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We narrowed to 13,971 results for: EGFP

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Showing: 10701 - 10750 of 13971 results

  3. pNLS-2xBFP(4C)

    Plasmid
    #176151
    Purpose
    Encodes NLS-2xBFP(4C), which is a tandem BFP with an N-terminal SV40 NLS. The protein has 4 solvent accessible cysteines for labeling with maleimide dyes.
    Depositor
    Siegfried Musser
    Article
    Chowdhury et al Nat Cell Biol. 2022 Jan;24(1):112-122. d
    Insert
    NLS-2xBFP(4C)
    Tags
    6xHis tag and SV40 nuclear localization sequence …
    Expression
    Bacterial
    Mutation
    Compared with EGFP, the two BFP domains have the …
    Promoter
    Ptrc
    Available Since
    March 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    176151-plasmid-map-sequence-id-356068

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  16. PB-CAG-DDdCas9VP192-T2A-GFP-IRES-Neo

    Plasmid
    #102885
    Purpose
    PiggyBac transposon system construct with constitutive CAG promoter expression of TMP inducible DDdCas9VP192 activator followed by T2A-EGFP as a reporter and IRES-Neo as selection
    Depositor
    Timo Otonkoski
    Article
    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1
    Insert
    DDdCas9VP192-T2A-GFP-IRES-Neo
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Promoter
    CAG
    Available Since
    July 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    102885-plasmid-map-sequence-id-259794

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  23. pLenti-PGK-c-myc-2A-CDK4(R24C)-GFP-Puro

    Plasmid
    #83107
    Purpose
    3rd generation lentiviral expression of C-myc, CDK4(R24C), and GFP separated by 2A self-cleaving peptide
    Depositor
    Andrew Brooks
    Inserts
    c-Myc (MYC Human)
    CDK4 (CDK4 Human)
    eGFP
    Use
    Lentiviral
    Tags
    2A self cleaving peptide
    Expression
    Mammalian
    Mutation
    R24C and p64 isoform
    Promoter
    hPGK and hPGK (single transcript)
    Available Since
    July 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    83107-plasmid-map-sequence-id-330062

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  24. pTK331

    Plasmid
    #59572
    Purpose
    TC-83 I-SceI T7 5'UTR (A3G) P1234 (nsP2 Q739L) SGP(14) Kozak mKate-G8-L7Ae-PEST 4xFF4 attB2 SGP2(98/30) XbaI attB1 EBFP2 ochre attB2 (insert) AscI (truncated E1) 3'UTR Poly A I-SceI
    Depositor
    Ron Weiss
    Article
    Wroblewska et al Nat Biotechnol. 2015 Aug 3. doi: 10.1038
    Insert
    SGP(16) 2xK-turn Kozak EGFP attB2 AscI
    Available Since
    Aug. 3, 2015
    Availability
    Academic Institutions and Nonprofits only
    59572-plasmid-map-sequence-id-92977

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  25. 908H

    Plasmid
    #160240
    Purpose
    TetO(2x)-Hsp70min-UASpmin-Firefly-T2A-eGFP-p10-3’UTR-Ubiquitin-Renilla-attB
    Depositor
    Omar Akbari
    Article
    Gamez et al Insect Mol Biol. 2021 Oct;30(5):461-471.
    Inserts
    Tet Operator (2x)
    hsp70 minimal promoter
    p-element promoter
    Firefly luciferase
    Renilla luciferase
    Ubiquitin promoter
    Tags
    T2A GFP
    Expression
    Insect
    Available Since
    Nov. 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    160240-plasmid-map-sequence-id-316017

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  28. Nrxn3α-AS4-

    Plasmid
    #184332
    Purpose
    Expression of mouse Nrx3-alpha lacking insertion at the alternative splice site 4 together with EGFP protein separated by 2A peptide. Contains insertions at alternatively splice sites 1,2,3 and 6.
    Depositor
    Peter Scheiffele
    Article
    Nguyen et al Elife. 2016 Dec 13;5. doi: 10.7554/eLife
    Insert
    Nrxn3-alpha (Nrxn3 Mouse)
    Tags
    HA-tag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    184332-plasmid-map-sequence-id-361409

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  30. ZJOM10

    Plasmid
    #133639
    Purpose
    Integration of VPS4-GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Marker for yeast late endosome.
    Depositor
    Zhiping Xie
    Article
    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-
    Insert
    p1k-Vps4-GFP (VPS4 Budding Yeast)
    Tags
    EGFP
    Expression
    Yeast
    Available Since
    Feb. 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    133639-plasmid-map-sequence-id-265901

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  33. CIBN-GFP-TMD

    Plasmid
    #79571
    Purpose
    expression of CIBN domain fused to GFP tagged mouse Syntaxin1 TMD domain (transmembrane domain and polybasic region)
    Depositor
    Pietro De Camilli, Olof Idevall-Hagren
    Article
    Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10
    Insert
    syntaxin 1A (Stx1a Mouse)
    Tags
    CIBN and EGFP
    Expression
    Mammalian
    Mutation
    aa 249_288 (C-terminal region (polybasic stretch …
    Promoter
    CMV
    Available Since
    July 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    79571-plasmid-map-sequence-id-456519

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  35. MSCV-myrAkt

    Plasmid
    #49267
    Purpose
    retroviral expression of Bovine Akt with the NH2-terminal myristylation-palmitylation signal sequence
    Depositor
    Lawrence Kane, Arthur Weiss
    Article
    Kane et al Nat Immunol. 2001 Jan;2(1):37-44.
    Insert
    Akt (AKT1 Bovine)
    Use
    Retroviral
    Tags
    IRES-EGFP and myr
    Expression
    Mammalian
    Mutation
    N2D, D149E and A478G compared to AAW71957.1
    Available Since
    Jan. 21, 2014
    Availability
    Academic Institutions and Nonprofits only

  42. Nrxn3α-AS4+

    Plasmid
    #184333
    Purpose
    Expression of mouse Nrx3-alpha containing insertion at the alternative splice site 4 together with EGFP protein separated by 2A peptide. Contains insertions at alternatively splice sites 1,2,3 and 6.
    Depositor
    Peter Scheiffele
    Article
    Nguyen et al Elife. 2016 Dec 13;5. doi: 10.7554/eLife
    Insert
    Nrxn3-alpha (Nrxn3 Mouse)
    Tags
    HA-tag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    184333-plasmid-map-sequence-id-361408

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  47. pBiex PKC - Peptide 8 GFP RFP

    Plasmid
    #83564
    Purpose
    Expresses a GFP-RFP FRET sensor containing the catalytic domain of PKCalpha and a short peptide substrate derived from the EGF receptor.
    Depositor
    Sivaraj Sivaramakrishnan
    Article
    Sommese et al J Biol Chem. 2016 Aug 23. pii: jbc.M116.
    Insert
    PKC alpha (PRKCA Human)
    Tags
    10 nm ER/K Helix, FLAG purification tag, TEV Prot…
    Expression
    Bacterial and Insect
    Mutation
    Only catalytic domain of PKC (aa 335-672)
    Promoter
    T7 Promoter
    Available Since
    Nov. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    83564-plasmid-map-sequence-id-166406

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Showing: 10701 - 10750 of 13971 results