162,316 results

1. Px461-Cas9n-Trp53-sgRNA-beta

   Plasmid

   #88847

   Purpose

   CRISPR KO of Trp53

   Depositor

   Joan Massague

   Article

   Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

   Insert

   Trp53 (Trp53 Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 11, 2017

   Availability

   Academic Institutions and Nonprofits only

2. pJRK248

   Plasmid

   #173755

   Purpose

   co-injection plasmid

   Depositor

   Mike Boxem

   Article

   Kroll et al Genetics. 2021 Dec 10;219(4). pii: 63804

   Insert

   Prps-0::HygR::unc-54 3'UTR; Psqt-1::sqt-1::sqt-1 3' UTR
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMSCV-IRES-CFP II (pMIC II)

   Plasmid

   #52109

   Purpose

   a bicistronic IRES-CFP containing retroviral vector, MCS is expanded from pMSCV-IRES-GFP

   Depositor

   Dario Vignali

   Article

   Holst et al Nat Protoc. 2006;1(1):406-17.

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   IRES-ECFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pEGFPC1-hVAP-B

   Plasmid

   #104448

   Purpose

   Expression of human VAP-B fused to GFP in mammalian cells

   Depositor

   Catherine Tomasetto

   Article

   Alpy et al J Cell Sci. 2013 Dec 1;126(Pt 23):5500-1

   Insert

   vesicle-associated membrane protein-associated protein B (VAPB Human)
   

   Use

   Tags

   EGFP and HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pFCcGi

   Plasmid

   #59324

   Purpose

   Inducible expression of GFP and constitutive expression of mCherry

   Depositor

   David Holden, Sophie Helaine

   Article

   Figueira et al MBio. 2013 Apr 16;4(2):e00065. doi: 10.1

   Inserts

   mCherry
   EGFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Pbad and rpsM

   Available since

   Oct. 24, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. SPOP-V5 (BACK))

   Plasmid

   #169722

   Purpose

   Mammalian Expression of mouse SPOP Y353E

   Depositor

   Tanja Mittag

   Article

   Bouchard et al Mol Cell. 2018 Oct 4;72(1):19-36.e8. doi

   Insert

   SPOP (Spop Mouse)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Y353E

   Promoter

   CMV

   Available since

   June 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. SPOP-V5 (BTB)

   Plasmid

   #169721

   Purpose

   Mammalian Expression of mouse SPOP-BTB (L186D, L190D, L193D, I217K)

   Depositor

   Tanja Mittag

   Article

   Bouchard et al Mol Cell. 2018 Oct 4;72(1):19-36.e8. doi

   Insert

   SPOP (Spop Mouse)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   L186D, L190D, L193D, I217K

   Promoter

   CMV

   Available since

   June 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pPTn2P-PTK

   Plasmid

   #85604

   Purpose

   Passport transposon with a puromycin-thymidine kinase fusion driven by a PGK promoter.

   Depositor

   Scott Fahrenkrug, Karl Clark

   Article

   Clark et al BMC Biotechnol. 2007 Jul 17;7:42.

   Insert

   Passport transposon with a puromycin-thymidine kinase fusion
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   Dec. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDEW8

   Plasmid

   #84238

   Purpose

   Plasmid containing a P2A peptide (porcine teschovirus-1 derived) sequence that works efficiently in Saccharomyces cerevisiae

   Depositor

   David Weinberg

   Article

   DiSanto et al Elife. 2016 Oct 1;5. pii: e20069. doi: 1

   Insert

   HA-mRuby-P2A-eGFP
   

   Use

   Tags

   HA-mRuby and eGFP

   Expression

   Yeast

   Mutation

   Promoter

   GPD

   Available since

   Nov. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Topo_SpCas9.tracr_AsCas12a.DR

    Plasmid

    #155050

    Purpose

    TOPO vector for the cloning of _the SpCas9 tracrRNA - AsCas12a Direct Repeat (DR) fragment into the pLCHKO hgRNA vector

