Purpose

    Expresses IDH2 (wild-type) in mammalian cells

    Depositor

    Reproducibility Project Cancer Biology

    Article

    Showalter et al Elife. 2017 Jun 27;6. pii: e26030. doi:

    Insert

    isocitrate dehydrogenase (NADP(+)) 2, mitochondrial (IDH2 Human)
    

    Use

    Tags

    none

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pFLAG-HHARI

    Plasmid

    #17450

    Depositor

    Dong-Er Zhang

    Article

    Okumura et al Genes Dev. 2007 Feb 1. 21(3):255-60.

    Insert

    HHARI (ARIH1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 5, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLVX-EF1alpha-SARS-CoV-2-nsp6-2xStrep-IRES-Puro

    Plasmid

    #141372

    Purpose

    Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

    Depositor

    Nevan Krogan

    Article

    Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

    Insert

    SARS-CoV-2-nsp6 (ORF1ab SARS-CoV-2)
    

    Use

    Lentiviral

    Tags

    2xStrep

    Expression

    Mammalian

    Mutation

    human codon optimized

    Promoter

    EF1alpha

    Available since

    Sept. 17, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

31. pcDNA3.1-HA-mUbp43

    Plasmid

    #12454

    Depositor

    Dong-Er Zhang

    Article

    Malakhova et al EMBO J. 2006 Jun 7. 25(11):2358-67.

    Insert

    UBP43 (Usp18 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 4, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pMRX-INU-FLAG-CCPG1

    Plasmid

    #128262

    Purpose

    Expresses CCPG1 in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Chino et al Mol Cell. 2019 Apr 11. pii: S1097-2765(1

    Insert

    CCPG1 (CCPG1 Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pEGFP-SF2

    Plasmid

    #17990

    Depositor

    Tom Misteli

    Article

    Phair et al Nature. 2000 Apr 6. 404(6778):604-9.

    Insert

    SF2 (SRSF1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    contains SF2 (aa1-210)

    Promoter

    Available since

    May 13, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. p2T-CMV-ABEmax-BlastR

    Plasmid

    #152989

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

    Insert

    ABE7.10-nSpCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pLEX_305-N-dTAG

    Plasmid

    #91797

    Purpose

    Lentiviral/gateway cloning vector for N-terminally tagging proteins of interest with the dTAG system

    Depositor

    James Bradner, Behnam Nabet

    Article

    Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    FKBP F36V

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK

    Available since

    March 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. AAVS1-Tet-OsTIR1(F74G)-V5

    Plasmid

    #158664

    Purpose

    Tet-OsTIR1(F74G)-V5 donor for integration at the AAVS1 locus

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    Tet-OsTIR1(F74G) AAVS1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    F74G

    Promoter

    Available since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pST_116_LVL2 cam

    Plasmid

    #179332

    Purpose

    NT-CRISPR plasmid for a single gRNA.

    Depositor

    Anke Becker

    Article

    Stukenberg et al Commun Biol. 2022 Mar 25;5(1):265. doi:

    Insert

    Ptac tfoX, Ptet cas9, PJ23106 acrIIA4, Ptet gRNA with sfGFP dropout
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pEGFPC1-human APPL1

    Plasmid

    #22198

    Depositor

    Pietro De Camilli

    Article

    Erdmann et al Dev Cell. 2007 Sep . 13(3):377-90.

    Insert

    Adaptor protein containing PH domain, PTB domain and Leucine zipper motif (APPL1 Human)
    

    Use

    Tags

    GFP

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Oct. 21, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pFA6a-6xGLY-3xFLAG-kanMX6

    Plasmid

    #20754

    Depositor

    Mark Hochstrasser

    Article

    Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

    Type

    Empty backbone

    Use

    Pcr-based c-terminal tagging

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pC0049-EF1a-dPSPCas13b-NES-HIV, H133A/H1058A

    Plasmid

    #103865

    Purpose

    Catalytically inactive PSPCas13b. RNase activity is inactivated by mutation of catalytic histidine residues in the HEPN domains (H133A/H1058A).

    Depositor

    Feng Zhang

    Article

    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

    Insert

    PspCas13b
    

    Use

    CRISPR and Lentiviral

    Tags

    3xHA and HIV NES

    Expression

    Mammalian

    Mutation

    Contains the H133A and H1058A mutations which ina…

    Promoter

    EF1a

    Available since

    Jan. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge