Skip to main content
Addgene
Showing: 1001 - 1050 of 2174 results

  11. pAAV-EGFP.T2A.NCLX

    Plasmid
    #181873
    Purpose
    Expresses EGFP and murine NCLX (separated by a T2A cleavage site) under control of the synthetic CAG promoter
    Depositor
    Hilmar Bading
    Article
    Hagenston et al J Biol Chem. 2021 Dec 20:101508. doi: 10
    Inserts
    solute carrier family 8 member B1 (Slc8a1 Mouse)
    EGFP
    Use
    AAV and Adenoviral
    Tags
    HA and myc
    Expression
    Mammalian
    Mutation
    Promoter
    synthetic hybrid CAG promoter and synthetic hybri…
    Available since
    March 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    181873-plasmid-map-sequence-id-355952

    Enlarge

  12. Lenti_EGFP-P2A_truncHaus6_IRES_Blast

    Plasmid
    #182885
    Purpose
    Transfer vector for production of lentivirus. Control plasmid for rescue experiment of HAUS6 depletion phenotype, containing a nonsense mutation and a truncation in HAUS6.
    Depositor
    Daniel Gerlich
    Article
    David et al J Cell Biol. 2019 Jul 1;218(7):2150-2168
    Insert
    HAUS 6 (HAUS6 Human)
    Use
    Lentiviral
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    stop codon introduced 2 amino acids downstream of…
    Promoter
    EF1alpha core
    Available since
    July 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    182885-plasmid-map-sequence-id-367930

    Enlarge

  16. pET29b-AviTag-mEGFP-dspB(E184Q W330Y)-6xHis

    Plasmid
    #176575
    Purpose
    Plasmid for overexpression of recombinant AviTagged, His-tagged fusion protein mEGFP-DspB(E184Q W330Y), used as a non-binding control for detection of biofilm polysaccharide PNAG.
    Depositor
    Mark Nitz
    Article
    Eddenden et al Methods Enzymol. 2022;665:209-231. doi:
    Insert
    Dispersin B
    Use
    Tags
    Avitag, Hexahistidine tag, and mEGFP
    Expression
    Bacterial
    Mutation
    E184Q W330Y
    Promoter
    T7
    Available since
    Nov. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    176575-plasmid-map-sequence-id-348273

    Enlarge

  19. p415 TEF roGFP2‐Tsa2ΔCPΔCR

    Plasmid
    #83239
    Purpose
    The catalytically inactive genetically encoded fluorescent H2O2 sensor roGFP2-Tsa2ΔCPΔCR cloned in the yeast p415 vector under the control of a TEF promoter. For cytosolic expression.
    Depositor
    Tobias Dick
    Article
    Morgan et al Nat Chem Biol. 2016 Jun;12(6):437-43. do
    Insert
    roGFP2-Tsa2dCpdCr
    Use
    Tags
    roGFP2 fusion with Tsa2dCpdCr
    Expression
    Yeast
    Mutation
    Promoter
    TEF
    Available since
    Sept. 30, 2016
    Availability
    Academic Institutions and Nonprofits only
    83239-plasmid-map-sequence-id-158262

    Enlarge

  27. pAAV_hSyn_NES-Kinprola_on-mEGFP_WRPE-SV40

    Plasmid
    #233368
    Purpose
    hSyn1 driven expression of the consitutively active kinase activity recorder positive control Kinprola_on fused to mEGFP for neuronal expression through AAV transduction
    Depositor
    Kai Johnsson
    Article
    Sun et al bioRxiv 2024.09.11.611894
    Insert
    NES-Kinprola_on-mEGFP
    Use
    AAV
    Tags
    NES and mEGFP
    Expression
    Mutation
    Promoter
    hSyn1
    Available since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    233368-plasmid-map-sequence-id-466650

    Enlarge

  28. pAAV_hSyn_NES-Kinprola_PKA_T/A-mEGFP_WRPE-SV40

    Plasmid
    #233367
    Purpose
    hSyn1 driven expression of the inactive PKA activity recorder negative control with T/A mutation Kinprola_PKA fused to mEGFP for neuronal expression through AAV transduction
    Depositor
    Kai Johnsson
    Article
    Sun et al bioRxiv 2024.09.11.611894
    Insert
    NES-Kinprola_PKA_T/A-mEGFP
    Use
    AAV
    Tags
    NES and mEGFP
    Expression
    Mutation
    Promoter
    hSyn1
    Available since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    233367-plasmid-map-sequence-id-466644

    Enlarge

  32. pCDNA5/FRT_CMV_NES-Kinprola_on-mEGFP

    Plasmid
    #233355
    Purpose
    CMV driven expression of the consitutively active kinase activity recorder positive control Kinprola_on in mammalian cell lines fused to mEGFP
    Depositor
    Kai Johnsson
    Article
    Sun et al bioRxiv 2024.09.11.611894
    Insert
    NES-Kinprola_on-mEGFP
    Use
    Tags
    NES and mEGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    233355-plasmid-map-sequence-id-466631

    Enlarge

  34. pLKO.1-puro_CMV_NES-Kinprola_on-mEGFP

    Plasmid
    #233370
    Purpose
    CMV driven expression of the consitutively active kinase activity recorder positive control Kinprola_on fused to mEGFP for neuronal expression through lentivirus transduction
    Depositor
    Kai Johnsson
    Article
    Sun et al bioRxiv 2024.09.11.611894
    Insert
    NES-Kinprola_on-mEGFP
    Use
    Lentiviral
    Tags
    NES and mEGFP
    Expression
    Mutation
    Promoter
    CMV
    Available since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    233370-plasmid-map-sequence-id-466616

    Enlarge

  38. SCN1Aprom(335-406)-H2B-GFP

    Plasmid
    #236151
    Purpose
    Fluorescent reporter encoding H2B-GFP fusion under control of E1b minimal promoter with the SCN1a promoter region encompassing 335 to 406 bp upstream of its transcriptional start site
    Depositor
    Xiaojing Gao
    Article
    Wolfsberg et al bioRxiv 2025.02.24.639918
    Insert
    H2B (H2BC11 Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    E1b minimal promoter with the SCN1a promoter regi…
    Available since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    236151-plasmid-map-sequence-id-468384

    Enlarge

  39. FUW-tetO-EGFP

    Plasmid
    #84041
    Purpose
    3rd generation lentiviral transfer plasmid. Expresses EGFP and puromycin resistance in mammalian cells under the control of a doxycycline-inducible promoter
    Depositor
    Stefano Piccolo
    Article
    Panciera et al Cell Stem Cell. 2016 Sep 9. pii: S1934-5
    Insert
    Enhanced Green Fluorescence Protein, Puromycin resistance gene
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE-CMV
    Available since
    Oct. 31, 2016
    Availability
    Academic Institutions and Nonprofits only
    84041-plasmid-map-sequence-id-164306

    Enlarge

  43. pMuLE Lenti Dest eGFP

    Plasmid
    #62175
    Purpose
    MuLE (Multiple Lentiviral Expression) Destination vector for use with pMuLE Entry vectors. Co-expresses EGFP under the control of a PGK promoter. Compatible with MultiSite Gateway cloning
    Depositor
    Ian Frew
    Article
    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d
    Type
    Empty backbone
    Use
    Lentiviral; Mule gateway destination vector
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    62175-plasmid-map-sequence-id-347572

    Enlarge

Showing: 1001 - 1050 of 2174 results