    Depositor

    Benjamin Blencowe, Jason Moffat

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    SpCas9 tracrRNA and AsCas12a Direct Repeat (DR)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBFv-nosP-Cas9

    Plasmid

    #138402

    Purpose

    Expresses Cas9 in the germline of Drosophila

    Depositor

    Shu Kondo

    Article

    Kondo et al Genetics. 2013 Nov;195(3):715-21. doi: 1

    Insert

    Cas9-NLS
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    UAS

    Available since

    March 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCE-KdGl

    Plasmid

    #154880

    Purpose

    Episomally expresses KDM4D and GLIS1 in mammalian cells

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al Stem Cell Reports. 2021 Mar 23. pii: S22

    Insert

    KDM4D, GLIS1 (KDM4D, GLIS1 Human, Callithrix jacchus)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. xGoosecoid

    Plasmid

    #24977

    Depositor

    Edward De Robertis

    Article

    Cho et al Cell. 1991 Dec 20. 67(6):1111-20.

    Insert

    Goosecoid (gsc-a Frog)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 29, 2010

    Availability

    Academic Institutions and Nonprofits only

14. pTipi2.1_Anti-DYKDDDDK [IPI-4E11/M1] Mouse/Rabbit HC

    Plasmid

    #221357

    Purpose

    Mammalian expression of Anti-DYKDDDDK [IPI-4E11/M1] Mouse/Rabbit HC; to be used with Anti-DYKDDDDK [IPI-4E11/M1] light chain (Plasmid 221358) to make the antibody.

    Depositor

    Institute for Protein Innovation (IPI)

    Insert

    Anti-DYKDDDDK [IPI-4E11/M1] Mouse/Rabbit HC
    

    Use

    Affinity Reagent/ Antibody

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 1, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pTipi2.1_Anti-DYKDDDDK [IPI-4E11/M1] Mouse/Rabbit LC kappa

    Plasmid

    #221358

    Purpose

    Mammalian expression of Anti-DYKDDDDK [IPI-4E11/M1] Mouse/Rabbit LC kappa; to be used with Anti-DYKDDDDK [IPI-4E11/M1] heavy chain (Plasmid 221357) to make the antibody.

    Depositor

    Institute for Protein Innovation (IPI)

    Insert

    Anti-DYKDDDDK [IPI-4E11/M1] Mouse/Rabbit LC kappa
    

    Use

    Affinity Reagent/ Antibody

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 1, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. CMV mRuby3-Synapsin1a

    Plasmid

    #187896

    Purpose

    For labeling pre-synaptic terminals

    Depositor

    Timothy Ryan

    Insert

    Synapsin1a
    

    Use

    Tags

    mRuby3

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. XP12 Xbra

    Plasmid

    #16978

    Depositor

    Randall Moon

    Insert

    Xbra (t.S Frog)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 1, 2008

    Availability

    Academic Institutions and Nonprofits only

18. pET30a-SARS-CoV2-NP

    Plasmid

    #165090

    Purpose

    protein expression in E. coli

    Depositor

    Rick Tarleton

    Article

    Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

    Insert

    Severe acute respiratory syndrome coronavirus 2 nucleocapsid protein N-terminal (N SARS-CoV2)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 10, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. FLAG-CDT2

    Plasmid

    #215091

    Purpose

    Expresses FLAG-CDT2 in mammalian cells.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    CDT2 (DTL Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. GFP-Rab27A

    Plasmid

    #89237

    Purpose

    Expression of GFP-tagged human Rab27A in mammalian cells

    Depositor

    William Gahl

    Article

    Westbroek et al Mol Genet Metab. 2008 Jun;94(2):248-54.

