Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

179,521 results

Showing: 8281 - 8320 of 179521 results

  2. pJYS1Peftu

    Plasmid
    #85546
    Purpose
    Constitutive expression of FnCpf1 and recT in C.glutamitum, etfu promoter
    Depositor
    Sheng Yang
    Publication
    Jiang et al Nat Commun. 2017 May 4;8:15179. doi: 10.
    Inserts
    Cpf1
    recT
    Use
    CRISPR; Shuttle vector corynebacterium glutamicum…
    Expression
    Bacterial
    Promoter
    etfu and unknown
    Available since
    May 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    85546-plasmid-map-sequence-id-176968

    Enlarge

  3. eSpCas9(1.1)_No_FLAG_ATP1A1_G2_Dual_sgRNA

    Plasmid
    #86612
    Purpose
    Vector for tandem expression of ATP1A1 G2 sgRNA in combination with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos for second sgRNA using BbsI sites. Px333-like plasmid
    Depositor
    Yannick Doyon
    Publication
    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n
    Insert
    ATP1A1 G2 sgRNA + user-specified sgRNA + FLAGless enhanced specificity Cas9 (1.1)
    Use
    CRISPR; Co-selection via nhej using ouabain
    Expression
    Mammalian
    Mutation
    K848A, K1003A, & R1060A
    Promoter
    CBh
    Available since
    March 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    86612-plasmid-map-sequence-id-168849

    Enlarge

  6. mCyRFP1-C1

    Plasmid
    #84355
    Purpose
    Expresses mCyRFP1 fluorescent protein in an empty backbone
    Depositor
    Ryohei Yasuda
    Publication
    Laviv et al Nat Methods. 2016 Oct 31. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mCyRFP1
    Expression
    Mammalian
    Mutation
    A161K and N41A, reletive to CyRFP1
    Promoter
    CMV
    Available since
    Nov. 22, 2016
    Availability
    Academic Institutions and Nonprofits only
    84355-plasmid-map-sequence-id-160941

    Enlarge

  10. c3GIC9

    Plasmid
    #62191
    Purpose
    col1a1 targeting vector for inducible [TRE3G]-GFP-IRES-Cas9 expression. Contains NsiI cloning site for U6-sgRNA cassettes
    Depositor
    Lukas Dow
    Publication
    Dow et al Nat Biotechnol. 2015 Apr;33(4):390-4. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    humanized S. Pyogenes Cas9
    Use
    CRISPR
    Tags
    3x FLAG
    Promoter
    TRE3G
    Available since
    Aug. 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    62191-plasmid-map-sequence-id-156341

    Enlarge

  11. pcDNA3.0-TRPV4-p2A-ferritin-p2A-mCherry

    Plasmid
    #74309
    Purpose
    Tricistronic expression of TRPV4, ferritin, and mCherry in mammalian cells
    Depositor
    Ali Guler
    Publication
    Wheeler et al Nat Neurosci. 2016 Mar 7. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TRPV4-p2A-ferritin-p2A-mCherry (Trpv4 Human, Rat)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 18, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    74309-plasmid-map-sequence-id-153122

    Enlarge

  16. pEGFP-C3-PLK4-3xFLAG

    Plasmid
    #69837
    Purpose
    This plasmid encodes PLK4 isoform 1 with an N-terminal EGFP tag and C-terminal 3xFLAG tag
    Depositor
    Michel Bornens
    Publication
    Sillibourne et al Mol Biol Cell. 2010 Feb 15;21(4):547-61.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PLK4 (PLK4 Human)
    Tags
    3xFLAG tag and EGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Oct. 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    69837-plasmid-map-sequence-id-123876

    Enlarge

  17. pCITF-KCC2

    Plasmid
    #61404
    Purpose
    Mammalian expression vector for human KCC2 under CAG promoter. Coexpression of TdTomato with IRES sequence
    Depositor
    Franck Polleux
    Publication
    Bortone et al Neuron. 2009 Apr 16;62(1):53-71. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    KCC2 (SLC12A5 Human)
    Tags
    IRES-tdTomato-Flag
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Aug. 26, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    61404-plasmid-map-sequence-id-122754

    Enlarge

  20. GFP-BRDT

    Plasmid
    #65381
    Purpose
    GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.
    Depositor
    Kyle Miller
    Publication
    Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    BRDT (BRDT Human)
    Tags
    EmGFP and V5
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available since
    May 14, 2015
    Availability
    Academic Institutions and Nonprofits only
    65381-plasmid-map-sequence-id-245402

    Enlarge

  21. BiP-mCherry

    Plasmid
    #62233
    Purpose
    Hamster BiP/GRP78/HSPA5 fused to mCherry. Useful for studying BiP mobility and trafficking in live cells.
    Depositor
    Erik Snapp
    Publication
    Lai et al Mol Biol Cell. 2010 Jun 15;21(12):1909-2
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    hamster BiP-mCherry-KDEL
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    cmv
    Available since
    March 25, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    62233-plasmid-map-sequence-id-314153

    Enlarge

  25. mCherry-MAPTau-C-10

    Plasmid
    #55077
    Purpose
    Localization: Microtubules, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    MAPTau (MAPT Human)
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 29, 2014
    Availability
    Academic Institutions and Nonprofits only
    55077-plasmid-map-sequence-id-353040

    Enlarge

  28. mApple-Alpha-V-Integrin-25

    Plasmid
    #54866
    Purpose
    Localization: Focal Adhesions, Excitation: 568, Emission: 592
    Depositor
    Michael Davidson
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Alpha-V Integrin (ITGAV Human)
    Tags
    mApple
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    54866-plasmid-map-sequence-id-514621

    Enlarge

  33. pRK GFP Paxillin

    Plasmid
    #50529
    Purpose
    This plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.
    Depositor
    Kenneth Yamada
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Paxillin (PXN Human)
    Tags
    GFP21
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Jan. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    50529-plasmid-map-sequence-id-203086

    Enlarge

  38. BRD4

    Plasmid
    #38942
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    BRD4 (BRD4 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    38942-plasmid-map-sequence-id-348518

    Enlarge

Showing: 8281 - 8320 of 179521 results