19,167 results
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Plasmid#85181PurposeConstitutively active NFAT1, mutated to interfere selectively with the NFAT:AP-1 interaction. Co-expresses EGFP for selectionDepositorInsertCA-RIT-NFAT1 (Nfatc2 Mouse)
UseRetroviralTagsHAExpressionMammalianMutationAmino acids 1-3 removed; CA: PASSGSSASF mutated t…PromoterAvailable sinceApril 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAGFP
Plasmid#32748DepositorInsertGFP
UseTagsDEVDFQGPCNDSSDPLVVAASIIGILHLILWILDRLExpressionMammalianMutationS65TPromoterCMVAvailable sinceMarch 5, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLEX307-SARS-CoV-2-3CL
Plasmid#160278PurposeAllows for constituitive mammalian expression of the SARS-CoV-2 3CL protease via transfection or lentivirusDepositorInsert3CL Protease
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceOct. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pSFFV_sfCherry2_TagBFP
Plasmid#83031PurposeExpresses full length sfCherry2 and TagBFP fusion in mammalian cellsDepositorInsertsfCherry+full length_TagBFP
UseLentiviralTagsExpressionMammalianMutation3 mutations in sfCherry full length: E118Q, T128I…PromoterSFFVAvailable sinceJan. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
Tet-O-FUW-Neurod1
Plasmid#30129DepositorInsertNeuroD1 (Neurod1 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
pTA7002-avrPto
Plasmid#49156PurposePlant transformation binary vector for Dexamethasone inducible expression of the type III effector gene avrPtoDepositorInsertavrPto
UsePlant transformation binary vectorTagsExpressionMutationPromoterdexamethasone inducibleAvailable sinceApril 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET-Hisx6-rAPOBEC1-XTEN-nCas9-UGI-NLS
Plasmid#89508PurposeExpression of Hisx6-rAPOBEC1-XTEN-nCas9-UGI-NLS in bacterial cells.DepositorInsertrAPOBEC1-nCas9 (D10A)-UGI-NLS
UseTagsUGI and rAPOBEC-XTENExpressionBacterialMutationD10A mutation of Cas9PromoterT7Available sinceMay 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
phage UbiC NLS HA stdMCP Halo
Plasmid#104098PurposeLentiviral vector expressing synonymous tandem MS2 coat protein fused to Halo. Used to label reporter mRNAs.DepositorInsertstdMCP-Halo
UseLentiviralTagsC-terminal Halo-tag, N-terminal NLS, and Two syno…ExpressionMammalianMutationPromoterAvailable sinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-NTRK1
Plasmid#23891DepositorInsertNTRK1 (NTRK1 Human)
UseGateway donor vectorTagsExpressionMutationV263L relative to NM_001012331PromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
PCS2-FlipGFP(Casp3 cleavage seq)
Plasmid#124432PurposeExpresses FlipGFP (caspase-3 cleavage sequence) protease reporter in zebrafish (verified)DepositorInsertFlipGFP(Casp3 cleavage seq)
UseZebrafish (good expression)TagsExpressionMutationPromoterAvailable sinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Lyn-ICUE3
Plasmid#61623PurposePlasma membrane-targeted FRET biosensor for monitoring cAMPDepositorInsertLyn-ICUE3 (RAPGEF3 Human)
UseTagsECFP, N-terminal targeting sequence from Lyn kina…ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCS2+MCS-P2A-sfGFP
Plasmid#74668PurposePlasmid for expression of wild-type or mutant cDNA fragments (mutation reporter) or complete cDNAs in frame with superfolderGFP.DepositorInsertsfGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCM351
Plasmid#46013DepositorTypeEmpty backboneUseCre/LoxTagsExpressionMutationPromoternaAvailable sinceJuly 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRSII416
Plasmid#35456DepositorTypeEmpty backboneUseYeast centromere vectorTagsExpressionMutationPromoterNoneAvailable sinceOct. 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLenti X2 Hygro DEST (w17-1)
Plasmid#17295Purpose3rd gen lentiviral Gateway destination vector, RNAi, 3’LTR insertion, HygroDepositorTypeEmpty backboneUseLentiviral and RNAiTagsExpressionMutationPromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
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pET21a-BirA
Plasmid#20857DepositorInsertBirA
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceApril 21, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAAV2/2
Plasmid#104963PurposeAAV packaging plasmid expressing Rep/Cap genes for production of serotype 2DepositorInsertRep2/Cap2
UseAAVTagsExpressionMammalianMutationPromoterp5Available sinceOct. 2, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBABE-puro-hTERT
Plasmid#1771PurposeRetroviral expression of hTERT. Used to create immortalized cell lines.DepositorInserthTERT (TERT Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 3, 2005AvailabilityAcademic Institutions and Nonprofits only -
pUMVC
Plasmid#8449PurposePackaging plasmid for producing MuLV retroviral particles. Use in conjunction with an envelope plasmid such as pCMV-VSV-G.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 4, 2005AvailabilityAcademic Institutions and Nonprofits only -
pLV-eGFP
Plasmid#36083Purpose3rd generation lentiviral transfer plasmidDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviralTagsEGFPExpressionMammalianMutationPromoterAvailable sinceJune 1, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSIV-D3psi/delta env/delta Vif/delta Vpr
Plasmid#132928PurposeProduction of SIV-VLPs containing Vpx proteinDepositorInsertGag, pol, and vpx
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMXs-3XHA-EGFP-OMP25
Plasmid#83356PurposeFor tagging mitochondria with HA epitopes (HA-MITO construct)DepositorInsert3XHA-EGFP-OMP25
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
Tet-pLKO-puro
Plasmid#21915Purpose3rd generation lentiviral plasmid for inducible expression of shRNA; puromycin selection. See manual for detailed protocols. No further technical support is available from the depositing laboratory.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 22, 2009AvailabilityAcademic Institutions and Nonprofits only -
7M8
Plasmid#64839PurposeThis plasmid can be used in the triple transfection method of AAV vector production. It supplies the replication proteins from AAV2 and the 7M8 capsid protein.DepositorInsert7M8 cap
UseAAVTagsExpressionMutation7mer insertion in the AAV2 capPromoterAvailable sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET21b(+)-Is-PETase
Plasmid#112202PurposepET-21b(+) based plasmid for expression of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1), codon optimized for expression in E. coli K12DepositorInsertPETase gene from Ideonella sakaiensis 201-F6, codon optimized for expression in E. coli K12
UseTags6XHISExpressionBacterialMutationPromoterN/AAvailable sinceJuly 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
CROPseq-Guide-Puro
Plasmid#86708PurposeMakes CRISPR gRNAs detectable by single-cell RNA-seq. The gRNA is expressed as part of the puromycin resistance mRNA transcribed by RNA Pol II and in its functional form from the hU6 promoter.DepositorInsertEF1a-Puro-WPRE-hU6-gRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceFeb. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUCmini-iCAP-AAV9-X1.1
Plasmid#196836Purposenon-standard AAV2 rep-AAV9-X1.1 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification systemDepositorInsertSynthetic construct isolate AAV9-X1.1 VP1 gene
UseAAVTagsExpressionMammalianMutationPromoterp41Available sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLminP_Luc2P_RE1
Plasmid#90335PurposeNFkB - p50/p65 pathway gene reporterDepositorInsertFirefly luciferase
UseLentiviralTagsExpressionMutationPromoterNFKBAvailable sinceAug. 22, 2022AvailabilityAcademic Institutions and Nonprofits only