    Insert

    human Rab27A (RAB7A Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    CMV

    Available since

    April 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pHRIG-Akt1

    Plasmid

    #53583

    Purpose

    lentiviral expression of constitutively active human Akt1 and EGFP

    Depositor

    Heng Zhao

    Article

    Xie et al J Cereb Blood Flow Metab. 2013 Dec;33(12

    Insert

    cAkt1 (AKT1 Human)
    

    Use

    Lentiviral

    Tags

    HA and IRES-EGFP

    Expression

    Mammalian

    Mutation

    constitutively active (myristoylated version)

    Promoter

    CMV

    Available since

    Oct. 28, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pBHM2617

    Plasmid

    #234496

    Purpose

    NATR-CnU6-empty sgRNA

    Depositor

    Hiten Madhani

    Article

    Nalley et al bioRxiv 2025.02.14.638277

    Insert

    NAT-PCnU6-sgRNA_scaffold
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pMSP1D1-Strep-II

    Plasmid

    #224209

    Purpose

    Membrane scaffold protein (MSP1D1) with Strep-II tag. pMSP1D1, 7xHis tag swapped for a Strep-II tag.

    Depositor

    Anne Robinson

    Article

    Pettersen et al Biophys J. 2025 Jan 21;124(2):440-457. d

    Insert

    MSP1D1-Strep-II
    

    Use

    Tags

    Strep-II Tag

    Expression

    Bacterial

    Mutation

    Substitution of 7xHis tag found in pMSP1D1 for a …

    Promoter

    T7

    Available since

    July 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAAV.CAG.(cyto).iATPSnFR2.A95A.A119L.miRFP670nano3

    Plasmid

    #236406

    Purpose

    Expresses cytosolic low affinity ratiometric ATP sensor

    Depositor

    Timothy Ryan

    Article

    Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

    Insert

    (cyto).iATPSnFR2.A95A.A119L.miRFP670nano3
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA5-FRT/TO-APT2-LaG16-3W

    Plasmid

    #234425

    Purpose

    Express APT2 with a mutated non-binding anti-GFP nanobody LaG16 (F30W, T55W, G103W) fused at the C terminus separated by a flexible linker

    Depositor

    William Fuller

    Article

    Kuo et al Nat Commun. 2025 Feb 7;16(1):1445. doi:

    Insert

    APT2 fused to non-binding mutant LaG16 (LYPLA2 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA5-FRT/TO-APT1-LaG16-3W

    Plasmid

    #234424

    Purpose

    Express APT1 with a mutated non-binding anti-GFP nanobody LaG16 (F30W, T55W, G103W) fused at the C terminus separated by a flexible linker

    Depositor

    William Fuller

    Article

    Kuo et al Nat Commun. 2025 Feb 7;16(1):1445. doi:

    Insert

    APT1 fused to non-binding mutant LaG16 (LYPLA1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. AiP12493 - pAAV-AiE0374h-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2493)

    Plasmid

    #230214

    Purpose

    AiE0374h is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

    Depositor

    Boaz Levi, The Allen Institute for Brain Science

    Article

    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

    Insert

    SYFP2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    minBG

    Available since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. T2A 3xNLS GFP

    Plasmid

    #227721

    Purpose

    Plasmid contains T2A 3xNLS GFP, with some nlp-51 homology sequence before, Amp Resistant

    Depositor

    Oliver Hobert, Haosheng Sun

    Article

    Sun et al MicroPubl Biol. 2023 Nov 2;2023:10.17912

    Insert

    T2A 3xNLS GFP with some nlp-51 homology sequence (nlp-51 Nematode)
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pGEX-6P-acatenin

    Plasmid

    #186458

    Purpose

    Expresses human alpha-catenin with an N-terminal GST tag and a PRescission Protease cleavage site

    Depositor

    Tina Izard

    Article

    Rangarajan et al Commun Biol. 2023 Mar 16;6(1):276. doi:

    Insert

    CTNNA1 (CTNNA1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. DRH015_ AAV-hSyn-WT-THR

    Plasmid

    #225088

    Purpose

    Expression of wild-type thyroid receptor beta (THRB, human) with an HA tag in neurons driven by human synapsin promoter.

    Depositor

    Bernardo Sabatini

    Article

    Hochbaum et al Cell. 2024 Oct 3;187(20):5679-5697.e23.

