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Showing: 10701 - 10750 of 23409 results

  1. pMMACE DEST CMV SpCas9

    Plasmid
    #206268
    Purpose
    DEST vector for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes SpCas9 under the control of CMV promoter. CRE-Acceptor in MultiBac system.
    Depositor
    Imre Berger
    Article
    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77
    Insert
    SpCas9
    Use
    CRISPR; Recombinant baculovirus production
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    206268-plasmid-map-sequence-id-406404

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  3. p426_Cas9_gRNA-ARS1114a

    Plasmid
    #87397
    Purpose
    All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting ARS1114a sequence CTTGTGAAACAAATAATTGG in yeast chromosome 11.
    Depositor
    Aindrila Mukhopadhyay
    Article
    Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-
    Insert
    Human Optimized S. pyogenes Cas9 and guide RNA targeting ARS1114a
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    ADH1, pTyrosine
    Available since
    April 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    87397-plasmid-map-sequence-id-193440

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  4. pLY152

    Plasmid
    #130950
    Purpose
    dcas9 generator and reporter circuit (PpspA-2G6 with sfgfp::ASV)
    Depositor
    Baojun Wang
    Article
    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:
    Inserts
    dcas9
    tetR
    sfgfp
    Use
    Synthetic Biology
    Tags
    ASV tag
    Expression
    Bacterial
    Mutation
    Mutations D10A and H840A on WT cas9. Synonymous m…
    Promoter
    PpspA-2G6 and Ptet
    Available since
    Sept. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    130950-plasmid-map-sequence-id-257362

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  5. qTAG-N-Blast-sTagRFP-TUBA1B

    Plasmid
    #207768
    Purpose
    Donor template for Blast-2A-sTagRFP insertion into the N-terminus of the TUBA1B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B Addgene #207763
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    TUBA1B Homology Arms flanking a Blast-sTagRFP Cassette (TUBA1B Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 15, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    207768-plasmid-map-sequence-id-413803

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  6. p426_Cas9_gRNA-ARS308a

    Plasmid
    #87384
    Purpose
    All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting ARS308a sequence CACTTGTCAAACAGAATATA in yeast chromosome 3
    Depositor
    Aindrila Mukhopadhyay
    Article
    Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-
    Insert
    Human Optimized S. pyogenes Cas9 and guide RNA targeting ARS308a
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    ADH1, pTyrosine
    Available since
    April 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    87384-plasmid-map-sequence-id-193426

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  9. pLKO_AU1.HA.VSVg_mCherry-NLS

    Plasmid
    #178220
    Purpose
    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.
    Depositor
    Brian Brown
    Article
    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00
    Insert
    nPC Tagged mCherry-NLS
    Use
    Lentiviral
    Tags
    AU1.HA.VSVg and Nuclear Localization Signal
    Expression
    Mutation
    Promoter
    eF1a
    Available since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    178220-plasmid-map-sequence-id-352764

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  10. pLKO_AU1.HA.S_mCherry-NLS

    Plasmid
    #178219
    Purpose
    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.
    Depositor
    Brian Brown
    Article
    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00
    Insert
    nPC Tagged mCherry-NLS
    Use
    Lentiviral
    Tags
    AU1.HA.S and Nuclear Localization Signal
    Expression
    Mutation
    Promoter
    eF1a
    Available since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    178219-plasmid-map-sequence-id-352763

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  11. pLKO_AU1.HA.HSV_mCherry-NLS

    Plasmid
    #178216
    Purpose
    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.
    Depositor
    Brian Brown
    Article
    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00
    Insert
    nPC Tagged mCherry-NLS
    Use
    Lentiviral
    Tags
    AU1.HA.HSV and Nuclear Localization Signal
    Expression
    Mutation
    Promoter
    eF1a
    Available since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    178216-plasmid-map-sequence-id-352760

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  12. pLKO_AU1.C.VSVg_mCherry-NLS

    Plasmid
    #178214
    Purpose
    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.
    Depositor
    Brian Brown
    Article
    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00
    Insert
    nPC Tagged mCherry-NLS
    Use
    Lentiviral
    Tags
    AU1.C.VSVg and Nuclear Localization Signal
    Expression
    Mutation
    Promoter
    eF1a
    Available since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    178214-plasmid-map-sequence-id-352758

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  13. pLKO_AU1.C.S_mCherry-NLS

    Plasmid
    #178213
    Purpose
    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.
    Depositor
    Brian Brown
    Article
    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00
    Insert
    nPC Tagged mCherry-NLS
    Use
    Lentiviral
    Tags
    AU1.C.S and Nuclear Localization Signal
    Expression
    Mutation
    Promoter
    eF1a
    Available since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    178213-plasmid-map-sequence-id-352757

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  14. pLKO_AU1.C.HSV_mCherry-NLS

    Plasmid
    #178210
    Purpose
    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.
    Depositor
    Brian Brown
    Article
    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00
    Insert
    nPC Tagged mCherry-NLS
    Use
    Lentiviral
    Tags
    AU1.C.HSV and Nuclear Localization Signal
    Expression
    Mutation
    Promoter
    eF1a
    Available since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    178210-plasmid-map-sequence-id-352753

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  16. p426_Cas9_gRNA-ARS511b

    Plasmid
    #87387
    Purpose
    All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting ARS511b sequence CAGTGTATGCCAGTCAGCCA in yeast chromosome 5.
    Depositor
    Aindrila Mukhopadhyay
    Article
    Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-
    Insert
    Human Optimized S. pyogenes Cas9 and guide RNA targeting ARS511b
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    ADH1, pTyrosine
    Available since
    April 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    87387-plasmid-map-sequence-id-193429

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  18. pLKO_NWS.Ollas.S_mCherry-NLS

    Plasmid
    #178279
    Purpose
    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.
    Depositor
    Brian Brown
    Article
    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00
    Insert
    nPC Tagged mCherry-NLS
    Use
    Lentiviral
    Tags
    NWS.Ollas.S and Nuclear Localization Signal
    Expression
    Mutation
    Promoter
    eF1a
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    178279-plasmid-map-sequence-id-353143

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  19. pLKO_HSV.S.V5_mCherry-NLS

    Plasmid
    #178278
    Purpose
    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.
    Depositor
    Brian Brown
    Article
    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00
    Insert
    nPC Tagged mCherry-NLS
    Use
    Lentiviral
    Tags
    HSV.S.V5 and Nuclear Localization Signal
    Expression
    Mutation
    Promoter
    eF1a
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    178278-plasmid-map-sequence-id-353142

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  20. pLKO_HSV.Ollas.V5_mCherry-NLS

    Plasmid
    #178277
    Purpose
    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.
    Depositor
    Brian Brown
    Article
    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00
    Insert
    nPC Tagged mCherry-NLS
    Use
    Lentiviral
    Tags
    HSV.Ollas.V5 and Nuclear Localization Signal
    Expression
    Mutation
    Promoter
    eF1a
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    178277-plasmid-map-sequence-id-353139

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  21. pLKO_HSV.NWS.S_mCherry-NLS

    Plasmid
    #178273
    Purpose
    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.
    Depositor
    Brian Brown
    Article
    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00
    Insert
    nPC Tagged mCherry-NLS
    Use
    Lentiviral
    Tags
    HSV.NWS.S and Nuclear Localization Signal
    Expression
    Mutation
    Promoter
    eF1a
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    178273-plasmid-map-sequence-id-353133

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  22. p426_Cas9_gRNA-ARS1309a

    Plasmid
    #87399
    Purpose
    All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting ARS1309a sequence CCTGTGGTGACTACGTATCC in yeast chromosome 13.
    Depositor
    Aindrila Mukhopadhyay
    Article
    Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-
    Insert
    Human Optimized S. pyogenes Cas9 and guide RNA targeting ARS1309a
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    ADH1, pTyrosine
    Available since
    April 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    87399-plasmid-map-sequence-id-193442

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  24. p426_Cas9_gRNA-HIS3b

    Plasmid
    #87402
    Purpose
    All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting HIS3b sequence AATATAGAGTGTACTAGAGG in yeast chromosome 15.
    Depositor
    Aindrila Mukhopadhyay
    Article
    Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-
    Insert
    Human Optimized S. pyogenes Cas9 and guide RNA targeting HIS3b
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    ADH1, pTyrosine
    Available since
    April 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    87402-plasmid-map-sequence-id-193445

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  25. Pdpy-30-miniCAFE

    Plasmid
    #170115
    Purpose
    Dpy-30 promoter-driven expression of a truncated VPR-dCjCas9 fusion protein (MiniCAFE) for activation of gene expression.
    Depositor
    Zhili Rong
    Article
    Zhang et al Nucleic Acids Res. 2021 Mar 22. pii: 617
    Insert
    MiniCAFE
    Use
    CRISPR
    Tags
    Expression
    Mutation
    D8A, HNH-truncation (Δ495–609 aa) in CjCas9
    Promoter
    Dpy-30
    Available since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    170115-plasmid-map-sequence-id-336055

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  27. p426_Cas9_gRNA-ARS1622b

    Plasmid
    #87403
    Purpose
    All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting ARS1622b sequence GTCACGTTCCTGAGGTTACT in yeast chromosome 16.
    Depositor
    Aindrila Mukhopadhyay
    Article
    Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-
    Insert
    Human Optimized S. pyogenes Cas9 and guide RNA targeting ARS1622b
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    ADH1, pTyrosine
    Available since
    April 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    87403-plasmid-map-sequence-id-193446

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  30. PB-PE VQR

    Plasmid
    #162797
    Purpose
    All-in-one prime editor piggyBac transposon, VQR variant
    Depositor
    Tobias Cantz
    Article
    Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1
    Insert
    SpCas9_H840A_VQR-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
    Use
    CRISPR and Synthetic Biology; Piggybac transposon
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    D1135V, R1335Q, T1337R
    Promoter
    CAG
    Available since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    162797-plasmid-map-sequence-id-320036

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  32. pLKO_HA.S.VSVg_mCherry-NLS

    Plasmid
    #178269
    Purpose
    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.
    Depositor
    Brian Brown
    Article
    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00
    Insert
    nPC Tagged mCherry-NLS
    Use
    Lentiviral
    Tags
    HA.S.VSVg and Nuclear Localization Signal
    Expression
    Mutation
    Promoter
    eF1a
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    178269-plasmid-map-sequence-id-353129

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  33. pLKO_HA.HSV.VSVg_mCherry-NLS

    Plasmid
    #178261
    Purpose
    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.
    Depositor
    Brian Brown
    Article
    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00
    Insert
    nPC Tagged mCherry-NLS
    Use
    Lentiviral
    Tags
    HA.HSV.VSVg and Nuclear Localization Signal
    Expression
    Mutation
    Promoter
    eF1a
    Available since
    Jan. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    178261-plasmid-map-sequence-id-353016

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  34. pLKO_HA.HSV.S_mCherry-NLS

    Plasmid
    #178260
    Purpose
    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.
    Depositor
    Brian Brown
    Article
    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00
    Insert
    nPC Tagged mCherry-NLS
    Use
    Lentiviral
    Tags
    HA.HSV.S and Nuclear Localization Signal
    Expression
    Mutation
    Promoter
    eF1a
    Available since
    Jan. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    178260-plasmid-map-sequence-id-353014

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  35. pLKO_C.S.VSVg_mCherry-NLS

    Plasmid
    #178255
    Purpose
    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.
    Depositor
    Brian Brown
    Article
    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00
    Insert
    nPC Tagged mCherry-NLS
    Use
    Lentiviral
    Tags
    C.S.VSVg and Nuclear Localization Signal
    Expression
    Mutation
    Promoter
    eF1a
    Available since
    Jan. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    178255-plasmid-map-sequence-id-353006

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  36. pLKO_C.HSV.VSVg_mCherry-NLS

    Plasmid
    #178246
    Purpose
    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.
    Depositor
    Brian Brown
    Article
    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00
    Insert
    nPC Tagged mCherry-NLS
    Use
    Lentiviral
    Tags
    C.HSV.VSVg and Nuclear Localization Signal
    Expression
    Mutation
    Promoter
    eF1a
    Available since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    178246-plasmid-map-sequence-id-352849

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  37. pLKO_C.HSV.S_mCherry-NLS

    Plasmid
    #178245
    Purpose
    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.
    Depositor
    Brian Brown
    Article
    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00
    Insert
    nPC Tagged mCherry-NLS
    Use
    Lentiviral
    Tags
    C.HSV.S and Nuclear Localization Signal
    Expression
    Mutation
    Promoter
    eF1a
    Available since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    178245-plasmid-map-sequence-id-352848

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  38. pLKO_C.HA.VSVg_mCherry-NLS

    Plasmid
    #178241
    Purpose
    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.
    Depositor
    Brian Brown
    Article
    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00
    Insert
    nPC Tagged mCherry-NLS
    Use
    Lentiviral
    Tags
    C.HA.VSVg and Nuclear Localization Signal
    Expression
    Mutation
    Promoter
    eF1a
    Available since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    178241-plasmid-map-sequence-id-352841

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  39. pLKO_C.HA.S_mCherry-NLS

    Plasmid
    #178240
    Purpose
    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.
    Depositor
    Brian Brown
    Article
    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00
    Insert
    nPC Tagged mCherry-NLS
    Use
    Lentiviral
    Tags
    C.HA.S and Nuclear Localization Signal
    Expression
    Mutation
    Promoter
    eF1a
    Available since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    178240-plasmid-map-sequence-id-352840

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  40. pLKO_C.HA.HSV_mCherry-NLS

    Plasmid
    #178237
    Purpose
    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.
    Depositor
    Brian Brown
    Article
    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00
    Insert
    nPC Tagged mCherry-NLS
    Use
    Lentiviral
    Tags
    C.HA.HSV and Nuclear Localization Signal
    Expression
    Mutation
    Promoter
    eF1a
    Available since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    178237-plasmid-map-sequence-id-352835

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  41. pLKO_AU1.HSV.VSVg_mCherry-NLS

    Plasmid
    #178225
    Purpose
    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.
    Depositor
    Brian Brown
    Article
    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00
    Insert
    nPC Tagged mCherry-NLS
    Use
    Lentiviral
    Tags
    AU1.HSV.VSVg and Nuclear Localization Signal
    Expression
    Mutation
    Promoter
    eF1a
    Available since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    178225-plasmid-map-sequence-id-352775

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  42. p426_Cas9_gRNA-ARS911b

    Plasmid
    #87394
    Purpose
    All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting ARS911b sequence GTAATATTGTCTTGTTTCCC in yeast chromosome 9.
    Depositor
    Aindrila Mukhopadhyay
    Article
    Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-
    Insert
    Human Optimized S. pyogenes Cas9 and guide RNA targeting ARS911b
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    ADH1, pTyrosine
    Available since
    April 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    87394-plasmid-map-sequence-id-193437

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  45. pAAV_ACTB CRISPIE

    Plasmid
    #172853
    Purpose
    Actb sgRNA & DRS-2 sgRNA expression under U6. CRISPIE donor mEGFP phase (0-0), excised by DRS-1 or DRS-2 (with SpCas9). Also expresses mRuby3 under the CBA promotor. See Zhong et al, eLife 2021.
    Depositor
    Haining Zhong
    Article
    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1
    Inserts
    Actb intron 1 sgRNA
    DRS-2 sgRNA
    CRISPIE designer exon (phase 0-0) encoding mEGFP
    mRuby3
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    172853-plasmid-map-sequence-id-341002

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  46. pLKO_AU1.HSV.Ollas_mCherry-NLS

    Plasmid
    #178223
    Purpose
    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.
    Depositor
    Brian Brown
    Article
    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00
    Insert
    nPC Tagged mCherry-NLS
    Use
    Lentiviral
    Tags
    AU1.HSV.Ollas and Nuclear Localization Signal
    Expression
    Mutation
    Promoter
    eF1a
    Available since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    178223-plasmid-map-sequence-id-352772

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  47. pLKO_AU1.HA.V5_mCherry-NLS

    Plasmid
    #178221
    Purpose
    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.
    Depositor
    Brian Brown
    Article
    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00
    Insert
    nPC Tagged mCherry-NLS
    Use
    Lentiviral
    Tags
    AU1.HA.V5 and Nuclear Localization Signal
    Expression
    Mutation
    Promoter
    eF1a
    Available since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    178221-plasmid-map-sequence-id-352765

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  48. pLKO_AU1.HA.Ollas_mCherry-NLS

    Plasmid
    #178218
    Purpose
    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.
    Depositor
    Brian Brown
    Article
    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00
    Insert
    nPC Tagged mCherry-NLS
    Use
    Lentiviral
    Tags
    AU1.HA.Ollas and Nuclear Localization Signal
    Expression
    Mutation
    Promoter
    eF1a
    Available since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    178218-plasmid-map-sequence-id-352762

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  49. pLKO_AU1.C.V5_mCherry-NLS

    Plasmid
    #178215
    Purpose
    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.
    Depositor
    Brian Brown
    Article
    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00
    Insert
    nPC Tagged mCherry-NLS
    Use
    Lentiviral
    Tags
    AU1.C.V5 and Nuclear Localization Signal
    Expression
    Mutation
    Promoter
    eF1a
    Available since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    178215-plasmid-map-sequence-id-352759

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  50. pLKO_AU1.C.Ollas_mCherry-NLS

    Plasmid
    #178212
    Purpose
    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.
    Depositor
    Brian Brown
    Article
    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00
    Insert
    nPC Tagged mCherry-NLS
    Use
    Lentiviral
    Tags
    AU1.C.Ollas and Nuclear Localization Signal
    Expression
    Mutation
    Promoter
    eF1a
    Available since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    178212-plasmid-map-sequence-id-352756

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Showing: 10701 - 10750 of 23409 results