    Insert

    WT-THRB (THRB Human)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pDRF1 mScarletI3-T2A-mTurquoise2

    Plasmid

    #219845

    Purpose

    equimolar expression of mScarletI3 and mTurquoise2 in budding yeast

    Depositor

    Bas Teusink

    Article

    Botman et al bioRxiv 2025.06.27.661975; doi: https://

    Insert

    mScarletI3-T2A-mTurquoise2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 27, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

32. pHIV-EGFP.Flag-EZH2-mt1

    Plasmid

    #220244

    Purpose

    Expresses an EZH2 mutant for knockout/rescue experiments in mammalian cells.

    Depositor

    Chen Davidovich

    Article

    Gail et al Nat Genet. 2024 May 14. doi: 10.1038/s41

    Insert

    EZH2 mt 1 (EZH2 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    R27A, R31A, F165A

    Promoter

    EF-1α

    Available since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pET28a(+)-SUMO-A'

    Plasmid

    #209115

    Purpose

    Bacterial expression of CC-GEMS ligand SUMO-A'

    Depositor

    Tom de Greef

    Article

    Makri Pistikou et al Nat Commun. 2023 Nov 2;14(1):7001. doi:

    Insert

    SUMO-A'
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. GpACR1-pcDNA3.1-mScarlet

    Plasmid

    #204964

    Purpose

    Expression of GpACR1 in fusion with mScarlet in mammalian cells

    Depositor

    Peter Hegemann

    Article

    Oppermann et al eLife. 2024 Oct 14;12:RP90100. doi: 10.7

    Insert

    GpACR1
    

    Use

    Tags

    mScarlet

    Expression

    Mammalian

    Mutation

    Promoter

    CMV (+ enhancer)

    Available since

    Oct. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. AiP12214 - pAAV-ProB12-SYFP2-WPRE3-BGHpA (Alias: CN2214)

    Plasmid

    #208161

    Purpose

    AAV vector for SYFP2 expression in brain astrocytes

    Depositor

    Jonathan Ting, The Allen Institute for Brain Science

    Article

    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

    Insert

    SYFP2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    NA

    Promoter

    Beta Globin minimal promoter

    Available since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pETM33_CLIP_CAP_Gly

    Plasmid

    #178470

    Purpose

    Bacterial expression of human domain with His-tag and GST tag

    Depositor

    Ylva Ivarsson

    Article

    Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

    Insert

    CLIP_CAP_Gly (CLIP1 Human, Synthetic)
    

    Use

    Tags

    His-GST

    Expression

    Bacterial

    Mutation

    Promoter

    T7/LacO

    Available since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pcDNA3-HA-DEPTOR S286/287/291A

    Plasmid

    #159160

    Purpose

    Expresses DEPTOR S286/287/291A mutant in mammalian cells

    Depositor

    Michele Pagano

    Article

    Duan et al Mol Cell. 2011 Oct 21;44(2):317-24. doi:

    Insert

    DEPTOR (DEPTOR Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    changed serine 286,287 and 291 to alanine

    Promoter

    CMV

    Available since

    Sept. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pcDNA3-HA-DEPTOR S286/287/291D

    Plasmid

    #159161

    Purpose

    Expresses DEPTOR S286/287/291D mutant in mammalian cells

    Depositor

    Michele Pagano

    Article

    Duan et al Mol Cell. 2011 Oct 21;44(2):317-24. doi:

    Insert

    DEPTOR (DEPTOR Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    changed serine 286,287 and 291 to aspartic acid

    Promoter

    CMV

    Available since

    Sept. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. p206_LTJ_sgRNACD45.2_R3

    Plasmid

    #82674

    Purpose

    sgRNA targeting murine CD45.2 region 3. Co-expresses SpCas9-2A-EGFP.

    Depositor

    Lukas Jeker

    Article

    Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

    Insert

    sgRNA targeting CD45.2 region 3
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pHCMV_RVFV_GP

    Plasmid

    #207304

    Purpose

    Mammalian expression of Rift valley fever virus GP protein

    Depositor

    Feng Zhang

    Article

    Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

    Insert

    RVFV_GP (RVFV_sM_gp1 Rift valley fever virus)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    mammalian codon optimization

    Promoter

    CMV promoter

    Available since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge